##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4979980
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.27271314342628	32.0	30.0	33.0	18.0	34.0
2	32.01219884417206	33.0	32.0	33.0	30.0	34.0
3	32.12423363146037	33.0	33.0	33.0	29.0	34.0
4	32.6566652878124	33.0	33.0	34.0	31.0	34.0
5	32.68770015140623	33.0	33.0	34.0	32.0	34.0
6	36.035561186992716	38.0	36.0	38.0	33.0	38.0
7	37.0682295912835	38.0	38.0	38.0	36.0	38.0
8	36.930155944401385	38.0	38.0	38.0	35.0	38.0
9	37.26718179591083	38.0	38.0	38.0	36.0	38.0
10-11	37.37896708420516	38.0	38.0	38.0	37.0	38.0
12-13	37.41401039763212	38.0	38.0	38.0	37.0	38.0
14-15	37.44633422222579	38.0	38.0	38.0	37.0	38.0
16-17	37.45345121867959	38.0	38.0	38.0	37.0	38.0
18-19	37.46913471540046	38.0	38.0	38.0	37.0	38.0
20-21	37.4789467407325	38.0	38.0	38.0	37.0	38.0
22-23	37.49729930008445	38.0	38.0	38.0	37.0	38.0
24-25	37.46153329522539	38.0	38.0	38.0	37.0	38.0
26-27	37.47439166566433	38.0	38.0	38.0	37.0	38.0
28-29	37.4664968864099	38.0	38.0	38.0	37.0	38.0
30-31	37.45976071329225	38.0	38.0	38.0	37.0	38.0
32-33	37.45628986210269	38.0	38.0	38.0	37.0	38.0
34-35	37.44536031788761	38.0	38.0	38.0	37.0	38.0
36-37	37.42582993347671	38.0	38.0	38.0	37.0	38.0
38-39	37.40150886646248	38.0	38.0	38.0	37.0	38.0
40-41	37.38881761017244	38.0	38.0	38.0	37.0	38.0
42-43	37.372319069853056	38.0	38.0	38.0	37.0	38.0
44-45	37.34785508056276	38.0	38.0	38.0	37.0	38.0
46-47	37.3199200824417	38.0	38.0	38.0	37.0	38.0
48-49	37.28339816749542	38.0	38.0	38.0	37.0	38.0
50-51	37.26593872164139	38.0	38.0	38.0	37.0	38.0
52-53	37.254661526700986	38.0	38.0	38.0	37.0	38.0
54-55	37.23214549712014	38.0	38.0	38.0	37.0	38.0
56-57	37.201146669046224	38.0	38.0	38.0	37.0	38.0
58-59	37.20317513377302	38.0	38.0	38.0	37.0	38.0
60-61	37.21679196592507	38.0	38.0	38.0	37.0	38.0
62-63	37.22176274762509	38.0	38.0	38.0	37.0	38.0
64-65	37.225890029620395	38.0	38.0	38.0	37.0	38.0
66-67	37.22859158324246	38.0	38.0	38.0	37.0	38.0
68-69	37.225433731874915	38.0	38.0	38.0	37.0	38.0
70-71	37.19101419622408	38.0	38.0	38.0	36.5	38.0
72-73	37.1668803530097	38.0	38.0	38.0	36.0	38.0
74-75	37.151680767165914	38.0	38.0	38.0	36.0	38.0
76-77	37.15207856254313	38.0	38.0	38.0	36.0	38.0
78-79	37.15220911682137	38.0	38.0	38.0	36.0	38.0
80-81	37.141028083725445	38.0	38.0	38.0	36.0	38.0
82-83	37.13831158224794	38.0	38.0	38.0	36.0	38.0
84-85	37.12532476603168	38.0	38.0	38.0	36.0	38.0
86-87	37.09472124468985	38.0	38.0	38.0	36.0	38.0
88-89	37.05735260134535	38.0	38.0	38.0	36.0	38.0
90-91	37.05833759250754	38.0	38.0	38.0	36.0	38.0
92-93	37.03315843703958	38.0	38.0	38.0	36.0	38.0
94-95	37.01846782490074	38.0	38.0	38.0	36.0	38.0
96-97	37.01077774040234	38.0	38.0	38.0	36.0	38.0
98-99	37.05275526957068	38.0	38.0	38.0	36.0	38.0
100	35.403788047745934	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	3.0
19	13.0
20	48.0
21	127.0
22	313.0
23	894.0
24	1920.0
25	3648.0
26	6275.0
27	10495.0
28	15869.0
29	22934.0
30	32697.0
31	44202.0
32	59265.0
33	79662.0
34	117593.0
35	208253.0
36	573818.0
37	3801946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.980385463395436	19.693753790175865	14.008409672328002	25.3174510741007
