##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6673129
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.082714270921482	32.0	25.0	33.0	18.0	34.0
2	31.72623322582255	33.0	32.0	33.0	27.0	34.0
3	31.387129336177974	33.0	31.0	33.0	29.0	33.0
4	32.542814173081325	33.0	33.0	33.0	31.0	34.0
5	33.14327236892918	33.0	33.0	34.0	33.0	34.0
6	37.28750530673092	38.0	38.0	38.0	36.0	38.0
7	37.3407879571937	38.0	38.0	38.0	37.0	38.0
8	37.49635710623907	38.0	38.0	38.0	37.0	38.0
9	37.53881335127794	38.0	38.0	38.0	37.0	38.0
10-11	37.53405763623032	38.0	38.0	38.0	37.5	38.0
12-13	37.56263291178696	38.0	38.0	38.0	38.0	38.0
14-15	37.55779934720279	38.0	38.0	38.0	38.0	38.0
16-17	37.56967263782852	38.0	38.0	38.0	38.0	38.0
18-19	37.56212355553144	38.0	38.0	38.0	38.0	38.0
20-21	37.557342899571324	38.0	38.0	38.0	38.0	38.0
22-23	37.56166085012803	38.0	38.0	38.0	38.0	38.0
24-25	37.51836613373787	38.0	38.0	38.0	38.0	38.0
26-27	37.51899331190667	38.0	38.0	38.0	38.0	38.0
28-29	37.51145122581641	38.0	38.0	38.0	37.5	38.0
30-31	37.50603065574971	38.0	38.0	38.0	37.5	38.0
32-33	37.502507093947536	38.0	38.0	38.0	38.0	38.0
34-35	37.4851802845524	38.0	38.0	38.0	37.5	38.0
36-37	37.46346741423608	38.0	38.0	38.0	37.0	38.0
38-39	37.44260330031436	38.0	38.0	38.0	37.0	38.0
40-41	37.422203550330565	38.0	38.0	38.0	37.0	38.0
42-43	37.400458750554336	38.0	38.0	38.0	37.0	38.0
44-45	37.36974909355303	38.0	38.0	38.0	37.0	38.0
46-47	37.33142685234981	38.0	38.0	38.0	37.0	38.0
48-49	37.28523787258081	38.0	38.0	38.0	37.0	38.0
50-51	37.26711796636562	38.0	38.0	38.0	37.0	38.0
52-53	37.25748139118781	38.0	38.0	38.0	37.0	38.0
54-55	37.23659484329629	38.0	38.0	38.0	37.0	38.0
56-57	37.20620767604797	38.0	38.0	38.0	37.0	38.0
58-59	37.210129562390534	38.0	38.0	38.0	37.0	38.0
60-61	37.22881758174977	38.0	38.0	38.0	37.0	38.0
62-63	37.237865347197086	38.0	38.0	38.0	37.0	38.0
64-65	37.245709788372196	38.0	38.0	38.0	37.0	38.0
66-67	37.252988488873825	38.0	38.0	38.0	37.0	38.0
68-69	37.254197786409414	38.0	38.0	38.0	37.0	38.0
70-71	37.225297504472564	38.0	38.0	38.0	37.0	38.0
72-73	37.20551534920797	38.0	38.0	38.0	37.0	38.0
74-75	37.191677704357794	38.0	38.0	38.0	36.5	38.0
76-77	37.191717182746586	38.0	38.0	38.0	36.0	38.0
78-79	37.196142941642734	38.0	38.0	38.0	36.5	38.0
80-81	37.1852594843814	38.0	38.0	38.0	36.0	38.0
82-83	37.18881279376309	38.0	38.0	38.0	36.0	38.0
84-85	37.182720945225	38.0	38.0	38.0	36.0	38.0
86-87	37.155397117058655	38.0	38.0	38.0	36.0	38.0
88-89	37.112400582725336	38.0	38.0	38.0	36.0	38.0
90-91	37.11883400642291	38.0	38.0	38.0	36.0	38.0
92-93	37.100563356448816	38.0	38.0	38.0	36.0	38.0
94-95	37.083397191577255	38.0	38.0	38.0	36.0	38.0
96-97	37.07089275689924	38.0	38.0	38.0	36.0	38.0
98-99	37.105509236174264	38.0	38.0	38.0	36.0	38.0
100	35.51111002845928	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	16.0
20	46.0
21	169.0
22	442.0
23	1061.0
24	2329.0
25	4363.0
26	7602.0
27	12560.0
28	19823.0
29	29558.0
30	41283.0
31	56094.0
32	72050.0
33	95033.0
34	136489.0
35	244780.0
36	778851.0
