FastQCFastQC Report
Fri 27 May 2016
SRR2138869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8099311
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107400.051.462921
CTTATAC79250.039.8909761
GTATCAA206250.039.840051
ATCAACG253350.031.8186593
TCAACGC254500.031.7741224
CAACGCA259400.031.1414625
TATACAC111250.030.9518973
TATCAAC263600.030.836912
AACGCAG268600.030.1026296
TTATACA119100.027.78082
ACGCAGA303300.026.6308867
CGCAGAG305050.026.4505148
ACACATC133500.023.7408776
ACATCTC135150.023.6690398
TACACAT152300.023.3555875
GCAGAGT355100.022.7224149
GTACATG248750.022.1180321
TACATGG244300.022.003992
ACATGGG245800.021.1846773
TATGCCG83550.021.16497442-43
Produced by FastQC (version 0.11.5)