##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8120883
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.780906953098572	32.0	25.0	33.0	18.0	33.0
2	31.635961015569364	33.0	32.0	33.0	27.0	33.0
3	31.323440936164207	33.0	31.0	33.0	29.0	33.0
4	32.532542828162896	33.0	33.0	33.0	31.0	34.0
5	33.14575619424637	33.0	33.0	34.0	33.0	34.0
6	37.2966826390677	38.0	38.0	38.0	36.0	38.0
7	37.3399950473366	38.0	38.0	38.0	37.0	38.0
8	37.49621266554388	38.0	38.0	38.0	37.0	38.0
9	37.537155380763394	38.0	38.0	38.0	37.0	38.0
10-11	37.53138020828523	38.0	38.0	38.0	37.5	38.0
12-13	37.561163053328066	38.0	38.0	38.0	37.5	38.0
14-15	37.55566814593931	38.0	38.0	38.0	38.0	38.0
16-17	37.567013032942356	38.0	38.0	38.0	38.0	38.0
18-19	37.56063847983033	38.0	38.0	38.0	38.0	38.0
20-21	37.5561197431266	38.0	38.0	38.0	38.0	38.0
22-23	37.56059509460803	38.0	38.0	38.0	38.0	38.0
24-25	37.51797877060342	38.0	38.0	38.0	38.0	38.0
26-27	37.51791216792369	38.0	38.0	38.0	37.0	38.0
28-29	37.51098069712326	38.0	38.0	38.0	37.0	38.0
30-31	37.50363415390949	38.0	38.0	38.0	37.5	38.0
32-33	37.49793536194373	38.0	38.0	38.0	37.0	38.0
34-35	37.4816842745135	38.0	38.0	38.0	37.0	38.0
36-37	37.457652426139184	38.0	38.0	38.0	37.0	38.0
38-39	37.435625212029365	38.0	38.0	38.0	37.0	38.0
40-41	37.418466828751406	38.0	38.0	38.0	37.0	38.0
42-43	37.39781620871561	38.0	38.0	38.0	37.0	38.0
44-45	37.367566651297906	38.0	38.0	38.0	37.0	38.0
46-47	37.33089092145819	38.0	38.0	38.0	37.0	38.0
48-49	37.28819125901301	38.0	38.0	38.0	37.0	38.0
50-51	37.27099549495304	38.0	38.0	38.0	37.0	38.0
52-53	37.26040677440905	38.0	38.0	38.0	37.0	38.0
54-55	37.240149745319144	38.0	38.0	38.0	37.0	38.0
56-57	37.208799933420224	38.0	38.0	38.0	37.0	38.0
58-59	37.21330089989422	38.0	38.0	38.0	37.0	38.0
60-61	37.23039951195183	38.0	38.0	38.0	37.0	38.0
62-63	37.2376725123262	38.0	38.0	38.0	37.0	38.0
64-65	37.24662708531042	38.0	38.0	38.0	37.0	38.0
66-67	37.2545821950939	38.0	38.0	38.0	37.0	38.0
68-69	37.255207147958174	38.0	38.0	38.0	37.0	38.0
70-71	37.223034917070734	38.0	38.0	38.0	37.0	38.0
72-73	37.206099964047496	38.0	38.0	38.0	37.0	38.0
74-75	37.192541563518105	38.0	38.0	38.0	36.5	38.0
76-77	37.196192268164324	38.0	38.0	38.0	36.5	38.0
78-79	37.201720525403225	38.0	38.0	38.0	36.5	38.0
80-81	37.19382881206543	38.0	38.0	38.0	36.5	38.0
82-83	37.194887308707976	38.0	38.0	38.0	36.0	38.0
84-85	37.19062993514289	38.0	38.0	38.0	36.0	38.0
86-87	37.165478355726066	38.0	38.0	38.0	36.0	38.0
88-89	37.12567670956427	38.0	38.0	38.0	36.0	38.0
90-91	37.13330332233799	38.0	38.0	38.0	36.0	38.0
92-93	37.115912839785736	38.0	38.0	38.0	36.0	38.0
94-95	37.10230156010981	38.0	38.0	38.0	36.0	38.0
96-97	37.096375154637144	38.0	38.0	38.0	36.0	38.0
98-99	37.134273402484624	38.0	38.0	38.0	36.0	38.0
100	35.56416753230868	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	21.0
20	73.0
21	206.0
22	538.0
23	1297.0
24	2641.0
25	5313.0
26	9392.0
27	15406.0
28	24078.0
29	35883.0
30	50394.0
31	66908.0
32	86966.0
33	114763.0
34	165485.0
35	300641.0
36	965161.0
37	6275712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38446939821692	19.2614276058404	14.02558071579162	23.328522280151063
