##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2138865_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5109794 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.297791652657622 32.0 25.0 33.0 18.0 33.0 2 31.05193594888561 33.0 32.0 33.0 27.0 33.0 3 32.062643621249705 33.0 31.0 33.0 29.0 34.0 4 32.26303858824837 33.0 33.0 33.0 31.0 34.0 5 33.03204767158911 33.0 33.0 34.0 33.0 34.0 6 37.024229939602264 38.0 37.0 38.0 36.0 38.0 7 37.16047652801659 38.0 38.0 38.0 36.0 38.0 8 37.229526669763985 38.0 38.0 38.0 36.0 38.0 9 37.40533747544421 38.0 38.0 38.0 37.0 38.0 10-11 37.48348954967656 38.0 38.0 38.0 37.0 38.0 12-13 37.519731617360705 38.0 38.0 38.0 37.0 38.0 14-15 37.521795888444814 38.0 38.0 38.0 37.0 38.0 16-17 37.522893192954555 38.0 38.0 38.0 37.0 38.0 18-19 37.52349527202075 38.0 38.0 38.0 37.0 38.0 20-21 37.52149433245733 38.0 38.0 38.0 37.0 38.0 22-23 37.53169042819653 38.0 38.0 38.0 37.5 38.0 24-25 37.49266901057825 38.0 38.0 38.0 37.0 38.0 26-27 37.499870727844566 38.0 38.0 38.0 37.0 38.0 28-29 37.49187426291536 38.0 38.0 38.0 37.0 38.0 30-31 37.48332383547307 38.0 38.0 38.0 37.0 38.0 32-33 37.47977677904427 38.0 38.0 38.0 37.0 38.0 34-35 37.46465658042746 38.0 38.0 38.0 37.0 38.0 36-37 37.44071899031462 38.0 38.0 38.0 37.0 38.0 38-39 37.4143297408565 38.0 38.0 38.0 37.0 38.0 40-41 37.392282643089075 38.0 38.0 38.0 37.0 38.0 42-43 37.36940661528739 38.0 38.0 38.0 37.0 38.0 44-45 37.33242756082143 38.0 38.0 38.0 37.0 38.0 46-47 37.28832106906934 38.0 38.0 38.0 37.0 38.0 48-49 37.240278743692755 38.0 38.0 38.0 37.0 38.0 50-51 37.21768299482409 38.0 38.0 38.0 37.0 38.0 52-53 37.20575466286392 38.0 38.0 38.0 37.0 38.0 54-55 37.18478871701483 38.0 38.0 38.0 37.0 38.0 56-57 37.150575911388664 38.0 38.0 38.0 37.0 38.0 58-59 37.1550142761058 38.0 38.0 38.0 37.0 38.0 60-61 37.17316797284242 38.0 38.0 38.0 37.0 38.0 62-63 37.18054545952249 38.0 38.0 38.0 37.0 38.0 64-65 37.18906758099009 38.0 38.0 38.0 37.0 38.0 66-67 37.198676785869885 38.0 38.0 38.0 37.0 38.0 68-69 37.199249451299444 38.0 38.0 38.0 37.0 38.0 70-71 37.16774974829786 38.0 38.0 38.0 36.5 38.0 72-73 37.14854216594522 38.0 38.0 38.0 36.0 38.0 74-75 37.13248308777902 38.0 38.0 38.0 36.0 38.0 76-77 37.13448580402316 38.0 38.0 38.0 36.0 38.0 78-79 37.13718259442874 38.0 38.0 38.0 36.0 38.0 80-81 37.12797755587634 38.0 38.0 38.0 36.0 38.0 82-83 37.13186518286935 38.0 38.0 38.0 36.0 38.0 84-85 37.120357836376634 38.0 38.0 38.0 36.0 38.0 86-87 37.092451545360504 38.0 38.0 38.0 36.0 38.0 88-89 37.05320257428096 38.0 38.0 38.0 36.0 38.0 90-91 37.05943860816336 38.0 38.0 38.0 36.0 38.0 92-93 37.038590015889184 38.0 38.0 38.0 36.0 38.0 94-95 37.02395621084462 38.0 38.0 38.0 36.0 38.0 96-97 37.014303368577146 38.0 38.0 38.0 36.0 38.0 98-99 37.05366387204727 38.0 38.0 38.0 36.0 38.0 100 35.38258252652412 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 9.0 19 16.0 20 52.0 21 125.0 22 353.0 23 853.0 24 1883.0 25 3663.0 26 6407.0 27 10705.0 28 16349.0 29 24770.0 30 35067.0 31 46539.0 32 60736.0 33 79172.0 34 115028.0 35 204911.0 36 623523.0 37 3879633.