FastQCFastQC Report
Fri 27 May 2016
SRR2138870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6173815
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99050.041.075771
GTATCAA166350.034.6931
ATCAACG199250.028.3284033
TCAACGC200950.028.1518234
CAACGCA202150.028.005615
TATCAAC205650.027.6933272
AACGCAG207950.027.2216286
ACGCAGA235600.023.991047
CGCAGAG236250.023.9429138
TACATGG176850.020.9511812
GTACATG181950.020.8515451
GCAGAGT275000.020.5998619
ACATGGG179050.019.9150813
GAGTACT162550.019.08855412-13
AGAGTAC237600.018.7752510-11
CATGGGG127450.018.3647024
CAGAGTA266250.017.90524110-11
AGTACTT174600.016.99692312-13
GTACTTT183650.016.88419514-15
ATGGGGG78100.015.5254725
Produced by FastQC (version 0.11.5)