##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6173815
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.521092063821154	33.0	31.0	33.0	18.0	34.0
2	32.09298869499653	33.0	32.0	33.0	31.0	34.0
3	32.20149955902469	33.0	33.0	33.0	30.0	34.0
4	32.70659681250572	33.0	33.0	34.0	31.0	34.0
5	32.73162590715789	33.0	33.0	34.0	32.0	34.0
6	36.16661788537557	38.0	36.0	38.0	33.0	38.0
7	37.145792998332475	38.0	38.0	38.0	36.0	38.0
8	37.02880504193922	38.0	38.0	38.0	36.0	38.0
9	37.33091986073441	38.0	38.0	38.0	37.0	38.0
10-11	37.43144206621028	38.0	38.0	38.0	37.0	38.0
12-13	37.45599949140038	38.0	38.0	38.0	37.0	38.0
14-15	37.48504644211076	38.0	38.0	38.0	37.0	38.0
16-17	37.493165490057606	38.0	38.0	38.0	37.0	38.0
18-19	37.50570004122248	38.0	38.0	38.0	37.0	38.0
20-21	37.512828829719254	38.0	38.0	38.0	37.0	38.0
22-23	37.53029663027516	38.0	38.0	38.0	38.0	38.0
24-25	37.49344290989358	38.0	38.0	38.0	37.5	38.0
26-27	37.50301507521711	38.0	38.0	38.0	37.5	38.0
28-29	37.49652088885276	38.0	38.0	38.0	37.0	38.0
30-31	37.48782467776472	38.0	38.0	38.0	37.5	38.0
32-33	37.48223103234838	38.0	38.0	38.0	37.0	38.0
34-35	37.46937901266949	38.0	38.0	38.0	37.0	38.0
36-37	37.44936154153914	38.0	38.0	38.0	37.0	38.0
38-39	37.42326002889599	38.0	38.0	38.0	37.0	38.0
40-41	37.40223169370668	38.0	38.0	38.0	37.0	38.0
42-43	37.38013668229303	38.0	38.0	38.0	37.0	38.0
44-45	37.34753292219729	38.0	38.0	38.0	37.0	38.0
46-47	37.30728619909529	38.0	38.0	38.0	37.0	38.0
48-49	37.262826004332155	38.0	38.0	38.0	37.0	38.0
50-51	37.24368462346641	38.0	38.0	38.0	37.0	38.0
52-53	37.23512287437005	38.0	38.0	38.0	37.0	38.0
54-55	37.21566963433959	38.0	38.0	38.0	37.0	38.0
56-57	37.18504119851585	38.0	38.0	38.0	37.0	38.0
58-59	37.19022478686202	38.0	38.0	38.0	37.0	38.0
60-61	37.20685692712691	38.0	38.0	38.0	37.0	38.0
62-63	37.21305235961347	38.0	38.0	38.0	37.0	38.0
64-65	37.21870504885976	38.0	38.0	38.0	37.0	38.0
66-67	37.22718626505285	38.0	38.0	38.0	37.0	38.0
68-69	37.228664256499286	38.0	38.0	38.0	37.0	38.0
70-71	37.1994555216645	38.0	38.0	38.0	37.0	38.0
72-73	37.17743293628308	38.0	38.0	38.0	36.0	38.0
74-75	37.160806445204855	38.0	38.0	38.0	36.0	38.0
76-77	37.16399326931645	38.0	38.0	38.0	36.0	38.0
78-79	37.16751881450517	38.0	38.0	38.0	36.0	38.0
80-81	37.15677850295414	38.0	38.0	38.0	36.0	38.0
82-83	37.15910258949506	38.0	38.0	38.0	36.0	38.0
84-85	37.1472796524404	38.0	38.0	38.0	36.0	38.0
86-87	37.120884413507994	38.0	38.0	38.0	36.0	38.0
88-89	37.08283559610999	38.0	38.0	38.0	36.0	38.0
90-91	37.088836926494906	38.0	38.0	38.0	36.0	38.0
92-93	37.067854860498656	38.0	38.0	38.0	36.0	38.0
94-95	37.05449457005908	38.0	38.0	38.0	36.0	38.0
96-97	37.04931895558792	38.0	38.0	38.0	36.0	38.0
98-99	37.08633517143413	38.0	38.0	38.0	36.0	38.0
100	35.44240750419917	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	21.0
20	48.0
21	147.0
22	445.0
23	1085.0
24	2395.0
25	4591.0
26	7927.0
27	13060.0
28	20054.0
29	29473.0
30	41215.0
31	54682.0
32	71833.0
33	95690.0
34	138183.0
35	242797.0
36	682842.0
37	4767321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.152739432587474	19.916210641232365	14.25863910726188	24.672410818918287
