##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7474491
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.748898754443612	33.0	31.0	33.0	25.0	34.0
2	32.11487765521425	33.0	32.0	33.0	31.0	34.0
3	32.21944183222644	33.0	33.0	33.0	30.0	34.0
4	32.704125806024784	33.0	33.0	34.0	31.0	34.0
5	32.73264895228317	33.0	33.0	34.0	32.0	34.0
6	36.13317682769301	38.0	36.0	38.0	33.0	38.0
7	37.12079163651411	38.0	38.0	38.0	36.0	38.0
8	36.997049698768784	38.0	38.0	38.0	35.0	38.0
9	37.30623316022456	38.0	38.0	38.0	37.0	38.0
10-11	37.4072494033373	38.0	38.0	38.0	37.0	38.0
12-13	37.43694172619915	38.0	38.0	38.0	37.0	38.0
14-15	37.465319979648115	38.0	38.0	38.0	37.0	38.0
16-17	37.472755937494604	38.0	38.0	38.0	37.0	38.0
18-19	37.48623879539088	38.0	38.0	38.0	37.0	38.0
20-21	37.495699116930105	38.0	38.0	38.0	37.0	38.0
22-23	37.51190298006007	38.0	38.0	38.0	37.5	38.0
24-25	37.474256800270865	38.0	38.0	38.0	37.0	38.0
26-27	37.48596337430539	38.0	38.0	38.0	37.0	38.0
28-29	37.47974201576982	38.0	38.0	38.0	37.0	38.0
30-31	37.47133612664111	38.0	38.0	38.0	37.0	38.0
32-33	37.46853485117759	38.0	38.0	38.0	37.0	38.0
34-35	37.45822889580698	38.0	38.0	38.0	37.0	38.0
36-37	37.437767951985094	38.0	38.0	38.0	37.0	38.0
38-39	37.41543604154786	38.0	38.0	38.0	37.0	38.0
40-41	37.39711942603349	38.0	38.0	38.0	37.0	38.0
42-43	37.381980990207104	38.0	38.0	38.0	37.0	38.0
44-45	37.35598320292515	38.0	38.0	38.0	37.0	38.0
46-47	37.32396009736826	38.0	38.0	38.0	37.0	38.0
48-49	37.286221182994595	38.0	38.0	38.0	37.0	38.0
50-51	37.27261239758351	38.0	38.0	38.0	37.0	38.0
52-53	37.26110871600155	38.0	38.0	38.0	37.0	38.0
54-55	37.24051781146527	38.0	38.0	38.0	37.0	38.0
56-57	37.209428637767985	38.0	38.0	38.0	37.0	38.0
58-59	37.213723713147445	38.0	38.0	38.0	37.0	38.0
60-61	37.22900002664454	38.0	38.0	38.0	37.0	38.0
62-63	37.23463533644127	38.0	38.0	38.0	37.0	38.0
64-65	37.23968625860132	38.0	38.0	38.0	37.0	38.0
66-67	37.244182147461046	38.0	38.0	38.0	37.0	38.0
68-69	37.24391302410834	38.0	38.0	38.0	37.0	38.0
70-71	37.210970761824115	38.0	38.0	38.0	37.0	38.0
72-73	37.18571350757334	38.0	38.0	38.0	36.0	38.0
74-75	37.16893343528912	38.0	38.0	38.0	36.0	38.0
76-77	37.17165326099016	38.0	38.0	38.0	36.0	38.0
78-79	37.170485478797104	38.0	38.0	38.0	36.0	38.0
80-81	37.15969423232437	38.0	38.0	38.0	36.0	38.0
82-83	37.160295192876376	38.0	38.0	38.0	36.0	38.0
84-85	37.1473790763352	38.0	38.0	38.0	36.0	38.0
86-87	37.1167284012256	38.0	38.0	38.0	36.0	38.0
88-89	37.0774176030597	38.0	38.0	38.0	36.0	38.0
90-91	37.08130371669846	38.0	38.0	38.0	36.0	38.0
92-93	37.05584491684809	38.0	38.0	38.0	36.0	38.0
94-95	37.041197235919284	38.0	38.0	38.0	36.0	38.0
96-97	37.030056865741315	38.0	38.0	38.0	36.0	38.0
98-99	37.069810004401944	38.0	38.0	38.0	36.0	38.0
100	35.44308585636222	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	6.0
19	22.0
20	55.0
21	159.0
22	507.0
23	1289.0
24	2847.0
25	5573.0
26	9555.0
27	15339.0
28	23474.0
29	34294.0
30	47933.0
31	64918.0
32	87127.0
33	116693.0
34	170019.0
35	299291.0
36	829762.0
37	5765623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16260224274804	19.676523792723813	14.121041820774149	25.039832143754005
