FastQCFastQC Report
Fri 27 May 2016
SRR2138866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263681
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81400.052.1346861
GTATCAA154500.037.42411
ATCAACG190000.030.365883
TCAACGC191950.030.0791154
CAACGCA194850.029.5886525
TATCAAC199600.029.302222
AACGCAG201700.028.6854866
ACGCAGA229850.025.1542057
CGCAGAG232700.024.8461278
CCGAACC8450.023.1856789
GGACCGA10200.022.4771466
GTACATG223750.022.1152081
ACCGAAC8900.022.0133678
TGGTATC38100.021.9924932
TACATGG224900.021.7982312
GTGGTAT39300.021.7452641
GCAGAGT268500.021.5488369
ACATGGG228150.021.158823
GAGTACT136200.020.2915412-13
AGGACCG12750.019.2905775
Produced by FastQC (version 0.11.5)