##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4986368
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.56338862274104	32.0	25.0	33.0	18.0	33.0
2	31.142901005300853	33.0	32.0	33.0	27.0	33.0
3	32.05636286772256	33.0	31.0	33.0	29.0	34.0
4	32.23707175242581	33.0	33.0	33.0	31.0	34.0
5	33.020908805768045	33.0	33.0	34.0	33.0	34.0
6	36.95611194360304	38.0	37.0	38.0	35.0	38.0
7	37.08593489289198	38.0	38.0	38.0	36.0	38.0
8	37.14995363358661	38.0	38.0	38.0	36.0	38.0
9	37.34585694437314	38.0	38.0	38.0	37.0	38.0
10-11	37.427290665269794	38.0	38.0	38.0	37.0	38.0
12-13	37.47024106524027	38.0	38.0	38.0	37.0	38.0
14-15	37.47175729910027	38.0	38.0	38.0	37.0	38.0
16-17	37.470503781509905	38.0	38.0	38.0	37.0	38.0
18-19	37.47006368162157	38.0	38.0	38.0	37.0	38.0
20-21	37.46953368006299	38.0	38.0	38.0	37.0	38.0
22-23	37.483355064198136	38.0	38.0	38.0	37.0	38.0
24-25	37.44484475062094	38.0	38.0	38.0	37.0	38.0
26-27	37.454001937093565	38.0	38.0	38.0	37.0	38.0
28-29	37.44693951603003	38.0	38.0	38.0	37.0	38.0
30-31	37.44093607177847	38.0	38.0	38.0	37.0	38.0
32-33	37.43826161719801	38.0	38.0	38.0	37.0	38.0
34-35	37.42589636744853	38.0	38.0	38.0	37.0	38.0
36-37	37.402370323420016	38.0	38.0	38.0	37.0	38.0
38-39	37.38073763642305	38.0	38.0	38.0	37.0	38.0
40-41	37.3625675177226	38.0	38.0	38.0	37.0	38.0
42-43	37.34337227529848	38.0	38.0	38.0	37.0	38.0
44-45	37.31195732195148	38.0	38.0	38.0	37.0	38.0
46-47	37.27510492131559	38.0	38.0	38.0	37.0	38.0
48-49	37.23026885076749	38.0	38.0	38.0	37.0	38.0
50-51	37.20940275229022	38.0	38.0	38.0	37.0	38.0
52-53	37.19555798400928	38.0	38.0	38.0	37.0	38.0
54-55	37.17140932835662	38.0	38.0	38.0	37.0	38.0
56-57	37.13513125157161	38.0	38.0	38.0	36.5	38.0
58-59	37.13688817135491	38.0	38.0	38.0	36.0	38.0
60-61	37.14780302777133	38.0	38.0	38.0	36.0	38.0
62-63	37.15423266285845	38.0	38.0	38.0	36.0	38.0
64-65	37.15880052093472	38.0	38.0	38.0	36.0	38.0
66-67	37.16052999352678	38.0	38.0	38.0	36.0	38.0
68-69	37.16029950021506	38.0	38.0	38.0	36.0	38.0
70-71	37.12471063294889	38.0	38.0	38.0	36.0	38.0
72-73	37.09528425832569	38.0	38.0	38.0	36.0	38.0
74-75	37.07458671639584	38.0	38.0	38.0	36.0	38.0
76-77	37.07229549837423	38.0	38.0	38.0	36.0	38.0
78-79	37.0721367676735	38.0	38.0	38.0	36.0	38.0
80-81	37.057986850985166	38.0	38.0	38.0	36.0	38.0
82-83	37.0550366355217	38.0	38.0	38.0	36.0	38.0
84-85	37.043488351758924	38.0	38.0	38.0	36.0	38.0
86-87	37.006890089249	38.0	38.0	38.0	36.0	38.0
88-89	36.961437110328916	38.0	38.0	38.0	36.0	38.0
90-91	36.964643088862914	38.0	38.0	38.0	36.0	38.0
92-93	36.93655835589328	38.0	38.0	38.0	35.5	38.0
94-95	36.91414894776038	38.0	38.0	38.0	35.0	38.0
96-97	36.89728569755028	38.0	38.0	38.0	35.0	38.0
98-99	36.937639027774004	38.0	38.0	38.0	35.0	38.0
100	35.211523527758324	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	15.0
20	35.0
21	142.0
22	350.0
23	903.0
24	2103.0
25	3848.0
26	6632.0
27	10811.0
28	16837.0
29	25130.0
30	35217.0
31	47222.0
32	62811.0
33	83756.0
34	123907.0
35	218130.0
36	628800.0
37	3719714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99621207259472	19.39491830526748	14.185495334479924	25.423374287657868