2	14.23077504541781	20.49342222768079	42.46093814281133	22.814864584090074
3	17.978495124283377	25.693455246651574	30.2818078180842	26.046241810980842
4	12.36738394627808	17.71732683871049	39.06645950874782	30.848829706263608
5	13.410642858041259	37.241279095439225	35.55106151070021	13.797016535819312
6	30.103719520475323	37.590175109054336	18.824574535904482	13.481530834565861
7	26.530643098143564	32.56318926952732	23.169035368170317	17.737132264158795
8	24.236479577399873	36.70518479591046	21.20982451108029	17.848511115609377
9	25.55333868219254	16.93910662277873	21.43589443080472	36.071660264224015
10-11	24.250713042007753	26.59849880048664	28.923152544397844	20.227635613107758
12-13	25.018987915029932	24.197955419305476	29.025862764434397	21.7571939012302
14-15	22.172707144874483	25.486370911410557	26.69979133027252	25.641130613442442
16-17	21.814167680448072	28.540016972393172	27.402688691444006	22.24312665571475
18-19	21.83655716489591	27.76803129470622	29.122210462224118	21.273201078173752
20-21	22.5630250414414	26.890122431068136	29.0306726550992	21.516179872391266
22-23	22.351137637641084	26.83662433456876	28.8217412592086	21.99049676858155
24-25	22.150454734935927	26.93274640794423	28.922008959492153	21.99478989762769
26-27	22.07654630611805	27.074653496799623	28.862940131692426	21.985860065389904
28-29	21.98845826041828	27.128208189840215	28.914211471302064	21.96912207843944
30-31	22.310159457539775	26.89806706702743	28.846725291639103	21.94504818379369
32-33	22.06466142543329	27.079760998683593	28.76666954277586	22.088908033107263
34-35	22.18826348247258	27.107093789954263	28.830505443408672	21.87413728416449
36-37	22.2353351620081	27.03000816074849	28.71801199569177	22.016644681551636
38-39	22.04932527630285	27.146731248438833	28.817561482111948	21.986381993146374
40-41	22.262002503281476	26.97357351340013	28.737889090126284	22.026534893192114
42-43	22.196719781279228	27.067860644165737	28.555892914841447	22.179526659713584
44-45	22.199966900719247	27.17156363253351	28.491984540885046	22.136484925862195
46-47	22.267769302809988	27.13236856526556	28.29153019893425	22.3083319329902
48-49	22.253695937504798	27.2233074834552	28.249595481930847	22.273401097109165
50-51	22.219977700774525	27.333798833766625	28.150707156216765	22.295516309242082
52-53	22.39401119718895	27.271759219813408	28.032927917871465	22.30130166512618
54-55	22.33772371749799	27.228882300493645	28.022558407573595	22.41083557443477
56-57	22.354869194570355	27.348495861135625	27.983811031102395	22.31282391319163
58-59	22.29402118695709	27.355735082476514	28.076281367086654	22.27396236347975
60-61	22.423315388025994	27.34653606394961	28.02122116760782	22.208927380416572
62-63	22.365810975503543	27.401233021774296	27.995054218000025	22.237901784722133
64-65	22.358041404192775	27.31198456470212	27.953124796431556	22.37684923467355
66-67	22.378402401636347	27.3545456262502	27.852572109614616	22.41447986249884
68-69	22.317108074056787	27.305501465866193	27.951197722574804	22.42619273750222
70-71	22.362042842635063	27.176148394153994	27.922899670372857	22.538909092838086
72-73	22.458964875307565	27.163599289651213	27.83793576475023	22.53950007029099
