##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23004607
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.134128307429897	32.0	27.0	33.0	18.0	33.0
2	31.753515328473117	33.0	32.0	33.0	27.0	34.0
3	31.396104136880062	33.0	31.0	33.0	29.0	33.0
4	32.53181156278827	33.0	33.0	33.0	31.0	34.0
5	33.142238204721345	33.0	33.0	34.0	33.0	34.0
6	37.29148752682452	38.0	38.0	38.0	36.0	38.0
7	37.33722732146652	38.0	38.0	38.0	37.0	38.0
8	37.50008152714802	38.0	38.0	38.0	37.0	38.0
9	37.54073521012552	38.0	38.0	38.0	37.0	38.0
10-11	37.53517899697222	38.0	38.0	38.0	37.5	38.0
12-13	37.56588949769931	38.0	38.0	38.0	38.0	38.0
14-15	37.56135021128594	38.0	38.0	38.0	38.0	38.0
16-17	37.57417677250474	38.0	38.0	38.0	38.0	38.0
18-19	37.56489489257521	38.0	38.0	38.0	38.0	38.0
20-21	37.560601142219944	38.0	38.0	38.0	38.0	38.0
22-23	37.56658881969447	38.0	38.0	38.0	38.0	38.0
24-25	37.524120084149516	38.0	38.0	38.0	38.0	38.0
26-27	37.523640363332404	38.0	38.0	38.0	38.0	38.0
28-29	37.515037358584756	38.0	38.0	38.0	37.0	38.0
30-31	37.51052777161597	38.0	38.0	38.0	37.5	38.0
32-33	37.50618065308633	38.0	38.0	38.0	38.0	38.0
34-35	37.49176043690779	38.0	38.0	38.0	37.5	38.0
36-37	37.47043122893299	38.0	38.0	38.0	37.0	38.0
38-39	37.452650222575116	38.0	38.0	38.0	37.0	38.0
40-41	37.433558083362946	38.0	38.0	38.0	37.0	38.0
42-43	37.415305827894095	38.0	38.0	38.0	37.0	38.0
44-45	37.39009733310773	38.0	38.0	38.0	37.0	38.0
46-47	37.361530983007825	38.0	38.0	38.0	37.0	38.0
48-49	37.32195860576738	38.0	38.0	38.0	37.0	38.0
50-51	37.306612213646915	38.0	38.0	38.0	37.0	38.0
52-53	37.29905900076489	38.0	38.0	38.0	37.0	38.0
54-55	37.27871448815749	38.0	38.0	38.0	37.0	38.0
56-57	37.248989068475055	38.0	38.0	38.0	37.0	38.0
58-59	37.25493674335258	38.0	38.0	38.0	37.0	38.0
60-61	37.27244437783047	38.0	38.0	38.0	37.0	38.0
62-63	37.27970643647933	38.0	38.0	38.0	37.0	38.0
64-65	37.28839740422565	38.0	38.0	38.0	37.0	38.0
66-67	37.2969129154479	38.0	38.0	38.0	37.0	38.0
68-69	37.29942076866976	38.0	38.0	38.0	37.0	38.0
70-71	37.26990944886819	38.0	38.0	38.0	37.0	38.0
72-73	37.25246817718353	38.0	38.0	38.0	37.0	38.0
74-75	37.23923982187846	38.0	38.0	38.0	37.0	38.0
76-77	37.24327124626396	38.0	38.0	38.0	37.0	38.0
78-79	37.24858586904587	38.0	38.0	38.0	37.0	38.0
80-81	37.240681400912926	38.0	38.0	38.0	37.0	38.0
82-83	37.24254502730309	38.0	38.0	38.0	37.0	38.0
84-85	37.23837904547341	38.0	38.0	38.0	37.0	38.0
86-87	37.213460806746085	38.0	38.0	38.0	36.5	38.0
88-89	37.17660767725447	38.0	38.0	38.0	36.0	38.0
90-91	37.18566526475208	38.0	38.0	38.0	36.0	38.0
92-93	37.16870415648462	38.0	38.0	38.0	36.0	38.0
94-95	37.154915548732234	38.0	38.0	38.0	36.0	38.0
96-97	37.14897633846707	38.0	38.0	38.0	36.0	38.0
98-99	37.18546940185047	38.0	38.0	38.0	36.0	38.0
100	35.67099328784588	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	42.0
20	168.0
21	510.0
22	1403.0
23	3333.0
24	7461.0
25	14382.0
26	25299.0
27	41561.0
28	64662.0
29	96224.0
30	134351.0
31	179553.0
32	233987.0
33	307680.0
34	446951.0
35	818061.0
36	2648738.0
37	1.7980228E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43439555389927	19.12480400121593	13.678321042389467	23.762479402495334
2	14.53709685194796	20.58661554183473	41.66077690438267	23.21551070183464
