FastQCFastQC Report
Fri 27 May 2016
SRR2138875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4917345
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77700.051.9165761
GTATCAA152450.038.4442751
ATCAACG184500.031.5001893
TATCAAC186600.031.430422
TCAACGC185750.031.3762194
CAACGCA188600.030.8804115
AACGCAG194500.029.9829626
ACGCAGA222050.026.1893237
CGCAGAG224950.025.90628
GTGGTAT33750.023.6140651
GCAGAGT251650.023.141329
GGACCGA10050.022.7715266
TGGTATC33550.022.5366362
CCGAACC9450.022.0550759
GTACATG204150.021.4012051
TACATGG203700.021.0873322
ACATGGG205250.020.5497463
GAGTACT147250.020.27379412-13
TGCGCGA4701.337794E-719.8967894
ACCGAAC10750.019.7681068
Produced by FastQC (version 0.11.5)