2	14.162277673499299	20.637546434297846	42.49039174680881	22.70978414539404
3	18.51790008549563	25.951833070369318	30.335962234648623	25.19430460948643
4	12.297837562737945	17.916352199631493	39.62441030119508	30.161399936435483
5	13.160769230958685	37.83058318890324	35.580281418872076	13.428366161266004
6	29.560093295296408	37.99043478164499	19.088729412486156	13.360742510572438
7	26.22462360312296	32.93826545709377	23.302736906811734	17.534374032971538
8	23.908102111556097	37.27507218119015	21.3578498791326	17.458975828121154
9	25.447355909449747	17.407836069058007	21.551080098063228	35.593727923429014
10-11	24.004472175153914	27.011947780785594	29.04768762451313	19.935892419547365
12-13	24.89141267027243	24.46019724702351	29.276699344147673	21.37169073855639
14-15	22.023300914444896	25.81032752226574	27.006847654374532	25.15952390891483
16-17	21.45095551801448	28.99408229376042	27.69556586395839	21.859396324266708
18-19	21.506805356018553	28.064318867788145	29.54391166576344	20.884964110429863
20-21	22.30654771341896	27.220612942239608	29.416066667967854	21.056772676373573
22-23	22.01860581559654	27.153445919232688	29.292322276433957	21.53562598873681
24-25	21.8155838573991	27.232082985170575	29.395887071498112	21.556446085932212
26-27	21.740113058367015	27.42093390280449	29.317305851973792	21.521647186854707
28-29	21.60504941870368	27.540566863938203	29.35215171516465	21.502232002193473
30-31	21.992782772374316	27.271695336955226	29.291282206597735	21.444239684072723
32-33	21.73779176561712	27.451114120036863	29.23728797853441	21.57380613581161
34-35	21.839433610727895	27.419341253772544	29.2925968030056	21.448628332493957
36-37	21.7683640642258	27.438336807000844	29.27689406880597	21.516405059967386
38-39	21.64568260446975	27.4954511786932	29.354139255499685	21.50472696133736
40-41	21.88268647298974	27.358883941178924	29.2017918405567	21.55663774527464
42-43	21.848545716228827	27.39828237936325	29.058623689246684	21.694548215161245
44-45	21.83155722232267	27.57874385203369	28.954795079257018	21.634903846386624
46-47	21.892106520012774	27.493141013668133	28.745459992051053	21.869292474268043
48-49	21.79476652807579	27.618283583249326	28.780385177648775	21.80656471102611
50-51	21.78617869317763	27.74216548851144	28.672679933023893	21.79897588528704
52-53	21.99150219208164	27.673727711295363	28.55656751023876	21.77820258638424
54-55	21.912799692151978	27.578965148284233	28.596216471589912	21.912018687973873
56-57	21.939249801604937	27.677133066157094	28.52598586892369	21.85763126331427
58-59	21.887528662144703	27.739693758786487	28.59741755403551	21.775360025033297
60-61	21.960956958779107	27.759709760868706	28.57481998435362	21.704513295998563
62-63	21.89051532695689	27.79293555321199	28.574011893289956	21.742537226541163
64-65	21.86599438262542	27.775176695199637	28.551880376177813	21.806948545997134
66-67	21.95412244148618	27.802501206622605	28.37544471099349	21.867931640897726
68-69	21.887250390116993	27.804142173915693	28.438840397600153	21.869767038367165
70-71	21.9352383485404	27.576902427447834	28.461534108552904	22.02632511545886
72-73	21.97076986914003	27.59111383453352	28.416689342014955	22.021426954311494
