FastQCFastQC Report
Fri 27 May 2016
SRR2138864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4986368
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.047.652031
GTATCAA111950.037.435011
TCAACGC135350.030.9948774
ATCAACG135750.030.840093
CAACGCA139600.030.1129725
TATCAAC142200.029.683542
AACGCAG144400.029.1985426
CGAGACC35100.027.24534686-87
ACGAGAC36450.026.01265386-87
GAGACCG37150.025.71040388-89
ACGCAGA164850.025.6547747
AGACCGA38050.025.5350788-89
GACCGAG38750.025.31078390-91
CGCAGAG167050.025.1880058
ACCGAGG40350.024.65240590-91
TCTCCGA36400.024.64916274-75
CCCACGA38400.024.64095782-83
ATCTCCG35900.024.41957574-75
CACGAGA39300.024.23473584-85
TGGTATC29700.024.218612
Produced by FastQC (version 0.11.5)