##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5904403
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.810561541954367	30.0	18.0	32.0	18.0	33.0
2	28.812053818142157	32.0	27.0	32.0	18.0	33.0
3	31.220936138674816	33.0	30.0	33.0	27.0	33.0
4	31.70822824932512	33.0	31.0	33.0	29.0	33.0
5	32.90933562631142	33.0	33.0	33.0	32.0	34.0
6	36.98520206022523	38.0	37.0	38.0	35.0	38.0
7	37.377929487536676	38.0	38.0	38.0	37.0	38.0
8	37.50295449006445	38.0	38.0	38.0	37.0	38.0
9	37.499993310077244	38.0	38.0	38.0	37.0	38.0
10-11	37.533001050233196	38.0	38.0	38.0	37.0	38.0
12-13	37.545817333268076	38.0	38.0	38.0	37.5	38.0
14-15	37.56705741461076	38.0	38.0	38.0	38.0	38.0
16-17	37.57090810027703	38.0	38.0	38.0	38.0	38.0
18-19	37.56625868864303	38.0	38.0	38.0	38.0	38.0
20-21	37.56270885994206	38.0	38.0	38.0	38.0	38.0
22-23	37.562888796985774	38.0	38.0	38.0	38.0	38.0
24-25	37.526306448724114	38.0	38.0	38.0	38.0	38.0
26-27	37.530913090429294	38.0	38.0	38.0	38.0	38.0
28-29	37.52256169919528	38.0	38.0	38.0	38.0	38.0
30-31	37.515821827769486	38.0	38.0	38.0	38.0	38.0
32-33	37.51246354878694	38.0	38.0	38.0	38.0	38.0
34-35	37.49545762756043	38.0	38.0	38.0	37.5	38.0
36-37	37.47278720700024	38.0	38.0	38.0	37.0	38.0
38-39	37.45295945968293	38.0	38.0	38.0	37.0	38.0
40-41	37.43212060329708	38.0	38.0	38.0	37.0	38.0
42-43	37.41865678143377	38.0	38.0	38.0	37.0	38.0
44-45	37.38747991776617	38.0	38.0	38.0	37.0	38.0
46-47	37.34994795204834	38.0	38.0	38.0	37.0	38.0
48-49	37.30553770403212	38.0	38.0	38.0	37.0	38.0
50-51	37.28553928076101	38.0	38.0	38.0	37.0	38.0
52-53	37.27415322315747	38.0	38.0	38.0	37.0	38.0
54-55	37.25285064509687	38.0	38.0	38.0	37.0	38.0
56-57	37.22406482031573	38.0	38.0	38.0	37.0	38.0
58-59	37.2275689664378	38.0	38.0	38.0	37.0	38.0
60-61	37.24360197686265	38.0	38.0	38.0	37.0	38.0
62-63	37.25139915857765	38.0	38.0	38.0	37.0	38.0
64-65	37.260728166241414	38.0	38.0	38.0	37.0	38.0
66-67	37.26916924494079	38.0	38.0	38.0	37.0	38.0
68-69	37.27040660446177	38.0	38.0	38.0	37.0	38.0
70-71	37.24115465886949	38.0	38.0	38.0	37.0	38.0
72-73	37.22250514397375	38.0	38.0	38.0	37.0	38.0
74-75	37.208701453714866	38.0	38.0	38.0	37.0	38.0
76-77	37.21134390193522	38.0	38.0	38.0	37.0	38.0
78-79	37.217524358720695	38.0	38.0	38.0	37.0	38.0
80-81	37.20694520050797	38.0	38.0	38.0	37.0	38.0
82-83	37.20812718907919	38.0	38.0	38.0	36.5	38.0
84-85	37.20398297148479	38.0	38.0	38.0	36.5	38.0
86-87	37.17671354560754	38.0	38.0	38.0	36.0	38.0
88-89	37.13822127695043	38.0	38.0	38.0	36.0	38.0
90-91	37.14627487186986	38.0	38.0	38.0	36.0	38.0
92-93	37.12595245290394	38.0	38.0	38.0	36.0	38.0
94-95	37.111118183220626	38.0	38.0	38.0	36.0	38.0
96-97	37.10372533784894	38.0	38.0	38.0	36.0	38.0
98-99	37.14199606966963	38.0	38.0	38.0	36.0	38.0
100	35.551956587588535	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	21.0
20	54.0
21	153.0
22	337.0
23	893.0
24	2010.0
25	3819.0
26	6652.0
27	10889.0
28	17307.0
29	25981.0
30	36719.0
31	49298.0
32	64605.0
33	83536.0
34	123970.0
35	234309.0
36	829285.0
37	4414559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.515374712735564	21.16972706639435	14.256547190291718	24.05835103057837
2	14.119530797609853	21.395067375990426	42.003958740621194	22.48144308577853
3	18.39826990129231	26.140390484863584	30.250086249194037	25.211253364650076
4	12.511561287398573	17.94533672582986	39.05084053375083	30.492261453020735
5	13.223383790674445	38.005426675264516	35.26481846688886	13.506371067172172
6	29.80310609142541	37.962989622312044	18.83856745429424	13.395336831968304