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.01687856692461 19.766139300331872 14.191413587318785 25.025568545424726 2 13.914102995150099 20.68433678539683 43.33356687177605 22.067993347677028 3 17.804279389736653 26.882003462370495 30.399053269075033 24.914663878817816 4 12.129216950820327 18.247858915643175 39.26847148828309 30.35445264525341 5 13.08815593982134 37.80300173038994 35.61479535266702 13.494046977121704 6 29.22625813340347 38.24770166769998 19.12422266894621 13.401817529950339 7 26.02224277534476 33.113291846990315 23.4651338194847 17.39933155818023 8 24.004470630322867 36.96344705872683 21.402056521260935 17.63002578968937 9 25.59819937911379 17.040338033262795 21.715654626322436 35.645807961300974 10-11 23.97775213203353 27.04427557045853 29.011644894421355 19.966327403086584 12-13 24.964176638040595 24.687903269681712 29.259506351919473 21.088413740358224 14-15 22.052229502794045 25.821109422415073 27.24327047235172 24.88339060243916 16-17 21.24781351263867 29.000777722154748 27.875634516773083 21.8757742484335 18-19 21.319019905694827 27.90031261534222 30.00017221829295 20.780495260669998 20-21 22.210763685539003 27.072970133925928 29.823547341810304 20.892718838724768 22-23 21.958069207228796 26.933665339834505 29.747500679366638 21.36076477357006 24-25 21.640925841782664 27.098361074459042 29.836307084539033 21.424405999219257 26-27 21.570989421146685 27.301523793733846 29.696541968017687 21.430944817101786 28-29 21.4389578856161 27.386891328930812 29.75101455376768 21.423136231685408 30-31 21.784575252413436 27.153174773289283 29.696112033860743 21.366137940436538 32-33 21.550434363408414 27.28902699531946 29.641437534451846 21.519101106820276 34-35 21.67856621836775 27.31970334669289 29.65562798180379 21.346102453135572 36-37 21.693226740867495 27.23589251405362 29.635993732973965 21.43488701210492 38-39 21.48068037259637 27.407164457220894 29.671220931968712 21.44093423821402 40-41 21.788926158183852 27.170478709142635 29.609574483546297 21.431020649127213 42-43 21.695264526290643 27.26324574276873 29.40505491428918 21.63643481665144 44-45 21.714783978283364 27.4062640854421 29.301494341404155 21.57745759487038 46-47 21.76381653167386 27.425132687659143 29.039181211524017 21.77186956914298 48-49 21.761912228240917 27.535329250906642 28.944123184591476 21.758635336260966 50-51 21.701231712346708 27.669181886690254 28.780597765133365 21.848988635829677 52-53 21.857158579303366 27.582926746655534 28.708802267874173 21.851112406166926 54-55 21.834122774080246 27.572791471699798 28.710883858072982 21.88220189614697 56-57 21.878262690360366 27.64180504433853 28.616901944197394 21.863030321103707 58-59 21.79560223568172 