2	13.965465437496913	20.833617463432255	43.009711175342964	22.19120592372787
3	17.76476943348643	26.227284102293314	30.620661616844693	25.387284847375568
4	11.994690479063594	18.11035802012208	39.818442891469864	30.076508609344465
5	12.99225608882916	37.606550865463554	35.84523387570734	13.555959169999943
6	29.18022595767833	38.39029085924399	19.173683807295042	13.25579937578264
7	25.949125459703602	33.09200227088113	23.552778954341846	17.406093315073417
8	23.844187103112095	36.96344966604927	21.572253136836785	17.620110094001845
9	25.450831528128315	17.00676437612147	21.783892420471364	35.75851167527885
10-11	23.788570872767696	26.99876664560729	29.324411889021267	19.888250592603747
12-13	24.800313906393374	24.670361842717995	29.349332624965275	21.179991625923357
14-15	21.884944722185555	25.910000542614252	27.34124686275828	24.863807872441917
16-17	21.21542352662009	28.97187557450296	28.000895718449613	21.81180518042734
18-19	21.209851607150522	28.075250068231718	29.91072780768455	20.80417051693321
20-21	22.143698382933177	27.226485731672806	29.741683645925004	20.888132239469012
22-23	21.819437044937043	27.12291439855679	29.62849514348439	21.429153413021776
24-25	21.555594092562956	27.208673386199596	29.759799876834574	21.475932644402878
26-27	21.48733986489289	27.407559400987118	29.715779626614957	21.38932110750503
28-29	21.37948008442558	27.53073329082447	29.71525816353291	21.37452846121704
30-31	21.734420822571675	27.261359758560253	29.615799705875528	21.388419712992544
32-33	21.520572404252043	27.412659135362766	29.575634695436946	21.49113376494824
34-35	21.633353590641942	27.481684809873602	29.557319346273538	21.327642253210918
36-37	21.60595600528196	27.397653794206033	29.553389734128793	21.44300046638321
38-39	21.47510083157494	27.497656623561955	29.63867716626864	21.388565378594464
40-41	21.73843165641512	27.351171899171444	29.515969947618885	21.39442649679455
42-43	21.62445671466745	27.43052444285147	29.302716508235548	21.642302334245525
44-45	21.68972527122029	27.528407969048335	29.199269410523037	21.58259734920834
46-47	21.73533546036178	27.519431903074587	28.978953054076623	21.76627958248701
48-49	21.705641714941713	27.58817893279292	28.963711158887705	21.742468193377654
50-51	21.664559024110684	27.753122746270847	28.827825016244873	21.7544932133736
52-53	21.852418668106154	27.7086251887815	28.659846511012958	21.779109632099384
54-55	21.835215218490568	27.62165300606218	28.665358366918063	21.87777340852919
56-57	21.861234674137286	27.71831990292739	28.584408653624372	21.83603676931095
58-59	21.815779170105895	27.798919158902162	28.661496517888402	21.72380515310354
60-61	21.902376447055392	27.77947055936497	28.648940787652183	21.669212205927458
62-63	21.874870314187636	27.785669612148983	28.598104172458534	21.74135590120484
64-65	21.81949695952989	27.731271368782107	28.576627642688518	21.872604028999483
66-67	21.899348481303793	27.79931733173096	28.415509452855787	21.88582473410946
68-69	21.849338899828457	27.732558733855832	28.537859877733478	21.880242488582237
70-71	21.8941022609537	27.468675688843962	28.564572076821825	22.072649973380507
72-73	21.958598561310016	27.53641678579336	28.551947862891797	21.95303679000483
