FastQCFastQC Report
Fri 27 May 2016
SRR2138871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6673129
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89250.051.745411
GTATCAA162900.037.3266941
TCAACGC190900.031.4924534
ATCAACG191600.031.2654933
CAACGCA195700.030.7419365
TATCAAC198150.030.275272
AACGCAG202700.029.6996736
CGAGACC43400.029.40046386-87
ACCGAGG45900.028.56597990-91
AGACCGA45750.028.12241788-89
GAGACCG45000.027.85329288-89
ACGAGAC45100.027.68511486-87
GACCGAG46300.027.5308690-91
ATCTCCG42850.027.37763274-75
CCCACGA46500.026.89581782-83
TCTCCGA45200.026.01140674-75
ACGCAGA232850.025.8540887
GCCCACG48500.025.62069982-83
TCCGAGC46700.025.58230276-77
CGCAGAG237750.025.3032028
Produced by FastQC (version 0.11.5)