##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5263681
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.081326546954497	32.0	27.0	33.0	18.0	33.0
2	31.34997941554589	33.0	32.0	33.0	27.0	33.0
3	32.2275899698329	33.0	33.0	33.0	30.0	34.0
4	32.38819202759438	33.0	33.0	33.0	31.0	34.0
5	33.07545841018861	33.0	33.0	34.0	33.0	34.0
6	37.102062985959826	38.0	37.0	38.0	36.0	38.0
7	37.252365597383275	38.0	38.0	38.0	36.0	38.0
8	37.31984271083297	38.0	38.0	38.0	37.0	38.0
9	37.46675396932299	38.0	38.0	38.0	37.0	38.0
10-11	37.531223586687716	38.0	38.0	38.0	37.5	38.0
12-13	37.560705711459335	38.0	38.0	38.0	38.0	38.0
14-15	37.5605193399828	38.0	38.0	38.0	38.0	38.0
16-17	37.55828126742483	38.0	38.0	38.0	38.0	38.0
18-19	37.55704857494214	38.0	38.0	38.0	38.0	38.0
20-21	37.554335405328736	38.0	38.0	38.0	38.0	38.0
22-23	37.56374265355693	38.0	38.0	38.0	38.0	38.0
24-25	37.52329201379698	38.0	38.0	38.0	38.0	38.0
26-27	37.529016907914894	38.0	38.0	38.0	38.0	38.0
28-29	37.52168388857399	38.0	38.0	38.0	38.0	38.0
30-31	37.51241548950698	38.0	38.0	38.0	38.0	38.0
32-33	37.50960053368573	38.0	38.0	38.0	38.0	38.0
34-35	37.493959209500176	38.0	38.0	38.0	38.0	38.0
36-37	37.47290887713787	38.0	38.0	38.0	37.5	38.0
38-39	37.45165873905173	38.0	38.0	38.0	37.0	38.0
40-41	37.43376136695709	38.0	38.0	38.0	37.0	38.0
42-43	37.41521898838698	38.0	38.0	38.0	37.0	38.0
44-45	37.3832696533934	38.0	38.0	38.0	37.0	38.0
46-47	37.35075438907519	38.0	38.0	38.0	37.0	38.0
48-49	37.30980356608865	38.0	38.0	38.0	37.0	38.0
50-51	37.29432722113298	38.0	38.0	38.0	37.0	38.0
52-53	37.2866060399065	38.0	38.0	38.0	37.0	38.0
54-55	37.266480510325096	38.0	38.0	38.0	37.0	38.0
56-57	37.23634303711586	38.0	38.0	38.0	37.0	38.0
58-59	37.24031302137429	38.0	38.0	38.0	37.0	38.0
60-61	37.25449151012883	38.0	38.0	38.0	37.0	38.0
62-63	37.2593456086047	38.0	38.0	38.0	37.0	38.0
64-65	37.267115498001814	38.0	38.0	38.0	37.0	38.0
66-67	37.272344417802174	38.0	38.0	38.0	37.0	38.0
68-69	37.27649583512774	38.0	38.0	38.0	37.0	38.0
70-71	37.2448086292474	38.0	38.0	38.0	37.0	38.0
72-73	37.22776315268737	38.0	38.0	38.0	37.0	38.0
74-75	37.212136508317336	38.0	38.0	38.0	37.0	38.0
76-77	37.21621365467297	38.0	38.0	38.0	37.0	38.0
78-79	37.22027984271918	38.0	38.0	38.0	37.0	38.0
80-81	37.20763258536306	38.0	38.0	38.0	36.5	38.0
82-83	37.207097762671054	38.0	38.0	38.0	36.5	38.0
84-85	37.19934169941244	38.0	38.0	38.0	36.0	38.0
86-87	37.17217675986065	38.0	38.0	38.0	36.0	38.0
88-89	37.1308699921825	38.0	38.0	38.0	36.0	38.0
90-91	37.13541652731212	38.0	38.0	38.0	36.0	38.0
92-93	37.11428795421924	38.0	38.0	38.0	36.0	38.0
94-95	37.096097648567365	38.0	38.0	38.0	36.0	38.0
96-97	37.083455032810576	38.0	38.0	38.0	36.0	38.0
98-99	37.12207126240132	38.0	38.0	38.0	36.0	38.0
100	35.527602248513574	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	22.0
20	46.0
21	132.0
22	345.0
23	860.0
24	1850.0
25	3584.0
26	6139.0
27	10159.0
28	15542.0
29	23101.0
30	31952.0
31	42731.0
32	54547.0
33	72904.0
34	106261.0