74-75	21.800781230150186	27.706112678357623	28.432745449762397	22.060360641729794
76-77	21.907929418229127	27.64522150571785	28.346603115500336	22.10024596055269
78-79	21.909719542354402	27.67025745934586	28.326079426905615	22.093943571394124
80-81	21.94736175177441	27.691739158311044	28.268899027313672	22.092000062600874
82-83	21.985386557442094	27.620505051340434	28.400104429866328	21.994003961351147
84-85	22.137188676752327	27.655362274559025	28.21701377419568	21.990435274492963
86-87	21.909893469777856	27.711019172559297	28.28113491264046	22.097952445022393
88-89	22.026401249790794	27.663954527719838	28.221746000822563	22.08789822166681
90-91	22.055687239238527	27.693124829578142	28.24176568586304	22.00942224532029
92-93	22.107724250363372	27.704245564902013	28.1610300860189	22.027000098715714
94-95	21.99732533026502	27.850822205384386	28.13098504962717	22.020867414723426
96-97	22.055466818251475	27.90368607700756	28.219245401904686	21.821601702836283
98-99	23.11869736378802	29.83531612031794	28.4188764976728	18.627110018221245
100	21.98086398765877	30.66298594865601	22.372794487042217	24.983355576643003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1443.0
1	1391.5
2	2002.5
3	2909.0
4	3613.5
5	4751.0
6	5850.5
7	7451.0
8	9508.5
9	10591.0
10	10549.5
11	10723.0
12	12000.5
13	14421.0
14	18751.5
15	23505.0
16	26797.0
17	28297.0
18	27476.5
19	25145.0
20	22892.0
21	21860.5
22	23091.5
23	27170.0
24	34209.0
25	44075.5
26	56919.5
27	70878.0
28	84732.5
29	99503.5
30	113364.0
31	127767.5
32	143701.0
33	158120.5
34	173293.0
35	187621.0
36	200876.0
37	213454.5
38	221724.0
39	228808.5
40	237599.5
41	244137.0
42	247827.5
43	253392.5
44	259038.0
45	263117.0
46	263512.5
47	262993.0
48	261818.0
49	255307.5
50	246698.0
51	235800.0
52	222822.5
53	209401.5
54	195217.5
55	179387.0
56	163701.0
57	147669.0
58	130636.5
59	113701.5
60	95207.5
61	76711.5
62	60235.0
63	46432.5
64	35229.0
65	26005.5
66	19169.5
67	14762.0
68	11296.5
69	8343.0
70	6112.5
71	4427.5
72	3168.0
73	2252.0
74	1545.0
75	1074.5
76	711.0
77	429.0
78	288.0
79	189.5
80	117.5
81	79.0
82	50.0
83	30.5
84	23.5
85	17.5
86	10.0
87	7.5
88	7.0
89	7.0
90	6.5
91	5.5
92	4.5
93	3.0
94	3.0
95	3.5
96	2.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.011451590305184072
6	6.478976127402587E-5
7	0.0
8	0.0
9	1.6197440318506466E-5
10-11	2.4296160477759698E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.641784812813849E-4
26-27	1.6201444877257042E-5
28-29	7.293663475877991E-4
30-31	0.0025799726620255284
32-33	2.1143729379781228E-4
34-35	4.078651075886974E-5
36-37	3.1955177537640534E-4
38-39	5.937782920924071E-4
40-41	6.478554365411896E-4
42-43	1.7569496141989641E-4
44-45	0.0
46-47	0.001525451216112719
48-49	1.1188214163074246E-4
50-51	0.001962983175062748
52-53	0.0016033630320558708
54-55	0.002739912239727119
56-57	0.0025964588940405645
58-59	0.0038017004231238522
60-61	0.002974154055541008
62-63	0.0030572782456474413
64-65	0.004532759616356125
66-67	0.0017700436133127133
68-69	1.4197646957714012E-4
70-71	7.367670184808917E-4
72-73	0.006489924078527501
74-75	0.005735962599569519
76-77	0.004542548571200598
78-79	0.002565950929433354