2	14.139855983357364	20.44154795802393	42.79890682291897	22.61968923569974
3	18.018787224045077	25.69136823842666	30.200462652460452	26.089381885067812
4	12.354868895055803	17.695994763031177	39.10624139052767	30.842894951385347
5	13.370588104165817	37.18370480661653	35.66707842921858	13.778628659999073
6	29.966080144193523	37.734574517858924	18.857708895668935	13.441636442278615
7	26.494408912166094	32.39365065595658	23.285178511796936	17.826761920080386
8	23.96919264736003	37.07464688613207	21.312756276129623	17.643404190378277
9	25.341969244856404	17.102859850842776	21.505869501483943	36.04930140281688
10-11	24.219353981081845	26.698812725678707	28.97309988116413	20.108733412075313
12-13	24.96160627151113	24.152416274764153	29.10804862670131	21.777928827023402
14-15	22.09792007196816	25.481578239270036	26.71207514889618	25.70842653986562
16-17	21.82275832433062	28.654301824505318	27.309959076140867	22.21298077502319
18-19	21.830890887111998	27.778635657171314	29.060287390712414	21.330186065004277
20-21	22.5204028480653	27.035033741356827	28.91435281776374	21.530210592814132
22-23	22.286144997779527	26.952462988676135	28.7808219356887	21.98057007785564
24-25	22.120005108958992	27.067963613929784	28.83784437275479	21.974186904356433
26-27	22.07336146753061	27.17269392888793	28.80021288934524	21.95373171423622
28-29	21.97516709970677	27.182737565444437	28.878409230278123	21.96368610457067
30-31	22.23558074137402	27.132995490921726	28.728947011795665	21.90247675590859
32-33	22.146517961581196	27.112862502388417	28.745479878490432	21.99513965753995
34-35	22.18287704631407	27.1517493327684	28.772553367168076	21.892820253749456
36-37	22.11692042985294	27.17444372291235	28.733023992355434	21.975611854879276
38-39	22.001951666942684	27.17555065798693	28.863971871536492	21.95852580353389
40-41	22.209780047970444	27.034908997247115	28.74466607715301	22.010644877629435
42-43	22.207111411424577	27.10658348442138	28.583489579437927	22.102815524716114
44-45	22.20663098981698	27.236287522644375	28.521672361420585	22.03540912611806
46-47	22.256915381415034	27.177431411949065	28.279328615077652	22.286324591558245
48-49	22.218237432171716	27.279298735167778	28.2679374707919	22.23452636186861
50-51	22.169956557345284	27.35334963266874	28.229602159654842	22.247091650331132
52-53	22.365684910928913	27.294678710067156	28.1338882441911	22.205748134812833
54-55	22.28838528167298	27.224279912101974	28.156167585467973	22.331167220757074
56-57	22.308072652928637	27.374664199106768	28.07694708417362	22.240316063790978
58-59	22.210791482436605	27.46572810114469	28.17623169150271	22.14724872491599
60-61	22.33476664660848	27.470117339856507	28.066059338793014	22.129056674742
62-63	22.266723440617564	27.610264471243447	27.99366731469821	22.129344773440778
64-65	22.21950853696107	27.56847494941961	27.977498256935775	22.234518256683547
66-67	22.290995573597545	27.56479018497913	27.863488848560504	22.280725392862816
68-69	22.244192899873696	27.520435654797108	27.963744826501795	22.271626618827398
70-71	22.249690847678412	27.28266053280049	27.975870484691086	22.491778134830017
72-73	22.38189876540697	27.249777529463966	27.959483982998773	22.408839722130296