7	26.341155913646137	32.88681345091112	23.145727010842588	17.62630362460015
8	24.09021269215748	37.12826464051736	21.17650525828018	17.60501740904498
9	25.458717072448657	17.14392075607318	21.484682106672278	35.91268006480588
10-11	24.091817580879894	27.069713906723507	28.847802224882006	19.99066628751459
12-13	24.967909541404946	24.565396366067834	29.0761487655907	21.390545326936525
14-15	22.036359984235492	25.83620223755052	26.96005845129474	25.16737932691925
16-17	21.46707296232998	29.005760616272298	27.632446497977863	21.89471992341986
18-19	21.50844039609085	28.07133083564926	29.481126203614487	20.939102564645403
20-21	22.396837315664555	27.202437050029964	29.367848441949974	21.032877192355503
22-23	22.09115760555675	27.099084206586195	29.221905873306493	21.587852314550563
24-25	21.80224945806293	27.154494156497265	29.36566346343098	21.677592922008827
26-27	21.741749066948877	27.34226640054647	29.28939893848363	21.62658559402102
28-29	21.625252250876787	27.469703206053786	29.32819633146015	21.576848211609274
30-31	21.954247107685447	27.27750529441349	29.248833617596198	21.519413980304865
32-33	21.723483557520247	27.442931393585173	29.179479796150343	21.654105252744237
34-35	21.80326964882626	27.440622249017732	29.226767998694996	21.52934010346101
36-37	21.807610862489394	27.34800760085011	29.23521259403185	21.609168942628642
38-39	21.663497206886667	27.456859333664486	29.2774234315048	21.602220027944046
40-41	21.904214125483897	27.36245503086352	29.13409972176518	21.599231121887406
42-43	21.90352153900576	27.37435434614094	28.971830897471946	21.75029321738135
44-45	21.868604819177992	27.51532206662361	28.866481720219927	21.74959139397847
46-47	21.900472986689273	27.499307587501825	28.690212741578353	21.910006684230545
48-49	21.898177065927676	27.572547786959987	28.659309506355534	21.869965640756803
50-51	21.85860626635708	27.7150427469805	28.528261260680882	21.898089725981542
52-53	21.986122620735582	27.628126897496845	28.42195910872135	21.96379137304622
54-55	21.97575047388271	27.57975368545404	28.485835016793676	21.95866082386958
56-57	22.001389368678005	27.662239836372688	28.376021296385005	21.960349498564298
58-59	21.929547690038163	27.683654674556102	28.473390393358187	21.913407242047544
60-61	21.990536618053678	27.641218394443957	28.455134672506293	21.91311031499607
62-63	21.951622368086323	27.70422573004454	28.422586468443868	21.921565433425265
64-65	21.916238217959464	27.646857448177958	28.46803844861442	21.968865885248153
66-67	22.028062944991927	27.70219611503141	28.253988760415993	22.01575217956067
68-69	21.995561708363905	27.66210706120575	28.31774782249486	22.024583407935484
70-71	21.99483048959035	27.478465791447555	28.33033758648606	22.196366132476044
72-73	22.032505440293093	27.498300996715425	28.301464376652362	22.16772918633912
74-75	21.97054178871117	27.638016879378903	28.226562408193555	22.164878923716373
76-77	22.070121365266704	27.53233423648605	28.159084697063513	22.238459701183732
78-79	22.087444643194896	27.621311604073252	28.069739891920648	22.221503860811204
80-81	22.0881967437151	27.65344978599959	28.02267555384383	22.235677916441485
82-83	22.121940863253766	27.65239208124914	28.109605199553073	22.11606185594403
84-85	22.23848800358711	27.638627633862704	28.016419658286175	22.106464704264006
86-87	22.099543262048062	27.649763093866053	28.080718828701922	22.16997481538396
88-89	22.225345049327426	27.633561523798228	28.00229718325429	22.13879624362006
90-91	22.252117182088508	27.635183410612356	28.03444230829407	22.078257099005064
92-93	22.24630293619004	27.648855572703052	28.004202257274834	22.100639233832073
