##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4979504
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.737304157201198	32.0	25.0	33.0	18.0	33.0
2	31.621158653552644	33.0	32.0	33.0	27.0	33.0
3	31.327362323637054	33.0	31.0	33.0	29.0	33.0
4	32.52255405357642	33.0	33.0	33.0	31.0	34.0
5	33.135619732407086	33.0	33.0	34.0	33.0	34.0
6	37.280896049084404	38.0	38.0	38.0	36.0	38.0
7	37.34683574910272	38.0	38.0	38.0	37.0	38.0
8	37.50698643880997	38.0	38.0	38.0	37.0	38.0
9	37.548606447549794	38.0	38.0	38.0	37.0	38.0
10-11	37.5446525396907	38.0	38.0	38.0	37.5	38.0
12-13	37.57447990803904	38.0	38.0	38.0	38.0	38.0
14-15	37.56867812537152	38.0	38.0	38.0	38.0	38.0
16-17	37.582100948206886	38.0	38.0	38.0	38.0	38.0
18-19	37.57491378659401	38.0	38.0	38.0	38.0	38.0
20-21	37.56818159649521	38.0	38.0	38.0	38.0	38.0
22-23	37.57362175099957	38.0	38.0	38.0	38.0	38.0
24-25	37.530399469474474	38.0	38.0	38.0	38.0	38.0
26-27	37.53001338709004	38.0	38.0	38.0	38.0	38.0
28-29	37.52215656439628	38.0	38.0	38.0	38.0	38.0
30-31	37.5179239777014	38.0	38.0	38.0	38.0	38.0
32-33	37.51273426308437	38.0	38.0	38.0	38.0	38.0
34-35	37.49482754817201	38.0	38.0	38.0	38.0	38.0
36-37	37.47146835992085	38.0	38.0	38.0	37.5	38.0
38-39	37.449936472658095	38.0	38.0	38.0	37.0	38.0
40-41	37.43523970861992	38.0	38.0	38.0	37.0	38.0
42-43	37.41792767635299	38.0	38.0	38.0	37.0	38.0
44-45	37.38820785672533	38.0	38.0	38.0	37.0	38.0
46-47	37.35628359724065	38.0	38.0	38.0	37.0	38.0
48-49	37.31704843415065	38.0	38.0	38.0	37.0	38.0
50-51	37.30079486148743	38.0	38.0	38.0	37.0	38.0
52-53	37.29204850366193	38.0	38.0	38.0	37.0	38.0
54-55	37.270130574316866	38.0	38.0	38.0	37.0	38.0
56-57	37.23790359096698	38.0	38.0	38.0	37.0	38.0
58-59	37.24255543965236	38.0	38.0	38.0	37.0	38.0
60-61	37.25712108786857	38.0	38.0	38.0	37.0	38.0
62-63	37.26312976197392	38.0	38.0	38.0	37.0	38.0
64-65	37.26999574139413	38.0	38.0	38.0	37.0	38.0
66-67	37.2769091040891	38.0	38.0	38.0	37.0	38.0
68-69	37.27751125937926	38.0	38.0	38.0	37.0	38.0
70-71	37.2485401369981	38.0	38.0	38.0	37.0	38.0
72-73	37.22913763739297	38.0	38.0	38.0	37.0	38.0
74-75	37.21566958696589	38.0	38.0	38.0	37.0	38.0
76-77	37.219016415126	38.0	38.0	38.0	37.0	38.0
78-79	37.22250942384864	38.0	38.0	38.0	37.0	38.0
80-81	37.21491559497221	38.0	38.0	38.0	37.0	38.0
82-83	37.219024533937045	38.0	38.0	38.0	37.0	38.0
84-85	37.21219301684195	38.0	38.0	38.0	37.0	38.0
86-87	37.18649228320582	38.0	38.0	38.0	36.0	38.0
88-89	37.14865544658312	38.0	38.0	38.0	36.0	38.0
90-91	37.15547088563025	38.0	38.0	38.0	36.0	38.0
92-93	37.139694318360505	38.0	38.0	38.0	36.0	38.0
94-95	37.124213384810034	38.0	38.0	38.0	36.0	38.0
96-97	37.120719887298684	38.0	38.0	38.0	36.0	38.0
98-99	37.15786435724018	38.0	38.0	38.0	36.0	38.0
100	35.61469975576229	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	13.0
20	34.0
21	107.0
22	258.0
23	759.0
24	1622.0
25	3218.0
26	5536.0
