FastQCFastQC Report
Fri 27 May 2016
SRR2138862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904403
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73550.048.826591
GTATCAA142300.038.8143121
ATCAACG170000.032.088443
TCAACGC170550.032.0099684
CAACGCA174600.031.2674675
TATCAAC176350.031.199042
AACGCAG181650.030.0516596
ACGCAGA209550.026.070867
CGCAGAG213550.025.6024998
TGGTATC34050.022.7971882
GTGGTAT38250.022.0780531
GCAGAGT251350.021.6503899
GTACATG196400.020.0658471
TACATGG193050.019.994282
GAGTACT140650.019.49688112-13
ACATGGG198150.018.9415513
AGAGTAC226900.018.85212510-11
CAGAGTA245300.018.44640410-11
TATACCG3652.910383E-1117.5277185
GTACTTT159200.017.22517614-15
Produced by FastQC (version 0.11.5)