27.615810391330886 28.773210783570942 21.815376589416445 60-61 21.89010096870135 27.64251283614769 28.72806483337395 21.739321361777 62-63 21.834510611821763 27.656549229848647 28.696907268318515 21.81203289001107 64-65 21.788061860258125 27.653905206942593 28.672863818424567 21.885169114374722 66-67 21.85728161284687 27.68402124141976 28.535868911250837 21.922828234482534 68-69 21.834672640266213 27.642289252152686 28.594150038099407 21.928888069481694 70-71 21.846032051079796 27.44904497878846 28.63017918658983 22.074743783541912 72-73 21.928093014767562 27.458587064413575 28.6197991577989 21.99352076301995 74-75 21.842032422123626 27.64941945449189 28.46574498149308 22.0428031418914 76-77 21.98916310175227 27.548218000093666 28.3700326743741 22.09258622377996 78-79 21.968707145610068 27.58556238404106 28.34709553671996 22.098634933628915 80-81 21.915589416356212 27.6378564094969 28.307950417150735 22.138603756996154 82-83 21.975840451192514 27.611118770303563 28.348497516773126 22.064543261730808 84-85 22.12402406585525 27.63369737938417 28.181561659963133 22.06071689479745 86-87 21.96196008503072 27.751427141984625 28.251607852837164 22.035004920147497 88-89 22.114141055753404 27.704871025862886 28.141563696794346 22.03942422158936 90-91 22.108020504551902 27.6898775124431 28.173680606840097 22.028421376164907 92-93 22.170966297327432 27.68131992968332 28.146930040884822 22.000783732104427 94-95 22.013176568443576 27.800320836905033 28.167546943954775 22.018955650696615 96-97 22.152838488728698 27.840195447468524 28.18914818494601 21.817817878856772 98-99 23.123465487122438 29.838581878550823 28.406792161445892 18.631160472880843 100 22.021964784333342 30.672252408345145 22.395767257138647 24.91001555018287 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1354.0 1 1234.5 2 1726.5 3 2581.5 4 3220.5 5 4195.5 6 5168.0 7 6783.0 8 9068.0 9 10323.0 10 10155.0 11 9940.0 12 10747.5 13 12672.0 14 16043.0 15 19686.0 16 22269.0 17 23488.5 18 22905.5 19 21069.5 20 19120.0 21 18046.5 22 18966.5 23 22093.0 24 27313.0 25 34768.5 26 44816.0 27 55789.5 28 66987.5 29 79677.5 30 92156.0 31 104367.5 32 117856.0 33 130325.5 34 141092.5 35 151330.5 36 163479.5 37 175437.5 38 182914.5 39 187925.0 40 192933.5 41 197232.0 42 200760.0 43 204784.5 44 207433.0 45 210186.5 46 211504.0 47 210299.0 48 208190.0 49 204046.5 50 198426.0 51 192203.5 52 184797.0 53 175509.5 54 165454.5 55 153998.0 56 141252.5 57 126840.5 58 112211.5 59 98047.0 60 82865.5 61 67667.0 62 52883.0 63 40722.0 64 31540.5 65 23508.0 66 16987.0 67 12675.5 68 9523.5 69 6921.5 70 5016.5 71 3569.5 72 2526.5 73 1738.5 74 1171.5 75 784.5 76 477.5 77 271.5 78 167.0 79 113.5 80 89.0 81 58.0 82 34.0 83 23.5 84 15.0 85 12.0 86 12.0 87 8.0 88 4.