27	9117.0
28	14459.0
29	21402.0
30	29256.0
31	39060.0
32	50700.0
33	67220.0
34	98187.0
35	178691.0
36	597527.0
37	3862333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.273607170513365	19.64661540587175	13.662806576719289	23.416970846895595
2	14.150063942111505	21.106057952759954	42.38663127893862	22.357246826189918
3	18.310659053592488	26.446790684373383	30.61668391068669	24.625866351347444
4	12.10486024310855	18.107446042818722	40.218584019613196	29.569109694459527
5	12.83983886561651	38.1781162347832	35.87800685973926	13.104038039861038
6	28.86824827061019	38.62741695856332	19.44375160407607	13.060583166750412
7	25.69382412384848	33.173685571896314	23.91386772658482	17.218622577670388
8	23.49461381989327	37.37160114172037	21.894634866170378	17.239150172215982
9	25.30779690575421	17.201221568185247	22.041606177004482	35.44937534905606
10-11	23.683919861078508	27.12769728223881	29.586527008819957	19.60185584786273
12-13	24.51102559612363	24.7519030007808	29.675194557530226	21.06187684556534
14-15	21.702382405958506	26.061692088207984	27.390418804764487	24.845506701069024
16-17	21.145901278520913	29.217679110208568	28.04456026142363	21.591859349846892
18-19	21.144505557179993	28.389333556113222	29.832931151375718	20.633229735331067
20-21	22.026975803111277	27.587053823580355	29.605886177473973	20.780084195834387
22-23	21.742514859422908	27.499200695996972	29.441258385662977	21.31702605891714
24-25	21.461736136146587	27.57257666881902	29.62700678543777	21.33868040959662
26-27	21.430328962062987	27.728394992294973	29.531343149275052	21.309932896366988
28-29	21.35240498225596	27.81135005706043	29.543175991760872	21.29306896892274
30-31	21.64707394789394	27.65990471082298	29.44256314286094	21.250458198422148
32-33	21.500634828801985	27.741988387897948	29.39876649152896	21.35861029177111
34-35	21.53552335590404	27.76180751359992	29.461999588641984	21.24066954185406
36-37	21.518118843006654	27.692036132896952	29.479554432831733	21.31029059126466
38-39	21.33878515999282	27.811941086901914	29.5358954226609	21.313378330444362
40-41	21.624652684228185	27.670664069408605	29.40230488746225	21.302378358900953
42-43	21.56745644753872	27.719601843556763	29.234509904707494	21.47843180419702
44-45	21.559272262537053	27.879212102962835	29.137245071964994	21.42427056253511
46-47	21.638600165320753	27.790871158793962	28.922995411509955	21.647533264375333
48-49	21.595948338003716	27.836382355100447	28.96258699811997	21.605082308775867
50-51	21.543359667761713	27.969268045110418	28.84779775714007	21.639574529987797
52-53	21.66524689990234	27.980489833211603	28.738267775124264	21.615995491761797
54-55	21.720631553061214	27.851292469404637	28.75176719366056	21.676308783873584
56-57	21.741401636042358	27.968830208619007	28.65565457265099	21.634113582687647
58-59	21.59020516811604	28.054027227918205	28.779063603243888	21.576704000721865