74-75	22.232861467777624	27.407358798832455	27.921898049799932	22.437881683589993
76-77	22.381054294176657	27.38196002881677	27.815354947341742	22.42163072966483
78-79	22.339444991768108	27.348079615255845	27.76363552211371	22.548839870862338
80-81	22.347776887731438	27.376094968416574	27.783170792312674	22.49295735153932
82-83	22.373439164631144	27.33254710708899	27.87203083268369	22.421982895596173
84-85	22.54657058260866	27.355407894836127	27.701887908501867	22.39613361405335
86-87	22.352455423276147	27.402788201303146	27.79225591752084	22.45250045789987
88-89	22.451869568545003	27.32667667044803	27.765032434115188	22.456421326891775
90-91	22.4606563153069	27.477933067139983	27.686227283739985	22.375183333813137
92-93	22.498161957980294	27.318986160087317	27.810717898739295	22.372133983193095
94-95	22.384660406315845	27.49110371198918	27.7396144941862	22.38462138750878
96-97	22.499136710122773	27.600239014099852	27.770005050988573	22.1306192247888
98-99	23.606969477058204	29.460704462896985	27.97289625627971	18.959429803765097
100	22.40578864789588	30.327436875151054	21.918475318156222	25.348299158796845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	824.0
1	789.0
2	1197.5
3	1791.5
4	2219.0
5	2931.5
6	3656.5
7	4672.0
8	5965.0
9	6689.5
10	6677.5
11	6691.0
12	7329.5
13	8694.0
14	11161.0
15	13882.0
16	15812.0
17	16749.0
18	16349.0
19	15080.0
20	13954.0
21	13681.0
22	14797.0
23	17551.5
24	22145.0
25	28749.0
26	37231.0
27	46997.0
28	57489.5
29	69083.5
30	80330.5
31	91398.0
32	103526.5
33	114675.5
34	125205.0
35	136193.5
36	147971.5
37	158803.5
38	167250.5
39	174629.5
40	182798.5
41	190023.0
42	194316.0
43	201293.0
44	206520.5
45	208332.0
46	210123.5
47	209522.0
48	208195.0
49	205694.5
50	202171.0
51	197112.5
52	190217.0
53	181904.5
54	172139.5
55	160269.0
56	147066.5
57	132903.0
58	117941.5
59	102779.5
60	86058.5
61	69915.5
62	55682.5
63	43010.0
64	32542.5
65	24164.5
66	17705.0
67	13297.5
68	10084.0
69	7480.5
70	5506.0
71	3964.5
72	2764.0
73	1842.0
74	1185.0
75	732.0
76	425.5
77	249.0
78	162.5
79	109.0
80	71.0
81	43.0
82	26.5
83	16.5
84	12.0
85	7.0
86	4.5
87	4.5
88	3.5
89	3.0
90	3.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.011506070305503236
6	1.204824115759501E-4
7	2.0080401929325018E-5
8	0.0
9	0.0
10-11	4.0160803858650036E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.823858016751423E-4
26-27	0.0
28-29	5.524507821598174E-4
30-31	0.002201610634000529
32-33	1.813031161473088E-4
34-35	1.0097702338516787E-4
36-37	2.8379205785628145E-4
38-39	6.318672891170949E-4
40-41	5.947052773941244E-4
42-43	1.4442535164220394E-4
44-45	0.0
46-47	0.0011734210493506319
48-49	6.338100923271161E-5
50-51	0.001639559635574867
52-53	0.0013514694817275425
54-55	0.0024745697436410583
56-57	0.0022330853047300885
58-59	0.0032621578811922103
60-61	0.0027134860032854225
62-63	0.0027693843223143477
64-65	0.004121028527651088
66-67	0.0013746402503981062
68-69	9.175148422361902E-5
70-71	4.979765360404728E-4
72-73	0.006094391241669634
74-75	0.005346220281270196
76-77	0.004263500021496138
78-79	0.0024042926240509807
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0019795343882948744