74-75	22.329263597341146	27.304905638763998	27.843628588065716	22.522202175829143
76-77	22.43549823975664	27.26445836160376	27.71921634410599	22.580827054533607
78-79	22.361357335539907	27.237359610157373	27.738942130879103	22.662340923423617
80-81	22.39449910374199	27.327787146017428	27.73649686563802	22.541216884602562
82-83	22.466290838333563	27.28799871288415	27.79588946006633	22.44982098871596
84-85	22.585742662919152	27.28089235909621	27.665980335659096	22.46738464232554
86-87	22.419555507652113	27.33275956193914	27.723691653822414	22.523993276586335
88-89	22.509905781972076	27.25518079652559	27.677898625567416	22.557014795934915
90-91	22.52962786429149	27.384319571250625	27.619024856101614	22.467027708356273
92-93	22.60652044030685	27.264579710074127	27.674234624157908	22.454665225461117
94-95	22.469531171081993	27.383538252722	27.651373694358465	22.49555688183754
96-97	22.553093700153166	27.49021873834564	27.684369600789726	22.272317960711476
98-99	23.70182160829564	29.358220455482435	27.904374907897132	19.03558302832479
100	22.46328643956034	30.181872292447736	21.869684285659975	25.48515698233195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1664.0
1	1497.5
2	1911.0
3	2768.5
4	3483.0
5	4516.5
6	5443.0
7	6973.5
8	9164.5
9	10451.0
10	10542.5
11	10643.0
12	11744.0
13	13889.5
14	17781.0
15	22199.5
16	25120.0
17	26358.5
18	25715.0
19	23727.0
20	21975.5
21	21312.0
22	22870.5
23	27366.5
24	34347.5
25	44323.5
26	56734.5
27	70733.0
28	86609.0
29	104151.0
30	121140.5
31	138479.5
32	156718.0
33	173391.0
34	189267.0
35	205620.5
36	222892.0
37	238510.0
38	251713.5
39	262931.0
40	272912.0
41	281514.0
42	289029.0
43	297792.0
44	304010.5
45	310114.5
46	315289.0
47	317401.5
48	317611.5
49	313083.0
50	307684.0
51	300616.5
52	289008.5
53	274817.5
54	259858.5
55	243279.5
56	224861.0
57	204394.0
58	182469.5
59	159018.5
60	132776.5
61	107909.0
62	86285.0
63	67624.5
64	51912.0
65	38727.5
66	28720.0
67	21796.5
68	16491.0
69	12407.0
70	9408.0
71	6974.5
72	5032.5
73	3456.0
74	2328.0
75	1564.0
76	1018.5
77	612.5
78	369.0
79	215.5
80	133.5
81	82.5
82	57.5
83	44.0
84	32.0
85	21.5
86	13.0
87	9.5
88	5.0
89	3.0
90	3.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.012000817179390543
6	8.027302461130798E-5
7	2.6757674870435995E-5
8	2.6757674870435995E-5
9	0.0
10-11	2.0068256152826995E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.087937921765315E-4
26-27	0.0
28-29	6.626062069067527E-4
30-31	0.0023248979959379274
32-33	1.275782215649631E-4
34-35	6.733387353055605E-5
36-37	2.2311855783195832E-4
38-39	4.6936843755750185E-4
40-41	6.026356681835965E-4
42-43	1.861320842905348E-4
44-45	0.0
46-47	0.0014791891407607604
48-49	4.243751164821283E-5
50-51	0.0020542655647529115
52-53	0.0015961833100053302
54-55	0.002826969872474664
56-57	0.0027639438407558873
58-59	0.0038434149944676214
60-61	0.0030664196164519363
62-63	0.003130809030334386
64-65	0.0046127428194402045
66-67	0.0017357866600436593
68-69	1.4673313729271338E-4
70-71	6.630880196299017E-4
72-73	0.006571618268090192
74-75	0.00560790260862925
76-77	0.0043131712523361
78-79	0.0024825783442094873
80-81	2.4590939959990542E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0018010188303774005