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.01058751096423848 6 3.914052112472636E-5 7 0.0 8 0.0 9 1.957026056236318E-5 10-11 1.957026056236318E-5 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.165272760474456E-4 26-27 0.0 28-29 7.24501180936925E-4 30-31 0.0025775560143104343 32-33 1.8662406399437377E-4 34-35 7.88038288022281E-5 36-37 3.066631067366663E-4 38-39 5.574182615796676E-4 40-41 6.915903812407011E-4 42-43 1.3120350349687615E-4 44-45 0.0 46-47 0.001561278806492742 48-49 7.258932661059383E-5 50-51 0.00214530203969039 52-53 0.001709360044088827 54-55 0.0028399913757864888 56-57 0.00267095678820986 58-59 0.003917917676540613 60-61 0.0030257688456790163 62-63 0.0032629664333351707 64-65 0.00467267884678286 66-67 0.0017526729104515595 68-69 1.1352611429854598E-4 70-71 8.260815041842749E-4 72-73 0.00704895744412223 74-75 0.006170154599253142 76-77 0.005002377607607587 78-79 0.002784430801397426 80-81 6.0401442483088805E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0022056608601069053 90-91 2.0396916343194945E-4 92-93 0.0 94-95 1.1740785375051562E-4 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 38.0 20-21 158.0 22-23 262.0 24-25 418.0 26-27 1296.0 28-29 3398.0 30-31 10708.0 32-33 13532.0 34-35 18758.0 36-37 29374.0 38-39 34988.0 40-41 33757.0 42-43 38053.0 44-45 45302.0 46-47 46698.0 48-49 44818.0 50-51 40422.0 52-53 37376.0 54-55 39581.0 56-57 40407.0 58-59 42255.0 60-61 42175.0 62-63 41289.0 64-65 42975.0 66-67 45743.0 68-69 46437.0 70-71 46115.0 72-73 42613.0 74-75 42135.0 76-77 43518.0 78-79 44859.0 80-81 44925.0 82-83 42561.0 84-85 41706.0 86-87 42969.0 88-89 44781.0 90-91 44736.0 92-93 44159.0 94-95 50602.0 96-97 152370.0 98-99 330955.0 100-101 3310572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.01969527838821 #Duplication Level Percentage of deduplicated Percentage of total 1 89.96464035510179 77.38730949175643 2 7.193244611111946 12.375214190215154 3 1.687453661027234 4.354627491538897 4 0.5631814484621523 1.9377878633262253 5 0.24085368907831037 1.0359080470595958 6 0.1311835677239418 0.6770622312687167 7 0.06828412896995359 0.4111645976441898 8 0.039567614571403176 0.2722875318659851 9 0.0284912523331655 0.2205727959418707 >10 0.08083231224466032 1.0423983301857145 >50 0.0012942565119743292 0.07977452366933549 >100 8.354602493524915E-4 0.12274667597924102 >500 1.3764261422638484E-4 0.08314622954856202 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.957026056236318E-5 0.0 1.957026056236318E-5 0.0 3 0.0 1.957026056236318E-5 0.0 1.957026056236318E-5 0.0 4 0.0 3.914052112472636E-5 0.0 1.957026056236318E-5 0.0 5 0.0 3.914052112472636E-5 0.0 1.957026056236318E-5 0.0 6 0.0 3.914052112472636E-5 0.0 1.957026056236318E-5 0.0 7 0.0 5.871078168708954E-5 0.0 1.957026056236318E-5 0.0 8 0.0 5.871078168708954E-5 0.0 1.957026056236318E-5 0.0 9 0.0 1.1742156337417908E-4 0.0 5.871078168708954E-5 0.0 10-11 0.0 1.1742156337417908E-4 0.0 5.871078168708954E-5 0.0 12-13 0.0 1.1742156337417908E-4 0.0 5.871078168708954E-5 0.0 14-15 0.0 1.4677695421772384E-4 