FastQCFastQC Report
Fri 27 May 2016
SRR2138873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23004607
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA255600.048.045211
GTATCAA472900.038.7395741
TCAACGC548150.032.773284
ATCAACG552350.032.4543343
CAACGCA566550.031.6937855
TATCAAC583400.031.020512
AACGCAG584350.030.6822686
ACGCAGA676450.026.5174757
CGCAGAG689000.026.00348
GCAGAGT828350.021.7322949
GTACATG612900.020.2390041
TACATGG605850.019.9022462
GTGGTAT131050.019.8911861
GAGTACT498600.019.58476412-13
ACATGGG606300.019.357993
CAGAGTA806900.018.60493510-11
AGAGTAC737500.018.5014810-11
TGGTATC131750.017.9661432
GTACTTT562300.017.34352114-15
CATGGGG471650.017.206754
Produced by FastQC (version 0.11.5)