##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8099311
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.57607813800458	33.0	31.0	33.0	18.0	34.0
2	32.099772313965964	33.0	32.0	33.0	31.0	34.0
3	32.191697664159335	33.0	33.0	33.0	30.0	34.0
4	32.69287189984432	33.0	33.0	34.0	31.0	34.0
5	32.7303831153045	33.0	33.0	34.0	32.0	34.0
6	36.133387765947006	38.0	36.0	38.0	33.0	38.0
7	37.15686210839416	38.0	38.0	38.0	36.0	38.0
8	37.03829943065527	38.0	38.0	38.0	36.0	38.0
9	37.343896783318975	38.0	38.0	38.0	37.0	38.0
10-11	37.444368724697696	38.0	38.0	38.0	37.0	38.0
12-13	37.467786395657605	38.0	38.0	38.0	37.0	38.0
14-15	37.49649235595472	38.0	38.0	38.0	37.0	38.0
16-17	37.50385890849233	38.0	38.0	38.0	37.0	38.0
18-19	37.519474434306815	38.0	38.0	38.0	37.5	38.0
20-21	37.52797530720673	38.0	38.0	38.0	38.0	38.0
22-23	37.54531450589428	38.0	38.0	38.0	38.0	38.0
24-25	37.50832372303577	38.0	38.0	38.0	38.0	38.0
26-27	37.5183399767033	38.0	38.0	38.0	38.0	38.0
28-29	37.51332762698627	38.0	38.0	38.0	38.0	38.0
30-31	37.502253768406554	38.0	38.0	38.0	38.0	38.0
32-33	37.49314561895581	38.0	38.0	38.0	38.0	38.0
34-35	37.473910671595604	38.0	38.0	38.0	37.5	38.0
36-37	37.45122690520248	38.0	38.0	38.0	37.0	38.0
38-39	37.410798641705384	38.0	38.0	38.0	37.0	38.0
40-41	37.40885794733489	38.0	38.0	38.0	37.0	38.0
42-43	37.39105371299954	38.0	38.0	38.0	37.0	38.0
44-45	37.35667633534237	38.0	38.0	38.0	37.0	38.0
46-47	37.31434542651899	38.0	38.0	38.0	37.0	38.0
48-49	37.267209296377395	38.0	38.0	38.0	37.0	38.0
50-51	37.24931502776159	38.0	38.0	38.0	37.0	38.0
52-53	37.24215426904646	38.0	38.0	38.0	37.0	38.0
54-55	37.22291766612435	38.0	38.0	38.0	37.0	38.0
56-57	37.19280657596866	38.0	38.0	38.0	37.0	38.0
58-59	37.197017464716254	38.0	38.0	38.0	37.0	38.0
60-61	37.213729764784645	38.0	38.0	38.0	37.0	38.0
62-63	37.221219686976866	38.0	38.0	38.0	37.0	38.0
64-65	37.230078096609475	38.0	38.0	38.0	37.0	38.0
66-67	37.236260384067734	38.0	38.0	38.0	37.0	38.0
68-69	37.237030366667675	38.0	38.0	38.0	37.0	38.0
70-71	37.20230093445188	38.0	38.0	38.0	37.0	38.0
72-73	37.178812818869886	38.0	38.0	38.0	36.5	38.0
74-75	37.16911324215672	38.0	38.0	38.0	36.5	38.0
76-77	37.17493861692619	38.0	38.0	38.0	36.0	38.0
78-79	37.18442395475947	38.0	38.0	38.0	36.0	38.0
80-81	37.177722748930634	38.0	38.0	38.0	36.0	38.0
82-83	37.18079777420297	38.0	38.0	38.0	36.0	38.0
84-85	37.176081009830185	38.0	38.0	38.0	36.0	38.0
86-87	37.15044639784679	38.0	38.0	38.0	36.0	38.0
88-89	37.116875912100426	38.0	38.0	38.0	36.0	38.0
90-91	37.12465271369609	38.0	38.0	38.0	36.0	38.0
92-93	37.10662099826223	38.0	38.0	38.0	36.0	38.0
94-95	37.09178041489922	38.0	38.0	38.0	36.0	38.0
96-97	37.08781642756347	38.0	38.0	38.0	36.0	38.0
98-99	37.129725081271545	38.0	38.0	38.0	36.0	38.0
100	35.52413873865618	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	15.0
19	21.0
20	87.0
21	179.0
22	508.0
23	1270.0
24	2939.0
25	5497.0
26	9878.0
27	16026.0
28	25594.0
29	37923.0
30	53158.0
31	71152.0
32	92917.0
33	122508.0
34	178664.0
35	314648.0
36	892015.0
