##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6138945
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.091086171972545	28.0	18.0	32.0	18.0	33.0
2	26.60904846028104	28.0	18.0	32.0	18.0	33.0
3	29.74279033286664	32.0	27.0	33.0	25.0	33.0
4	30.830618616065138	33.0	31.0	33.0	27.0	33.0
5	32.368597861684705	33.0	33.0	33.0	32.0	34.0
6	36.565421583024445	38.0	37.0	38.0	34.0	38.0
7	36.75482953504226	38.0	37.0	38.0	34.0	38.0
8	37.04516736996341	38.0	38.0	38.0	36.0	38.0
9	37.15332846278962	38.0	38.0	38.0	36.0	38.0
10-11	37.16979546159804	38.0	38.0	38.0	36.0	38.0
12-13	37.225475631399206	38.0	38.0	38.0	36.0	38.0
14-15	37.21724710027537	38.0	38.0	38.0	36.0	38.0
16-17	37.197838390798424	38.0	38.0	38.0	36.0	38.0
18-19	37.21814986125466	38.0	38.0	38.0	36.0	38.0
20-21	37.23465754921241	38.0	38.0	38.0	36.0	38.0
22-23	37.24872753113412	38.0	38.0	38.0	36.0	38.0
24-25	37.246002970290746	38.0	38.0	38.0	36.0	38.0
26-27	37.20425080499142	38.0	38.0	38.0	36.0	38.0
28-29	37.179546685244404	38.0	38.0	38.0	36.0	38.0
30-31	37.18677832819705	38.0	38.0	38.0	36.0	38.0
32-33	37.17663492922192	38.0	38.0	38.0	36.0	38.0
34-35	37.15533850018906	38.0	38.0	38.0	36.0	38.0
36-37	37.14013140675341	38.0	38.0	38.0	36.0	38.0
38-39	37.124763068853355	38.0	38.0	38.0	36.0	38.0
40-41	37.10746693708151	38.0	38.0	38.0	36.0	38.0
42-43	37.0796051755768	38.0	38.0	38.0	36.0	38.0
44-45	37.039428972424574	38.0	38.0	38.0	36.0	38.0
46-47	37.019896707491725	38.0	38.0	38.0	36.0	38.0
48-49	36.98884736791101	38.0	38.0	38.0	36.0	38.0
50-51	36.95734564214081	38.0	38.0	38.0	36.0	38.0
52-53	36.93933429572337	38.0	38.0	38.0	36.0	38.0
54-55	36.91321442091545	38.0	38.0	38.0	36.0	38.0
56-57	36.91614132661506	38.0	38.0	38.0	36.0	38.0
58-59	36.90343187295055	38.0	38.0	38.0	36.0	38.0
60-61	36.91915608653785	38.0	38.0	38.0	36.0	38.0
62-63	36.91391998434407	38.0	38.0	38.0	36.0	38.0
64-65	36.90854806967194	38.0	38.0	38.0	35.5	38.0
66-67	36.90096201781053	38.0	38.0	38.0	35.0	38.0
68-69	36.91019610663214	38.0	38.0	38.0	35.0	38.0
70-71	36.89332940901774	38.0	38.0	38.0	35.0	38.0
72-73	36.88061624548549	38.0	38.0	38.0	35.0	38.0
74-75	36.89717242927789	38.0	38.0	38.0	35.0	38.0
76-77	36.88219608348736	38.0	38.0	38.0	35.0	38.0
78-79	36.85740153365407	38.0	38.0	38.0	35.0	38.0
80-81	36.851044066980535	38.0	38.0	38.0	35.0	38.0
82-83	36.800808334235924	38.0	38.0	38.0	35.0	38.0
84-85	36.80639065459907	38.0	38.0	38.0	35.0	38.0
86-87	36.79768448181383	38.0	38.0	38.0	35.0	38.0
88-89	36.778825752477566	38.0	38.0	38.0	35.0	38.0
90-91	36.75939970021769	38.0	38.0	38.0	35.0	38.0
92-93	36.765218021118315	38.0	38.0	38.0	35.0	38.0
94-95	36.71065300540707	38.0	38.0	38.0	34.5	38.0
96-97	36.67844740534267	38.0	38.0	38.0	34.0	38.0
98-99	36.70638814843305	38.0	38.0	38.0	34.0	38.0
100	35.13956395279582	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	12.0
19	36.0
20	75.0
21	190.0
22	593.0
23	1550.0
24	3794.0
25	7537.0
26	13568.0
27	21553.0
28	32792.0
29	47176.0