35	192242.0
36	598318.0
37	4102944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.024731551931055	20.251094243743115	14.245506139144831	24.478668065181004
2	13.836115828447811	20.992153589854702	43.51745480016741	21.654275781530075
3	17.67711607143366	27.426149115039454	30.56290835253884	24.333826460988043
4	11.979050402180528	18.539915317816565	39.578139328732114	29.902894951270792
5	12.770025502012686	38.283419283716825	35.69736471602173	13.249190498248756
6	28.68521097688101	38.50919157144971	19.486306256021212	13.319291195648065
7	25.743914095065445	33.39821824241182	23.712046270298778	17.145821392223954
8	23.8900921028634	36.98978661316797	21.70323803878655	17.416883245182078
9	25.800176720435758	17.02046533595026	21.87896644952458	35.3003914940894
10-11	23.86313831067586	27.276786825336423	29.062372534495363	19.797702329492356
12-13	24.808342298858914	24.980408197229277	29.36704370952571	20.844205794386095
14-15	21.94086229769623	26.12466446959837	27.38820988581945	24.54626334688595
16-17	21.00799801507728	29.318722012219204	28.052896822584806	21.62038315011871
18-19	21.065115838136848	28.323068970175054	30.140304855100453	20.47151033658765
20-21	22.149001202152853	27.356958823129222	29.879902535542946	20.614137439174982
22-23	21.890091515950708	27.23307150706581	29.75122861157055	21.12560836541293
24-25	21.543194588964877	27.391088372349493	29.870642263325443	21.195074775360187
26-27	21.363607142558262	27.619793279373354	29.753825039123395	21.26277453894499
28-29	21.25631426544426	27.685066210344587	29.791124936438678	21.267494587772475
30-31	21.59579452097475	27.532626392044946	29.7104474105027	21.161131676477602
32-33	21.39159229997186	27.617872465238612	29.611630774316488	21.378904460473034
34-35	21.529809440405568	27.69967415204844	29.736334107956473	21.034182299589514
36-37	21.43215068814982	27.656894709829565	29.62655987918182	21.284394722838794
38-39	21.324677150368153	27.681915469307416	29.66703125130457	21.326376129019856
40-41	21.481380971792934	27.59230803269515	29.60648303154973	21.319827963962183
42-43	21.514663264761207	27.604719591052746	29.342974434479117	21.53764270970693
44-45	21.49591583459078	27.77356055131624	29.254270452543444	21.47625316154954
46-47	21.547589093166657	27.827141163810587	28.99372085100088	21.631548892021872
48-49	21.60631877775788	27.967850724417115	28.806615174202182	21.619215323622825
50-51	21.509144854889044	28.11239581363963	28.619681617079934	21.758777714391393
52-53	21.67124763130376	28.073504861631456	28.47085069196	21.784396815104788
54-55	21.640023259160397	28.056025628834625	28.468677972548466	21.835273139456508
56-57	21.776875522387108	28.050406174230634	28.344493522134346	21.82822478124791
58-59	21.663881070849055	28.14295841541962	28.476403730527426	21.71675678320389
60-61	21.78947533914537	28.15449512974076	28.40791016625618	21.648119364857692
62-63	21.696726410775522	28.193141008373406	28.339714234790808	21.770418346060264