2	14.0293496188007	20.288013239295616	43.08659128247253	22.59604585943115
3	18.177960391210597	25.88318792355478	30.034887116233698	25.903964569000927
4	12.363969125423555	17.933734533833043	38.54143938032652	31.160856960416883
5	13.562485559149723	37.314074052680226	35.37344439951735	13.7499959886527
6	30.224462108168975	37.53674269733563	18.739302076763334	13.499493117732056
7	26.775661162593696	32.446000776517096	22.875688276517096	17.902649784372112
8	24.182491143854605	37.10526379120033	21.0036042265633	17.708640838381765
9	25.482916623883355	17.159042413235444	21.327166386435977	36.03087457644522
10-11	24.337999990774847	26.746928174360214	28.672648844338976	20.24242299052597
12-13	25.114030893828936	24.2348338510114	28.914181624781804	21.73695363037786
14-15	22.164218525387618	25.554411547643497	26.719798859610844	25.561571067358045
16-17	21.76285424581579	28.73714896293254	27.335677992478697	22.164318798772975
18-19	21.78939661092001	27.815375840692063	29.204904250949788	21.190323297438134
20-21	22.556350943085565	26.96396148781912	29.15097581512292	21.328711753972396
22-23	22.30458779847424	26.82055761619912	29.022998908080076	21.85185567724656
24-25	22.042803123103077	26.93991796223511	29.112365343331447	21.904913571330365
26-27	21.99747165642609	27.12091269091075	29.000542074455254	21.881073578207904
28-29	21.882202250788957	27.207285781097923	29.04256499493692	21.867946973176203
30-31	22.189087165959677	27.01731479332052	28.962839381917465	21.830758658802342
32-33	22.005934973603843	27.15670384348939	28.933182409052883	21.90417877385389
34-35	22.115833809688073	27.166397323311546	28.952916657297546	21.764852209702834
36-37	22.10215785957428	27.060484792330886	28.92812754441899	21.909229803675846
38-39	21.899985889662858	27.191311450543676	29.0459001026789	21.862802557114566
40-41	22.1563924425794	27.050254798492624	28.91814267794684	21.875210080981134
42-43	22.08533830136535	27.159791877885226	28.7310829657916	22.023786854957823
44-45	22.128163709141997	27.234757100474035	28.654084965863873	21.982994224520098
46-47	22.174593310853137	27.223266041795778	28.442926030571	22.159214616780076
48-49	22.096971110065713	27.30326106084392	28.44643371855422	22.153334110536143
50-51	22.08720560166279	27.475554487742066	28.324628931864137	22.11261097873101
52-53	22.256288425318317	27.35464345653405	28.240495331663407	22.148572786484223
54-55	22.189263686004374	27.259352302905548	28.297777488032406	22.25360652305767
56-57	22.232245618978467	27.3850313742915	28.216531379710208	22.16619162701982
58-59	22.160226794388052	27.378654198193903	28.21191839265812	22.24920061475993
60-61	22.341229625829868	27.296654323704477	28.40176155433123	21.960354496134425
62-63	22.09227641373009	27.343560567487774	28.344325360009194	22.21983765877295
64-65	22.094663376976193	27.33880564977368	28.300079418875836	22.26645155437429
66-67	22.141570590003905	27.4048538969706	28.319468133832004	22.13410737919349
68-69	22.106074598832322	27.542690678338538	28.233104310684777	22.118130412144367
70-71	22.175316243370187	27.307661800350996	28.047607018674782	22.469414937604036
72-73	22.196098605436994	27.313099901697633	27.98415704538968	22.506644447475686