74-75	21.87295034759944	27.716461842797475	28.362243944950873	22.04834386465222
76-77	21.99317579461486	27.677324803355173	28.241873628297004	22.087625773732963
78-79	21.973576304716342	27.74276786543286	28.190946570145798	22.092709259705
80-81	22.022690314244432	27.730733134338614	28.130028113754534	22.11654843766242
82-83	22.03404854312406	27.725594599121354	28.229028967861264	22.011327889893323
84-85	22.16262978518592	27.699286386516015	28.154703964245343	21.983379864052722
86-87	21.96007824416966	27.783574995106697	28.201142065342893	22.055204695380752
88-89	22.099004652127647	27.793470118435256	28.105888725409223	22.001636504027875
90-91	22.14797703130101	27.777647150939416	28.12777881115247	21.946597006607107
92-93	22.168784962223086	27.74628046780795	28.0944369581071	21.99049761186187
94-95	22.057851359450005	27.889809860979298	28.061632486807593	21.990706292763104
96-97	22.140348838321405	27.953849355063255	28.16340728299394	21.7423945236214
98-99	23.128886749444785	29.855460161416552	28.42119508689138	18.594458002247283
100	22.054757258157267	30.774440770917348	22.392896197808177	24.777905773117208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1435.0
1	1373.0
2	2079.5
3	3173.0
4	3932.5
5	5038.5
6	6224.0
7	8244.0
8	11264.0
9	13249.0
10	13310.5
11	13183.5
12	14183.5
13	16355.0
14	20537.5
15	25046.0
16	28089.5
17	29747.0
18	29416.5
19	27719.5
20	26038.0
21	25524.5
22	27493.0
23	32633.5
24	41366.0
25	53370.0
26	68816.0
27	87096.5
28	105246.5
29	124065.0
30	142963.0
31	161359.5
32	180520.5
33	199234.5
34	218610.5
35	236758.5
36	255148.0
37	272393.0
38	283470.5
39	293053.0
40	305329.0
41	315294.0
42	320935.0
43	328307.0
44	333499.5
45	336860.0
46	338268.0
47	336005.5
48	332404.0
49	325496.5
50	316844.0
51	305122.0
52	289675.5
53	273579.0
54	257127.5
55	238518.0
56	219484.0
57	198730.5
58	176785.0
59	155918.5
60	132032.0
61	106472.5
62	83726.0
63	64648.5
64	48935.5
65	36139.5
66	26474.5
67	20072.5
68	15222.5
69	11011.0
70	7994.0
71	5730.5
72	4054.5
73	2829.5
74	1844.5
75	1174.5
76	728.0
77	419.0
78	246.5
79	147.0
80	98.0
81	71.0
82	47.0
83	31.5
84	23.5
85	16.0
86	8.0
87	4.5
88	3.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.005676722592851048
6	2.4627863743388492E-5
7	0.0
8	0.0
9	0.0
10-11	3.0784829679235615E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.773348965401734E-4
26-27	1.847439095321391E-5
28-29	8.131201619735363E-4
30-31	0.002632740202245006
32-33	1.5444092724108767E-4
34-35	7.43175420117065E-5
36-37	2.6730011406255334E-4
38-39	5.375424658548024E-4
40-41	6.602678952093226E-4
42-43	1.8346558777091777E-4
44-45	0.0
46-47	0.0015671565101222896
48-49	4.532779539913811E-5
50-51	0.0020360237865004573
52-53	0.0015050494408741326
54-55	0.002620928377837271
56-57	0.002313805349624657
58-59	0.003716414083798081
60-61	0.0028251297527218805
62-63	0.002868107417178447
64-65	0.004385047058370001
66-67	0.0017497544268266238
68-69	7.708085010367375E-5
70-71	8.558984596020525E-4
72-73	0.006612908339948623
74-75	0.006021856023905328
76-77	0.004636169344006018
78-79	0.002706160475316186
80-81	7.403811348813765E-6
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.002158909408089272
