FastQCFastQC Report
Fri 27 May 2016
SRR2138861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5283381
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.047.307751
GTATCAA106100.034.9942251
TCAACGC126450.030.84924
ATCAACG128200.030.4280913
CAACGCA129250.030.18095
TATCAAC134500.029.4158022
AACGCAG135350.028.9118426
ACGCAGA156700.024.8908847
CGCAGAG157350.024.815218
GCAGAGT191950.020.3643959
GAGTACT112500.019.82241212-13
TACATGG134200.019.4527342
ACATGGG136700.018.5656383
GTACATG133450.018.5375331
CAGAGTA186000.018.23669210-11
AGAGTAC169500.017.92001710-11
CATGGGG104700.017.6698634
GTACTTT127600.017.44317614-15
TGGTATC29300.017.3528692
GTGGTAT28850.016.8830551
Produced by FastQC (version 0.11.5)