##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4686892
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.095720362235785	28.0	18.0	32.0	18.0	33.0
2	26.632109722178363	28.0	18.0	32.0	18.0	33.0
3	29.76173698903239	32.0	27.0	33.0	25.0	33.0
4	30.831476168002165	33.0	31.0	33.0	27.0	33.0
5	32.349632336311565	33.0	33.0	33.0	32.0	34.0
6	36.55080552314839	38.0	37.0	38.0	34.0	38.0
7	36.76645888149332	38.0	37.0	38.0	34.0	38.0
8	37.02756581547004	38.0	38.0	38.0	36.0	38.0
9	37.14334381931566	38.0	38.0	38.0	36.0	38.0
10-11	37.16050967250792	38.0	38.0	38.0	36.0	38.0
12-13	37.21284360723482	38.0	38.0	38.0	36.0	38.0
14-15	37.200913206448966	38.0	38.0	38.0	36.0	38.0
16-17	37.1755947224728	38.0	38.0	38.0	36.0	38.0
18-19	37.19727241421394	38.0	38.0	38.0	36.0	38.0
20-21	37.215076063126375	38.0	38.0	38.0	36.0	38.0
22-23	37.22429638787021	38.0	38.0	38.0	36.0	38.0
24-25	37.22161944779499	38.0	38.0	38.0	36.0	38.0
26-27	37.182525632239816	38.0	38.0	38.0	36.0	38.0
28-29	37.15957539742794	38.0	38.0	38.0	36.0	38.0
30-31	37.16414251914638	38.0	38.0	38.0	36.0	38.0
32-33	37.15508499961402	38.0	38.0	38.0	36.0	38.0
34-35	37.13266004715214	38.0	38.0	38.0	36.0	38.0
36-37	37.11446748266815	38.0	38.0	38.0	36.0	38.0
38-39	37.10115981249537	38.0	38.0	38.0	36.0	38.0
40-41	37.08310497375765	38.0	38.0	38.0	36.0	38.0
42-43	37.05524170561442	38.0	38.0	38.0	36.0	38.0
44-45	37.01344193896661	38.0	38.0	38.0	36.0	38.0
46-47	36.995040330661524	38.0	38.0	38.0	36.0	38.0
48-49	36.9621217321823	38.0	38.0	38.0	36.0	38.0
50-51	36.92792451429387	38.0	38.0	38.0	36.0	38.0
52-53	36.906693128182056	38.0	38.0	38.0	36.0	38.0
54-55	36.87984125777941	38.0	38.0	38.0	35.5	38.0
56-57	36.88046338774224	38.0	38.0	38.0	35.0	38.0
58-59	36.867477179666096	38.0	38.0	38.0	35.0	38.0
60-61	36.87518457481765	38.0	38.0	38.0	35.0	38.0
62-63	36.86784409345604	38.0	38.0	38.0	35.0	38.0
64-65	36.86045910589023	38.0	38.0	38.0	35.0	38.0
66-67	36.850742419094146	38.0	38.0	38.0	35.0	38.0
68-69	36.85725438710192	38.0	38.0	38.0	35.0	38.0
70-71	36.83607708509426	38.0	38.0	38.0	35.0	38.0
72-73	36.81999821420631	38.0	38.0	38.0	35.0	38.0
74-75	36.83508647904652	38.0	38.0	38.0	35.0	38.0
76-77	36.81720364774042	38.0	38.0	38.0	35.0	38.0
78-79	36.79066593564528	38.0	38.0	38.0	35.0	38.0
80-81	36.78375667047757	38.0	38.0	38.0	35.0	38.0
82-83	36.726721364472596	38.0	38.0	38.0	35.0	38.0
84-85	36.72851351467402	38.0	38.0	38.0	35.0	38.0
86-87	36.71761014675313	38.0	38.0	38.0	35.0	38.0
88-89	36.696163215595305	38.0	38.0	38.0	34.5	38.0
90-91	36.67554142834726	38.0	38.0	38.0	34.0	38.0
92-93	36.67748514743086	38.0	38.0	38.0	34.0	38.0
94-95	36.62271220685088	38.0	38.0	38.0	34.0	38.0
96-97	36.58977309041375	38.0	38.0	38.0	34.0	38.0
98-99	36.618807196860644	38.0	38.0	38.0	34.0	38.0
100	35.02343758010004	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	10.0
19	21.0
20	57.0
21	162.0
22	495.0
23	1384.0
24	3242.0
25	6535.0
26	11361.0
27	18207.0
28	26702.0
29	38128.0
30	51002.0
31	67206.0
32	87548.0
33	116660.0
34	171739.0
35	308133.0
36	928167.0
37	2850131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.92275819455622	16.775914614631617	13.40389324097931	22.897433949832852