0.0 8.806617253063432E-5 0.0 16-17 0.0 1.5656208449890544E-4 0.0 9.78513028118159E-5 0.0 18-19 0.0 1.859174753424502E-4 0.0 9.78513028118159E-5 0.0 20-21 0.0 2.1527286618599497E-4 0.0 9.78513028118159E-5 0.0 22-23 0.0 2.1527286618599497E-4 0.0 1.1742156337417908E-4 0.0 24-25 0.0 2.5441338731072136E-4 0.0 1.5656208449890544E-4 0.0 26-27 0.0 2.935539084354477E-4 0.0 2.250579964671766E-4 0.0 28-29 0.0 3.033390387166293E-4 0.0 4.99041644340261E-4 0.0 30-31 0.0 3.914052112472636E-4 0.0 0.0013992736302089674 0.0 32-33 0.0 3.914052112472636E-4 0.0 0.0037281346371301856 0.0 34-35 0.0 4.5990112321553475E-4 0.0 0.006595177809516391 0.0 36-37 0.0 5.283970351838059E-4 0.0 0.011233329562796466 0.0 38-39 0.0 5.47967295746169E-4 0.0 0.02251558477699884 0.0 40-41 0.0 6.066780774332585E-4 0.0 0.03521668388197254 0.0 42-43 0.0 6.262483379956218E-4 0.0 0.043074143497761355 0.0 44-45 0.0 6.849591196827113E-4 0.0 0.0527222819550064 0.0 46-47 0.0 7.240996408074377E-4 0.0 0.06298688361996589 0.0 48-49 0.0 7.240996408074377E-4 0.0 0.07279158416170985 0.0 50-51 0.0 8.904468555875247E-4 0.0 0.08265499548514088 0.0 52-53 0.0 9.002319858687063E-4 0.0 0.10906506211405 0.0 54-55 0.0 9.100171161498878E-4 0.0 0.14072974370395364 0.0 56-57 0.0 9.393725069934326E-4 0.0 0.19040885014151254 0.0 58-59 0.0 9.491576372746142E-4 0.0 0.22685846043891397 0.0 60-61 0.0 9.687278978369774E-4 0.0 0.24051850231144348 0.0 62-63 0.0 9.98083288680522E-4 0.0 0.25120386457849375 0.0 64-65 0.0 0.0010763643309299748 0.0 0.25598879328599156 0.0 66-67 0.0 0.0010763643309299748 0.0 0.261292333898392 0.0 68-69 0.0 0.001095934591492338 0.0 0.2666056596410736 0.0 70-71 0.0 0.001095934591492338 0.0 0.27141015860913376 0.0 72-73 0.0 0.001095934591492338 0.0 0.27502087168288974 0.0 74-75 0.0 0.0011546453731794276 0.0 0.2782499646756797 0.0 76-77 0.0 0.0012427115457100619 0.0 0.2814399171473449 0.0 78-79 0.0 0.0012720669365536067 0.0 0.2836513565908919 0.0 80-81 0.0 0.0012720669365536067 0.0 0.2842091090169193 0.0 82-83 0.0 0.0012720669365536067 0.0 0.28453201831619823 0.0 84-85 0.0 0.0012720669365536067 0.0 0.2847277209218219 0.0 86-87 0.0 0.001311207457678333 0.0 0.28480600196407135 0.0 88 0.0 0.0013699182393654226 0.0 0.28480600196407135 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7085 0.0 54.179096 1 GTATCAA 14235 0.0 40.896217 1 ATCAACG 17310 0.0 33.238804 3 TCAACGC 17410 0.0 33.047886 4 TATCAAC 17915 0.0 32.42444 2 CAACGCA 17775 0.0 32.417046 5 AACGCAG 18305 0.0 31.47629 6 ACGCAGA 20790 0.0 27.71397 7 CGCAGAG 21175 0.0 27.210083 8 GCAGAGT 24615 0.0 23.321165 9 GTACATG 18025 0.0 22.308886 1 TACATGG 18215 0.0 22.006247 2 ACATGGG 17995 0.0 21.708965 3 GTGGTAT 3190 0.0 21.563908 1 GAGTACT 14435 0.0 20.766407 12-13 CATGGGG 12835 0.0 19.982243 4 TGGTATC 3235 0.0 19.820099 2 CAGAGTA 24025 0.0 19.696547 10-11 AGAGTAC 22215 0.0 19.485624 10-11 GTACTTT 16140 0.0 18.59926 14-15 >>END_MODULE