60-61	21.733630787027145	28.058852523940182	28.68708384262306	21.520432846409616
62-63	21.65084684836958	28.06784136191175	28.65376894472127	21.627542844997404
64-65	21.60481763108166	28.017115219334553	28.702903598518954	21.675163551064834
66-67	21.739497068299706	28.033723227028446	28.537078320001363	21.689701384670478
68-69	21.708372309875077	28.035759556881484	28.61310608745474	21.642762045788707
70-71	21.69279999845923	27.80522143415501	28.676419429803772	21.825559137581998
72-73	21.736363935332758	27.804855767883918	28.678908603257234	21.77987169352609
74-75	21.67140669653292	27.94476048748541	28.563057914076524	21.82077490190515
76-77	21.732799014054898	27.92305174717323	28.475977053064288	21.868172185707582
78-79	21.73947504478711	27.930763039800137	28.44533754744435	21.884424367968407
80-81	21.704147626475123	27.9186493335561	28.51999841854284	21.85720462142594
82-83	21.813815198184102	27.91956380448637	28.502777070826536	21.763843926502997
84-85	21.962024911597418	27.871274196889946	28.363981432683815	21.802719458828815
86-87	21.735886243946993	27.94621492187796	28.46632417083693	21.851574663338116
88-89	21.87409182842759	27.885235185524333	28.373077884526694	21.867595101521385
90-91	21.922542049719254	27.985549693941447	28.311614198647987	21.780294057691314
92-93	21.949541102084126	27.96689707271839	28.313369590512792	21.770192234684696
94-95	21.815529846018542	28.060307230016086	28.328662938123696	21.79549998584168
96-97	21.923615441269497	28.123107909053118	28.373824079596595	21.57945257008079
98-99	22.953652505961784	30.05235632435453	28.568182403937787	18.4258087657459
100	28.936172573594586	41.09193844851228	29.971888977893137	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	857.0
1	808.5
2	1272.0
3	2000.0
4	2553.0
5	3350.5
6	4107.5
7	5228.0
8	6676.5
9	7474.0
10	7351.0
11	7271.0
12	7987.0
13	9345.5
14	11780.5
15	14546.0
16	16711.5
17	18023.0
18	17984.0
19	17014.0
20	16225.0
21	16465.0
22	18540.5
23	22968.0
24	29564.5
25	38275.5
26	49849.0
27	62628.5
28	75695.0
29	90166.0
30	103093.5
31	113995.5
32	125401.5
33	137800.5
34	150211.5
35	161914.0
36	173908.0
37	183136.0
38	189185.0
39	195916.5
40	202954.0
41	207222.5
42	210013.5
43	214978.0
44	217514.5
45	216599.0
46	213893.5
47	210797.5
48	207239.0
49	201099.0
50	194583.0
51	186756.0
52	176027.0
53	164810.5
54	153102.0
55	139695.5
56	126204.0
57	112549.5
58	99556.0
59	85963.5
60	71628.5
61	58019.0
62	45419.0
63	35025.5
64	26491.0
65	19764.0
66	14509.5
67	10789.5
68	8114.5
69	5828.5
70	4210.0
71	2994.5
72	2067.0
73	1371.5
74	888.5
75	579.5
76	384.5
77	256.5
78	167.0
79	111.0
80	72.5
81	40.5
82	22.5
83	17.0
84	17.0
85	15.5
86	12.5
87	11.5
88	9.0
89	4.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006145190364341509
6	4.0164642904192865E-5
7	0.0
8	2.0082321452096432E-5
9	6.02469643562893E-5
10-11	2.0082321452096432E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.326474397410845E-4
