##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5283381
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.202209532115894	18.0	18.0	32.0	18.0	33.0
2	30.436175623147374	32.0	27.0	33.0	27.0	33.0
3	30.96326708219604	33.0	30.0	33.0	27.0	33.0
4	32.29700924464846	33.0	33.0	33.0	31.0	33.0
5	32.7719901328335	33.0	33.0	33.0	32.0	34.0
6	37.14895726808269	38.0	37.0	38.0	36.0	38.0
7	37.14219417452574	38.0	38.0	38.0	36.0	38.0
8	37.44983846517978	38.0	38.0	38.0	37.0	38.0
9	37.50344561560107	38.0	38.0	38.0	37.0	38.0
10-11	37.53100807986401	38.0	38.0	38.0	37.0	38.0
12-13	37.54889104155086	38.0	38.0	38.0	37.5	38.0
14-15	37.560820145281966	38.0	38.0	38.0	38.0	38.0
16-17	37.566195680379664	38.0	38.0	38.0	38.0	38.0
18-19	37.56261511331475	38.0	38.0	38.0	38.0	38.0
20-21	37.55602625224327	38.0	38.0	38.0	38.0	38.0
22-23	37.564722465768	38.0	38.0	38.0	38.0	38.0
24-25	37.5311191758629	38.0	38.0	38.0	38.0	38.0
26-27	37.52991691386552	38.0	38.0	38.0	38.0	38.0
28-29	37.518614720596474	38.0	38.0	38.0	37.5	38.0
30-31	37.51190020282499	38.0	38.0	38.0	37.5	38.0
32-33	37.50830407911252	38.0	38.0	38.0	38.0	38.0
34-35	37.49461823871437	38.0	38.0	38.0	37.5	38.0
36-37	37.47180109372417	38.0	38.0	38.0	37.0	38.0
38-39	37.4523836555383	38.0	38.0	38.0	37.0	38.0
40-41	37.4324830537925	38.0	38.0	38.0	37.0	38.0
42-43	37.41340921900217	38.0	38.0	38.0	37.0	38.0
44-45	37.38746601959811	38.0	38.0	38.0	37.0	38.0
46-47	37.35643634728382	38.0	38.0	38.0	37.0	38.0
48-49	37.31775953186915	38.0	38.0	38.0	37.0	38.0
50-51	37.29985156624761	38.0	38.0	38.0	37.0	38.0
52-53	37.2870834800335	38.0	38.0	38.0	37.0	38.0
54-55	37.263821380388066	38.0	38.0	38.0	37.0	38.0
56-57	37.23177544672623	38.0	38.0	38.0	37.0	38.0
58-59	37.235766382219246	38.0	38.0	38.0	37.0	38.0
60-61	37.250604411624224	38.0	38.0	38.0	37.0	38.0
62-63	37.254808440013996	38.0	38.0	38.0	37.0	38.0
64-65	37.260719525194965	38.0	38.0	38.0	37.0	38.0
66-67	37.2665919431627	38.0	38.0	38.0	37.0	38.0
68-69	37.26468461274827	38.0	38.0	38.0	37.0	38.0
70-71	37.232894972464585	38.0	38.0	38.0	37.0	38.0
72-73	37.21187503984348	38.0	38.0	38.0	37.0	38.0
74-75	37.19467032432965	38.0	38.0	38.0	36.5	38.0
76-77	37.196402914092246	38.0	38.0	38.0	36.0	38.0
78-79	37.19993617852084	38.0	38.0	38.0	36.0	38.0
80-81	37.189031028689115	38.0	38.0	38.0	36.0	38.0
82-83	37.190605440431355	38.0	38.0	38.0	36.0	38.0
84-85	37.18078948641627	38.0	38.0	38.0	36.0	38.0
86-87	37.15371201684058	38.0	38.0	38.0	36.0	38.0
88-89	37.11427543548237	38.0	38.0	38.0	36.0	38.0
90-91	37.11955164897195	38.0	38.0	38.0	36.0	38.0
92-93	37.09997072484765	38.0	38.0	38.0	36.0	38.0
94-95	37.080033923329964	38.0	38.0	38.0	36.0	38.0
96-97	37.07177431827026	38.0	38.0	38.0	36.0	38.0
98-99	37.10984802471765	38.0	38.0	38.0	36.0	38.0
100	35.47922482969523	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	10.0
19	16.0
20	50.0
21	142.0
22	326.0
23	792.0
24	1686.0
25	3309.0