74-75	22.0694410114133	27.48993639761358	28.16169743659156	22.278925154381565
76-77	22.287168300214518	27.184474917284714	28.00225736154009	22.52609942096068
78-79	22.20653813143189	27.249609696183192	27.811189476228048	22.73266269615687
80-81	22.407775390068828	27.449538618103436	27.798721601501686	22.343964390326057
82-83	22.48243562548456	27.260733234028113	27.80258260430099	22.454248536186338
84-85	22.33779564082356	27.28489221525133	27.72616145860952	22.65115068531559
86-87	22.179809269641083	27.493003338912946	27.825024921299864	22.502162470146104
88-89	22.492353805810264	27.528546867929105	27.713995329574935	22.2651039966857
90-91	22.55222975511041	27.539935421039395	27.654657454956833	22.253177368893358
92-93	22.364980917601997	27.285827541133635	27.70164215290698	22.647549388357394
94-95	22.45828613121868	27.600378896974586	27.660336783087878	22.280998188718854
96-97	22.773045208541625	27.463479312143633	27.746485003569727	22.01699047574501
98-99	23.48493250293994	29.522133920311262	28.06102109076151	18.931912485987286
100	22.32344057436601	30.35844874488449	22.11087012448726	25.20724055626224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1198.0
1	1059.0
2	1365.0
3	1937.5
4	2310.5
5	2951.0
6	3661.5
7	4928.5
8	6696.0
9	7756.5
10	7744.0
11	7671.0
12	8334.0
13	9731.0
14	12346.0
15	15118.5
16	17077.0
17	18109.0
18	17614.5
19	16359.0
20	15235.5
21	14653.0
22	15566.5
23	18157.5
24	22732.5
25	29854.0
26	38902.0
27	48502.0
28	58185.0
29	69450.5
30	80764.5
31	91323.5
32	102824.0
33	113844.0
34	124475.5
35	135510.0
36	147350.0
37	157530.5
38	165093.5
39	171500.0
40	178500.0
41	185466.0
42	190034.0
43	194685.5
44	198431.5
45	202339.0
46	205670.5
47	206759.5
48	206397.0
49	203672.5
50	200668.0
51	195880.0
52	187315.5
53	177805.0
54	169128.0
55	158573.0
56	145897.5
57	132411.5
58	118694.5
59	103902.0
60	87145.5
61	71127.0
62	56554.5
63	43713.0
64	33214.0
65	24743.0
66	18516.0
67	14342.0
68	10892.5
69	7892.0
70	5731.5
71	4142.0
72	2918.0
73	1961.5
74	1289.5
75	854.5
76	541.0
77	339.0
78	223.0
79	137.5
80	84.0
81	51.0
82	35.0
83	22.5
84	17.0
85	17.5
86	13.0
87	8.0
88	4.5
89	3.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.010267994660642776
6	6.016403121470377E-5
7	0.0
8	0.0
9	0.0
10-11	2.005467707156792E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.61583384352181E-4
26-27	0.0
28-29	6.921937697143557E-4
30-31	0.0024096830704502976
32-33	1.5088381704673296E-4
34-35	5.0416775314817474E-5
36-37	2.6310601917759537E-4
38-39	5.1883450644189E-4
40-41	5.934672556999716E-4
42-43	1.7497872207275264E-4
44-45	0.0
46-47	0.0013894958501609463
48-49	1.0531192285775276E-4
50-51	0.002122375761959428
52-53	0.0016027266012333943
54-55	0.002969084727996339
56-57	0.0027201150120980096
58-59	0.0038592717641396253
60-61	0.002972841719654005
62-63	0.002974166105739676
64-65	0.0045357867444697116
66-67	0.0017084166607939843
68-69	1.7011937162894289E-4
70-71	6.180669909076623E-4
72-73	0.006559990594267008
74-75	0.005486077534931812
76-77	0.004429458696648815
78-79	0.0025256756271234797
80-81	2.3939592746830066E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0018379339635294293