37	6274307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82371944971616	20.126995987683397	14.246211807399419	24.80307275520103
2	13.888601141504505	20.68017884484248	43.31116560408657	22.120054409566446
3	17.605744488636134	26.415358047122773	30.882404688497576	25.096492775743517
4	11.93148404845795	18.299791179768256	39.454664723949975	30.314060047823823
5	12.847815021889666	37.70210001010083	35.94186316162	13.508221806389505
6	29.02446567946262	38.38518243231981	19.090884958647685	13.499466929569884
7	25.75926838212144	33.111771249649664	23.347198711989044	17.78176165623985
8	23.723045543385794	37.10276554422538	21.658128902338593	17.51606001005024
9	25.342862540142306	16.95887674382139	21.548317078862272	36.14994363717403
10-11	23.773804421228746	26.98715242330226	29.298886528536784	19.94015662693221
12-13	24.763612608529293	24.505195565400566	29.326278494553428	21.404913331516717
14-15	21.874150776529017	25.800984799818156	27.06595659803655	25.258907825616276
16-17	21.30923852658578	29.09953698530653	27.682867098201314	21.908357389906378
18-19	21.337833551520617	27.91475719354399	29.72452224639849	21.0228870085369
20-21	22.117445238197835	27.07152534449952	29.581880362943906	21.22914905435874
22-23	21.83787064149555	27.211921523305755	29.43615634837291	21.514051486825785
24-25	21.73980474915857	27.316564759743372	29.56692819559609	21.376702295501964
26-27	21.687732329153132	27.507814953461	29.475980402629563	21.328472314756304
28-29	21.443375674874872	27.578076218480795	29.50462860904296	21.47391949760137
30-31	22.090698829837127	27.24601307092549	29.469072102796503	21.194215996440878
32-33	21.362350005075907	27.621278551049684	29.467532971396547	21.54883847247786
34-35	21.681792055524866	27.535350491147753	29.56182685305751	21.22103060026987
36-37	21.51351905552996	27.53142849007752	29.516605614425156	21.438446839967366
38-39	21.41260895019933	27.295083883452286	29.729588075048284	21.562719091300096
40-41	21.64296658446964	27.156424973151832	29.699283456054992	21.501324986323528
42-43	21.798582354992178	27.13924220428173	29.483512247033193	21.5786631936929
44-45	21.581406337272014	27.484378410099158	29.329807525242046	21.60440772738678
46-47	21.8842616120762	27.24322726984895	28.95737381248894	21.915137305585908
48-49	21.772433010625665	27.318994399959017	28.943867547449937	21.96470504196538
50-51	21.584068638631262	27.494255518737234	28.9838847331361	21.937791109495404
52-53	21.73950876743085	27.462744178809245	29.079581706365026	21.71816534739488
54-55	21.75780301236213	27.413054602306016	28.8279640128132	22.001178372518655
56-57	21.98014042609401	27.67426304732487	28.484094424733364	21.86150210184776
58-59	21.621260167306563	27.930538616961247	28.887373907882818	21.56082730784937
60-61	21.89498786651467	28.166589961721517	28.42051049771047	21.517911674053348
62-63	21.632806608344424	28.438827868742806	28.314764168655188	21.61360135425758
64-65	21.594031333672962	28.409184085169475	28.310287847549105	21.686496733608458