26	5997.0
27	9635.0
28	15323.0
29	22873.0
30	32360.0
31	43182.0
32	56776.0
33	75792.0
34	113608.0
35	212127.0
36	761856.0
37	3927518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.16900579382786	23.567900933133537	14.091412298299138	24.17168097473947
2	14.057267495946252	20.915262404888082	41.87966001316203	23.147810086003638
3	17.796123353587408	25.980409135740924	30.87774665503018	25.345720855641492
4	12.520334990037629	18.045433407130776	39.19253599163112	30.24169561120048
5	13.283545756793123	37.45261075284449	35.666565206165465	13.59727828419692
6	29.66412984412822	38.19347875915063	18.921141594747755	13.221249801973395
7	26.097200295492712	32.71027878181747	23.569707946372954	17.622812976316858
8	23.791720451680554	37.27256036855195	21.3955649603095	17.540154219458
9	25.20007086375421	17.101615670883287	21.668901978999042	36.02941148636346
10-11	24.015864464049105	26.8964822806156	29.16842729763643	19.919225957698863
12-13	24.785757453418558	24.40132559056407	29.32454615709145	21.488370798925914
14-15	21.89182457218209	25.80255900530361	26.98390481398181	25.321711608532492
16-17	21.55380238525293	28.856001488440832	27.632249122294983	21.95794700401126
18-19	21.556499521802422	28.060355669977238	29.3979839803338	20.98516082788654
20-21	22.346580882377296	27.264511895387177	29.2321063829163	21.15680083931922
22-23	22.0708346295104	27.144772796682748	29.078055396533646	21.706337177273205
24-25	21.82354373103782	27.233675772551507	29.176790556067484	21.765989940343193
26-27	21.839125066316196	27.426638075176186	29.01813218563519	21.716104672872426
28-29	21.728084208780224	27.543077080827675	29.066566281058808	21.66227242933329
30-31	22.03377446591394	27.303721146388742	29.038121982155058	21.62438240554226
32-33	21.835573454920038	27.438245040155824	28.96053509190411	21.76564641302003
34-35	21.88410658035326	27.482694968466937	29.001973297693954	21.63122515348585
36-37	21.937861631036615	27.375781274042232	28.935701139156283	21.75065595576487
38-39	21.767022744797387	27.54645013486779	29.001082518016496	21.68544460231833
40-41	22.016030256909183	27.387190789040268	28.919432446909315	21.677346507141234
42-43	21.914560335935402	27.439768051279845	28.75713715707032	21.888534455714428
44-45	22.00587360565624	27.530976697833005	28.636456375628267	21.82669332088249
46-47	22.007167175121502	27.533046925981928	28.419752847023886	22.040033051872683
48-49	21.993307906170976	27.63189093064612	28.43302617161538	21.941774991567524
50-51	21.930364552036515	27.725839175629506	28.334858052226288	22.008938220107694
52-53	22.115630763347394	27.661786358760267	28.217440087546862	22.005142790345474
54-55	22.124813365902533	27.561136713000682	28.26153045271895	22.05251946837783
56-57	22.100562793982064	27.652822279302242	28.213834941909422	22.03277998480627
58-59	22.043089350700594	27.684160250852702	28.26683210208542	22.005918296361283
