##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4917345
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.068482077218498	32.0	25.0	33.0	18.0	33.0
2	31.730149094684226	33.0	32.0	33.0	27.0	33.0
3	31.33382262176032	33.0	31.0	33.0	29.0	33.0
4	32.496274310629005	33.0	33.0	33.0	31.0	34.0
5	33.13002606081127	33.0	33.0	34.0	33.0	34.0
6	37.301612760544565	38.0	38.0	38.0	36.0	38.0
7	37.352977470565925	38.0	38.0	38.0	37.0	38.0
8	37.53686145674139	38.0	38.0	38.0	37.0	38.0
9	37.575270801621606	38.0	38.0	38.0	38.0	38.0
10-11	37.5691560181358	38.0	38.0	38.0	37.5	38.0
12-13	37.597334638915925	38.0	38.0	38.0	38.0	38.0
14-15	37.593306143864226	38.0	38.0	38.0	38.0	38.0
16-17	37.61089276835365	38.0	38.0	38.0	38.0	38.0
18-19	37.6024743026979	38.0	38.0	38.0	38.0	38.0
20-21	37.59700897962986	38.0	38.0	38.0	38.0	38.0
22-23	37.601350700848315	38.0	38.0	38.0	38.0	38.0
24-25	37.55654365568266	38.0	38.0	38.0	38.0	38.0
26-27	37.553481775197156	38.0	38.0	38.0	38.0	38.0
28-29	37.54474172271935	38.0	38.0	38.0	38.0	38.0
30-31	37.53819783676273	38.0	38.0	38.0	38.0	38.0
32-33	37.53158187557423	38.0	38.0	38.0	38.0	38.0
34-35	37.51186329142175	38.0	38.0	38.0	38.0	38.0
36-37	37.48665203207588	38.0	38.0	38.0	38.0	38.0
38-39	37.46407380833399	38.0	38.0	38.0	37.5	38.0
40-41	37.4419193400351	38.0	38.0	38.0	37.5	38.0
42-43	37.417921640904524	38.0	38.0	38.0	37.0	38.0
44-45	37.38094199330391	38.0	38.0	38.0	37.0	38.0
46-47	37.33726368294039	38.0	38.0	38.0	37.0	38.0
48-49	37.289938989801925	38.0	38.0	38.0	37.0	38.0
50-51	37.273628968665555	38.0	38.0	38.0	37.0	38.0
52-53	37.26809998676008	38.0	38.0	38.0	37.0	38.0
54-55	37.25001265414426	38.0	38.0	38.0	37.0	38.0
56-57	37.22460190539274	38.0	38.0	38.0	37.0	38.0
58-59	37.233355436051724	38.0	38.0	38.0	37.0	38.0
60-61	37.25685138188602	38.0	38.0	38.0	37.0	38.0
62-63	37.266401595628324	38.0	38.0	38.0	37.0	38.0
64-65	37.27962173478727	38.0	38.0	38.0	37.0	38.0
66-67	37.29272279078316	38.0	38.0	38.0	37.0	38.0
68-69	37.29623131922148	38.0	38.0	38.0	37.0	38.0
70-71	37.27441685813792	38.0	38.0	38.0	37.0	38.0
72-73	37.26119509288554	38.0	38.0	38.0	37.0	38.0
74-75	37.25075582335074	38.0	38.0	38.0	37.0	38.0
76-77	37.258033670617706	38.0	38.0	38.0	37.0	38.0
78-79	37.26338774596306	38.0	38.0	38.0	37.0	38.0
80-81	37.260473371522	38.0	38.0	38.0	37.0	38.0
82-83	37.26747135465385	38.0	38.0	38.0	37.0	38.0
84-85	37.26347048314858	38.0	38.0	38.0	37.0	38.0
86-87	37.24249762109171	38.0	38.0	38.0	37.0	38.0
88-89	37.21044375685475	38.0	38.0	38.0	37.0	38.0
90-91	37.21890491645358	38.0	38.0	38.0	36.5	38.0
92-93	37.206238973666544	38.0	38.0	38.0	36.0	38.0
94-95	37.194150487526386	38.0	38.0	38.0	36.0	38.0
96-97	37.189473331031564	38.0	38.0	38.0	36.0	38.0
98-99	37.22761491313502	38.0	38.0	38.0	36.0	38.0
100	35.722851493689575	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	6.0
20	37.0
21	93.0
22	290.0
23	680.0
24	1428.0
25	2851.0
26	5258.0
27	8656.0
28	13729.0
29	20918.0
30	29139.0
31	38512.0
32	48743.0
33	63688.0
34	91810.0