94-95	22.12558916422697	27.7564080056669	27.97694227040499	22.14106055970114
96-97	22.2436856806214	27.825042798918503	28.050186451686898	21.881085068773203
98-99	23.247656053735184	29.790424706743117	28.293012995538568	18.668906243983123
100	22.10777053807931	30.64171226387734	22.25551194550247	24.995005252540874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1205.0
1	1136.5
2	1561.5
3	2266.0
4	2785.5
5	3552.0
6	4375.5
7	5915.5
8	7864.5
9	9136.0
10	9403.0
11	9414.0
12	10372.0
13	12374.0
14	15984.5
15	19821.0
16	22038.0
17	22674.5
18	21749.0
19	19969.5
20	18458.5
21	17860.0
22	19182.0
23	22837.5
24	29141.0
25	38358.0
26	49900.5
27	62710.5
28	75887.5
29	89910.5
30	103555.0
31	116566.5
32	131004.0
33	145413.5
34	159719.0
35	173355.5
36	186335.0
37	198218.5
38	206551.0
39	213763.5
40	222705.0
41	229991.0
42	233969.0
43	239078.5
44	243215.5
45	246307.0
46	247994.0
47	247346.5
48	246081.0
49	242189.5
50	236528.0
51	228161.0
52	217497.5
53	206043.5
54	193646.5
55	179283.0
56	163164.0
57	146137.5
58	129532.0
59	113363.0
60	96061.0
61	78794.0
62	62616.0
63	48878.0
64	37110.5
65	27193.0
66	19822.0
67	14999.5
68	11312.0
69	8008.0
70	5660.0
71	4023.0
72	2869.5
73	1984.5
74	1317.5
75	894.0
76	552.5
77	323.5
78	212.5
79	124.5
80	68.5
81	50.0
82	41.5
83	33.5
84	21.5
85	11.5
86	4.5
87	2.0
88	2.0
89	2.5
90	3.5
91	4.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.01022965403953626
6	8.468256655245247E-5
7	0.0
8	1.6936513310490493E-5
9	1.6936513310490493E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.436466989393379E-4
26-27	0.0
28-29	7.286394268488379E-4
30-31	0.0024170947115239867
32-33	2.294756744311319E-4
34-35	6.818191309414038E-5
36-37	3.080722371204625E-4
38-39	5.595664995284576E-4
40-41	5.80537036616637E-4
42-43	1.1337958464522961E-4
44-45	0.0
46-47	0.0014012121904396356
48-49	8.052731432817136E-5
50-51	0.001867669903149135
52-53	0.001491152284290729
54-55	0.002703115537663883
56-57	0.0022819938306712535
58-59	0.0035317631307039375
60-61	0.002839009189553123
62-63	0.002815980509243053
64-65	0.004275456469540386
66-67	0.0016607188151041604
68-69	1.0727095189414658E-4
70-71	6.403232430539952E-4
72-73	0.006735857956774497
74-75	0.005764798433421047
76-77	0.004665141706214729
78-79	0.0027253729765130226
80-81	3.105955638877992E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020706575211188196
90-91	2.289635886837036E-4
92-93	0.0
94-95	1.2261177345000327E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	146.0
22-23	290.0
24-25	405.0
26-27	1399.0
28-29	3839.0
30-31	11870.0
32-33	15200.0
34-35	20833.0
36-37	32907.0
38-39	37893.0
40-41	36736.0
42-43	41825.0
44-45	50122.0
46-47	50589.0
48-49	47541.0
50-51	43414.0
52-53	41741.0
54-55	44747.0
56-57	45473.0
58-59	46976.0
60-61	45630.0
62-63	45666.0
64-65	47886.0
66-67	51096.0
68-69	51684.0
70-71	51133.0
72-73	47120.0
74-75	47847.0
76-77	49569.0
78-79	50616.0
80-81	50430.0
82-83	47635.0
84-85	46833.0
86-87	48588.0
88-89	50131.0
90-91	50036.0
92-93	49594.0
94-95	57174.0
96-97	176876.0
98-99	385811.0
100-101	3879075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.10785724639499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.05380661846391	77.54340324797153
2	7.063660065067927	12.164732650398603
3	1.6947232299987958	4.377869578826572
4	0.5799377915253565	1.997488022578199
5	0.24900141365973988	1.0720489090781713
6	0.12849885717905354	0.6638856750179304
7	0.07228571853472303	0.43570578327787135
8	0.04248845556412208	0.29268718930681764
9	0.028751964033109374	0.22281930130648187