90-91	3.106269625584065E-4
92-93	8.723299724204156E-6
94-95	5.293362097462971E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	179.0
22-23	361.0
24-25	614.0
26-27	1829.0
28-29	5116.0
30-31	15561.0
32-33	19211.0
34-35	26669.0
36-37	42070.0
38-39	49717.0
40-41	47753.0
42-43	53569.0
44-45	63146.0
46-47	64090.0
48-49	60524.0
50-51	56361.0
52-53	55201.0
54-55	59294.0
56-57	60436.0
58-59	59877.0
60-61	58987.0
62-63	57625.0
64-65	61265.0
66-67	64154.0
68-69	65119.0
70-71	65161.0
72-73	59938.0
74-75	60235.0
76-77	61626.0
78-79	63093.0
80-81	62276.0
82-83	59668.0
84-85	59437.0
86-87	60856.0
88-89	62827.0
90-91	63065.0
92-93	63131.0
94-95	73261.0
96-97	226301.0
98-99	492511.0
100-101	4892334.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.98554395315585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.72528858169504	74.51641623934385
2	7.766836748008922	13.046040181537787
3	1.9843772889642664	4.9997701806585795
4	0.7127762316471417	2.3945159812706325
5	0.3339686557640244	1.4024269608822924
6	0.1714134003879059	0.8637748603463024
7	0.09828883019082488	0.5778388607667057
8	0.0581115428278234	0.3904423627481489
9	0.03475807669414818	0.26272583801311894
>10	0.11232894323918823	1.3872719974810508
>50	0.0013933299410328582	0.07689756236336728
>100	4.0694073120321614E-4	0.05369514423823499
>500	5.1429908521677694E-5	0.028183830349996695
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.013651230565399E-5	0.0	0.0	0.0
3	0.0	4.013651230565399E-5	0.0	0.0	0.0
4	0.0	9.365186204652597E-5	0.0	0.0	1.3378837435217998E-5
5	0.0	9.365186204652597E-5	0.0	0.0	1.3378837435217998E-5
6	2.6757674870435995E-5	9.365186204652597E-5	0.0	0.0	1.3378837435217998E-5
7	2.6757674870435995E-5	9.365186204652597E-5	0.0	0.0	1.3378837435217998E-5
8	2.6757674870435995E-5	1.0703069948174398E-4	0.0	0.0	2.6757674870435995E-5
9	2.6757674870435995E-5	1.7392488665783396E-4	0.0	0.0	5.351534974087199E-5
10-11	2.6757674870435995E-5	1.7392488665783396E-4	0.0	2.0068256152826995E-5	5.351534974087199E-5
12-13	2.6757674870435995E-5	1.7392488665783396E-4	0.0	2.6757674870435995E-5	5.351534974087199E-5
14-15	2.6757674870435995E-5	2.3412965511631495E-4	0.0	2.6757674870435995E-5	7.358360589369899E-5
16-17	4.013651230565399E-5	2.4081907383392395E-4	0.0	2.6757674870435995E-5	9.365186204652597E-5
18-19	4.013651230565399E-5	2.4750849255153294E-4	0.0	3.3447093588044995E-5	1.0034128076413497E-4
20-21	4.013651230565399E-5	2.5419791126914197E-4	0.0	4.682593102326299E-5	1.0703069948174398E-4
22-23	4.682593102326299E-5	2.9433442357479594E-4	0.0	8.027302461130798E-5	1.4047779306978898E-4
24-25	5.351534974087199E-5	3.679180294684949E-4	0.0	1.1372011819935298E-4	1.6723546794022496E-4
26-27	5.351534974087199E-5	3.879862856213219E-4	0.0	1.5385663050500697E-4	1.7392488665783396E-4
28-29	5.351534974087199E-5	3.9467570433893086E-4	0.0	4.548804727974119E-4	1.8061430537544295E-4
30-31	5.351534974087199E-5	4.749487289502389E-4	0.0	0.0014716721178739797	1.8730372409305195E-4
32-33	5.351534974087199E-5	4.816381476678479E-4	0.0	0.003598907270073641	2.0068256152826994E-4
34-35	5.351534974087199E-5	5.418429161263288E-4	0.0	0.006495425574798337	2.0068256152826994E-4