35	170315.0
36	603418.0
37	3817770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47919456535996	19.9335820447823	13.758684818738567	22.82853857111917
2	14.230545141738071	21.249739442727734	42.40932861127295	22.110386804261243
3	18.1685035318856	27.293407316346524	30.72853338539395	23.809555766373926
4	12.034726056438993	18.268598196791157	40.2415124421817	29.455163304588144
5	12.59555056609376	38.63027071219822	35.62902809216133	13.145150629546698
6	28.47326289827657	38.64674479693607	19.501588561139624	13.378403743647738
7	25.402630891263478	33.6977779675821	23.976007377965143	16.92358376318928
8	23.923173989215726	36.83001701121235	21.86141098499292	17.385398014579007
9	25.830895703046195	16.85704721898651	22.027623042032445	35.28443403593485
10-11	23.80293634064724	27.164374271075143	29.287908413991698	19.74478097428592
12-13	24.858882181339727	25.030509350065937	29.516863266661176	20.593745201933157
14-15	22.007414570260984	26.137153280886334	27.543867676561234	24.31156447229145
16-17	20.92221513845378	29.248415557582398	28.200716036804412	21.62865326715941
18-19	20.96900868253092	28.229471391574112	30.381923578679142	20.419596347215823
20-21	22.05359018530769	27.30738796501563	30.127452549488222	20.51156930018846
22-23	21.850620591490127	27.175064852448795	29.990582908889717	20.983731647171357
24-25	21.48518703751123	27.32981053913558	30.11844634484509	21.0665560785081
26-27	21.313177430924917	27.5351702908364	30.069402327541518	21.08224995069716
28-29	21.077775211129442	27.674477531594366	30.11274144161288	21.135005815663316
30-31	21.56037456915496	27.38132853977881	30.0214189204065	21.036877970659727
32-33	21.26905899762635	27.547685205544177	29.944028496071358	21.239227300758117
34-35	21.413336121655195	27.591002896102278	30.011193687984317	20.98446729425821
36-37	21.433857637786875	27.49696908502901	29.963546342589144	21.105626934594966
38-39	21.28647127030231	27.580059280267104	30.017249320940298	21.116220128490287
40-41	21.41116832964617	27.420181828829467	29.96966359686394	21.198986244660425
42-43	21.458412154296177	27.502416880735268	29.643631214792492	21.39553975017606
44-45	21.420843331563642	27.70130801326894	29.566134629028674	21.311714026138752
46-47	21.52566265278217	27.684402873012136	29.297153515551177	21.49278095865451
48-49	21.525827440230387	27.77085871401831	29.209922161912715	21.493391683838592
50-51	21.48069768395905	27.850707338554365	29.047163989175495	21.62143098831109
52-53	21.587612103236093	27.859183903371004	28.94221325029373	21.610990743099173
54-55	21.58105613118936	27.834616538872414	28.895745313533833	21.68858201640439
56-57	21.632388211525726	27.850116421110133	28.85602214862103	21.661473218743115
58-59	21.512477501832056	27.91501942089132	28.92503950046589	21.647463576810736
60-61	21.631015810906344	27.93450590457337	28.933199505761426	21.501278778758863
62-63	21.604876430150632	27.876750905106107	28.856104395418708	21.662268269324557
