FastQCFastQC Report
Fri 27 May 2016
SRR2138877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686892
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.047.6702841
GTATCAA97850.036.4561771
ATCAACG110050.031.5258033
TCAACGC111100.031.2660774
CAACGCA113550.030.5914675
TATCAAC114500.030.561482
AACGCAG117650.029.523496
ACGCAGA136200.025.564847
CGCAGAG140100.024.8531868
GTGGTAT26600.022.510761
GTACATG130750.021.7613491
TACATGG126250.021.5615182
GCAGAGT167150.020.8057739
ACATGGG130700.020.3391553
TGGTATC26000.019.6002032
GAGTACT99850.019.4345512-13
CATGGGG103100.019.111444
GCGTACA4950.018.874271
CAGAGTA162300.018.66731510-11
AGAGTAC148800.017.47871610-11
Produced by FastQC (version 0.11.5)