90-91	2.1321421431439965E-4
92-93	0.0
94-95	1.0240448285864162E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	113.0
22-23	246.0
24-25	357.0
26-27	964.0
28-29	2671.0
30-31	8769.0
32-33	11141.0
34-35	15480.0
36-37	24656.0
38-39	28422.0
40-41	28309.0
42-43	31717.0
44-45	37715.0
46-47	38618.0
48-49	36990.0
50-51	32931.0
52-53	31106.0
54-55	33688.0
56-57	35257.0
58-59	20069.0
60-61	36003.0
62-63	35423.0
64-65	37366.0
66-67	39629.0
68-69	40191.0
70-71	40054.0
72-73	36784.0
74-75	36630.0
76-77	38481.0
78-79	39469.0
80-81	39342.0
82-83	37097.0
84-85	36679.0
86-87	37823.0
88-89	39460.0
90-91	40035.0
92-93	39735.0
94-95	46960.0
96-97	170099.0
98-99	341439.0
100-101	3358416.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.36755665901076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.51023849801896	80.86536185353279
2	6.339517347011037	11.204153167055587
3	1.3007767337365992	3.448393851575756
4	0.42767858324524166	1.5117164574707738
5	0.1768307245859663	0.7813049536952152
6	0.08155106468209479	0.4323880997338598
7	0.04731469809934398	0.29267589855684367
8	0.030362030136684953	0.21464147347088802
9	0.018568794197255753	0.14767910759839573
>10	0.06496819134249195	0.8856693375245589
>50	0.0016087807875793776	0.09790928505661464
>100	5.20690772263874E-4	0.08097260559896743
>500	6.386338435808667E-5	0.03713390912976554
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.005467707156792E-5	0.0	0.0	0.0
3	0.0	2.005467707156792E-5	0.0	0.0	0.0
4	0.0	2.005467707156792E-5	0.0	2.005467707156792E-5	0.0
5	0.0	6.016403121470377E-5	0.0	2.005467707156792E-5	0.0
6	4.010935414313584E-5	6.016403121470377E-5	0.0	2.005467707156792E-5	0.0
7	4.010935414313584E-5	6.016403121470377E-5	0.0	4.010935414313584E-5	0.0
8	4.010935414313584E-5	6.016403121470377E-5	0.0	4.010935414313584E-5	0.0
9	4.010935414313584E-5	1.0027338535783961E-4	0.0	4.010935414313584E-5	0.0
10-11	4.010935414313584E-5	1.0027338535783961E-4	0.0	8.021870828627168E-5	0.0
12-13	4.010935414313584E-5	1.0027338535783961E-4	0.0	1.0027338535783961E-4	0.0
14-15	4.010935414313584E-5	1.7046475510832732E-4	0.0	1.0027338535783961E-4	1.002733853578396E-5
16-17	6.016403121470377E-5	1.804920936441113E-4	0.0	1.2032806242940754E-4	2.005467707156792E-5
18-19	6.016403121470377E-5	2.1057410925146318E-4	0.0	1.4038273950097547E-4	2.005467707156792E-5
20-21	6.016403121470377E-5	2.2060144778724715E-4	0.0	1.7046475510832735E-4	2.005467707156792E-5
22-23	6.016403121470377E-5	2.2060144778724715E-4	0.0	2.4065612485881508E-4	3.0082015607351882E-5
24-25	6.016403121470377E-5	2.2060144778724715E-4	0.0	2.8076547900195094E-4	4.010935414313584E-5
26-27	6.016403121470377E-5	2.2060144778724715E-4	0.0	2.907928175377349E-4	4.010935414313584E-5
28-29	6.016403121470377E-5	2.2060144778724715E-4	0.0	5.715582965396858E-4	5.0136692678919806E-5
30-31	6.016403121470377E-5	2.8076547900195094E-4	0.0	0.001313581348187699	6.016403121470377E-5
32-33	6.016403121470377E-5	2.8076547900195094E-4	0.0	0.003158611638771948	6.016403121470377E-5
34-35	6.016403121470377E-5	3.3090217168087074E-4	0.0	0.005284407408358147	8.021870828627168E-5