64-65	21.540907383946823	27.869639358059977	28.841406777787032	21.748046480206167
66-67	21.627964272967322	27.915787795825857	28.73828382972649	21.717964101480327
68-69	21.593892227716687	27.8631464577845	28.831612889588797	21.711348424910017
70-71	21.575058435626065	27.610508304016694	28.88045977857055	21.93397348178669
72-73	21.711008340279065	27.736127673571083	28.82108917137009	21.731774814779765
74-75	21.617869585199188	27.870181821213478	28.68051958743978	21.83142900614755
76-77	21.69278833795192	27.797468401225384	28.608336269873135	21.90140699094956
78-79	21.668249717286887	27.78480116089784	28.61765002807717	21.92929909373811
80-81	21.629116329743805	27.855439919687335	28.626262187161654	21.889181563407202
82-83	21.749755910345886	27.759963233242217	28.701966704586408	21.78831415182549
84-85	21.96671808447992	27.8157330125972	28.459221752906416	21.758327150016466
86-87	21.66787226451218	27.967735963732064	28.494993216121934	21.869398555633822
88-89	21.863486995500743	27.787188050883593	28.43196300692068	21.91736194669498
90-91	21.93700335323178	27.755869906042996	28.47516350432851	21.831963236396714
92-93	21.923273657289002	27.834244450942148	28.40232288724966	21.840159004519194
94-95	21.692537423355297	27.92035188738161	28.452361359422778	21.93474932984031
96-97	21.833027082089636	28.044651186166842	28.444299865527306	21.67802186621622
98-99	22.821438610748395	29.93337559510331	28.69918172621827	18.546004067930024
100	29.007751736216008	40.84864732714297	30.143600936641015	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1553.0
1	1405.0
2	1950.5
3	2881.5
4	3567.5
5	4671.0
6	5753.0
7	7314.0
8	9369.0
9	10513.5
10	10245.5
11	9654.0
12	10245.5
13	12301.5
14	16105.5
15	20267.5
16	23182.5
17	24724.5
18	24367.0
19	22526.5
20	20541.0
21	19449.5
22	20339.5
23	23947.5
24	30425.0
25	39304.0
26	50866.5
27	63848.0
28	77485.0
29	92061.5
30	105645.0
31	118167.5
32	130475.0
33	143232.5
34	155266.5
35	167027.5
36	178188.5
37	187445.0
38	193352.5
39	196008.0
40	199245.0
41	202810.0
42	204578.5
43	205684.0
44	208432.5
45	210506.5
46	209789.0
47	209559.0
48	207592.5
49	200625.0
50	192700.5
51	185167.0
52	176372.5
53	167214.5
54	156917.0
55	144795.5
56	131110.5
57	115649.0
58	101491.5
59	87939.5
60	74014.5
61	60596.0
62	47454.0
63	36955.5
64	29549.5
65	22576.5
66	15958.0
67	11560.0
68	8693.0
69	6243.5
70	4442.0
71	3286.5
72	2410.0
73	1683.5
74	1168.5
75	795.0
76	523.5
77	327.5
78	238.5
79	172.5
80	105.0
81	63.5
82	47.0
83	34.0
84	25.0
85	21.0
86	17.0
87	11.5
88	6.0
89	2.0
90	2.0
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006405895864536656
6	4.067235469547083E-5
7	0.0
8	0.0
9	2.0336177347735415E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.999873290811519E-4
26-27	0.0
28-29	7.53028599619171E-4
30-31	0.002751950776366084
32-33	1.7385600448016698E-4
34-35	3.081395999752256E-5
36-37	2.893713191063924E-4
38-39	6.256893662945102E-4
40-41	6.214279086222911E-4
42-43	2.0209326072157929E-4
44-45	0.0
46-47	0.0016456924436233176