64-65	21.577261180279038	28.15627553952312	28.439351961302563	21.827111318895284
66-67	21.677891926429137	28.216218756608992	28.25725184604143	21.848637470920444
68-69	21.656923019622276	28.126922771696165	28.35422233447451	21.86193187420705
70-71	21.68676921187391	27.95899016618226	28.34786545936825	22.00637516257558
72-73	21.788809730354696	28.015303622563824	28.334531030323184	21.861355616758296
74-75	21.737991398435796	28.050392211494717	28.27474414912066	21.936872240948823
76-77	21.789601511998736	27.969439987957113	28.146930337941672	22.09402816210248
78-79	21.7686669376126	28.016473097023546	28.175311053167	22.03954891219685
80-81	21.777760082213163	28.081934456464886	28.13499282662003	22.00531263470192
82-83	21.82221054703521	28.03614726364443	28.242516296592452	21.89912589272791
84-85	21.989767339775547	28.065370509819733	28.061331334542267	21.88353081586245
86-87	21.782481298889902	28.154511196529107	28.13796732661119	21.9250401779698
88-89	21.923604176891704	28.068334561079872	28.080652322158826	21.927408939869604
90-91	22.01993394022396	28.024606460968336	28.103736405381458	21.85172319342625
92-93	21.975737215671312	28.096679261858558	28.041589986939965	21.885993535530165
94-95	21.787256919438878	28.233538304750045	28.075179762278307	21.90402501353277
96-97	21.95750687600036	28.299340170302322	28.111034542965065	21.63211841073225
98-99	22.918772261216866	30.240795792760704	28.353050411128226	18.487381534894205
100	28.988780256381414	41.25004279544754	29.761176948171048	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1747.0
1	1439.5
2	1354.5
3	2012.5
4	3133.5
5	4355.0
6	5092.0
7	5238.5
8	4893.0
9	4688.5
10	4877.5
11	5730.0
12	7476.5
13	11238.0
14	16963.0
15	22988.5
16	27807.5
17	29657.5
18	29124.5
19	26377.0
20	23598.0
21	22119.5
22	22435.5
23	25887.5
24	31893.5
25	40517.5
26	51489.0
27	63707.0
28	76870.5
29	91560.5
30	105834.0
31	119323.0
32	133810.0
33	148511.5
34	161192.0
35	172911.0
36	185288.5
37	195947.5
38	204209.0
39	209761.0
40	214269.0
41	217680.5
42	218740.0
43	220685.0
44	222691.0
45	223548.0
46	223384.0
47	222045.0
48	218170.0
49	211655.0
50	204980.5
51	196588.5
52	187039.5
53	176924.0
54	165034.0
55	150816.5
56	134645.0
57	118493.5
58	103532.0
59	89037.5
60	74963.0
61	61361.0
62	48417.5
63	37744.0
64	29118.5
65	21922.0
66	15839.5
67	11545.0
68	8548.5
69	6189.0
70	4427.0
71	3124.0
72	2217.0
73	1520.0
74	1016.0
75	665.0
76	418.0
77	248.5
78	173.5
79	118.0
80	72.0
81	51.5
82	37.0
83	29.5
84	27.0
85	21.0
86	12.0
87	7.0
88	6.0
89	4.5
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.010771929377939127
6	0.0
7	3.799622355534084E-5
8	1.899811177767042E-5
9	0.0
10-11	3.799622355534084E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.038713481448693E-4
26-27	0.0
28-29	6.089670396589784E-4
30-31	0.002248583368658006
32-33	2.3885182970055625E-4
34-35	4.79385328296975E-5
36-37	3.18002435898659E-4
38-39	5.533785556877947E-4
40-41	6.265499769547087E-4
42-43	2.270300832627895E-4
44-45	0.0