66-67	21.705323410612475	28.307580972949737	28.251827739394557	21.735267877043228
68-69	21.700788976142338	28.198606512866007	28.35643796779685	21.744166543194805
70-71	21.79533863969367	27.86666232224375	28.4201652847439	21.91783375331868
72-73	21.900160898709757	27.641549541688104	28.50150512143348	21.956784438168658
74-75	21.859530620583214	27.797860402573825	28.362198719228342	21.98041025761461
76-77	21.92666917295693	27.72901265326585	28.308944176911528	22.035373996865694
78-79	21.874708075035045	27.737407809422766	28.27411883098585	22.113765284556337
80-81	21.942415886131744	27.759536213382326	28.25284077087594	22.045207129609988
82-83	21.980972872283335	27.72907355729742	28.31528018503483	21.97467338538442
84-85	22.13016566994399	27.680692446889545	28.190023494234257	21.999118388932214
86-87	21.911471149389115	27.745421612969622	28.26663763282259	22.076469604818673
88-89	22.0568800584231	27.714344478236303	28.135438007838747	22.09333745550185
90-91	22.114458044485847	27.68214620030302	28.189416921170835	22.013978834040298
92-93	22.115277528479176	27.72091450611744	28.165327411390003	21.998480554013383
94-95	21.998493548555334	27.84722239245148	28.13677179339993	22.017512265593258
96-97	22.104699131988195	27.885785984395934	28.20073121064044	21.808783672975427
98-99	23.14107428479324	29.812168540159384	28.407385574485204	18.639371600562175
100	22.048078907718406	30.62146799720044	22.434095065095185	24.89635802998597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1761.0
1	1696.5
2	2512.0
3	3749.0
4	4799.5
5	6401.0
6	7798.5
7	9831.0
8	12655.0
9	14372.0
10	14526.0
11	14610.0
12	16033.5
13	19164.0
14	25079.5
15	31391.0
16	35556.5
17	37621.0
18	36678.5
19	33764.5
20	30809.0
21	29033.5
22	30189.0
23	34992.0
24	43337.5
25	55271.5
26	70915.5
27	88803.5
28	106943.0
29	126466.5
30	146108.5
31	166313.5
32	188043.0
33	208617.5
34	228500.5
35	247250.0
36	266778.5
37	284758.0
38	295921.5
39	304745.5
40	315128.0
41	321525.0
42	327070.5
43	349840.5
44	362980.5
45	358165.0
46	349607.5
47	338042.5
48	332462.5
49	323447.5
50	311981.0
51	300632.5
52	287104.0
53	272553.0
54	257100.5
55	237778.0
56	216587.5
57	193269.5
58	169777.5
59	147838.5
60	124667.5
61	102371.5
62	80997.0
63	62308.5
64	47602.0
65	35969.0
66	26866.0
67	20391.5
68	15702.0
69	11756.0
70	8706.0
71	6388.5
72	4679.5
73	3371.5
74	2355.0
75	1579.5
76	1008.5
77	610.0
78	402.0
79	265.5
80	171.5
81	104.5
82	60.5
83	39.5
84	31.0
85	26.0
86	18.0
87	11.5
88	7.5
89	3.0
90	1.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.011963980639834672
6	7.408037547885246E-5
7	1.234672924647541E-5
8	1.234672924647541E-5
9	1.234672924647541E-5
10-11	1.8520093869713115E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.420977828487038E-4
26-27	0.0
28-29	7.288462715591028E-4
30-31	0.0023001430726090244
32-33	1.9832566040275728E-4
34-35	3.108253700391571E-5
36-37	2.997135113473409E-4
38-39	5.593120587365643E-4
40-41	6.076285101069192E-4
42-43	1.2113230912353252E-4
44-45	0.0
46-47	0.001532720232251278
48-49	7.215443568081449E-5
50-51	0.002098668676092271
