FastQCFastQC Report
Fri 27 May 2016
SRR2138865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5109794
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70850.054.1790961
GTATCAA142350.040.8962171
ATCAACG173100.033.2388043
TCAACGC174100.033.0478864
TATCAAC179150.032.424442
CAACGCA177750.032.4170465
AACGCAG183050.031.476296
ACGCAGA207900.027.713977
CGCAGAG211750.027.2100838
GCAGAGT246150.023.3211659
GTACATG180250.022.3088861
TACATGG182150.022.0062472
ACATGGG179950.021.7089653
GTGGTAT31900.021.5639081
GAGTACT144350.020.76640712-13
CATGGGG128350.019.9822434
TGGTATC32350.019.8200992
CAGAGTA240250.019.69654710-11
AGAGTAC222150.019.48562410-11
GTACTTT161400.018.5992614-15
Produced by FastQC (version 0.11.5)