90-91	1.7833377152488622E-4
92-93	0.0
94-95	1.4306875455137474E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	21.0
20-21	110.0
22-23	273.0
24-25	315.0
26-27	970.0
28-29	2485.0
30-31	9147.0
32-33	11530.0
34-35	16017.0
36-37	25471.0
38-39	30077.0
40-41	28991.0
42-43	33044.0
44-45	39035.0
46-47	39723.0
48-49	37554.0
50-51	35345.0
52-53	33846.0
54-55	36538.0
56-57	37765.0
58-59	37829.0
60-61	37271.0
62-63	36673.0
64-65	38502.0
66-67	41084.0
68-69	42371.0
70-71	43617.0
72-73	38398.0
74-75	37853.0
76-77	38886.0
78-79	40188.0
80-81	39704.0
82-83	37862.0
84-85	37192.0
86-87	38853.0
88-89	40020.0
90-91	40313.0
92-93	39872.0
94-95	47325.0
96-97	151960.0
98-99	333447.0
100-101	3322503.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.0490337569399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8136049765617	79.05236565194126
2	6.706417384626164	11.675743066049032
3	1.4476269950322942	3.78043593474071
4	0.49205465162767126	1.7133152791918584
5	0.21274287196762473	0.9259530721729053
6	0.10184223796211635	0.5319161046147942
7	0.06547458462102289	0.3989649528827928
8	0.041555405700745895	0.289388633090205
9	0.027651930875011117	0.21663664777650704
>10	0.08904330070027011	1.1987311452679157
>50	0.0012145271925895677	0.06794221893409237
>100	7.020409487169429E-4	0.1127012783643252
>500	6.909218412671023E-5	0.03590601497359142
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.0080401929325018E-5	0.0
4	0.0	1.0040200964662508E-4	0.0	4.0160803858650036E-5	2.0080401929325018E-5
5	0.0	1.0040200964662508E-4	0.0	4.0160803858650036E-5	2.0080401929325018E-5
6	0.0	1.0040200964662508E-4	0.0	4.0160803858650036E-5	2.0080401929325018E-5
7	0.0	1.0040200964662508E-4	0.0	4.0160803858650036E-5	2.0080401929325018E-5
8	0.0	1.0040200964662508E-4	0.0	4.0160803858650036E-5	2.0080401929325018E-5
9	0.0	1.8072361736392516E-4	0.0	1.4056281350527513E-4	2.0080401929325018E-5
10-11	0.0	1.8072361736392516E-4	0.0	1.5060301446993764E-4	2.0080401929325018E-5
12-13	0.0	1.8072361736392516E-4	0.0	2.0080401929325017E-4	2.0080401929325018E-5
14-15	0.0	2.1084422025791267E-4	0.0	2.0080401929325017E-4	3.0120602893987527E-5
16-17	0.0	2.2088442122257518E-4	0.0	2.2088442122257518E-4	4.0160803858650036E-5
18-19	0.0	2.6104522508122526E-4	0.0	2.3092462218723768E-4	4.0160803858650036E-5
20-21	0.0	3.012060289398753E-4	0.0	2.610452250812252E-4	5.020100482331254E-5
22-23	2.0080401929325018E-5	3.313266318338628E-4	0.0	2.8112562701055027E-4	7.028140675263757E-5
24-25	2.0080401929325018E-5	3.413668327985253E-4	0.0	2.911658279752128E-4	8.032160771730007E-5
26-27	2.0080401929325018E-5	3.413668327985253E-4	0.0	3.413668327985253E-4	1.0040200964662508E-4
28-29	2.0080401929325018E-5	3.413668327985253E-4	0.0	4.1164823955116284E-4	1.0040200964662508E-4
30-31	2.0080401929325018E-5	3.614472347278503E-4	0.0	0.0010943819051482135	1.0040200964662508E-4
32-33	2.0080401929325018E-5	3.614472347278503E-4	0.0	0.0028714974758934776	1.2048241157595011E-4
34-35	2.0080401929325018E-5	4.0160803858650034E-4	0.0	0.004919698472684629	1.2048241157595011E-4
36-37	2.0080401929325018E-5	4.4176884244515036E-4	0.0	0.008142602982341294	1.2048241157595011E-4