3	18.547619613758233	25.55767633848298	30.265055169166764	25.629648878592015
4	12.607174728088161	17.578192055182686	39.0761728726772	30.738460344051955
5	13.461638113468316	37.54455043188879	35.36894348007385	13.62486797456904
6	30.24251327865093	37.528487785658136	18.73461720431865	13.494381731372288
7	26.5847566860964	32.635688925573334	23.157903522268846	17.62165086606142
8	24.246370487718853	36.72341095518002	21.241815660742027	17.788402896359106
9	25.601856671740286	17.048864781638283	21.422949883295107	35.92632866332632
10-11	24.28499958399628	26.61806972361443	28.954621775243055	20.142308917146227
12-13	25.07292995702991	24.161695090031316	29.04087863791805	21.724496315020726
14-15	22.261214894912136	25.483895464938826	26.67924081467682	25.575648825472218
16-17	21.896577063889854	28.569399164263054	27.35405564633206	22.179968125515032
18-19	21.929661741232962	27.750361047245885	29.02179985078641	21.298177360734744
20-21	22.67307555962473	26.98732402760186	28.875770184550436	21.46383022822297
22-23	22.454881503964575	26.82047366419437	28.75886541548279	21.96577941635826
24-25	22.194629549457126	26.91656533521696	28.85420393845734	22.034601176868577
26-27	22.140355419414018	27.10926869320318	28.783700927444304	21.966674959938498
28-29	22.11562082187682	27.139133289067495	28.811901458456852	21.933344430598837
30-31	22.360281109278606	26.966145473122282	28.703856083778454	21.969717333820654
32-33	22.148309520288336	27.146718630146864	28.664843731274548	22.040128118290255
34-35	22.280365878855847	27.12857319320982	28.650861352361574	21.940199575572763
36-37	22.293600758636735	27.03159230299811	28.64379634467045	22.03101059369471
38-39	22.103539413080576	27.189009755147374	28.73879546119601	21.968655370576045
40-41	22.396316225689542	27.05380807362076	28.572597420059175	21.977278280630518
42-43	22.285630969731	27.11074607228162	28.443967185302704	22.159655772684676
44-45	22.35497152525874	27.17089369737598	28.35309579656223	22.121038980803046
46-47	22.360095784776103	27.167724878198673	28.17713627455663	22.295043062468594
48-49	22.27276229616986	27.262879504752956	28.214498487422212	22.249859711654974
50-51	22.25468052952188	27.40821400682944	28.104337867951607	22.232767595697073
52-53	22.471733080719655	27.286865227599836	27.946329448045343	22.295072243635165
54-55	22.456124239143612	27.205559180065713	27.97546929653277	22.36284728425791
56-57	22.413751555529068	27.30963244964924	27.981004991264253	22.295611003557433
58-59	22.382294670598128	27.30678258305278	28.033527855185618	22.277394891163475
60-61	22.421595122644753	27.2596023081413	28.057551160219536	22.261251408994408
62-63	22.39796923263474	27.32624251206792	28.01065609479766	22.26513216049968
64-65	22.38433700047308	27.27510965187831	27.978230896833665	22.36232245081494
66-67	22.44677539747916	27.354844774003674	27.828284167431654	22.37009566108551
68-69	22.385016927971392	27.306928960277578	27.910257980111915	22.39779613163912
70-71	22.443337747469666	27.097734508530536	27.914951502001117	22.543976241998685
72-73	22.489104636772172	27.146395294157454	27.819855459297187	22.544644609773183
74-75	22.34848926317804	27.2945853481296	27.77342341872614	22.583501969966218
76-77	22.514595638900026	27.25486774547614	27.669545747259615	22.560990868364218
