FastQCFastQC Report
Fri 27 May 2016
SRR2138878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6138945
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85550.044.4233741
GTATCAA152150.036.359491
TCAACGC168850.032.2372634
ATCAACG168950.032.1184353
CAACGCA170750.031.8291995
AACGCAG176050.030.990136
TATCAAC179250.030.4863782
ACGCAGA200400.027.1825667
CGCAGAG204500.026.5963828
GCAGAGT244200.022.15189
GTACATG181450.020.4338741
GAGTACT142550.020.34821712-13
TACATGG177650.020.2068862
ACATGGG176900.019.79113
CAGAGTA234100.019.41823210-11
CATGGGG135200.019.2578834
AGGACCG13450.018.4810415
GTACTTT158150.018.27468314-15
AGAGTAC215650.018.13936610-11
GTGGTAT47150.016.7996861
Produced by FastQC (version 0.11.5)