60-61	22.099816975055706	27.61215801677111	28.32194253154976	21.966082476623427
62-63	22.065548925364094	27.6683317472406	28.241088629848193	22.02503069754711
64-65	22.05815116696279	27.63296023027955	28.25125928803805	22.05762931471961
66-67	22.12566982131041	27.697869521962982	28.074122610790198	22.102338045936406
68-69	22.087650884175243	27.683322631904783	28.119328993305515	22.10969749061446
70-71	22.145781349011234	27.469786646262957	28.107479256666924	22.276952748058886
72-73	22.177267980105295	27.493568598701135	28.096380430134598	22.232782991058976
74-75	22.057793199377933	27.647284882239397	28.027265083488377	22.26765683489429
76-77	22.230626783082226	27.52575924673504	27.958515267853695	22.285098702329044
78-79	22.19022772783876	27.600296880997544	27.923491710062304	22.28598368110139
80-81	22.17982669977976	27.60642931430139	27.926111674984583	22.28763231093427
82-83	22.260130005578034	27.591458991464425	27.970686418588436	22.17772458436911
84-85	22.32059377168826	27.6084919889528	27.85838528359016	22.212528955768782
86-87	22.193221344661662	27.61932419356003	27.932909988368486	22.254544473409823
88-89	22.318994436115755	27.55492751648005	27.869584657356068	22.25649339004813
90-91	22.316398379402187	27.641796267289415	27.864959526135113	22.176845827173285
92-93	22.355905607961656	27.64849039899869	27.878193973807125	22.11741001923253
94-95	22.21836075053676	27.781596039389317	27.85710255713514	22.142940652938787
96-97	22.33289691156059	27.802536762673185	27.93495522758938	21.929611098176846
98-99	23.409965506524237	29.727170989705538	28.121877398715924	18.740986105054304
100	22.284919148931312	30.64230247542991	22.113551354262178	24.9592270213766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	668.0
1	812.0
2	1331.5
3	1853.0
4	2329.5
5	3009.5
6	3559.5
7	4586.5
8	5804.0
9	6470.5
10	6793.0
11	7081.0
12	7952.0
13	9651.0
14	12734.0
15	16021.5
16	17810.0
17	18157.5
18	17475.0
19	16358.0
20	15458.0
21	15310.0
22	16675.5
23	20226.0
24	26185.0
25	34459.0
26	44898.0
27	56461.5
28	68266.5
29	81297.0
30	93912.0
31	106129.5
32	119000.5
33	130927.5
34	142310.0
35	152975.0
36	163697.0
37	175087.5
38	183920.5
39	190264.0
40	198248.5
41	205768.0
42	209855.0
43	213354.0
44	216759.5
45	220423.0
46	222481.0
47	222525.0
48	221378.5
49	217672.0
50	212881.0
51	204960.5
52	194614.5
53	184329.5
54	173433.0
55	160586.0
56	146900.0
57	132690.0
58	117031.0
59	101070.5
60	84869.0
61	69101.5
62	54684.5
63	42143.0
64	31585.5
65	23269.0
66	17279.5
67	13303.5
68	10125.0
69	7303.0
70	5151.0
71	3583.5
72	2489.0
73	1633.5
74	1061.5
75	727.5
76	491.0
77	291.5
78	183.5
79	122.0
80	76.0
81	48.5
82	32.0
83	21.5
84	16.0
85	11.0
86	7.5
87	5.5
88	2.5
89	1.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.010163946154933745
6	0.0
7	3.7854548063067945E-5
8	1.8927274031533972E-5
9	5.6781822094601924E-5