48-49	6.624043888265186E-5
50-51	0.002325007682026584
52-53	0.0017417744700651174
54-55	0.0030318680219841325
56-57	0.0026987749067677517
58-59	0.004293280816108108
60-61	0.0032311448148025397
62-63	0.0032711797160880586
64-65	0.0051139833839374165
66-67	0.0019492386187596451
68-69	1.3758027809226682E-4
70-71	9.13196951596376E-4
72-73	0.006943106896330929
74-75	0.006421741698791658
76-77	0.004766011289505745
78-79	0.002958927277734688
80-81	5.4338715492538346E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0023998135330514678
90-91	3.0632352402750666E-4
92-93	0.0
94-95	1.6533208602799584E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	41.0
20-21	124.0
22-23	323.0
24-25	571.0
26-27	1760.0
28-29	5207.0
30-31	15732.0
32-33	19774.0
34-35	26387.0
36-37	40228.0
38-39	48634.0
40-41	46033.0
42-43	50448.0
44-45	58970.0
46-47	59573.0
48-49	57090.0
50-51	53387.0
52-53	49942.0
54-55	52805.0
56-57	54263.0
58-59	53683.0
60-61	52190.0
62-63	50756.0
64-65	52844.0
66-67	54937.0
68-69	55549.0
70-71	54659.0
72-73	50453.0
74-75	50742.0
76-77	52305.0
78-79	53684.0
80-81	53232.0
82-83	49946.0
84-85	48757.0
86-87	50089.0
88-89	51613.0
90-91	50941.0
92-93	49962.0
94-95	55062.0
96-97	138239.0
98-99	994892.0
100-101	2151518.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.80264818488769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.47620550738705	75.87819176561653
2	7.243956494206105	12.286133880895854
3	1.881091707265502	4.785646747642383
4	0.6819237417556858	2.3131575664411854
5	0.30581781064571767	1.29670801024307
6	0.15927665637220242	0.8104249352638281
7	0.08082403174800251	0.4797864350447034
8	0.046767488210144655	0.31728054793406235
9	0.031936056120234535	0.24374359184193706
>10	0.08896324126771207	1.1327609811035892
>50	0.0018135482920192043	0.10355378636521706
>100	0.0012708401906356267	0.23541416002565715
>500	1.1465740424668992E-4	0.0780777096580931
>1k	3.821913474762909E-5	0.03911988192386345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0336177347735415E-5	0.0	0.0	0.0
3	0.0	2.0336177347735415E-5	0.0	0.0	0.0
4	0.0	1.0168088673867708E-4	0.0	0.0	0.0
5	0.0	1.0168088673867708E-4	0.0	0.0	0.0
6	0.0	1.0168088673867708E-4	0.0	0.0	0.0
7	0.0	1.0168088673867708E-4	0.0	0.0	0.0
8	0.0	1.0168088673867708E-4	0.0	0.0	0.0
9	0.0	2.2369795082508956E-4	0.0	0.0	0.0
10-11	0.0	2.2369795082508956E-4	0.0	0.0	1.0168088673867707E-5
12-13	0.0	2.2369795082508956E-4	0.0	0.0	2.0336177347735415E-5
14-15	0.0	3.152107488898989E-4	0.0	0.0	5.084044336933854E-5
16-17	0.0	3.2537883756376663E-4	0.0	0.0	6.100853204320624E-5
18-19	0.0	3.4571501491150207E-4	0.0	0.0	7.117662071707396E-5
20-21	0.0	3.6605119225923744E-4	0.0	0.0	8.134470939094166E-5
22-23	0.0	3.863873696069729E-4	0.0	0.0	8.134470939094166E-5
24-25	0.0	4.270597243024437E-4	0.0	2.0336177347735415E-5	1.0168088673867708E-4
26-27	0.0	4.473959016501791E-4	0.0	6.100853204320624E-5	1.0168088673867708E-4
28-29	0.0	4.473959016501791E-4	0.0	5.89749143084327E-4	1.0168088673867708E-4