2	14.627079677173294	19.55000560092602	41.56853666472857	24.25437805717212
3	18.98140601490284	25.080181066685558	30.17302297556675	25.765389942844852
4	12.62186113953554	17.298841108350693	39.53933651554164	30.53996123657213
5	13.568237084628548	37.38278513757471	35.169675507739925	13.879302270056812
6	30.090194156507653	37.59845816702721	18.735732806985613	13.575614869479521
7	26.566709879382756	32.84470817761536	23.01996717654258	17.56861476645931
8	24.14147797730351	36.82798750216561	21.23921353425682	17.79132098627406
9	25.72214166658843	17.14861362284431	21.30330718096342	35.82593752960384
10-11	24.26094947355305	26.635390787754442	28.90304491761278	20.200614821079725
12-13	25.019127814338372	24.175818431489354	29.013053853171783	21.79199990100049
14-15	22.317678751718624	25.515896248516075	26.623133197863318	25.543291801901987
16-17	21.905956015201543	28.65911994558441	27.27214537906997	22.162778660144077
18-19	21.907897600371417	27.82511310267017	29.00529818054267	21.26169111641574
20-21	22.67326557334936	27.103641539059286	28.798003856332105	21.425089031259247
22-23	22.43805261678899	26.873656164631715	28.71773381535198	21.970557403227318
24-25	22.193549364532323	26.967190744434255	28.873199013910707	21.966060877122715
26-27	22.097891890230787	27.168162215871263	28.774757229341446	21.95918866455651
28-29	22.04617241550093	27.26367287234497	28.77266714941276	21.917487562741336
30-31	22.37244921945453	27.046575865874225	28.67009805220504	21.910876862466207
32-33	22.173037802767862	27.19854015864777	28.625988898275313	22.00243314030906
34-35	22.236273276637757	27.24366529343144	28.599732841441426	21.920328588489372
36-37	22.291307188619175	27.09539868458668	28.643437281057853	21.969856845736295
38-39	22.06664835322208	27.30185243529756	28.71179238282216	21.919706828658196
40-41	22.364945750280274	27.131717930724342	28.55406811197172	21.949268207023668
42-43	22.242385538611018	27.222928677303916	28.402433215259386	22.132252568825685
44-45	22.234946701238837	27.351871551093677	28.36112403873856	22.052057708928928
46-47	22.321493160736967	27.243700799418775	28.136960352634706	22.297845687209556
48-49	22.23347875200593	27.371494886958548	28.164886590807107	22.230139770228423
50-51	22.23578249350146	27.498065111445484	28.057533416355714	22.20861897869734
52-53	22.430171953213318	27.464085648705478	27.89655978922604	22.209182608855166
54-55	22.425312936464167	27.29520550739973	27.933440886850686	22.34604066928542
56-57	22.366072678079956	27.449246629659	27.923088585156503	22.261592107104537
58-59	22.302406068460343	27.462202448090256	28.004920233312163	22.23047125013724
60-61	22.409156265247095	27.39236212412074	28.01398342161137	22.1844981890208
62-63	22.308115091173423	27.479344981220965	27.987981706855425	22.224558220750186
64-65	22.32364167285086	27.40469289732142	27.961064954591492	22.310600475236228
66-67	22.43402212232628	27.43390802178088	27.818335335279265	22.313734520613576
68-69	22.35191046173714	27.416075753283586	27.90698935825833	22.325024426720947