46-47	0.0014107304389376918
48-49	3.056052588552944E-5
50-51	0.001907053117408189
52-53	0.0013132470460540105
54-55	0.002482374092092713
56-57	0.002304556852302438
58-59	0.0036050322387518436
60-61	0.002623858878894492
62-63	0.00282622000413855
64-65	0.004050741850206222
66-67	0.0016672561741349486
68-69	6.792491534574405E-5
70-71	6.75961558180756E-4
72-73	0.006759052754116907
74-75	0.0060245532190160685
76-77	0.00469366098563799
78-79	0.002434376757578047
80-81	3.641616392129253E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020995742826836273
90-91	2.964625062579368E-4
92-93	0.0
94-95	7.937102169289394E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	757.0
20-21	1998.0
22-23	1304.0
24-25	1450.0
26-27	2354.0
28-29	4784.0
30-31	13751.0
32-33	17161.0
34-35	23139.0
36-37	35917.0
38-39	43577.0
40-41	41322.0
42-43	46122.0
44-45	54064.0
46-47	54625.0
48-49	51564.0
50-51	62015.0
52-53	42998.0
54-55	45541.0
56-57	46745.0
58-59	48813.0
60-61	47662.0
62-63	46540.0
64-65	48378.0
66-67	51279.0
68-69	52445.0
70-71	52623.0
72-73	47297.0
74-75	47096.0
76-77	48498.0
78-79	50097.0
80-81	50262.0
82-83	47114.0
84-85	45449.0
86-87	47402.0
88-89	49476.0
90-91	50500.0
92-93	49496.0
94-95	52769.0
96-97	149305.0
98-99	1136460.0
100-101	2453532.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.56197414502782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.08675510964953	75.33351882246609
2	7.568737821127053	12.8005482388128
3	1.9334848718968252	4.904978932414252
4	0.70022168630114	2.368485125311391
5	0.3017352700263327	1.2757665051304865
6	0.15525234190784415	0.7877066713419681
7	0.08590835529501162	0.5085206083509045
8	0.04561503399263513	0.3085837860087821
9	0.03338916697685772	0.25411084871589534
>10	0.085925317195181	1.1060109940962004
>50	0.0016454333163444714	0.09323825604453757
>100	0.0012940129423791278	0.22624826800272396
>500	0.0	0.0
>1k	3.557937281519975E-5	0.03228294330394179
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.8998111777670416E-5	0.0
5	0.0	0.0	0.0	1.8998111777670416E-5	0.0
6	0.0	0.0	0.0	1.8998111777670416E-5	0.0
7	0.0	0.0	0.0	1.8998111777670416E-5	0.0
8	0.0	0.0	0.0	1.8998111777670416E-5	0.0
9	0.0	3.799622355534083E-5	0.0	1.8998111777670416E-5	0.0
10-11	2.8497167666505627E-5	3.799622355534083E-5	0.0	1.8998111777670416E-5	0.0
12-13	5.6994335333011254E-5	3.799622355534083E-5	0.0	2.8497167666505624E-5	0.0
14-15	5.6994335333011254E-5	1.1398867066602251E-4	0.0	5.6994335333011254E-5	0.0
16-17	5.6994335333011254E-5	1.1398867066602251E-4	0.0	5.6994335333011254E-5	0.0
18-19	5.6994335333011254E-5	1.1398867066602251E-4	0.0	5.6994335333011254E-5	0.0
20-21	5.6994335333011254E-5	1.6148395011019855E-4	0.0	6.649339122184647E-5	0.0
22-23	9.499055888835209E-5	1.8998111777670417E-4	0.0	9.499055888835209E-5	0.0
24-25	1.5198489422136333E-4	2.089792295543746E-4	0.0	1.3298678244369293E-4	0.0
26-27	1.7098300599903375E-4	2.089792295543746E-4	0.0	2.7547262077622103E-4	0.0
28-29	1.8998111777670417E-4	2.089792295543746E-4	0.0	6.554348563296295E-4	0.0
