FastQCFastQC Report
Fri 27 May 2016
SRR2138863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254126
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56200.053.10311
ATCTCGT39600.040.95654794
GTATCAA108000.040.1975361
ATCAACG127350.033.644393
TCAACGC128100.033.515534
CAACGCA131050.032.7610785
TATCAAC132950.032.5554282
TATCTCG46550.032.11965694
AACGCAG136300.031.5286056
TATGCCG21050.028.38685486-87
CGCAGAG155200.027.7172138
ACGCAGA155550.027.6828967
ATGCCGT21850.027.6309588-89
GCCGTCT22700.026.98083590-91
TGCCGTC22500.026.71239788-89
CCGTCTT23250.026.46070990-91
TCTCGTA23550.024.98380582-83
CGTATGC24000.024.87261684-85
CTCGTAT23800.024.50064782-83
TCGTATG24050.024.37964684-85
Produced by FastQC (version 0.11.5)