37	5170577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45537153560197	19.124176979045362	14.048881716508102	23.371569768844573
2	14.33074349379429	20.55124365196597	42.22973960191688	22.88827325232286
3	18.602967213731368	25.74308094448646	30.247414668591	25.406537173191168
4	12.434616504491371	17.73736128883467	39.31034451754192	30.51767768913204
5	13.253096911188983	37.79570689332964	35.35552416062051	13.595672034860867
6	29.81111994921719	37.778257979321225	18.91416973519449	13.496452336267092
7	26.52512786730183	32.863938341368794	23.069192877883822	17.54174091344555
8	24.125698753912893	37.01924539447686	21.178175935157256	17.676879916452986
9	25.644615238910447	17.222193250301807	21.387765977214883	35.74542553357286
10-11	24.16996765460159	26.871549079266195	28.883311628121653	20.075171638010566
12-13	25.0571733290335	24.44675503800391	29.078652608094345	21.417419024868245
14-15	22.16080342519978	25.74558351861623	26.90957270569773	25.18404035048626
16-17	21.55889688330617	28.87825935929007	27.59915176223927	21.963691995164485
18-19	21.593063763640714	27.974118288437104	29.463989082183183	20.968828865738995
20-21	22.42678322117936	27.08676333370223	29.407623373053088	21.07883007206532
22-23	22.143831941228346	26.91871795811314	29.30935302579116	21.628097074867352
24-25	21.904368956010998	27.048737531348433	29.382109106888116	21.664784405752457
26-27	21.82544467542477	27.245125363010864	29.29404631085938	21.63538365070499
28-29	21.692743649428195	27.379152657363363	29.32338532237328	21.604718370835162
30-31	22.08778721871871	27.131199680515618	29.22984198444705	21.551171116318624
32-33	21.801669400679398	27.30856528581041	29.193503964700675	21.69626134880952
34-35	21.906620386003407	27.323044201320435	29.200845850214186	21.56948956246197
36-37	21.90525004737982	27.237621757055656	29.219808761119122	21.637319434445402
38-39	21.71121814206361	27.44044590168926	29.23588907263029	21.612446883616844
40-41	22.006326793869405	27.27664320555922	29.099720914586445	21.61730908598493
42-43	21.945061873023114	27.294966612613834	28.9312028707603	21.828768643602757
44-45	21.962999936181085	27.408764184763058	28.884044758953166	21.744191120102695
46-47	21.983734352659134	27.409813618190825	28.649049581105047	21.95740244804499
48-49	21.924571927063145	27.50411011450995	28.661630169207776	21.90968778921913
50-51	21.884977579064273	27.664562074794397	28.53388324461986	21.916577101521465
52-53	22.096037431210082	27.584565334948135	28.392836867740535	21.926560366101253
54-55	22.03544440388076	27.484516698058652	28.46124893789556	22.018789960165027
56-57	22.015931507720204	27.58989973961948	28.421990810855736	21.97217794180458
58-59	21.94978727865759	27.534545716658776	28.408087506532354	22.10757949815128
60-61	22.182889357439123	27.439512392655352	28.58339527297741	21.794202976928116
62-63	21.888548363139336	27.49364690110993	28.563273274144414	22.05453146160632
64-65	21.880224581738013	27.468315885896704	28.53946855492747	22.111990977437813
66-67	21.991739770816775	27.544791389554145	28.55278051707526	21.910688322553813
68-69	21.936890156158942	27.712425630673398	28.437461486017522	21.913222727150135