30-31	1.8998111777670417E-4	2.8497167666505626E-4	0.0	0.0019093102336558768	0.0
32-33	1.8998111777670417E-4	3.0396978844272665E-4	0.0	0.004958507173971978	0.0
34-35	1.8998111777670417E-4	3.609641237757379E-4	0.0	0.00906209931794879	0.0
36-37	2.469754531097154E-4	4.179584591087492E-4	0.0	0.015407468651690709	0.0
38-39	2.469754531097154E-4	4.179584591087492E-4	0.0	0.03027349111771781	0.0
40-41	2.469754531097154E-4	4.5595468266409003E-4	0.0	0.045319995645632785	0.0
42-43	2.469754531097154E-4	4.749527944417604E-4	0.0	0.05516101754646606	0.0
44-45	2.469754531097154E-4	5.319471297747717E-4	0.0	0.06713932702228725	0.0
46-47	2.469754531097154E-4	5.699433533301125E-4	0.0	0.08065648355209976	0.0
48-49	2.6597356488738586E-4	5.88941465107783E-4	0.0	0.09325223166069524	0.0
50-51	3.0396978844272665E-4	6.934310798849702E-4	0.0	0.10590497410462374	0.0
52-53	3.0396978844272665E-4	7.029301357738054E-4	0.0	0.1347916030625716	0.0
54-55	3.134688443315619E-4	7.029301357738054E-4	0.0	0.16909269387715553	0.0
56-57	3.229679002203971E-4	7.219282475514759E-4	0.0	0.22030210417386614	0.0
58-59	3.4196601199806755E-4	7.219282475514759E-4	0.0	0.25950470782708907	0.0
60-61	3.7046317966457317E-4	7.314273034403111E-4	0.0	0.27669799898588077	0.0
62-63	3.7996223555340834E-4	7.599244711068167E-4	0.0	0.2906141158630244	0.0
64-65	3.8946129144224357E-4	8.739131417728392E-4	0.0	0.2973014512087644	0.0
66-67	3.989603473310788E-4	8.929112535505096E-4	0.0	0.30374181110139464	0.0
68-69	3.989603473310788E-4	9.119093653281801E-4	0.0	0.30994469459680407	0.0
70-71	3.989603473310788E-4	9.119093653281801E-4	0.0	0.3160145913097697	0.0
72-73	3.989603473310788E-4	9.119093653281801E-4	0.0	0.3208591098130757	0.0
74-75	3.989603473310788E-4	9.214084212170152E-4	0.0	0.3245162463302772	0.0
76-77	3.989603473310788E-4	9.689037006611913E-4	0.0	0.32838236207703314	0.0
78-79	3.989603473310788E-4	9.879018124388616E-4	0.0	0.33080462132868615	0.0
80-81	3.989603473310788E-4	9.879018124388616E-4	0.0	0.3314410580732381	0.0
82-83	3.989603473310788E-4	9.97400868327697E-4	0.0	0.3318400184205692	0.0
84-85	3.989603473310788E-4	0.0010353970918830378	0.0	0.3320299995383459	0.0
86-87	3.989603473310788E-4	0.0011113895389937194	0.0	0.3320964929295677	0.0
88	3.989603473310788E-4	0.001139886706660225	0.0	0.33210599198545654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8140	0.0	52.134686	1
GTATCAA	15450	0.0	37.4241	1
ATCAACG	19000	0.0	30.36588	3
TCAACGC	19195	0.0	30.079115	4
CAACGCA	19485	0.0	29.588652	5
TATCAAC	19960	0.0	29.30222	2
AACGCAG	20170	0.0	28.685486	6
ACGCAGA	22985	0.0	25.154205	7
CGCAGAG	23270	0.0	24.846127	8
CCGAACC	845	0.0	23.185678	9
GGACCGA	1020	0.0	22.477146	6
GTACATG	22375	0.0	22.115208	1
ACCGAAC	890	0.0	22.013367	8
TGGTATC	3810	0.0	21.992493	2
TACATGG	22490	0.0	21.798231	2
GTGGTAT	3930	0.0	21.745264	1
GCAGAGT	26850	0.0	21.548836	9
ACATGGG	22815	0.0	21.15882	3
GAGTACT	13620	0.0	20.29154	12-13
AGGACCG	1275	0.0	19.290577	5
>>END_MODULE