52-53	0.0014023952911572967
54-55	0.0027151013804033552
56-57	0.0024554170070071888
58-59	0.0037640327847255546
60-61	0.002732864126920314
62-63	0.00292762989939389
64-65	0.004202152987939467
66-67	0.0017315257825977794
68-69	1.3041509748927028E-4
70-71	8.512648107234708E-4
72-73	0.006641004989538927
74-75	0.005765518615926289
76-77	0.004496511665594656
78-79	0.0027470009789942146
80-81	6.227409585213266E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020045462456967445
90-91	3.216776064546674E-4
92-93	0.0
94-95	6.76220592962623E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	58.0
20-21	192.0
22-23	483.0
24-25	686.0
26-27	1850.0
28-29	5404.0
30-31	17928.0
32-33	22738.0
34-35	31186.0
36-37	48067.0
38-39	57420.0
40-41	55660.0
42-43	63797.0
44-45	75439.0
46-47	77431.0
48-49	71935.0
50-51	66067.0
52-53	64433.0
54-55	69726.0
56-57	71674.0
58-59	70685.0
60-61	70568.0
62-63	69800.0
64-65	76221.0
66-67	85168.0
68-69	88619.0
70-71	100586.0
72-73	74863.0
74-75	70924.0
76-77	72897.0
78-79	74931.0
80-81	74213.0
82-83	70804.0
84-85	70066.0
86-87	72446.0
88-89	73819.0
90-91	73456.0
92-93	72590.0
94-95	82318.0
96-97	237670.0
98-99	507971.0
100-101	5106522.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.49590185722143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17152156431732	75.3462813455766
2	7.438110778928688	12.569797567589985
3	1.9144691381041827	4.852943892057909
4	0.725957615526355	2.453617737360695
5	0.31267507458187294	1.3209881207534664
6	0.15746736926761162	0.7983208427610574
7	0.08487478883974292	0.5020100279568717
8	0.052982098963830604	0.35814161873899386
9	0.03598349653060724	0.27364121921968715
>10	0.1043638061119546	1.3222901509486256
>50	0.0012067677517148727	0.06520075325630545
>100	2.945919844886153E-4	0.05461190410198384
>500	4.645455998300178E-5	0.03116624371391227
>1k	4.645453167446164E-5	0.05098857596395583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.234672924647541E-5	0.0	0.0	0.0
3	0.0	2.469345849295082E-5	0.0	0.0	0.0
4	0.0	2.469345849295082E-5	0.0	0.0	0.0
5	0.0	6.173364623237705E-5	0.0	0.0	0.0
6	1.234672924647541E-5	6.173364623237705E-5	0.0	0.0	0.0
7	1.234672924647541E-5	6.173364623237705E-5	0.0	0.0	0.0
8	1.234672924647541E-5	7.408037547885246E-5	0.0	0.0	0.0
9	1.234672924647541E-5	9.877383397180328E-5	0.0	2.469345849295082E-5	0.0
10-11	6.173364623237705E-5	9.877383397180328E-5	0.0	2.469345849295082E-5	0.0
12-13	8.642710472532787E-5	9.877383397180328E-5	0.0	2.469345849295082E-5	0.0
14-15	9.260046934856557E-5	1.6050748020418032E-4	0.0	2.469345849295082E-5	0.0
16-17	1.2346729246475412E-4	1.6050748020418032E-4	0.0	3.704018773942623E-5	0.0
18-19	1.3581402171122952E-4	1.7285420945065572E-4	0.0	4.938691698590164E-5	0.0
20-21	1.6050748020418032E-4	1.8520093869713115E-4	0.0	8.025374010209016E-5	0.0
22-23	1.6050748020418032E-4	1.9754766794360655E-4	0.0	1.0494719859504098E-4	0.0
24-25	2.1606776181331967E-4	2.2841449105979507E-4	0.0	1.543341155809426E-4	0.0
26-27	2.469345849295082E-4	2.469345849295082E-4	0.0	2.1606776181331967E-4	0.0
