FastQCFastQC Report
Fri 27 May 2016
SRR2138874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4979504
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.046.4550931
GTATCAA110150.038.2033271
ATCAACG129700.031.8968943
TCAACGC130850.031.7124674
CAACGCA133500.031.0203025
TATCAAC136850.030.5051732
AACGCAG139000.029.9716366
ACGCAGA158900.026.0864937
CGCAGAG162050.025.6310358
GCAGAGT191300.021.7557499
GTACATG150800.021.7471561
TACATGG149500.021.5445422
GTGGTAT30800.021.458241
ACATGGG150000.021.0267523
GAGTACT116500.019.19036912-13
TGGTATC30300.019.1893352
CATGGGG103100.018.947434
CAGAGTA189750.018.18585610-11
AGAGTAC174600.018.17075310-11
TGCGCGA3150.001668887816.93879794
Produced by FastQC (version 0.11.5)