70-71	21.948456391244292	27.521374399730202	28.226244704920973	22.303924504104533
72-73	22.001432525459244	27.58789916036545	28.166779637196754	22.243888676978553
74-75	21.916546706033234	27.665865700769704	28.328976671966956	22.088610921230106
76-77	22.03837766352801	27.38581041257498	28.23025606505396	22.345555858843053
78-79	22.045497507490932	27.456478847748027	27.965911503080164	22.532112141680873
80-81	22.212207865202004	27.679424097160137	27.962085265286003	22.146282772351856
82-83	22.253386801545506	27.507324815197233	27.990785854962418	22.248502528294843
84-85	22.195954614724556	27.45032019298951	27.90145983723178	22.45226535505416
86-87	22.01706187219387	27.722446249848097	27.91566485516681	22.34482702279122
88-89	22.313284760395447	27.702758073502242	27.903166917294925	22.080790248807382
90-91	22.393640958781734	27.697136046347186	27.866537200385444	22.042685794485635
92-93	22.193106261378873	27.461360764524308	27.8581396374897	22.487393336607127
94-95	22.28509535071723	27.831261747800955	27.852804521912443	22.03083837956936
96-97	22.586338292508977	27.64967007729668	27.945396140458683	21.818595489735657
98-99	23.266641791958374	29.72665321445281	28.258729612098264	18.747975381490555
100	22.16963128670227	30.59363047486307	22.231327473841983	25.00541076459267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1418.0
1	1251.0
2	1734.0
3	2699.5
4	3345.0
5	4262.0
6	5281.5
7	6935.0
8	9422.5
9	11191.5
10	11287.5
11	11092.5
12	11981.5
13	13992.0
14	17646.5
15	21462.5
16	24173.5
17	25738.5
18	25362.5
19	23506.0
20	21589.5
21	20599.5
22	21704.0
23	25399.0
24	31815.5
25	41075.5
26	52975.0
27	66756.0
28	80896.0
29	96336.5
30	111680.5
31	126329.0
32	142479.0
33	158658.0
34	175407.0
35	190366.0
36	204921.5
37	218906.5
38	228384.5
39	237643.5
40	248796.0
41	256983.5
42	262022.5
43	268053.5
44	272276.5
45	275226.5
46	278112.5
47	279269.0
48	277850.5
49	272519.0
50	265742.0
51	257502.5
52	246803.5
53	234557.5
54	220106.5
55	203780.0
56	186482.0
57	167898.5
58	149132.0
59	130553.5
60	110485.0
61	89787.5
62	70614.0
63	54984.5
64	42483.5
65	31889.0
66	23302.0
67	17506.0
68	13376.5
69	9811.0
70	7165.5
71	5209.0
72	3704.0
73	2571.0
74	1741.5
75	1171.5
76	748.5
77	434.5
78	270.5
79	167.5
80	102.5
81	72.5
82	57.5
83	38.5
84	25.0
85	17.5
86	12.0
87	7.0
88	3.5
89	1.5
90	1.0
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006473724694966933
6	7.492736915470989E-5
7	0.0
8	0.0
9	1.4985473830941978E-5
10-11	7.492736915470989E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.894040618937975E-4
26-27	0.0
28-29	7.571525994585534E-4
30-31	0.0024837162758602886
32-33	1.4285969929386704E-4
34-35	4.52342239424295E-5
36-37	3.103141537237282E-4
38-39	5.480496018381583E-4
40-41	6.050720200375344E-4
42-43	2.235069332236043E-4
44-45	0.0
46-47	0.0014406781585283098
48-49	6.316871487276598E-5
50-51	0.0020137325416154147
52-53	0.0015074379232252243
54-55	0.0026491332811260236
56-57	0.0023768814094906757
58-59	0.0035875473773308614
60-61	0.00275340791936175
62-63	0.002942375080177643
