FastQCFastQC Report
Fri 27 May 2016
SRR2138868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474491
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83300.049.5846941
GTATCAA158850.038.3863371
TCAACGC189350.031.6409594
ATCAACG189200.031.5760213
CAACGCA191550.031.2330975
TATCAAC198600.030.5531862
AACGCAG198350.030.202046
ACGCAGA229200.026.025437
CGCAGAG237600.025.0874218
GCAGAGT278600.021.3343169
GTACATG202250.021.264431
TACATGG203750.020.5436122
ACATGGG205350.019.7407263
GAGTACT164100.019.48523912-13
GTGGTAT44700.019.3379041
AGAGTAC247900.018.66927310-11
CAGAGTA272350.018.26735910-11
CATGGGG162600.018.0958254
TGGTATC45300.017.671792
GTACTTT185800.017.25534414-15
Produced by FastQC (version 0.11.5)