26-27	1.0043047514561665E-5
28-29	6.329402606869953E-4
30-31	0.002534864445106102
32-33	1.714660173013246E-4
34-35	6.073195365180225E-5
36-37	2.950362991297752E-4
38-39	5.841794369658089E-4
40-41	5.268593408493779E-4
42-43	1.1454789507829349E-4
44-45	0.0
46-47	0.001550787438876669
48-49	2.1466436689574933E-5
50-51	0.0020161788597793133
52-53	0.0014443661349976807
54-55	0.00285018657122445
56-57	0.002489038742167062
58-59	0.003699431144788968
60-61	0.002849092136790612
62-63	0.0029244287318445075
64-65	0.004583268093497739
66-67	0.0016700210175673274
68-69	1.4276011696336382E-4
70-71	6.86120138673306E-4
72-73	0.00655253083586909
74-75	0.005935460608983184
76-77	0.004856759226534941
78-79	0.0024949360987641875
80-81	3.826104567692909E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0022189973981587134
90-91	2.0303697289740662E-4
92-93	0.0
94-95	1.2492620678368737E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	25.0
20-21	97.0
22-23	232.0
24-25	401.0
26-27	1255.0
28-29	3843.0
30-31	12740.0
32-33	16332.0
34-35	22111.0
36-37	33786.0
38-39	39452.0
40-41	37877.0
42-43	42172.0
44-45	49105.0
46-47	49820.0
48-49	46619.0
50-51	43695.0
52-53	42712.0
54-55	45929.0
56-57	46687.0
58-59	45888.0
60-61	44999.0
62-63	44230.0
64-65	45940.0
66-67	48296.0
68-69	49286.0
70-71	49277.0
72-73	45316.0
74-75	44915.0
76-77	46566.0
78-79	47726.0
80-81	46675.0
82-83	44681.0
84-85	43539.0
86-87	45382.0
88-89	46300.0
90-91	46116.0
92-93	45352.0
94-95	51304.0
96-97	144491.0
98-99	1075126.0
100-101	2343209.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.73443740700965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.20861818402291	78.24193747454969
2	7.052323310653996	12.233585895238283
3	1.6691857274875106	4.34327655004318
4	0.5606138661853292	1.944981131446125
5	0.22653754780208235	0.9824303380088585
6	0.10896770307773887	0.5670751453189053
7	0.06055159801130971	0.36763361513244464
8	0.03398357247428503	0.23580368317099612
9	0.02151787363265055	0.16797065973507652
>10	0.05575173453619889	0.6774892432904503
>50	0.0010375853296989168	0.0643334296604739
>100	8.447809154417266E-4	0.13878099664172888
>500	6.651587088321375E-5	0.034701837763778255
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0082321452096432E-5	0.0	2.0082321452096432E-5	0.0
3	0.0	2.0082321452096432E-5	0.0	2.0082321452096432E-5	0.0
4	0.0	6.02469643562893E-5	0.0	2.0082321452096432E-5	0.0
5	0.0	8.032928580838573E-5	0.0	2.0082321452096432E-5	0.0
6	0.0	8.032928580838573E-5	0.0	2.0082321452096432E-5	0.0
7	0.0	8.032928580838573E-5	0.0	2.0082321452096432E-5	0.0
8	0.0	8.032928580838573E-5	0.0	2.0082321452096432E-5	0.0
9	0.0	1.6065857161677146E-4	0.0	4.0164642904192865E-5	0.0
10-11	0.0	1.6065857161677146E-4	0.0	4.0164642904192865E-5	0.0
12-13	0.0	1.6065857161677146E-4	0.0	4.0164642904192865E-5	0.0
14-15	0.0	1.8074089306886792E-4	0.0	4.0164642904192865E-5	0.0
