FastQCFastQC Report
Fri 27 May 2016
SRR2138872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8120883
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.050.4879151
GTATCAA198350.037.273681
TCAACGC231350.031.2146034
ATCAACG231350.031.1774863
TATCAAC241400.030.2707792
CAACGCA238650.030.2238065
AACGCAG244800.029.4628756
ACGCAGA280550.025.7696847
CGCAGAG285200.025.3645768
GTGGTAT49550.025.0412221
TGGTATC50600.022.7397442
GCAGAGT334400.021.7225769
TACATGG244450.020.1629092
GTACATG250450.020.074151
GAGTACT202950.020.00090812-13
CAGAGTA327000.019.58180610-11
ACATGGG246300.019.244473
AGAGTAC303850.018.13484610-11
GTACTTT224150.018.1189214-15
CATGGGG184200.017.4346524
Produced by FastQC (version 0.11.5)