78-79	22.50719435414246	27.28605905532353	27.62469964208034	22.582046948453666
80-81	22.50415240930503	27.28257416623291	27.609176433230694	22.60409699123137
82-83	22.57307531722307	27.286796434577305	27.62085388895243	22.519274359247195
84-85	22.67262359974474	27.27149628713236	27.559620073170976	22.49626003995192
86-87	22.510879051523904	27.27418622684815	27.64557414054078	22.56936058108717
88-89	22.613486531710926	27.29347109811777	27.58677613269957	22.50626623747173
90-91	22.651208773245926	27.304809644660228	27.569614325736108	22.47436725635774
92-93	22.68273848769021	27.30064929718078	27.55337681726052	22.463235397868488
94-95	22.584640889530363	27.399788453738736	27.536894971238564	22.478675685492338
96-97	22.692115429250244	27.44476465929473	27.63214943329085	22.23097047816418
98-99	23.742910638884307	29.391850148662336	27.81191546813299	19.053323744320362
100	22.652374903780434	30.22456645402461	21.736319536465277	25.386739105729678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3599.0
1	3447.0
2	5248.5
3	7949.5
4	9899.0
5	12881.0
6	15815.0
7	20229.0
8	26713.5
9	30930.5
10	31307.0
11	31556.0
12	34715.5
13	40784.5
14	51271.5
15	62672.0
16	70561.0
17	74844.0
18	74057.0
19	69064.5
20	64577.0
21	63795.5
22	69269.0
23	82796.0
24	104914.5
25	137504.5
26	179724.5
27	225618.0
28	272260.5
29	322980.0
30	370727.0
31	416556.0
32	468937.0
33	520463.5
34	572661.0
35	623228.0
36	677035.5
37	726205.0
38	759210.0
39	789302.5
40	825921.0
41	859742.5
42	883414.5
43	907438.0
44	927472.0
45	946409.5
46	958552.0
47	961816.5
48	963887.5
49	953665.0
50	934431.5
51	908939.5
52	875839.5
53	837219.0
54	794848.0
55	748905.0
56	696896.5
57	637067.0
58	573276.5
59	504984.5
60	425623.5
61	344643.0
62	273571.0
63	213747.5
64	160974.5
65	118288.0
66	87992.5
67	67046.5
68	50755.0
69	36999.5
70	26901.5
71	19507.5
72	13904.0
73	9557.5
74	6137.5
75	3914.0
76	2523.5
77	1522.5
78	981.0
79	633.0
80	406.5
81	263.5
82	176.5
83	121.5
84	75.0
85	45.5
86	36.0
87	32.0
88	23.0
89	13.5
90	10.0
91	6.5
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006359595710546152
6	4.781650910185077E-5
7	1.3040866118686574E-5
8	4.346955372895525E-6
9	8.69391074579105E-6
10-11	2.1734776864477626E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.17320835832414E-4
26-27	0.0
28-29	6.762690199030974E-4
30-31	0.0024834050430142733
32-33	1.810180858440978E-4
34-35	4.1546611537082934E-5
36-37	3.3804879203093435E-4
38-39	6.15884917024739E-4
40-41	6.240800510368667E-4
42-43	1.4750125498984456E-4
44-45	0.0
46-47	0.0014959904618009052
48-49	5.951692583540933E-5
50-51	0.0020673457728328016
52-53	0.0015595202283602448
54-55	0.0028519666347065326
56-57	0.0026126895238372163
58-59	0.0038335579093902537
60-61	0.00287459589792286
62-63	0.0030289399486768866
64-65	0.004371976974319661
66-67	0.0016937894133779476
68-69	1.0935129259316724E-4
70-71	7.527318331872992E-4
72-73	0.006987599328572495
74-75	0.006189946086131848
76-77	0.004854959002683074
78-79	0.0028002187452738168
80-81	3.946217787024204E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0022491540020644608
90-91	2.626667079928954E-4
92-93	0.0
94-95	1.1863343435346882E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	477.0
22-23	1104.0