30	63841.0
31	83905.0
32	109728.0
33	148500.0
34	219664.0
35	407808.0
36	1243157.0
37	3733466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.58611536672832	17.306605613831042	13.258809127626977	22.848469891813657
2	14.504368666020712	19.8241293456156	41.63750204440147	24.033999943962222
3	18.760389610918487	25.497801332313614	30.434431323297407	25.307377733470492
4	12.524432129624879	17.546826042585494	39.80296288694556	30.12577894084407
5	13.321844682651953	37.58994411296518	35.34750697691996	13.740704227462903
6	29.738370184694723	37.82101831569973	18.905492005868766	13.535119493736786
7	26.237814477894815	33.10435262084935	23.28281162316978	17.375021278086056
8	23.994660320299335	36.85573009694663	21.409199789214597	17.740409793539445
9	25.692509054894614	17.117452591609798	21.527819519477696	35.66221883401789
10-11	24.102064768457772	26.76853270390922	29.00835567023324	20.121046857399765
12-13	24.932744958620738	24.40799681378478	29.15632083362858	21.5029373939659
14-15	22.211552636487212	25.668156336308602	26.88163031270031	25.238660714503876
16-17	21.629783293383472	28.819088621904903	27.551753599356243	21.999374485355382
18-19	21.66592956933154	27.973853813643874	29.293779305727615	21.066437311296973
20-21	22.48430644045179	27.169234693811063	29.17654912406676	21.16990974167039
22-23	22.23500664774742	26.965182550165995	29.05603615709943	21.743774644987155
24-25	22.00921333600736	27.053837377581413	29.160545703454467	21.776403582956764
26-27	21.896161093771767	27.247655522506253	29.095953510463485	21.760229873258496
28-29	21.841188158836292	27.34546942314996	29.1277076775873	21.685634740426448
30-31	22.18202104298098	27.13715047256422	28.98567942463447	21.69514905982033
32-33	21.91719731833219	27.32423711759564	28.945479554536433	21.81308600953574
34-35	22.000229773754914	27.32498215150297	28.961356978146874	21.713431096595244
36-37	22.049475208599482	27.226073152141065	28.970678380638965	21.753773258620484
38-39	21.853421916012678	27.424730065411122	28.97386177941359	21.747986239162607
40-41	22.116656074470512	27.257038696200848	28.83797518457888	21.788330044749767
42-43	22.065600196239902	27.287084422244657	28.71557714883368	21.93173823268176
44-45	22.08297378682905	27.43282181274665	28.578529460604607	21.90567493981969
46-47	22.114713074816937	27.39931540103874	28.408273078690083	22.07769844545424
48-49	22.034713151517398	27.520900810997585	28.383450434278473	22.06093560320654
50-51	22.027352680866493	27.63499522126873	28.285242136966033	22.052409960898743
52-53	22.226831325813748	27.542917471960422	28.122755452358707	22.107495749867127
54-55	22.221284208735902	27.412316146759334	28.183790681696845	22.182608962807915
56-57	22.165572186294042	27.572875613911457	28.145444513752093	22.116107686042405
58-59	22.101550620197223	27.598749143609822	28.198058800494323	22.101641435698628
60-61	22.20311985729809	27.556083048609054	28.196392853569595	22.04440424052326