16-17	0.0	1.8074089306886792E-4	0.0	4.0164642904192865E-5	0.0
18-19	0.0	1.8074089306886792E-4	0.0	5.0205803630241086E-5	0.0
20-21	0.0	1.8074089306886792E-4	0.0	1.0041160726048217E-4	0.0
22-23	0.0	2.0082321452096434E-4	0.0	1.204939287125786E-4	0.0
24-25	0.0	2.409878574251572E-4	0.0	1.4057625016467503E-4	0.0
26-27	0.0	2.6107017887725363E-4	0.0	1.8074089306886792E-4	0.0
28-29	0.0	2.6107017887725363E-4	0.0	5.422226792066037E-4	0.0
30-31	0.0	3.4139946468563935E-4	0.0	0.0016467503590719076	0.0
32-33	0.0	3.4139946468563935E-4	0.0	0.003775476432994129	0.0
34-35	0.0	4.4181107194612155E-4	0.0	0.00669745420427416	0.0
36-37	0.0	5.422226792066037E-4	0.0	0.011637705281489883	0.0
38-39	0.0	5.623050006587001E-4	0.0	0.018746847075532022	0.0
40-41	0.0	5.823873221107966E-4	0.0	0.025886112351752304	0.0
42-43	0.0	5.924284828368448E-4	0.0	0.032754266288369284	0.0
44-45	0.0	6.52675447193134E-4	0.0	0.04099805924445487	0.0
46-47	0.0	7.028812508233751E-4	0.0	0.04967362211176053	0.0
48-49	0.0	7.129224115494234E-4	0.0	0.05860021399721739	0.0
50-51	0.0	8.233751795359538E-4	0.0	0.06677371882822064	0.0
52-53	0.0	8.233751795359538E-4	0.0	0.07486689437341551	0.0
54-55	0.0	8.534986617140985E-4	0.0	0.08268895857900707	0.0
56-57	0.0	8.635398224401467E-4	0.0	0.08899480751496534	0.0
58-59	0.0	8.635398224401467E-4	0.0	0.09581275564795208	0.0
60-61	0.0	8.635398224401467E-4	0.0	0.10251020985222625	0.0
62-63	0.0	8.635398224401467E-4	0.0	0.10820354798389559	0.0
64-65	0.0	9.438691082485324E-4	0.0	0.11414791513371612	0.0
66-67	0.0	9.940749118787736E-4	0.0	0.12061442264129119	0.0
68-69	0.0	0.0010342395547829663	0.0	0.12612701987989164	0.0
70-71	0.0	0.0010543218762350628	0.0	0.1313685057788888	0.0
72-73	0.0	0.001064363036961111	0.0	0.13578661649835005	0.0
74-75	0.0	0.0010944865191392557	0.0	0.13926085810956274	0.0
76-77	0.0	0.0011748158049476414	0.0	0.14252423534552838	0.0
78-79	0.0	0.001204939287125786	0.0	0.14466300258017667	0.0
80-81	0.0	0.001204939287125786	0.0	0.14534580150954796	0.0
82-83	0.0	0.001204939287125786	0.0	0.14600851811746712	0.0
84-85	0.0	0.001204939287125786	0.0	0.14635995874287883	0.0
86-87	0.0	0.0012451039300299788	0.0	0.1465206173144956	0.0
88	0.0	0.0013053508943862682	0.0	0.14654069963594768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6150	0.0	46.455093	1
GTATCAA	11015	0.0	38.203327	1
ATCAACG	12970	0.0	31.896894	3
TCAACGC	13085	0.0	31.712467	4
CAACGCA	13350	0.0	31.020302	5
TATCAAC	13685	0.0	30.505173	2
AACGCAG	13900	0.0	29.971636	6
ACGCAGA	15890	0.0	26.086493	7
CGCAGAG	16205	0.0	25.631035	8
GCAGAGT	19130	0.0	21.755749	9
GTACATG	15080	0.0	21.747156	1
TACATGG	14950	0.0	21.544542	2
GTGGTAT	3080	0.0	21.45824	1
ACATGGG	15000	0.0	21.026752	3
GAGTACT	11650	0.0	19.190369	12-13
TGGTATC	3030	0.0	19.189335	2
CATGGGG	10310	0.0	18.94743	4
CAGAGTA	18975	0.0	18.185856	10-11
AGAGTAC	17460	0.0	18.170753	10-11
TGCGCGA	315	0.0016688878	16.938797	94
>>END_MODULE