28-29	2.469345849295082E-4	2.469345849295082E-4	0.0	4.691757113660656E-4	0.0
30-31	2.71628043422459E-4	2.9632150191540984E-4	0.0	0.0015248210619397131	0.0
32-33	2.839747726689344E-4	2.9632150191540984E-4	0.0	0.0038953930772629916	0.0
34-35	3.271883250315984E-4	3.1484159578512295E-4	0.0	0.0073277838077831555	0.0
36-37	3.704018773942623E-4	3.3336168965483607E-4	0.0	0.012124488120038853	0.0
38-39	3.704018773942623E-4	3.3336168965483607E-4	0.0	0.019569565855663523	0.0
40-41	4.1978879438016396E-4	3.704018773942623E-4	0.0	0.027477645938031026	0.0
42-43	4.81522440612541E-4	3.8274860664073767E-4	0.0	0.03518817835245492	0.0
44-45	5.309093575984426E-4	4.321355236266394E-4	0.0	0.0438308888249877	0.0
46-47	5.309093575984426E-4	4.568289821195902E-4	0.0	0.05375765913915394	0.0
48-49	5.43256086844918E-4	4.568289821195902E-4	0.0	0.06296214579240135	0.0
50-51	5.679495453378688E-4	5.062158991054918E-4	0.0	0.07264198152163807	0.0
52-53	5.741229099611065E-4	5.123892637287295E-4	0.0	0.08214896304142413	0.0
54-55	6.235098269470082E-4	5.556028160913934E-4	0.0	0.09099539454652378	0.0
56-57	6.420299208167213E-4	5.926430038308197E-4	0.0	0.09728605309760299	0.0
58-59	6.420299208167213E-4	6.235098269470082E-4	0.0	0.10350263127320336	0.0
60-61	6.420299208167213E-4	6.358565561934836E-4	0.0	0.10920682018507501	0.0
62-63	6.420299208167213E-4	6.543766500631968E-4	0.0	0.11475050161674247	0.0
64-65	6.420299208167213E-4	7.408037547885246E-4	0.0	0.12039295688238172	0.0
66-67	6.420299208167213E-4	7.408037547885246E-4	0.0	0.12583169111545414	0.0
68-69	6.667233793096721E-4	7.654972132814755E-4	0.0	0.13112226459756887	0.0
70-71	6.667233793096721E-4	7.654972132814755E-4	0.0	0.13595600909756397	0.0
72-73	6.667233793096721E-4	7.654972132814755E-4	0.0	0.14009833675975647	0.0
74-75	6.667233793096721E-4	7.654972132814755E-4	0.0	0.14333317982233304	0.0
76-77	6.667233793096721E-4	7.716705779047131E-4	0.0	0.1465680228849096	0.0
78-79	6.667233793096721E-4	7.778439425279508E-4	0.0	0.14858671311670832	0.0
80-81	6.667233793096721E-4	7.901906717744262E-4	0.0	0.14939542388235244	0.0
82-83	6.667233793096721E-4	7.901906717744262E-4	0.0	0.1497781724889932	0.0
84-85	6.667233793096721E-4	8.025374010209017E-4	0.0	0.14998806688618327	0.0
86-87	6.728967439329098E-4	8.395775887603279E-4	0.0	0.1501053608140248	0.0
88	6.91416837802623E-4	9.136579642391804E-4	0.0	0.15011153417864803	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10740	0.0	51.46292	1
CTTATAC	7925	0.0	39.890976	1
GTATCAA	20625	0.0	39.84005	1
ATCAACG	25335	0.0	31.818659	3
TCAACGC	25450	0.0	31.774122	4
CAACGCA	25940	0.0	31.141462	5
TATACAC	11125	0.0	30.951897	3
TATCAAC	26360	0.0	30.83691	2
AACGCAG	26860	0.0	30.102629	6
TTATACA	11910	0.0	27.7808	2
ACGCAGA	30330	0.0	26.630886	7
CGCAGAG	30505	0.0	26.450514	8
ACACATC	13350	0.0	23.740877	6
ACATCTC	13515	0.0	23.669039	8
TACACAT	15230	0.0	23.355587	5
GCAGAGT	35510	0.0	22.722414	9
GTACATG	24875	0.0	22.118032	1
TACATGG	24430	0.0	22.00399	2
ACATGGG	24580	0.0	21.184677	3
TATGCCG	8355	0.0	21.164974	42-43
>>END_MODULE