36-37	5.351534974087199E-5	6.020476845848098E-4	0.0	0.010937199603290712	2.0068256152826994E-4
38-39	5.351534974087199E-5	6.288053594552458E-4	0.0	0.017472761690394703	2.2075081768109696E-4
40-41	5.351534974087199E-5	6.823207091961179E-4	0.0	0.024008323777498695	2.2744023639870595E-4
42-43	5.351534974087199E-5	7.023889653489448E-4	0.0	0.03083822028817748	2.3412965511631495E-4
44-45	5.351534974087199E-5	7.960408273954709E-4	0.0	0.03869828728136805	2.4750849255153294E-4
46-47	5.351534974087199E-5	8.562455958539518E-4	0.0	0.04753500940732955	2.6757674870435996E-4
48-49	5.351534974087199E-5	8.562455958539518E-4	0.0	0.055809820361011876	2.9433442357479594E-4
50-51	7.358360589369899E-5	9.900339702061318E-4	0.0	0.06398429003393007	2.9433442357479594E-4
52-53	8.027302461130798E-5	0.0010034128076413498	0.0	0.07228585866248283	3.144026797276229E-4
54-55	8.027302461130798E-5	0.0010167916450765679	0.0	0.08046701775411864	3.2109209844523193E-4
56-57	8.027302461130798E-5	0.0010368599012293947	0.0	0.08792571962425268	3.344709358804499E-4
58-59	8.027302461130798E-5	0.0010636175760998308	0.0	0.09561186173078541	3.478497733156679E-4
60-61	9.365186204652597E-5	0.0010836858322526579	0.0	0.10313745778809554	3.612286107508859E-4
62-63	1.0703069948174398E-4	0.0011171329258407028	0.0	0.10983356592442214	3.746074481861039E-4
64-65	1.0703069948174398E-4	0.0012509213001928826	0.0	0.11645609045485505	3.746074481861039E-4
66-67	1.0703069948174398E-4	0.0012576107189104916	0.0	0.12279765939914838	3.746074481861039E-4
68-69	1.0703069948174398E-4	0.0012643001376281006	0.0	0.1291325389247241	3.746074481861039E-4
70-71	1.0703069948174398E-4	0.0013111260686513636	0.0	0.13420311831267173	3.879862856213219E-4
72-73	1.0703069948174398E-4	0.0013178154873689726	0.0	0.13826359547426037	4.548804727974119E-4
74-75	1.0703069948174398E-4	0.0013311943248041907	0.0	0.14126714447846683	4.548804727974119E-4
76-77	1.1372011819935298E-4	0.0013646414183922356	0.0	0.14452489139394242	4.548804727974119E-4
78-79	1.2040953691696197E-4	0.0013847096745450626	0.0	0.14657185352153074	4.548804727974119E-4
80-81	1.2040953691696197E-4	0.0013913990932626717	0.0	0.14730768958046775	4.548804727974119E-4
82-83	1.2040953691696197E-4	0.0014114673494154987	0.0	0.1478696007527469	4.548804727974119E-4
84-85	1.2040953691696197E-4	0.0014315356055683256	0.0	0.14807697273299278	4.548804727974119E-4
86-87	1.2040953691696197E-4	0.0014449144430035436	0.0	0.1482308293634978	4.615698915150209E-4
88	1.2040953691696197E-4	0.0014716721178739797	0.0	0.14823751878221542	4.682593102326299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8330	0.0	49.584694	1
GTATCAA	15885	0.0	38.386337	1
TCAACGC	18935	0.0	31.640959	4
ATCAACG	18920	0.0	31.576021	3
CAACGCA	19155	0.0	31.233097	5
TATCAAC	19860	0.0	30.553186	2
AACGCAG	19835	0.0	30.20204	6
ACGCAGA	22920	0.0	26.02543	7
CGCAGAG	23760	0.0	25.087421	8
GCAGAGT	27860	0.0	21.334316	9
GTACATG	20225	0.0	21.26443	1
TACATGG	20375	0.0	20.543612	2
ACATGGG	20535	0.0	19.740726	3
GAGTACT	16410	0.0	19.485239	12-13
GTGGTAT	4470	0.0	19.337904	1
AGAGTAC	24790	0.0	18.669273	10-11
CAGAGTA	27235	0.0	18.267359	10-11
CATGGGG	16260	0.0	18.095825	4
TGGTATC	4530	0.0	17.67179	2
GTACTTT	18580	0.0	17.255344	14-15
>>END_MODULE