80-81	5.0484550717789344E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020484492025249984
90-91	2.778028868848616E-4
92-93	0.0
94-95	7.661295441879444E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	67.0
20-21	210.0
22-23	387.0
24-25	601.0
26-27	1907.0
28-29	4508.0
30-31	13875.0
32-33	17433.0
34-35	23956.0
36-37	36936.0
38-39	43558.0
40-41	42822.0
42-43	48045.0
44-45	57337.0
46-47	58740.0
48-49	54174.0
50-51	50466.0
52-53	48703.0
54-55	52815.0
56-57	53838.0
58-59	52940.0
60-61	51679.0
62-63	51423.0
64-65	54518.0
66-67	55982.0
68-69	57085.0
70-71	56942.0
72-73	52955.0
74-75	53061.0
76-77	54839.0
78-79	55969.0
80-81	55501.0
82-83	53529.0
84-85	52990.0
86-87	54866.0
88-89	55568.0
90-91	55457.0
92-93	55100.0
94-95	63034.0
96-97	183750.0
98-99	394989.0
100-101	3941260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.10845755841537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57795562823843	78.90106003570438
2	6.889120358434021	12.002012967149305
3	1.5309385626938372	4.000730904388726
4	0.5077646528385829	1.769223828458128
5	0.21112579289502956	0.9195421084941738
6	0.11015183283284732	0.5757093753181046
7	0.05483565778736174	0.3343654698340733
8	0.03432016923496256	0.23916616041610955
9	0.020714019053085626	0.16239296245949247
>10	0.060928024233961375	0.8004202096707691
>50	0.0011736145127986355	0.06699822776994976
>100	8.366598283982004E-4	0.13164683538252267
>500	1.0802194950726273E-4	0.0681256751666445
>1k	2.7005467135199212E-5	0.028605239787546848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6197440318506466E-5	0.0	0.0	0.0
3	0.0	1.6197440318506466E-5	0.0	0.0	0.0
4	0.0	4.8592320955519396E-5	0.0	0.0	0.0
5	0.0	4.8592320955519396E-5	0.0	0.0	0.0
6	0.0	4.8592320955519396E-5	0.0	0.0	1.6197440318506466E-5
7	0.0	4.8592320955519396E-5	0.0	0.0	1.6197440318506466E-5
8	0.0	6.478976127402587E-5	0.0	0.0	1.6197440318506466E-5
9	0.0	1.6197440318506466E-4	0.0	0.0	1.6197440318506466E-5
10-11	0.0	1.6197440318506466E-4	0.0	4.8592320955519396E-5	1.6197440318506466E-5
12-13	0.0	1.6197440318506466E-4	0.0	4.8592320955519396E-5	1.6197440318506466E-5
14-15	0.0	2.42961604777597E-4	0.0	5.669104111477263E-5	3.239488063701293E-5
16-17	0.0	2.42961604777597E-4	0.0	6.478976127402587E-5	4.8592320955519396E-5
18-19	0.0	2.5915904509610346E-4	0.0	8.098720159253233E-5	4.8592320955519396E-5
20-21	0.0	2.753564854146099E-4	0.0	1.2148080238879851E-4	6.478976127402587E-5
22-23	0.0	3.644424071663955E-4	0.0	2.0246800398133084E-4	1.1338208222954526E-4
24-25	2.42961604777597E-5	4.211334482811681E-4	0.0	2.5915904509610346E-4	1.2957952254805173E-4
26-27	3.239488063701293E-5	4.616270490774343E-4	0.0	3.158500862108761E-4	1.5387568302581143E-4
28-29	3.239488063701293E-5	4.85923209555194E-4	0.0	5.183180901922069E-4	1.6197440318506466E-4
30-31	3.239488063701293E-5	5.831078514662328E-4	0.0	0.0014496709085063287	1.6197440318506466E-4
32-33	6.478976127402587E-5	5.91206571625486E-4	0.0	0.0033123765451345726	1.7817184350357113E-4
34-35	8.908592175178555E-5	6.802924933772716E-4	0.0	0.005677202831636516	1.7817184350357113E-4
36-37	1.0528336207029203E-4	7.612796949698039E-4	0.0	0.009613180829033587	1.7817184350357113E-4