10-11	9.463637015766986E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.205462934595819E-4
26-27	0.0
28-29	5.870099804004942E-4
30-31	0.0022838248437119907
32-33	2.3739433696758742E-4
34-35	4.7602083143321684E-5
36-37	3.3449952610974283E-4
38-39	6.920685293947457E-4
40-41	5.610838980046212E-4
42-43	2.044677767059988E-4
44-45	0.0
46-47	0.0013454843763541409
48-49	7.983404099557839E-5
50-51	0.0019419161802569102
52-53	0.0014294899519022254
54-55	0.002727418988250136
56-57	0.0025946870282328722
58-59	0.0036754384361985418
60-61	0.002816816752038553
62-63	0.003178420363093872
64-65	0.0043343807554346424
66-67	0.0016335200023986952
68-69	8.679758780823723E-5
70-71	6.792425875461529E-4
72-73	0.0068794503518252665
74-75	0.005837265954096098
76-77	0.004968132975627759
78-79	0.0024913901991439856
80-81	2.298136567454962E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0023909545407813163
90-91	2.1748402398607137E-4
92-93	0.0
94-95	1.3580290504636188E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	28.0
20-21	132.0
22-23	250.0
24-25	359.0
26-27	1064.0
28-29	2920.0
30-31	10237.0
32-33	12654.0
34-35	17606.0
36-37	28094.0
38-39	33269.0
40-41	32960.0
42-43	36162.0
44-45	42496.0
46-47	44158.0
48-49	41826.0
50-51	38287.0
52-53	36610.0
54-55	38629.0
56-57	39676.0
58-59	40763.0
60-61	40263.0
62-63	39728.0
64-65	41873.0
66-67	43889.0
68-69	44368.0
70-71	44008.0
72-73	41078.0
74-75	41169.0
76-77	42748.0
78-79	43572.0
80-81	43954.0
82-83	41833.0
84-85	41183.0
86-87	42237.0
88-89	43929.0
90-91	43900.0
92-93	43827.0
94-95	50912.0
96-97	159183.0
98-99	347388.0
100-101	3504159.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.10579582669747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.40924423488183	78.75169169169641
2	7.0334624871144475	12.253106947146538
3	1.5501731767545808	4.050872046912226
4	0.5213347130295146	1.8164510028207528
5	0.21342408446127747	0.9295237362791935
6	0.10121040199872137	0.5289607567223159
7	0.055967749588436304	0.3412580757971045
8	0.033660998406688285	0.2345654443628623
9	0.020731350407834092	0.1625238698252866
>10	0.05906389060897962	0.7492759951824153
>50	9.618489824234748E-4	0.0571352927958025
>100	7.339691243166634E-4	0.11041539236813148
>500	3.109464095001391E-5	0.014219748090955981
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7854548063067945E-5	0.0	0.0	0.0
3	0.0	3.7854548063067945E-5	0.0	0.0	0.0
4	0.0	1.1356364418920385E-4	0.0	1.8927274031533972E-5	0.0
5	0.0	1.1356364418920385E-4	0.0	1.8927274031533972E-5	0.0
6	0.0	1.1356364418920385E-4	0.0	1.8927274031533972E-5	0.0
7	0.0	1.1356364418920385E-4	0.0	1.8927274031533972E-5	0.0
8	0.0	1.324909182207378E-4	0.0	1.8927274031533972E-5	0.0
9	0.0	3.4069093256761156E-4	0.0	1.8927274031533972E-5	0.0
10-11	0.0	3.4069093256761156E-4	0.0	3.7854548063067945E-5	0.0
12-13	0.0	3.5961820659914553E-4	0.0	3.7854548063067945E-5	0.0
14-15	0.0	4.542545767568154E-4	0.0	4.731818507883493E-5	0.0