90-91	2.7159961932597356E-4
92-93	0.0
94-95	5.543939681936261E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	63.0
20-21	206.0
22-23	447.0
24-25	583.0
26-27	1727.0
28-29	4962.0
30-31	14950.0
32-33	18857.0
34-35	26039.0
36-37	41596.0
38-39	48447.0
40-41	47390.0
42-43	52337.0
44-45	62082.0
46-47	64231.0
48-49	60596.0
50-51	55778.0
52-53	52301.0
54-55	56140.0
56-57	57397.0
58-59	59329.0
60-61	59215.0
62-63	57940.0
64-65	61118.0
66-67	64846.0
68-69	66953.0
70-71	68401.0
72-73	60878.0
74-75	60199.0
76-77	62032.0
78-79	64222.0
80-81	64296.0
82-83	60843.0
84-85	59146.0
86-87	61562.0
88-89	64086.0
90-91	64727.0
92-93	64379.0
94-95	75244.0
96-97	241263.0
98-99	536445.0
100-101	5477630.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.21483516924316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.92866069280234	74.89112502065902
2	7.681999769710584	12.938766887526818
3	1.919092038564963	4.848480591070656
4	0.6970318604111265	2.3480169292893573
5	0.32303239781241894	1.3602060068049122
6	0.1615230028054609	0.8161579834381852
7	0.08982601303328008	0.529527801705558
8	0.05286648440493805	0.35617138161113776
9	0.035132846708994005	0.2662836205161795
>10	0.10836867716388467	1.374473870963645
>50	0.0017084889737472738	0.10050660036857176
>100	6.86804815957417E-4	0.109281611408551
>500	2.364094290002593E-5	0.013816971680423942
>1k	4.7281849485873385E-5	0.047184722956983435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4627863743388495E-5	0.0	0.0	0.0
3	0.0	2.4627863743388495E-5	0.0	0.0	0.0
4	0.0	6.156965935847124E-5	0.0	0.0	0.0
5	2.4627863743388495E-5	6.156965935847124E-5	0.0	0.0	0.0
6	2.4627863743388495E-5	6.156965935847124E-5	0.0	0.0	0.0
7	2.4627863743388495E-5	6.156965935847124E-5	0.0	0.0	0.0
8	2.4627863743388495E-5	7.388359123016549E-5	0.0	1.2313931871694248E-5	0.0
9	2.4627863743388495E-5	2.2165077369049646E-4	0.0	2.4627863743388495E-5	0.0
10-11	4.925572748677699E-5	2.339647055621907E-4	0.0	2.4627863743388495E-5	0.0
12-13	6.156965935847124E-5	2.339647055621907E-4	0.0	2.4627863743388495E-5	0.0
14-15	6.772662529431837E-5	3.2631919459989755E-4	0.0	4.309876155092987E-5	2.4627863743388495E-5
16-17	8.619752310185974E-5	3.3863312647159185E-4	0.0	5.541269342262412E-5	4.925572748677699E-5
18-19	9.851145497355398E-5	3.6326099021498034E-4	0.0	7.388359123016549E-5	6.156965935847124E-5
20-21	9.851145497355398E-5	4.186736836376044E-4	0.0	8.619752310185974E-5	6.772662529431837E-5
22-23	1.3545325058863673E-4	4.371445814451458E-4	0.0	8.619752310185974E-5	8.004055716601261E-5
24-25	1.8470897807541372E-4	4.8024334299607565E-4	0.0	1.046684209094011E-4	9.235448903770686E-5
26-27	1.9702290994710796E-4	4.8024334299607565E-4	0.0	1.4776718246033098E-4	9.851145497355398E-5
28-29	1.9702290994710796E-4	4.8024334299607565E-4	0.0	3.078482967923562E-4	9.851145497355398E-5
30-31	2.093368418188022E-4	5.294990704828526E-4	0.0	0.0012498640849769663	1.046684209094011E-4
32-33	2.093368418188022E-4	5.294990704828526E-4	0.0	0.003152366559153728	1.3545325058863673E-4
34-35	2.1549380775464933E-4	5.787547979696296E-4	0.0	0.005504327546647329	1.3545325058863673E-4