62-63	22.17352141666325	27.561436109418125	28.187992721851728	22.077049752066895
64-65	22.140645479753264	27.547188156170126	28.173446740721765	22.13871962335484
66-67	22.2362008432141	27.529538378636158	28.04066654498748	22.193594233162266
68-69	22.181735522232106	27.5153019000287	28.122430481833753	22.180532095905438
70-71	22.193449795027842	27.298212108157156	28.154335067694554	22.354003029120445
72-73	22.29698108602807	27.368502745576574	28.069906040268457	22.264610128126908
74-75	22.117085380239722	27.515324792474814	28.013744319976574	22.353845507308883
76-77	22.258982967273973	27.435213467085575	27.987279561027968	22.318524004612485
78-79	22.25112198999515	27.507230265938613	27.896560037687163	22.345087706379076
80-81	22.185134505707495	27.58107918893665	27.879098557236006	22.35468774811985
82-83	22.280310528170226	27.516248489019823	27.95765239646179	22.245788586348162
84-85	22.419930980455042	27.492479174705302	27.83324438631569	22.254345458523964
86-87	22.2083098458581	27.50159299466735	27.90567612987516	22.384421029599395
88-89	22.26970766333726	27.516356703309384	27.879485135224673	22.334450498128678
90-91	22.318992129078737	27.54809457397574	27.857192244879453	22.275721052066068
92-93	22.342487745632393	27.517477989685535	27.85016618524182	22.289868079440247
94-95	22.246744559801535	27.56680934287004	27.820494565211963	22.365951532116462
96-97	22.35062115965239	27.696126355308	27.87442997344253	22.078822511597078
98-99	23.382258264616	29.612111966983377	28.083984780759096	18.921644987641525
100	22.2421156322151	30.48378793544384	22.069600579227966	25.20449585311309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1115.0
1	1039.5
2	1495.0
3	2220.5
4	2809.5
5	3955.5
6	5069.0
7	6186.0
8	7939.5
9	9047.5
10	8740.5
11	8441.0
12	9198.0
13	10999.0
14	14098.5
15	17572.0
16	20651.5
17	23320.5
18	23967.5
19	22431.5
20	20791.5
21	20090.0
22	21096.5
23	24423.0
24	30528.0
25	39833.5
26	52204.5
27	65686.5
28	78722.5
29	93556.5
30	107708.5
31	120624.5
32	134634.0
33	148378.0
34	162981.5
35	177302.5
36	192086.0
37	205624.0
38	213946.0
39	221133.5
40	231024.5
41	239163.0
42	244984.5
43	251313.0
44	255216.0
45	258261.0
46	259619.0
47	259012.0
48	258517.5
49	254755.5
50	248989.0
51	241742.5
52	231664.5
53	220192.0
54	208343.5
55	194628.0
56	179367.0
57	162990.0
58	145536.0
59	127653.0
60	108573.0
61	89131.0
62	70712.5
63	55310.5
64	42625.5
65	31971.5
66	23636.0
67	18059.0
68	13946.0
69	10234.0
70	7421.0
71	5460.5
72	3905.0
73	2652.0
74	1814.5
75	1211.0
76	766.5
77	463.5
78	306.5
79	202.5
80	126.5
81	85.5
82	58.0
83	40.5
84	27.0
85	14.0
86	8.0
87	4.5
88	4.5
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003730282646285315
3	0.0
4	0.0
5	0.0021664960347421262
6	6.515777548096618E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.145251334762336E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.453911453059128E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.7168192489774197E-5