36-37	6.016403121470377E-5	3.8103886435979055E-4	0.0	0.008773921218810966	8.021870828627168E-5
38-39	6.016403121470377E-5	3.8103886435979055E-4	0.0	0.018711013707772874	8.021870828627168E-5
40-41	6.016403121470377E-5	3.8103886435979055E-4	0.0	0.02898903570695143	8.021870828627168E-5
42-43	6.016403121470377E-5	4.0109354143135845E-4	0.0	0.03490516544306397	9.024604682205565E-5
44-45	6.016403121470377E-5	4.712849111818462E-4	0.0	0.04231536862100832	1.1030072389362358E-4
46-47	6.016403121470377E-5	5.21421603860766E-4	0.0	0.05019685671013451	1.2032806242940754E-4
48-49	6.016403121470377E-5	5.21421603860766E-4	0.0	0.05786777069000924	1.4038273950097547E-4
50-51	6.016403121470377E-5	5.615309580039019E-4	0.0	0.06626065304446041	1.4038273950097547E-4
52-53	6.016403121470377E-5	5.615309580039019E-4	0.0	0.08960429715576548	1.4038273950097547E-4
54-55	6.016403121470377E-5	5.615309580039019E-4	0.0	0.11893426237293356	1.4038273950097547E-4
56-57	6.016403121470377E-5	5.815856350754698E-4	0.0	0.1546616695759318	1.4038273950097547E-4
58-59	6.016403121470377E-5	5.815856350754698E-4	0.0	0.3413606857736934	1.4038273950097547E-4
60-61	6.016403121470377E-5	5.815856350754698E-4	0.0	0.5137807718964986	1.4038273950097547E-4
62-63	6.016403121470377E-5	5.815856350754698E-4	0.0	0.5198573390491836	1.6043741657254337E-4
64-65	6.016403121470377E-5	6.216949892186056E-4	0.0	0.5258035508009036	1.6043741657254337E-4
66-67	6.016403121470377E-5	6.216949892186056E-4	0.0	0.5311581495790122	1.6043741657254337E-4
68-69	6.016403121470377E-5	6.417496662901735E-4	0.0	0.536322228924941	1.6043741657254337E-4
70-71	6.016403121470377E-5	6.417496662901735E-4	0.0	0.5411854881147962	1.6043741657254337E-4
72-73	6.016403121470377E-5	6.417496662901735E-4	0.0	0.544765247972071	1.804920936441113E-4
74-75	6.016403121470377E-5	6.417496662901735E-4	0.0	0.5476130121162337	1.804920936441113E-4
76-77	6.016403121470377E-5	6.517770048259574E-4	0.0	0.5507916784320772	1.804920936441113E-4
78-79	6.016403121470377E-5	6.618043433617415E-4	0.0	0.552656763399733	1.804920936441113E-4
80-81	6.016403121470377E-5	6.618043433617415E-4	0.0	0.5532383490348085	2.0054677071567923E-4
82-83	6.016403121470377E-5	6.618043433617415E-4	0.0	0.5538299620084197	2.2060144778724715E-4
84-85	6.016403121470377E-5	6.618043433617415E-4	0.0	0.5540806454718143	2.2060144778724715E-4
86-87	6.016403121470377E-5	6.618043433617415E-4	0.0	0.5542110008727795	2.2060144778724715E-4
88	6.016403121470377E-5	7.019136975048773E-4	0.0	0.5542110008727795	2.2060144778724715E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6300	0.0	47.65203	1
GTATCAA	11195	0.0	37.43501	1
TCAACGC	13535	0.0	30.994877	4
ATCAACG	13575	0.0	30.84009	3
CAACGCA	13960	0.0	30.112972	5
TATCAAC	14220	0.0	29.68354	2
AACGCAG	14440	0.0	29.198542	6
CGAGACC	3510	0.0	27.245346	86-87
ACGAGAC	3645	0.0	26.012653	86-87
GAGACCG	3715	0.0	25.710403	88-89
ACGCAGA	16485	0.0	25.654774	7
AGACCGA	3805	0.0	25.53507	88-89
GACCGAG	3875	0.0	25.310783	90-91
CGCAGAG	16705	0.0	25.188005	8
ACCGAGG	4035	0.0	24.652405	90-91
TCTCCGA	3640	0.0	24.649162	74-75
CCCACGA	3840	0.0	24.640957	82-83
ATCTCCG	3590	0.0	24.419575	74-75
CACGAGA	3930	0.0	24.234735	84-85
TGGTATC	2970	0.0	24.21861	2
>>END_MODULE