FastQCFastQC Report
Fri 27 May 2016
SRR2138867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4979980
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.047.5196531
GTATCAA100750.038.8009761
TCAACGC118950.032.2876474
ATCAACG118800.032.2562523
CAACGCA120550.031.8591085
AACGCAG124800.030.9427876
TATCAAC128400.030.345322
ACGCAGA142700.026.9710127
CGCAGAG144850.026.5706848
GCAGAGT173800.022.1447839
AGAGTAC155500.019.4451610-11
TACATGG124850.019.4321462
GAGTACT103150.019.42483112-13
GTACATG128600.019.3654691
CTTATAC30950.019.2507211
CAGAGTA169150.018.67413110-11
ACATGGG126700.018.5056173
GTGGTAT27950.018.0964361
AGTACTT114250.016.97490112-13
GTACTTT119550.016.9214414-15
Produced by FastQC (version 0.11.5)