48-49	3.6407899543323583E-4
50-51	0.0016622919226085876
52-53	0.003768535657363679
54-55	0.005698997644265895
56-57	0.006374858206609425
58-59	0.008091006478526097
60-61	0.00785858868550295
62-63	0.005573196295657508
64-65	0.0031878451298093338
66-67	0.0010391748120652353
68-69	5.921586542029368E-4
70-71	2.607474296097828E-4
72-73	5.076240247517474E-4
74-75	0.0
76-77	2.9920029744498906E-4
78-79	2.9253867991819814E-4
80-81	6.123262498752385E-5
82-83	1.0323546129836762E-5
84-85	6.263870558367685E-5
86-87	1.0560112596144544E-4
88-89	1.068716207861455E-5
90-91	1.0820473200934023E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	75.0
20-21	227.0
22-23	468.0
24-25	599.0
26-27	1804.0
28-29	4600.0
30-31	14282.0
32-33	18389.0
34-35	25083.0
36-37	38108.0
38-39	44494.0
40-41	43644.0
42-43	49645.0
44-45	57900.0
46-47	57905.0
48-49	54054.0
50-51	50110.0
52-53	49466.0
54-55	53424.0
56-57	55008.0
58-59	53665.0
60-61	52332.0
62-63	51803.0
64-65	54991.0
66-67	57236.0
68-69	57970.0
70-71	56448.0
72-73	53419.0
74-75	54496.0
76-77	56393.0
78-79	57272.0
80-81	56635.0
82-83	54145.0
84-85	54285.0
86-87	55860.0
88-89	57352.0
90-91	57209.0
92-93	57354.0
94-95	66529.0
96-97	188777.0
98-99	403797.0
100-101	3861692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88140516710327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82521599169658	78.91022389964257
2	6.560684072239383	11.400029021071816
3	1.499168288579261	3.907495424811827
4	0.5316667513452352	1.8476781775001168
5	0.22979567741232276	0.9982485677454485
6	0.1175601349638758	0.6128273830377502
7	0.06741161986668287	0.4099771381025615
8	0.045398173289893984	0.3155405669956516
9	0.032170094458985096	0.2515484709859561
>10	0.08896811767936588	1.152270578725543
>50	0.0013826960856292002	0.07821806353319263
>100	4.940623966628735E-4	0.06949689838606894
>500	8.43199861434349E-5	0.04644580946145347
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.8868331610724645E-5	0.0	0.0	0.0
3	0.0	4.8868331610724645E-5	0.0	0.0	0.0
4	0.0	6.51577754809662E-5	0.0	0.0	0.0
5	0.0	6.51577754809662E-5	0.0	0.0	0.0
6	0.0	6.51577754809662E-5	0.0	0.0	0.0
7	0.0	6.51577754809662E-5	0.0	0.0	0.0
8	0.0	9.773666322144929E-5	0.0	0.0	0.0
9	0.0	1.303155509619324E-4	0.0	0.0	0.0
10-11	0.0	1.303155509619324E-4	0.0	0.0	0.0
12-13	0.0	1.6289443870241548E-4	0.0	0.0	0.0
14-15	0.0	1.9547332644289858E-4	0.0	0.0	2.4434165805362323E-5
16-17	0.0	1.9547332644289858E-4	0.0	1.628944387024155E-5	3.25788877404831E-5
18-19	0.0	1.9547332644289858E-4	0.0	4.0723609675603876E-5	3.25788877404831E-5
20-21	0.0	1.9547332644289858E-4	0.0	4.8868331610724645E-5	3.25788877404831E-5
22-23	0.0	1.9547332644289858E-4	0.0	6.51577754809662E-5	3.25788877404831E-5
24-25	0.0	2.606311019238648E-4	0.0	9.773666322144929E-5	3.25788877404831E-5
26-27	0.0	3.0949943353458944E-4	0.0	1.3031555096193237E-4	3.25788877404831E-5
28-29	0.0	3.0949943353458944E-4	0.0	3.5836776514531403E-4	3.25788877404831E-5
