FastQCFastQC Report
Fri 27 May 2016
SRR2138876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193410
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.045.6690941
GTATCAA132800.037.233481
TCAACGC154150.031.0615834
ATCAACG155450.030.8015673
CAACGCA157300.030.4664485
AACGCAG162700.029.5831076
TATCAAC163600.029.5256822
ACGCAGA187650.025.5821117
CGCAGAG189700.025.3056568
GCAGAGT227300.021.0265549
GTACATG168100.020.7840181
TACATGG166750.020.216672
GAGTACT131750.019.45394112-13
GTGGTAT40750.019.2026541
ACATGGG168400.019.189713
AGAGTAC198450.018.48867210-11
CAGAGTA220100.018.34185410-11
TGGTATC37800.018.0157032
CATGGGG127700.017.5883164
GTACTTT149350.016.99156414-15
Produced by FastQC (version 0.11.5)