70-71	22.369945889638878	27.18123091450583	27.959153757641825	22.48966943821347
72-73	22.39753071043655	27.258576560494006	27.88362334402042	22.460269385049024
74-75	22.243886761461905	27.429292605298105	27.882389033105078	22.444431600134905
76-77	22.403392126827747	27.337621099494935	27.75472037320273	22.504266400474588
78-79	22.391158503649006	27.46015909516231	27.668129000998736	22.480553400189944
80-81	22.4114019410471	27.397267097550216	27.645082377443188	22.546248583959503
82-83	22.478298696789064	27.39037597662654	27.715612339029594	22.415712987554798
84-85	22.58823759348452	27.336575865850023	27.65999914057226	22.4151874000932
86-87	22.332050809743247	27.388496684735824	27.764189194034106	22.515263311486823
88-89	22.43332718212178	27.414714181378848	27.666608346432586	22.485350290066787
90-91	22.513518898783992	27.37866200262352	27.714337504773816	22.393481593818667
92-93	22.480217878068633	27.416729603153662	27.657033826116113	22.446018692661593
94-95	22.402227417274585	27.435766490174068	27.6592563958611	22.50274969669025
96-97	22.49456517431433	27.589430534028818	27.70221262435969	22.213791667297155
98-99	23.546778281554506	29.49299669863543	27.860563398750767	19.099661621059298
100	22.425198350422995	30.36295258276411	21.876871756930484	25.33497730988241
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	739.0
1	711.5
2	1071.5
3	1612.0
4	2003.0
5	2740.5
6	3448.5
7	4175.5
8	5366.0
9	6137.5
10	5979.0
11	5836.0
12	6398.0
13	7707.0
14	9928.5
15	12305.5
16	14308.5
17	15976.0
18	16397.5
19	15401.0
20	14272.5
21	13725.5
22	14367.5
23	16865.5
24	21283.0
25	27780.0
26	36725.5
27	46657.5
28	56024.0
29	66091.0
30	75676.5
31	84878.5
32	95007.5
33	105283.0
34	116317.5
35	126958.0
36	138085.0
37	148646.0
38	156153.0
39	162832.5
40	171825.0
41	179527.5
42	184571.0
43	189436.5
44	193699.5
45	197863.0
46	200179.0
47	201151.5
48	200658.0
49	197728.0
50	194274.0
51	189006.0
52	181650.0
53	173093.5
54	163449.0
55	152244.0
56	140056.0
57	127708.5
58	114314.5
59	100225.5
60	85096.0
61	69761.0
62	55277.5
63	42915.0
64	32778.5
65	24385.5
66	17975.5
67	13797.5
68	10711.5
69	7780.5
70	5636.0
71	4104.0
72	2856.5
73	1895.0
74	1226.0
75	785.5
76	511.5
77	330.0
78	221.0
79	140.0
80	82.0
81	53.5
82	35.0
83	23.5
84	17.5
85	12.5
86	8.0
87	5.5
88	5.0
89	5.5
90	4.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00356312882822988
3	0.0
4	0.0
5	0.001984257371409454
6	6.400830230353079E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.14165987849721E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.236167209403083E-5
48-49	5.188931242600133E-4
50-51	0.001854091633303444
52-53	0.004402395453426095
54-55	0.006068156142788684
56-57	0.006961776813365026
58-59	0.008307175340350033
60-61	0.007990634454001016
62-63	0.005821688160195609
64-65	0.0035774387375702133
66-67	0.0011226879492505998
68-69	6.41020109540475E-4
70-71	2.615404082421595E-4
72-73	6.415985314187026E-4
74-75	1.2703161664500355E-5
76-77	3.5930155370974366E-4
78-79	2.8531584399085275E-4
80-81	1.4419276107770717E-4
82-83	1.324845489894741E-5
84-85	1.3386707026695905E-4
86-87	1.2176270181153135E-4
