FastQCFastQC Report
Fri 27 May 2016
SRR2138858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4322627
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.048.942181
ATCTCGT41450.045.86842394
GTATCAA97050.036.589631
TATGCCG19650.035.6024186-87
TATCTCG49050.033.01896394
GCCGTCT22050.032.84201490-91
CCGTCTT22000.032.79053590-91
ATGCCGT22200.032.24020888-89
TGCCGTC22100.032.1388788-89
ATCAACG116650.032.041933
TCAACGC117000.031.9089744
CAACGCA120050.031.0982915
TCTCGTA22400.031.04029382-83
TATCAAC122950.030.6119372
TCGTATG23050.030.48779584-85
CTCGTAT22750.030.33033482-83
CGTATGC23100.030.30613384-85
AACGCAG124400.029.9731836
ACGCAGA144050.025.884517
CGCAGAG146850.025.3614128
Produced by FastQC (version 0.11.5)