64-65	0.004333069382079163
66-67	0.0016319952240885878
68-69	1.4509401708234538E-4
70-71	9.391748978022618E-4
72-73	0.006652556290409388
74-75	0.0061325537006408385
76-77	0.0049932224088385
78-79	0.00271699567488251
80-81	4.5123246476799475E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020814932745264604
90-91	3.9783159049944065E-4
92-93	0.0
94-95	9.67344107418533E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	191.0
22-23	372.0
24-25	501.0
26-27	1441.0
28-29	4191.0
30-31	12754.0
32-33	16531.0
34-35	22548.0
36-37	35551.0
38-39	40955.0
40-41	39981.0
42-43	45127.0
44-45	53150.0
46-47	54388.0
48-49	51470.0
50-51	47234.0
52-53	44204.0
54-55	47903.0
56-57	49009.0
58-59	27657.0
60-61	50192.0
62-63	49275.0
64-65	51919.0
66-67	54445.0
68-69	55332.0
70-71	54318.0
72-73	50807.0
74-75	51066.0
76-77	52731.0
78-79	53825.0
80-81	54122.0
82-83	50813.0
84-85	50422.0
86-87	52505.0
88-89	53922.0
90-91	54415.0
92-93	54440.0
94-95	63082.0
96-97	223931.0
98-99	442278.0
100-101	4454084.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.56929400335417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07390650646632	77.07560587882452
2	7.02652830437952	12.025101326006817
3	1.6396002396347353	4.208983048598239
4	0.5752462861740263	1.9689367434385145
5	0.25672375975313244	1.0983835437981124
6	0.14669289786432405	0.753144462333381
7	0.07753442822017008	0.4644196398627586
8	0.05434336753889664	0.37200988752545433
9	0.038839989828471345	0.29911594578477746
>10	0.10789761271874207	1.4010900007037812
>50	0.0017553895886544602	0.10307056419190097
>100	8.215892907381481E-4	0.1358146432263388
>500	8.222140676497862E-5	0.06071746729808403
>1k	2.7407135552353366E-5	0.03360684840732428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9970947661883952E-5
2	0.0	1.4985473830941976E-5	0.0	1.4985473830941976E-5	2.9970947661883952E-5
3	0.0	2.9970947661883952E-5	0.0	2.9970947661883952E-5	2.9970947661883952E-5
4	0.0	4.4956421492825927E-5	0.0	2.9970947661883952E-5	4.4956421492825927E-5
5	0.0	4.4956421492825927E-5	0.0	4.4956421492825927E-5	4.4956421492825927E-5
6	0.0	4.4956421492825927E-5	0.0	4.4956421492825927E-5	4.4956421492825927E-5
7	0.0	4.4956421492825927E-5	0.0	4.4956421492825927E-5	4.4956421492825927E-5
8	0.0	5.9941895323767904E-5	0.0	4.4956421492825927E-5	4.4956421492825927E-5
9	0.0	7.492736915470988E-5	0.0	7.492736915470988E-5	4.4956421492825927E-5
10-11	0.0	7.492736915470988E-5	0.0	9.740557990112284E-5	4.4956421492825927E-5
12-13	0.0	7.492736915470988E-5	0.0	1.1239105373206482E-4	4.4956421492825927E-5
14-15	0.0	1.348692644784778E-4	0.0	1.3486926447847777E-4	8.242010607018086E-5
16-17	0.0	1.4236200139394878E-4	0.0	1.798256859713037E-4	8.991284298565185E-5
18-19	0.0	1.4985473830941975E-4	0.0	2.1728937054865866E-4	1.0489831681659383E-4
20-21	0.0	1.6484021214036173E-4	0.0	2.3976758129507162E-4	1.0489831681659383E-4
22-23	0.0	1.723329490558327E-4	0.0	2.547530551260136E-4	1.1988379064753582E-4
24-25	0.0	2.0230389671771666E-4	0.0	3.221876873652525E-4	1.4985473830941975E-4
26-27	0.0	2.0979663363318766E-4	0.0	4.3457874109731733E-4	1.4985473830941975E-4
