FastQCFastQC Report
Fri 27 May 2016
SRR2138860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5377236
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.045.7990381
GTATCAA105800.036.2203451
TCAACGC127050.032.3263444
ATCAACG128150.032.0488663
CAACGCA130500.031.5046625
TATCAAC133400.031.1096152
AACGCAG135850.030.3240996
ACGCAGA161700.025.476377
CGCAGAG162250.025.3900098
GCAGAGT191450.021.4502079
GAGTACT111050.019.99866112-13
AGAGTAC172400.018.21415710-11
TACATGG140650.018.2045482
CAGAGTA189250.018.10188310-11
ACATGGG142850.017.112183
GTACTTT130850.017.0547314-15
GTACATG137150.016.9776211
GTGGTAT28450.016.7615451
AGTACTT124950.016.4675212-13
TGGTATC28850.016.0824172
Produced by FastQC (version 0.11.5)