24-25	1630.0
26-27	4824.0
28-29	13569.0
30-31	44339.0
32-33	55619.0
34-35	77044.0
36-37	121370.0
38-39	137205.0
40-41	137976.0
42-43	155117.0
44-45	181771.0
46-47	185398.0
48-49	175649.0
50-51	160043.0
52-53	156337.0
54-55	168899.0
56-57	170473.0
58-59	174199.0
60-61	171125.0
62-63	171788.0
64-65	181456.0
66-67	190456.0
68-69	191645.0
70-71	188123.0
72-73	178563.0
74-75	179770.0
76-77	186250.0
78-79	189095.0
80-81	188638.0
82-83	179904.0
84-85	179236.0
86-87	184899.0
88-89	188753.0
90-91	190267.0
92-93	188901.0
94-95	221661.0
96-97	705134.0
98-99	1523536.0
100-101	1.5302241E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.09590109133731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.52038817011872	70.10024354349277
2	7.997714104234525	12.811682356991236
3	2.245882110190631	5.3965785408189815
4	0.9087720228816952	2.911556562372271
5	0.48481967525125624	1.9416034378029448
6	0.25499061603436707	1.22542218966647
7	0.16079278458433643	0.9015190079187403
8	0.10870663576828643	0.6965564757174959
9	0.07206647740025199	0.5195006501266509
>10	0.2393970552658505	2.913455800142657
>50	0.004683752866832046	0.25178820375534383
>100	0.0016911671660947516	0.213552854503288
>500	3.589421041916678E-5	0.015974239336890163
>1k	5.953402662219831E-5	0.10056613735433056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.346955372895525E-6
2	0.0	2.1734776864477623E-5	0.0	8.69391074579105E-6	4.346955372895525E-6
3	0.0	2.608173223737315E-5	0.0	1.3040866118686574E-5	4.346955372895525E-6
4	0.0	7.389824133922392E-5	0.0	1.73878214915821E-5	4.346955372895525E-6
5	0.0	8.259215208501498E-5	0.0	2.608173223737315E-5	4.346955372895525E-6
6	5.651041984764182E-5	8.693910745791049E-5	0.0	2.608173223737315E-5	1.3040866118686574E-5
7	5.651041984764182E-5	8.693910745791049E-5	0.0	3.0428687610268675E-5	1.3040866118686574E-5
8	5.651041984764182E-5	1.043269289494926E-4	0.0	3.0428687610268675E-5	1.3040866118686574E-5
9	5.651041984764182E-5	2.4777645625504493E-4	0.0	5.21634644747463E-5	1.73878214915821E-5
10-11	5.651041984764182E-5	2.4777645625504493E-4	0.0	7.389824133922392E-5	1.73878214915821E-5
12-13	5.8683897534089585E-5	2.499499339414927E-4	0.0	8.476562977146273E-5	1.73878214915821E-5
14-15	6.085737522053735E-5	3.93399461247045E-4	0.0	9.780649589014932E-5	3.47756429831642E-5
16-17	9.128606283080602E-5	4.042668496792838E-4	0.0	1.1084736200883588E-4	4.3469553728955246E-5
18-19	9.128606283080602E-5	4.39042492662448E-4	0.0	1.1736779506817917E-4	4.7816509101850775E-5
20-21	9.128606283080602E-5	4.868590017642988E-4	0.0	1.217147504410747E-4	4.7816509101850775E-5
22-23	9.997997357659707E-5	5.498898546712839E-4	0.0	1.6083734879713442E-4	5.433694216119406E-5
24-25	1.043269289494926E-4	6.346554844427466E-4	0.0	1.9126603640740307E-4	6.085737522053735E-5
26-27	1.043269289494926E-4	6.563902613072242E-4	0.0	2.303886347634628E-4	6.085737522053735E-5
28-29	1.043269289494926E-4	6.563902613072242E-4	0.0	5.12940734001672E-4	6.303085290698511E-5
30-31	1.043269289494926E-4	7.976663109263288E-4	0.0	0.0017366086714717623	6.520433059343287E-5
32-33	1.0650040663594036E-4	8.020132662992243E-4	0.0	0.004020933719928361	6.520433059343287E-5
34-35	1.1736779506817917E-4	9.23728016740299E-4	0.0	0.007055108570209437	7.389824133922392E-5