88-89	2.7358976445973205E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	124.0
22-23	259.0
24-25	390.0
26-27	1162.0
28-29	3101.0
30-31	9845.0
32-33	12144.0
34-35	17007.0
36-37	26406.0
38-39	30602.0
40-41	29669.0
42-43	34163.0
44-45	39779.0
46-47	40114.0
48-49	37652.0
50-51	34717.0
52-53	34561.0
54-55	37846.0
56-57	38304.0
58-59	38244.0
60-61	37566.0
62-63	36805.0
64-65	38928.0
66-67	41188.0
68-69	41317.0
70-71	40772.0
72-73	38598.0
74-75	38965.0
76-77	40878.0
78-79	41218.0
80-81	40678.0
82-83	39084.0
84-85	39175.0
86-87	40135.0
88-89	41471.0
90-91	41945.0
92-93	41962.0
94-95	49161.0
96-97	146834.0
98-99	320522.0
100-101	3023563.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.67525599381231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.86973643556047	81.4657239650739
2	5.9577666693445845	10.566129691110673
3	1.304293999876205	3.4697581299064773
4	0.4293134500347265	1.522779203336645
5	0.18639165447144537	0.8264163837682816
6	0.09397378726368232	0.4999889785389053
7	0.04907639754313734	0.30463034807742645
8	0.029926157605521513	0.2122967749264635
9	0.02111564297143581	0.16851915413694132
>10	0.056380682294723795	0.7180739383207729
>50	0.0013494291548517415	0.08235302597576065
>100	6.090486196695525E-4	0.11294948686268226
>500	3.3322631539284644E-5	0.015690481204129154
>1k	3.3322628008893956E-5	0.03469043876094346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1336100767843594E-5	0.0	0.0	0.0
3	0.0	2.1336100767843594E-5	0.0	0.0	0.0
4	0.0	1.0668050383921798E-4	0.0	0.0	0.0
5	0.0	1.0668050383921798E-4	0.0	0.0	0.0
6	2.1336100767843594E-5	1.0668050383921798E-4	0.0	0.0	0.0
7	2.1336100767843594E-5	1.0668050383921798E-4	0.0	0.0	0.0
8	2.1336100767843594E-5	1.4935270537490516E-4	0.0	0.0	2.1336100767843594E-5
9	2.1336100767843594E-5	2.1336100767843595E-4	0.0	0.0	2.1336100767843594E-5
10-11	2.1336100767843594E-5	2.1336100767843595E-4	0.0	0.0	2.1336100767843594E-5
12-13	2.1336100767843594E-5	2.1336100767843595E-4	0.0	0.0	2.1336100767843594E-5
14-15	3.2004151151765394E-5	2.4536515883020137E-4	0.0	0.0	4.267220153568719E-5
16-17	8.534440307137437E-5	2.5603320921412315E-4	0.0	0.0	6.400830230353079E-5
18-19	8.534440307137437E-5	2.8803736036588854E-4	0.0	0.0	7.467635268745258E-5
20-21	8.534440307137437E-5	3.2004151151765394E-4	0.0	1.0668050383921797E-5	8.534440307137437E-5
22-23	8.534440307137437E-5	3.307095619015757E-4	0.0	2.1336100767843594E-5	9.601245345529618E-5
24-25	1.0668050383921798E-4	3.627137130533411E-4	0.0	2.1336100767843594E-5	1.2801660460706158E-4
26-27	1.2801660460706158E-4	3.840498138211847E-4	0.0	3.2004151151765394E-5	1.2801660460706158E-4
28-29	1.4935270537490516E-4	3.840498138211847E-4	0.0	2.3469710844627954E-4	1.3868465499098336E-4
30-31	1.4935270537490516E-4	4.693942168925591E-4	0.0	9.814606353208053E-4	1.6002075575882697E-4
32-33	1.4935270537490516E-4	4.693942168925591E-4	0.0	0.0028910416540428073	1.7068880614274875E-4
34-35	1.4935270537490516E-4	5.440705695800116E-4	0.0	0.005216676637737758	1.7068880614274875E-4
36-37	1.4935270537490516E-4	6.400830230353079E-4	0.0	0.008907822070574702	1.7068880614274875E-4