16-17	0.0	4.7318185078834937E-4	0.0	5.6781822094601924E-5	0.0
18-19	0.0	4.921091248198833E-4	0.0	9.463637015766988E-5	0.0
20-21	0.0	5.299636728829512E-4	0.0	1.1356364418920385E-4	0.0
22-23	0.0	5.583545839302522E-4	0.0	1.324909182207378E-4	0.0
24-25	0.0	5.772818579617862E-4	0.0	2.271272883784077E-4	0.0
26-27	0.0	5.867454949775531E-4	0.0	2.839091104730096E-4	0.0
28-29	0.0	5.867454949775531E-4	0.0	5.772818579617863E-4	0.0
30-31	0.0	7.003091391667571E-4	0.0	0.0017791637589641934	0.0
32-33	0.0	7.003091391667571E-4	0.0	0.003993654820653668	0.0
34-35	0.0	7.38163687229825E-4	0.0	0.006548836814910756	0.0
36-37	0.0	7.76018235292893E-4	0.0	0.010731764375879763	0.0
38-39	0.0	8.328000573874949E-4	0.0	0.021189083278302285	0.0
40-41	0.0	9.085091535136308E-4	0.0	0.03316058410324752	0.0
42-43	0.0	9.558273385924657E-4	0.0	0.04065578461973497	0.0
44-45	0.0	0.0011072455308447373	0.0	0.05061153076032185	0.0
46-47	0.0	0.0012113455380181742	0.0	0.06092689510750786	0.0
48-49	0.0	0.0012113455380181742	0.0	0.07081639578898435	0.0
50-51	0.0	0.0013343728192231454	0.0	0.0810939055881073	0.0
52-53	0.0	0.0013438364562389123	0.0	0.10197068884488927	0.0
54-55	0.0	0.0013438364562389123	0.0	0.128137645193485	0.0
56-57	0.0	0.0013438364562389123	0.0	0.1659827296195372	0.0
58-59	0.0	0.0013627637302704462	0.0	0.19549034983469865	0.0
60-61	0.0	0.0013722273672862133	0.0	0.20888139621200894	0.0
62-63	0.0	0.0014006182783335142	0.0	0.22022829699391355	0.0
64-65	0.0	0.0016466728407434558	0.0	0.22478977003551326	0.0
66-67	0.0	0.0016561364777592227	0.0	0.2299285249350747	0.0
68-69	0.0	0.0017223819368695917	0.0	0.23529440712301458	0.0
70-71	0.0	0.0017413092109011257	0.0	0.24026281655629228	0.0
72-73	0.0	0.0017413092109011257	0.0	0.24430378956202475	0.0
74-75	0.0	0.0017507728479168928	0.0	0.2473321534070702	0.0
76-77	0.0	0.0017980910329957276	0.0	0.2502090990598634	0.0
78-79	0.0	0.0018170183070272616	0.0	0.25195040827076454	0.0
80-81	0.0	0.0018264819440430287	0.0	0.2525844719508209	0.0
82-83	0.0	0.0018548728550903295	0.0	0.253067117438625	0.0
84-85	0.0	0.0018927274031533975	0.0	0.2532279992678931	0.0
86-87	0.0	0.0019211183142006983	0.0	0.25334156291208226	0.0
88	0.0	0.001968436499279533	0.0	0.25334156291208226	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5735	0.0	47.30775	1
GTATCAA	10610	0.0	34.994225	1
TCAACGC	12645	0.0	30.8492	4
ATCAACG	12820	0.0	30.428091	3
CAACGCA	12925	0.0	30.1809	5
TATCAAC	13450	0.0	29.415802	2
AACGCAG	13535	0.0	28.911842	6
ACGCAGA	15670	0.0	24.890884	7
CGCAGAG	15735	0.0	24.81521	8
GCAGAGT	19195	0.0	20.364395	9
GAGTACT	11250	0.0	19.822412	12-13
TACATGG	13420	0.0	19.452734	2
ACATGGG	13670	0.0	18.565638	3
GTACATG	13345	0.0	18.537533	1
CAGAGTA	18600	0.0	18.236692	10-11
AGAGTAC	16950	0.0	17.920017	10-11
CATGGGG	10470	0.0	17.669863	4
GTACTTT	12760	0.0	17.443176	14-15
TGGTATC	2930	0.0	17.352869	2
GTGGTAT	2885	0.0	16.883055	1
>>END_MODULE