FastQCFastQC Report
Fri 27 May 2016
SRR2138859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5545306
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63350.047.4809841
GTATCAA115550.035.1684041
TCAACGC139250.030.5444894
ATCAACG139500.030.520643
CAACGCA142850.029.8048975
TATCAAC146850.029.1984652
AACGCAG149700.028.5256356
ACGCAGA172400.024.6696747
CGCAGAG175600.024.244658
CGAGACC42150.023.58523286-87
GAGACCG43400.023.14229488-89
ACGAGAC43450.022.87957686-87
GACCGAG45750.022.6422790-91
AGACCGA44950.022.58848488-89
ACCGAGG45650.022.5088790-91
CCCACGA45050.021.70440582-83
CACGAGA46550.021.14868584-85
GCCCACG46150.021.01388282-83
ATCTCCG43700.020.86242574-75
TCTCCGA45350.020.66966474-75
Produced by FastQC (version 0.11.5)