36-37	1.217147504410747E-4	0.0010584836333000602	0.0	0.011593329979512365	7.389824133922392E-5
38-39	1.217147504410747E-4	0.001088912320910329	0.0	0.02189561421327476	7.607171902567168E-5
40-41	1.217147504410747E-4	0.0012301883705294334	0.0	0.032652155283504736	8.259215208501498E-5
42-43	1.217147504410747E-4	0.0012562701027668067	0.0	0.040111530703393454	8.476562977146273E-5
44-45	1.217147504410747E-4	0.0013584235540298516	0.0	0.04953355647414451	8.911258514435826E-5
46-47	1.2388822812752246E-4	0.0014431891838013143	0.0	0.059548941653295794	9.563301820370154E-5
48-49	1.2606170581397022E-4	0.0014518830945471053	0.0	0.06888620179427538	1.1302083969528364E-4
50-51	1.4344952730555233E-4	0.001610546965657792	0.0	0.07860164705269687	1.1302083969528364E-4
52-53	1.5214343805134336E-4	0.0016214143540900308	0.0	0.10001909617495314	1.151943173817314E-4
54-55	1.5214343805134336E-4	0.001636628697895165	0.0	0.1263703396454458	1.1736779506817917E-4
56-57	1.5214343805134336E-4	0.0016779247739376725	0.0	0.16619279781654173	1.1954127275462694E-4
58-59	1.5214343805134336E-4	0.0016800982516241203	0.0	0.1961541877242241	1.2606170581397022E-4
60-61	1.6301082648358217E-4	0.001721394327666628	0.0	0.20921026818671581	1.3040866118686574E-4
62-63	1.8691908103450757E-4	0.001773557792141374	0.0	0.22037759653968442	1.3475561655976127E-4
64-65	1.8691908103450757E-4	0.002012640337650628	0.0	0.22570696382685435	1.3475561655976127E-4
66-67	1.8909255872095532E-4	0.002038722069888001	0.0	0.23109284153387188	1.3910257193265678E-4
68-69	1.912660364074031E-4	0.0020930590120491954	0.0	0.23627223886067691	1.434495273055523E-4
70-71	2.086538578989852E-4	0.002132181610405255	0.0	0.24099303239564146	1.434495273055523E-4
72-73	2.434295008821494E-4	0.0021495694318968374	0.0	0.24493572091885768	1.6953125954292545E-4
74-75	2.434295008821494E-4	0.0021952124633122403	0.0	0.24779384407653648	1.6953125954292545E-4
76-77	2.456029785685972E-4	0.0022908454815159414	0.0	0.25065414071190173	1.6953125954292545E-4
78-79	2.4777645625504493E-4	0.0023299680798720013	0.0	0.25248855587926367	1.6953125954292545E-4
80-81	2.4777645625504493E-4	0.002334315035244897	0.0	0.2531536400513167	1.717047372293732E-4
82-83	2.4777645625504493E-4	0.0023451824236771354	0.0	0.25364919296382676	1.8691908103450757E-4
84-85	2.4777645625504493E-4	0.0023625702451687174	0.0	0.2538817550762767	1.8691908103450757E-4
86-87	2.4777645625504493E-4	0.002416907187329912	0.0	0.25395130636224306	1.8909255872095532E-4
88	2.4777645625504493E-4	0.0025516628038896733	0.0	0.2539578267953024	1.956129917802986E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25560	0.0	48.04521	1
GTATCAA	47290	0.0	38.739574	1
TCAACGC	54815	0.0	32.77328	4
ATCAACG	55235	0.0	32.454334	3
CAACGCA	56655	0.0	31.693785	5
TATCAAC	58340	0.0	31.02051	2
AACGCAG	58435	0.0	30.682268	6
ACGCAGA	67645	0.0	26.517475	7
CGCAGAG	68900	0.0	26.0034	8
GCAGAGT	82835	0.0	21.732294	9
GTACATG	61290	0.0	20.239004	1
TACATGG	60585	0.0	19.902246	2
GTGGTAT	13105	0.0	19.891186	1
GAGTACT	49860	0.0	19.584764	12-13
ACATGGG	60630	0.0	19.35799	3
CAGAGTA	80690	0.0	18.604935	10-11
AGAGTAC	73750	0.0	18.50148	10-11
TGGTATC	13175	0.0	17.966143	2
GTACTTT	56230	0.0	17.343521	14-15
CATGGGG	47165	0.0	17.20675	4
>>END_MODULE