38-39	2.0080401929325018E-5	4.4176884244515036E-4	0.0	0.013072341655990586	1.2048241157595011E-4
40-41	2.0080401929325018E-5	5.421708520917754E-4	0.0	0.01884545721067153	1.4056281350527513E-4
42-43	2.0080401929325018E-5	5.421708520917754E-4	0.0	0.024478009951847197	1.4056281350527513E-4
44-45	2.0080401929325018E-5	6.224924598090755E-4	0.0	0.03086357776537255	1.5060301446993764E-4
46-47	2.0080401929325018E-5	7.028140675263756E-4	0.0	0.03781139683291901	1.6064321543460015E-4
48-49	2.0080401929325018E-5	7.028140675263756E-4	0.0	0.044648773689854176	2.0080401929325017E-4
50-51	2.0080401929325018E-5	8.132562781376631E-4	0.0	0.050873698287944934	2.0080401929325017E-4
52-53	2.0080401929325018E-5	8.232964791023257E-4	0.0	0.057239185699540965	2.1084422025791267E-4
54-55	2.0080401929325018E-5	8.232964791023257E-4	0.0	0.06350427110149037	2.2088442122257518E-4
56-57	2.0080401929325018E-5	8.232964791023257E-4	0.0	0.06844404997610432	2.2088442122257518E-4
58-59	2.0080401929325018E-5	8.232964791023257E-4	0.0	0.07430752733946723	2.2088442122257518E-4
60-61	2.0080401929325018E-5	8.634572829609757E-4	0.0	0.07952843184109173	2.2088442122257518E-4
62-63	2.0080401929325018E-5	8.734974839256383E-4	0.0	0.08472925594078691	2.2088442122257518E-4
64-65	2.0080401929325018E-5	0.0010542211012895633	0.0	0.08977947702601216	2.2088442122257518E-4
66-67	2.0080401929325018E-5	0.0010843417041835508	0.0	0.09500038152763665	2.2088442122257518E-4
68-69	2.0080401929325018E-5	0.001124502508042201	0.0	0.09960883377041675	2.2088442122257518E-4
70-71	2.0080401929325018E-5	0.001124502508042201	0.0	0.10387591918039832	2.610452250812252E-4
72-73	2.0080401929325018E-5	0.001124502508042201	0.0	0.10740002971899486	3.212864308692003E-4
74-75	2.0080401929325018E-5	0.0011646633119008509	0.0	0.10999040156787779	3.212864308692003E-4
76-77	2.0080401929325018E-5	0.001265065321547476	0.0	0.11256069301483138	3.212864308692003E-4
78-79	2.0080401929325018E-5	0.001305226125406126	0.0	0.11454865280583457	3.212864308692003E-4
80-81	2.0080401929325018E-5	0.001305226125406126	0.0	0.11504062265310303	3.413668327985253E-4
82-83	2.0080401929325018E-5	0.001305226125406126	0.0	0.11553259250037148	3.614472347278503E-4
84-85	2.0080401929325018E-5	0.001305226125406126	0.0	0.11572335631870007	3.614472347278503E-4
86-87	2.0080401929325018E-5	0.001305226125406126	0.0	0.11588399953413467	3.614472347278503E-4
88	2.0080401929325018E-5	0.001345386929264776	0.0	0.11588399953413467	3.614472347278503E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5340	0.0	47.519653	1
GTATCAA	10075	0.0	38.800976	1
TCAACGC	11895	0.0	32.287647	4
ATCAACG	11880	0.0	32.256252	3
CAACGCA	12055	0.0	31.859108	5
AACGCAG	12480	0.0	30.942787	6
TATCAAC	12840	0.0	30.34532	2
ACGCAGA	14270	0.0	26.971012	7
CGCAGAG	14485	0.0	26.570684	8
GCAGAGT	17380	0.0	22.144783	9
AGAGTAC	15550	0.0	19.44516	10-11
TACATGG	12485	0.0	19.432146	2
GAGTACT	10315	0.0	19.424831	12-13
GTACATG	12860	0.0	19.365469	1
CTTATAC	3095	0.0	19.250721	1
CAGAGTA	16915	0.0	18.674131	10-11
ACATGGG	12670	0.0	18.505617	3
GTGGTAT	2795	0.0	18.096436	1
AGTACTT	11425	0.0	16.974901	12-13
GTACTTT	11955	0.0	16.92144	14-15
>>END_MODULE