28-29	0.0	2.2478210746412964E-4	0.0	7.118100069697438E-4	1.723329490558327E-4
30-31	0.0	2.997094766188395E-4	0.0	0.0017308222274737984	1.798256859713037E-4
32-33	0.0	2.997094766188395E-4	0.0	0.0034616444549475964	2.0979663363318766E-4
34-35	0.0	3.2968042428072346E-4	0.0	0.005619552686603241	2.0979663363318766E-4
36-37	0.0	3.671441088580784E-4	0.0	0.009290993775184025	2.0979663363318766E-4
38-39	0.0	3.8962231960449137E-4	0.0	0.019128957345197435	2.0979663363318766E-4
40-41	0.0	3.8962231960449137E-4	0.0	0.029708701869842466	2.0979663363318766E-4
42-43	0.0	4.195932672663754E-4	0.0	0.03580029698212038	2.1728937054865866E-4
44-45	0.0	4.645496887592013E-4	0.0	0.04293338252564877	2.2478210746412964E-4
46-47	0.0	4.945206364210852E-4	0.0	0.05095061102520272	2.3976758129507162E-4
48-49	0.0	4.945206364210852E-4	0.0	0.059125186999981566	2.997094766188395E-4
50-51	0.0	5.619552686603241E-4	0.0	0.06664789486311444	2.997094766188395E-4
52-53	0.0	5.694480055757951E-4	0.0	0.08932091676932966	3.072022135343105E-4
54-55	0.0	5.694480055757951E-4	0.0	0.11751608578224698	3.146949504497815E-4
56-57	0.0	5.694480055757951E-4	0.0	0.1511659672696272	3.371731611961945E-4
58-59	0.0	5.99418953237679E-4	0.0	0.343894146209372	3.4466589811166546E-4
60-61	0.0	6.0691169015315E-4	0.0	0.5232253115442546	3.4466589811166546E-4
62-63	0.0	6.29389900899563E-4	0.0	0.5299238183466857	3.746368457735494E-4
64-65	0.0	6.893317962233309E-4	0.0	0.535768153140753	3.746368457735494E-4
66-67	0.0	6.893317962233309E-4	0.0	0.5407058667680484	3.746368457735494E-4
68-69	0.0	7.118100069697438E-4	0.0	0.5453363781818095	3.746368457735494E-4
70-71	0.0	7.193027438852148E-4	0.0	0.5498844694895003	3.8962231960449137E-4
72-73	0.0	7.193027438852148E-4	0.0	0.5536458234210668	4.195932672663753E-4
74-75	0.0	7.342882177161568E-4	0.0	0.556575483555016	4.195932672663753E-4
76-77	0.0	7.492736915470988E-4	0.0	0.5596849693749364	4.195932672663753E-4
78-79	0.0	7.642591653780408E-4	0.0	0.5617005156051981	4.195932672663753E-4
80-81	0.0	7.642591653780408E-4	0.0	0.5623373982430131	4.270860041818463E-4
82-83	0.0	7.642591653780408E-4	0.0	0.5628019479317723	4.3457874109731733E-4
84-85	0.0	8.017228499553957E-4	0.0	0.5630117445654055	4.3457874109731733E-4
86-87	0.0	8.391865345327508E-4	0.0	0.5631541065667994	4.3457874109731733E-4
88	0.0	9.141139036874605E-4	0.0	0.5631840775144613	4.3457874109731733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8925	0.0	51.74541	1
GTATCAA	16290	0.0	37.326694	1
TCAACGC	19090	0.0	31.492453	4
ATCAACG	19160	0.0	31.265493	3
CAACGCA	19570	0.0	30.741936	5
TATCAAC	19815	0.0	30.27527	2
AACGCAG	20270	0.0	29.699673	6
CGAGACC	4340	0.0	29.400463	86-87
ACCGAGG	4590	0.0	28.565979	90-91
AGACCGA	4575	0.0	28.122417	88-89
GAGACCG	4500	0.0	27.853292	88-89
ACGAGAC	4510	0.0	27.685114	86-87
GACCGAG	4630	0.0	27.53086	90-91
ATCTCCG	4285	0.0	27.377632	74-75
CCCACGA	4650	0.0	26.895817	82-83
TCTCCGA	4520	0.0	26.011406	74-75
ACGCAGA	23285	0.0	25.854088	7
GCCCACG	4850	0.0	25.620699	82-83
TCCGAGC	4670	0.0	25.582302	76-77
CGCAGAG	23775	0.0	25.303202	8
>>END_MODULE