38-39	1.4935270537490516E-4	6.400830230353079E-4	0.0	0.01418850701061599	1.7068880614274875E-4
40-41	1.4935270537490516E-4	7.680996276423695E-4	0.0	0.01936251144681806	1.7068880614274875E-4
42-43	1.4935270537490516E-4	8.107718291780566E-4	0.0	0.025016578150296613	1.7068880614274875E-4
44-45	1.4935270537490516E-4	9.067842826333528E-4	0.0	0.03103335856682851	1.7068880614274875E-4
46-47	1.4935270537490516E-4	9.601245345529617E-4	0.0	0.037530201250636885	1.7068880614274875E-4
48-49	1.6002075575882697E-4	9.601245345529617E-4	0.0	0.043525645566400935	1.7068880614274875E-4
50-51	1.7068880614274875E-4	0.0010348008872404144	0.0	0.049691778688307736	1.7068880614274875E-4
52-53	1.7068880614274875E-4	0.0010454689376243362	0.0	0.05611394501942865	1.7068880614274875E-4
54-55	1.7068880614274875E-4	0.0010668050383921797	0.0	0.0626961321063084	1.7068880614274875E-4
56-57	1.7068880614274875E-4	0.0010881411391600233	0.0	0.06992907026660738	1.7068880614274875E-4
58-59	1.7068880614274875E-4	0.001098809189543945	0.0	0.07602052703582673	1.7068880614274875E-4
60-61	1.8135685652667056E-4	0.0011201452903117886	0.0	0.08231467676234058	1.7068880614274875E-4
62-63	2.1336100767843595E-4	0.0011521494414635542	0.0	0.08889686384922033	1.7068880614274875E-4
64-65	2.1336100767843595E-4	0.0012801660460706158	0.0	0.09525502187803772	1.7068880614274875E-4
66-67	2.1336100767843595E-4	0.0012908340964545375	0.0	0.10152783550378375	1.8135685652667056E-4
68-69	2.1336100767843595E-4	0.0013441743483741464	0.0	0.10809935454027958	1.9202490691059236E-4
70-71	2.3469710844627954E-4	0.0013441743483741464	0.0	0.11328402702686556	1.9202490691059236E-4
72-73	2.773693099819667E-4	0.0013441743483741464	0.0	0.11690049610701506	2.1336100767843595E-4
74-75	2.773693099819667E-4	0.00136551044914199	0.0	0.11997289461758454	2.1336100767843595E-4
76-77	2.773693099819667E-4	0.0013761784995259118	0.0	0.12340800684120734	2.1336100767843595E-4
78-79	2.773693099819667E-4	0.0013868465499098336	0.0	0.12552028081722388	2.1336100767843595E-4
80-81	2.773693099819667E-4	0.0013868465499098336	0.0	0.12613902773949132	2.1336100767843595E-4
82-83	2.773693099819667E-4	0.0013868465499098336	0.0	0.12654441365408037	2.1336100767843595E-4
84-85	2.880373603658885E-4	0.0013868465499098336	0.0	0.12678977881291056	2.1336100767843595E-4
86-87	2.987054107498103E-4	0.0013975146002937556	0.0	0.12684311906483017	2.1336100767843595E-4
88	2.987054107498103E-4	0.001472190952981208	0.0	0.12684311906483017	2.1336100767843595E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5550	0.0	47.670284	1
GTATCAA	9785	0.0	36.456177	1
ATCAACG	11005	0.0	31.525803	3
TCAACGC	11110	0.0	31.266077	4
CAACGCA	11355	0.0	30.591467	5
TATCAAC	11450	0.0	30.56148	2
AACGCAG	11765	0.0	29.52349	6
ACGCAGA	13620	0.0	25.56484	7
CGCAGAG	14010	0.0	24.853186	8
GTGGTAT	2660	0.0	22.51076	1
GTACATG	13075	0.0	21.761349	1
TACATGG	12625	0.0	21.561518	2
GCAGAGT	16715	0.0	20.805773	9
ACATGGG	13070	0.0	20.339155	3
TGGTATC	2600	0.0	19.600203	2
GAGTACT	9985	0.0	19.43455	12-13
CATGGGG	10310	0.0	19.11144	4
GCGTACA	495	0.0	18.87427	1
CAGAGTA	16230	0.0	18.667315	10-11
AGAGTAC	14880	0.0	17.478716	10-11
>>END_MODULE