38-39	1.1338208222954527E-4	7.774771352883103E-4	0.0	0.014529103965700299	1.7817184350357113E-4
40-41	1.2957952254805173E-4	8.908592175178556E-4	0.0	0.01980946950953341	1.7817184350357113E-4
42-43	1.2957952254805173E-4	9.232540981548687E-4	0.0	0.02466870160508535	1.7817184350357113E-4
44-45	1.2957952254805173E-4	0.001044734900543667	0.0	0.030661754522932738	1.7817184350357113E-4
46-47	1.2957952254805173E-4	0.0011338208222954527	0.0	0.03746467945670546	1.7817184350357113E-4
48-49	1.2957952254805173E-4	0.0011338208222954527	0.0	0.0443323941517522	1.9436928382207758E-4
50-51	1.2957952254805173E-4	0.0012795977851620109	0.0	0.050625099715491964	1.9436928382207758E-4
52-53	1.6197440318506466E-4	0.0012795977851620109	0.0	0.05680442319700218	2.3486288461834376E-4
54-55	1.6197440318506466E-4	0.001287696505321264	0.0	0.06357495325013787	2.753564854146099E-4
56-57	1.6197440318506466E-4	0.0013119926657990239	0.0	0.07010252169849598	2.753564854146099E-4
58-59	1.6197440318506466E-4	0.001320091385958277	0.0	0.07696213767338347	2.753564854146099E-4
60-61	1.6197440318506466E-4	0.0013443875464360367	0.0	0.08334392915887502	2.753564854146099E-4
62-63	1.6197440318506466E-4	0.0013767824270730497	0.0	0.0895637462411815	2.753564854146099E-4
64-65	1.6197440318506466E-4	0.0016926325132839257	0.0	0.0955406017187104	2.753564854146099E-4
66-67	1.6197440318506466E-4	0.0017169286737616855	0.0	0.1019466893646797	2.8345520557386316E-4
68-69	1.6197440318506466E-4	0.001741224834239445	0.0	0.1083041846896935	2.915539257331164E-4
70-71	1.6197440318506466E-4	0.0017574222745579517	0.0	0.11351976047225257	2.915539257331164E-4
72-73	1.6197440318506466E-4	0.001773619714876458	0.0	0.11753672567124218	3.0775136605162287E-4
74-75	1.6197440318506466E-4	0.0018627056366282437	0.0	0.12071142397366943	3.0775136605162287E-4
76-77	1.6197440318506466E-4	0.001943692838220776	0.0	0.12387802355593745	3.0775136605162287E-4
78-79	1.7817184350357113E-4	0.0019922851591762953	0.0	0.12608897415941359	3.0775136605162287E-4
80-81	1.7817184350357113E-4	0.0019922851591762953	0.0	0.1269717346567722	3.158500862108761E-4
82-83	1.7817184350357113E-4	0.0020003838793355485	0.0	0.12756294122839767	3.3204752652938257E-4
84-85	1.7817184350357113E-4	0.002024680039813308	0.0	0.12783829771381228	3.401462466886358E-4
86-87	1.7817184350357113E-4	0.002024680039813308	0.0	0.12792738363556408	3.401462466886358E-4
88	1.7817184350357113E-4	0.0021056672414058405	0.0	0.12792738363556408	3.401462466886358E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9905	0.0	41.07577	1
GTATCAA	16635	0.0	34.693	1
ATCAACG	19925	0.0	28.328403	3
TCAACGC	20095	0.0	28.151823	4
CAACGCA	20215	0.0	28.00561	5
TATCAAC	20565	0.0	27.693327	2
AACGCAG	20795	0.0	27.221628	6
ACGCAGA	23560	0.0	23.99104	7
CGCAGAG	23625	0.0	23.942913	8
TACATGG	17685	0.0	20.951181	2
GTACATG	18195	0.0	20.851545	1
GCAGAGT	27500	0.0	20.599861	9
ACATGGG	17905	0.0	19.915081	3
GAGTACT	16255	0.0	19.088554	12-13
AGAGTAC	23760	0.0	18.77525	10-11
CATGGGG	12745	0.0	18.364702	4
CAGAGTA	26625	0.0	17.905241	10-11
AGTACTT	17460	0.0	16.996923	12-13
GTACTTT	18365	0.0	16.884195	14-15
ATGGGGG	7810	0.0	15.525472	5
>>END_MODULE