>10	0.08532233248772525	1.0733611598134587
>50	0.0010041166643931627	0.05946924768561744
>100	4.6054832854775044E-4	0.06009581040323364
>500	5.8888492648830065E-5	0.03643342433550533
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.3873026620980986E-5	0.0
5	0.0	0.0	0.0	3.3873026620980986E-5	0.0
6	0.0	0.0	0.0	3.3873026620980986E-5	0.0
7	0.0	0.0	0.0	5.080953993147148E-5	0.0
8	0.0	1.6936513310490493E-5	0.0	5.080953993147148E-5	0.0
9	0.0	3.3873026620980986E-5	0.0	5.080953993147148E-5	0.0
10-11	0.0	3.3873026620980986E-5	0.0	5.927779658671672E-5	0.0
12-13	0.0	3.3873026620980986E-5	0.0	7.621430989720722E-5	0.0
14-15	0.0	2.2864292969162169E-4	0.0	8.468256655245247E-5	0.0
16-17	0.0	2.540476996573574E-4	0.0	8.468256655245247E-5	0.0
18-19	0.0	2.7098421296784794E-4	0.0	9.315082320769772E-5	0.0
20-21	0.0	2.9638898293358367E-4	0.0	1.3549210648392394E-4	0.0
22-23	0.0	3.2179375289931935E-4	0.0	1.608968764496597E-4	0.0
24-25	0.0	3.4719852286505513E-4	0.0	1.6936513310490493E-4	0.0
26-27	0.0	3.5566677952030034E-4	0.0	1.947699030706407E-4	0.0
28-29	0.0	3.5566677952030034E-4	0.0	3.895398061412814E-4	0.0
30-31	0.0	3.7260329283079086E-4	0.0	0.001405730604770711	0.0
32-33	0.0	3.7260329283079086E-4	0.0	0.004039358424551983	0.0
34-35	0.0	4.149445761070171E-4	0.0	0.007181081643647969	0.0
36-37	0.0	4.572858593832433E-4	0.0	0.012524551593107721	0.0
38-39	0.0	5.250319126252052E-4	0.0	0.02362643606813424	0.0
40-41	0.0	5.927779658671672E-4	0.0	0.03520254291585449	0.0
42-43	0.0	6.097144791776578E-4	0.0	0.04297640252536963	0.0
44-45	0.0	7.113335590406007E-4	0.0	0.05297741363521426	0.0
46-47	0.0	8.298891522140341E-4	0.0	0.06368975830409951	0.0
48-49	0.0	8.383574088692794E-4	0.0	0.07423273783987983	0.0
50-51	0.0	9.738495153532034E-4	0.0	0.08391195519682515	0.0
52-53	0.0	9.823177720084486E-4	0.0	0.10807189143423984	0.0
54-55	0.0	9.907860286636938E-4	0.0	0.13652523379586387	0.0
56-57	0.0	0.0010161907986294297	0.0	0.1803992715266895	0.0
58-59	0.0	0.0010161907986294297	0.0	0.21263792461320813	0.0
60-61	0.0	0.0010161907986294297	0.0	0.22656820681108658	0.0
62-63	0.0	0.0010670003385609011	0.0	0.23711965460352213	0.0
64-65	0.0	0.001168619418423844	0.0	0.24242078326970568	0.0
66-67	0.0	0.001168619418423844	0.0	0.24778118973247593	0.0
68-69	0.0	0.001227897215010561	0.0	0.25304844537203847	0.0
70-71	0.0	0.0012533019849762966	0.0	0.2580531850552884	0.0
72-73	0.0	0.0012533019849762966	0.0	0.2623719959494635	0.0
74-75	0.0	0.0012617702416315418	0.0	0.265496782655249	0.0
76-77	0.0	0.0013464528081839943	0.0	0.2683590534047219	0.0
78-79	0.0	0.0014311353747364468	0.0	0.270298284178773	0.0
80-81	0.0	0.001439603631391692	0.0	0.27086565737467444	0.0
82-83	0.0	0.001439603631391692	0.0	0.2714669035971969	0.0
84-85	0.0	0.0014565401447021824	0.0	0.27162780047364654	0.0
86-87	0.0	0.0014904131713231634	0.0	0.2717378878101647	0.0
88	0.0	0.0016089687644965968	0.0	0.27174635606681996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7355	0.0	48.82659	1
GTATCAA	14230	0.0	38.814312	1
ATCAACG	17000	0.0	32.08844	3
TCAACGC	17055	0.0	32.009968	4
CAACGCA	17460	0.0	31.267467	5
TATCAAC	17635	0.0	31.19904	2
AACGCAG	18165	0.0	30.051659	6
ACGCAGA	20955	0.0	26.07086	7
CGCAGAG	21355	0.0	25.602499	8
TGGTATC	3405	0.0	22.797188	2
GTGGTAT	3825	0.0	22.078053	1
GCAGAGT	25135	0.0	21.650389	9
GTACATG	19640	0.0	20.065847	1
TACATGG	19305	0.0	19.99428	2
GAGTACT	14065	0.0	19.496881	12-13
ACATGGG	19815	0.0	18.941551	3
AGAGTAC	22690	0.0	18.852125	10-11
CAGAGTA	24530	0.0	18.446404	10-11
TATACCG	365	2.910383E-11	17.527718	5
GTACTTT	15920	0.0	17.225176	14-15
>>END_MODULE