30-31	0.0	5.490767883888562E-4	0.0	0.001911600670687129	1.0168088673867708E-4
32-33	0.0	5.592448770627239E-4	0.0	0.004840010208761029	1.0168088673867708E-4
34-35	0.0	6.304214977797978E-4	0.0	0.008876741412286508	1.2201706408641248E-4
36-37	0.0	6.914300298230041E-4	0.0	0.015191124478758354	1.2201706408641248E-4
38-39	0.0	7.117662071707395E-4	0.0	0.024200051043805144	1.3218515276028017E-4
40-41	0.0	7.524385618662104E-4	0.0	0.033554692623763434	1.4235324143414789E-4
42-43	0.0	7.626066505400781E-4	0.0	0.04242126594737608	1.4235324143414789E-4
44-45	0.0	8.134470939094166E-4	0.0	0.05208095018755039	1.4235324143414789E-4
46-47	0.0	8.541194486048874E-4	0.0	0.06212702179733169	1.6268941878188332E-4
48-49	0.0	8.541194486048874E-4	0.0	0.07293570005765307	1.6268941878188332E-4
50-51	0.0	0.0010066407787129028	0.0	0.08367320169725737	1.6268941878188332E-4
52-53	0.0	0.0010168088673867706	0.0	0.09457339275564354	1.6268941878188332E-4
54-55	0.0	0.0010269769560606384	0.0	0.10541257528198653	1.7285750745575103E-4
56-57	0.0	0.0010574812220822416	0.0	0.11523494894094272	1.9319368480348644E-4
58-59	0.0	0.0010574812220822416	0.0	0.12510816304326827	2.0336177347735415E-4
60-61	0.0	0.0010676493107561092	0.0	0.13414759387433667	2.0336177347735415E-4
62-63	0.0	0.0010981535767777123	0.0	0.1425972755623207	2.0336177347735415E-4
64-65	0.0	0.0012608429955595958	0.0	0.15131132755582535	2.0336177347735415E-4
66-67	0.0	0.0012608429955595958	0.0	0.1600457157266777	2.1352986215122184E-4
68-69	0.0	0.0013015153502550665	0.0	0.1682716994638367	2.2369795082508956E-4
70-71	0.0	0.0013116834389289341	0.0	0.17450473782091758	2.5420221684669265E-4
72-73	0.0	0.001321851527602802	0.0	0.1797311353992856	3.050426602160312E-4
74-75	0.0	0.0013523557936244049	0.0	0.1840424049970055	3.050426602160312E-4
76-77	0.0	0.0013625238822982727	0.0	0.18836384268339929	3.050426602160312E-4
78-79	0.0	0.0013625238822982727	0.0	0.19088552867451847	3.050426602160312E-4
80-81	0.0	0.0013726919709721405	0.0	0.19173964812312336	3.152107488898989E-4
82-83	0.0	0.0014031962369937436	0.0	0.1923192291775338	3.2537883756376663E-4
84-85	0.0	0.0014031962369937436	0.0	0.19264460801509758	3.2537883756376663E-4
86-87	0.0	0.001423532414341479	0.0	0.19274628890183626	3.2537883756376663E-4
88	0.0	0.0015252133010801561	0.0	0.19274628890183626	3.2537883756376663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7770	0.0	51.916576	1
GTATCAA	15245	0.0	38.444275	1
ATCAACG	18450	0.0	31.500189	3
TATCAAC	18660	0.0	31.43042	2
TCAACGC	18575	0.0	31.376219	4
CAACGCA	18860	0.0	30.880411	5
AACGCAG	19450	0.0	29.982962	6
ACGCAGA	22205	0.0	26.189323	7
CGCAGAG	22495	0.0	25.9062	8
GTGGTAT	3375	0.0	23.614065	1
GCAGAGT	25165	0.0	23.14132	9
GGACCGA	1005	0.0	22.771526	6
TGGTATC	3355	0.0	22.536636	2
CCGAACC	945	0.0	22.055075	9
GTACATG	20415	0.0	21.401205	1
TACATGG	20370	0.0	21.087332	2
ACATGGG	20525	0.0	20.549746	3
GAGTACT	14725	0.0	20.273794	12-13
TGCGCGA	470	1.337794E-7	19.89678	94
ACCGAAC	1075	0.0	19.768106	8
>>END_MODULE