30-31	0.0	3.420783212750725E-4	0.0	0.0010262349638252176	3.25788877404831E-5
32-33	0.0	3.420783212750725E-4	0.0	0.002875086843097633	3.25788877404831E-5
34-35	0.0	4.4795970643164257E-4	0.0	0.005131174819126088	3.25788877404831E-5
36-37	0.0	5.456963696530918E-4	0.0	0.008462366090590484	3.25788877404831E-5
38-39	0.0	5.701305354584542E-4	0.0	0.013617975075521933	3.25788877404831E-5
40-41	0.0	6.352883109394204E-4	0.0	0.018789873504323623	3.25788877404831E-5
42-43	0.0	6.515777548096619E-4	0.0	0.024140955815697973	3.25788877404831E-5
44-45	0.0	6.84156642550145E-4	0.0	0.030127326438011744	3.25788877404831E-5
46-47	0.0	7.004460864203865E-4	0.0	0.036439485937730345	3.25788877404831E-5
48-49	0.0	7.004460864203865E-4	0.0	0.04258060627681141	3.25788877404831E-5
50-51	0.0	7.737485838364735E-4	0.0	0.0486565688404115	3.25788877404831E-5
52-53	0.0	7.981827496418358E-4	0.0	0.05533524082721054	3.25788877404831E-5
54-55	0.0	8.144721935120774E-4	0.0	0.06153337421983744	3.25788877404831E-5
56-57	0.0	8.470510812525605E-4	0.0	0.06745458706667025	3.25788877404831E-5
58-59	0.0	8.470510812525605E-4	0.0	0.07408439072185857	3.25788877404831E-5
60-61	0.0	8.470510812525605E-4	0.0	0.07973682774483237	3.25788877404831E-5
62-63	0.0	8.470510812525605E-4	0.0	0.0852752386607145	3.25788877404831E-5
64-65	0.0	8.63340525122802E-4	0.0	0.0912697540049634	3.25788877404831E-5
66-67	0.0	8.796299689930436E-4	0.0	0.09710951963244499	3.25788877404831E-5
68-69	0.0	9.122088567335266E-4	0.0	0.10239544416833837	3.25788877404831E-5
70-71	0.0	9.284983006037683E-4	0.0	0.10714381705651378	3.25788877404831E-5
72-73	0.0	9.447877444740098E-4	0.0	0.1117374402279219	3.25788877404831E-5
74-75	0.0	0.0010099455199549762	0.0	0.11529668371356969	3.25788877404831E-5
76-77	0.0	0.0010995374612413045	0.0	0.11881520358954184	3.25788877404831E-5
78-79	0.0	0.0011239716270466668	0.0	0.12107129156557031	3.25788877404831E-5
80-81	0.0	0.0011239716270466668	0.0	0.12183689542747166	3.25788877404831E-5
82-83	0.0	0.0011239716270466668	0.0	0.12233372346551402	3.25788877404831E-5
84-85	0.0	0.0011239716270466668	0.0	0.12259435456743789	3.25788877404831E-5
86-87	0.0	0.0012217082902681161	0.0	0.12278168317194567	3.25788877404831E-5
88	0.0	0.0012542871780085992	0.0	0.12280611733775103	3.25788877404831E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8555	0.0	44.423374	1
GTATCAA	15215	0.0	36.35949	1
TCAACGC	16885	0.0	32.237263	4
ATCAACG	16895	0.0	32.118435	3
CAACGCA	17075	0.0	31.829199	5
AACGCAG	17605	0.0	30.99013	6
TATCAAC	17925	0.0	30.486378	2
ACGCAGA	20040	0.0	27.182566	7
CGCAGAG	20450	0.0	26.596382	8
GCAGAGT	24420	0.0	22.1518	9
GTACATG	18145	0.0	20.433874	1
GAGTACT	14255	0.0	20.348217	12-13
TACATGG	17765	0.0	20.206886	2
ACATGGG	17690	0.0	19.7911	3
CAGAGTA	23410	0.0	19.418232	10-11
CATGGGG	13520	0.0	19.257883	4
AGGACCG	1345	0.0	18.481041	5
GTACTTT	15815	0.0	18.274683	14-15
AGAGTAC	21565	0.0	18.139366	10-11
GTGGTAT	4715	0.0	16.799686	1
>>END_MODULE