36-37	2.2165077369049646E-4	6.403244573281009E-4	0.0	0.009691064383023373	1.3545325058863673E-4
38-39	2.2165077369049646E-4	6.526383891997951E-4	0.0	0.019339030004495817	1.3545325058863673E-4
40-41	2.2165077369049646E-4	7.142080485582664E-4	0.0	0.02952265166238696	1.3545325058863673E-4
42-43	2.2165077369049646E-4	7.511498441733491E-4	0.0	0.03579044298507933	1.3545325058863673E-4
44-45	2.2165077369049646E-4	8.373473672752088E-4	0.0	0.04351743523456747	1.3545325058863673E-4
46-47	2.2165077369049646E-4	8.866030947619858E-4	0.0	0.05170619992924415	1.4161021652448386E-4
48-49	2.2165077369049646E-4	8.92760060697833E-4	0.0	0.059759511373332186	1.7239504620371947E-4
50-51	2.2165077369049646E-4	9.851145497355397E-4	0.0	0.0687117398440539	1.7239504620371947E-4
52-53	2.4012167149803783E-4	9.97428481607234E-4	0.0	0.08951612774128134	1.785520121395666E-4
54-55	2.46278637433885E-4	0.0010035854475430811	0.0	0.11502443761349597	1.8470897807541372E-4
56-57	2.46278637433885E-4	0.0010097424134789283	0.0	0.15458294375131376	1.8470897807541372E-4
58-59	2.46278637433885E-4	0.0010282133112864697	0.0	0.18317589355738778	1.9702290994710796E-4
60-61	2.46278637433885E-4	0.0010589981409657053	0.0	0.19397521180886365	1.9702290994710796E-4
62-63	2.46278637433885E-4	0.0010836260047090939	0.0	0.2030752074620457	1.9702290994710796E-4
64-65	2.46278637433885E-4	0.0012498640849769663	0.0	0.2075205368677273	1.9702290994710796E-4
66-67	2.46278637433885E-4	0.0012560210509128132	0.0	0.21198433717121648	1.9702290994710796E-4
68-69	2.46278637433885E-4	0.0012744919487203547	0.0	0.21658974769123013	1.9702290994710796E-4
70-71	2.46278637433885E-4	0.0012929628465278959	0.0	0.22092425171006652	1.9702290994710796E-4
72-73	2.46278637433885E-4	0.0013175907102712845	0.0	0.2244645071231786	2.2165077369049646E-4
74-75	2.46278637433885E-4	0.0013483755399505202	0.0	0.22726592662398903	2.2165077369049646E-4
76-77	2.46278637433885E-4	0.0014099451993089913	0.0	0.22995652073795425	2.3396470556219073E-4
78-79	2.585925693055792E-4	0.001440730028988227	0.0	0.23156964581314618	2.46278637433885E-4
80-81	2.585925693055792E-4	0.001440730028988227	0.0	0.2321053018495649	2.46278637433885E-4
82-83	2.7706346711312056E-4	0.0014468869949240742	0.0	0.23253013249913834	2.46278637433885E-4
84-85	2.832204330489677E-4	0.0014530439608599214	0.0	0.23277641113657221	2.46278637433885E-4
86-87	2.832204330489677E-4	0.0015022996883466983	0.0	0.2328133529321873	2.46278637433885E-4
88	2.832204330489677E-4	0.0016254390070636407	0.0	0.23281950989812314	2.7090650117727347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11140	0.0	50.487915	1
GTATCAA	19835	0.0	37.27368	1
TCAACGC	23135	0.0	31.214603	4
ATCAACG	23135	0.0	31.177486	3
TATCAAC	24140	0.0	30.270779	2
CAACGCA	23865	0.0	30.223806	5
AACGCAG	24480	0.0	29.462875	6
ACGCAGA	28055	0.0	25.769684	7
CGCAGAG	28520	0.0	25.364576	8
GTGGTAT	4955	0.0	25.041222	1
TGGTATC	5060	0.0	22.739744	2
GCAGAGT	33440	0.0	21.722576	9
TACATGG	24445	0.0	20.162909	2
GTACATG	25045	0.0	20.07415	1
GAGTACT	20295	0.0	20.000908	12-13
CAGAGTA	32700	0.0	19.581806	10-11
ACATGGG	24630	0.0	19.24447	3
AGAGTAC	30385	0.0	18.134846	10-11
GTACTTT	22415	0.0	18.11892	14-15
CATGGGG	18420	0.0	17.434652	4
>>END_MODULE