##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4322627
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.242069926459074	18.0	18.0	32.0	18.0	33.0
2	30.470228405087923	32.0	27.0	33.0	27.0	33.0
3	31.00033035466627	33.0	31.0	33.0	28.0	33.0
4	32.33398625419218	33.0	33.0	33.0	31.0	33.0
5	32.79066387176132	33.0	33.0	33.0	32.0	34.0
6	37.15285774136885	38.0	37.0	38.0	36.0	38.0
7	37.1445507558251	38.0	38.0	38.0	36.0	38.0
8	37.45207185352796	38.0	38.0	38.0	37.0	38.0
9	37.50318799193176	38.0	38.0	38.0	37.0	38.0
10-11	37.53378165638627	38.0	38.0	38.0	37.0	38.0
12-13	37.552087307093586	38.0	38.0	38.0	37.5	38.0
14-15	37.56332156348443	38.0	38.0	38.0	38.0	38.0
16-17	37.56809932941241	38.0	38.0	38.0	38.0	38.0
18-19	37.566745870046155	38.0	38.0	38.0	38.0	38.0
20-21	37.56101941409787	38.0	38.0	38.0	38.0	38.0
22-23	37.56886778362629	38.0	38.0	38.0	38.0	38.0
24-25	37.53567722984593	38.0	38.0	38.0	38.0	38.0
26-27	37.53547062781652	38.0	38.0	38.0	38.0	38.0
28-29	37.52638298213638	38.0	38.0	38.0	38.0	38.0
30-31	37.51783580419115	38.0	38.0	38.0	37.5	38.0
32-33	37.51684228267024	38.0	38.0	38.0	38.0	38.0
34-35	37.502177126101806	38.0	38.0	38.0	37.5	38.0
36-37	37.48053921569891	38.0	38.0	38.0	37.0	38.0
38-39	37.46007730578195	38.0	38.0	38.0	37.0	38.0
40-41	37.44242662035742	38.0	38.0	38.0	37.0	38.0
42-43	37.42292727220975	38.0	38.0	38.0	37.0	38.0
44-45	37.39223246086675	38.0	38.0	38.0	37.0	38.0
46-47	37.357802797441266	38.0	38.0	38.0	37.0	38.0
48-49	37.3138099364959	38.0	38.0	38.0	37.0	38.0
50-51	37.29542207126539	38.0	38.0	38.0	37.0	38.0
52-53	37.285596111335025	38.0	38.0	38.0	37.0	38.0
54-55	37.265573689052346	38.0	38.0	38.0	37.0	38.0
56-57	37.236846554465146	38.0	38.0	38.0	37.0	38.0
58-59	37.24091552521237	38.0	38.0	38.0	37.0	38.0
60-61	37.25284805149114	38.0	38.0	38.0	37.0	38.0
62-63	37.2554576525705	38.0	38.0	38.0	37.0	38.0
64-65	37.26146643436586	38.0	38.0	38.0	37.0	38.0
66-67	37.26897247833587	38.0	38.0	38.0	37.0	38.0
68-69	37.2660673768754	38.0	38.0	38.0	37.0	38.0
70-71	37.23664973298989	38.0	38.0	38.0	37.0	38.0
72-73	37.21457151155764	38.0	38.0	38.0	37.0	38.0
74-75	37.192732727735105	38.0	38.0	38.0	36.0	38.0
76-77	37.19223973278598	38.0	38.0	38.0	36.0	38.0
78-79	37.196739659626374	38.0	38.0	38.0	36.0	38.0
80-81	37.184624670107596	38.0	38.0	38.0	36.0	38.0
82-83	37.169582609335606	38.0	38.0	38.0	36.0	38.0
84-85	37.178274515665365	38.0	38.0	38.0	36.0	38.0
86-87	37.154121626858434	38.0	38.0	38.0	36.0	38.0
88-89	37.11141725792244	38.0	38.0	38.0	36.0	38.0
90-91	37.1184150000907	38.0	38.0	38.0	36.0	38.0
92-93	37.099257017708844	38.0	38.0	38.0	36.0	38.0
94-95	37.06105161368169	38.0	38.0	38.0	36.0	38.0
96-97	37.01244167195618	38.0	38.0	38.0	36.0	38.0
98-99	37.061715969082265	38.0	38.0	38.0	36.0	38.0
100	35.36472662514649	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	20.0
20	37.0
21	110.0
22	263.0
23	678.0
24	1424.0
25	2740.0
26	4682.0
27	7895.0
28	12390.0
29	18465.0
30	26226.0
31	34967.0
32	46207.0
33	61735.0
34	92780.0
35	173170.0
36	607956.0
37	3230879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.233231782432306	23.517920930952403	14.41560884156787	23.83323844504742
2	13.938746044939801	20.921559968047205	42.16540080835103	22.974293178661956
3	17.75059471936857	26.094687327867984	30.871990574250336	25.282727378513115
4	12.388924605338374	18.044952756737974	39.30919785584091	30.256924782082745
5	13.129706210752174	37.806804568214346	35.595160258145484	13.468328962888002
6	29.62027231607468	38.290260730519236	18.878625575576315	13.210841377829762
7	26.23099332882527	32.97013598443724	23.32565358981934	17.473217096918148
8	23.922119581448968	37.43980223137458	21.271925613752934	17.36615257342352
9	25.41348707938184	17.211297021764086	21.539036687421024	35.83617921143305
10-11	23.86840500792747	27.185323552705743	29.097494312774884	19.848777126591898
12-13	24.68871359939222	24.677944685026027	29.2845184190077	21.34882329657405
14-15	21.858305146384364	26.045538974332043	27.010646535081563	25.085509344202034
16-17	21.371876407564198	29.21726764765963	27.699220867310547	21.711635077465623
18-19	21.41098457026248	28.3518911069588	29.513048430965704	20.72407589181301
20-21	22.26240006995811	27.512451167836666	29.34180727210433	20.883341490100893
22-23	21.9742272119665	27.352002306091013	29.250490345094676	21.423280136847808
24-25	21.73107404865009	27.40459663050728	29.364893725563963	21.499435595278666
26-27	21.573075616225424	27.667066992465	29.25397449938933	21.505882891920248
28-29	21.509659824520007	27.76428090917602	29.284203317193796	21.441855949110177
30-31	21.82794190539813	27.554670604849186	29.218312548717112	21.399074941035572
32-33	21.594253047412685	27.68628094641733	29.158515605058245	21.560950401111747
34-35	21.693672580873425	27.7077490819572	29.201035079290595	21.39754325787878
36-37	21.70502238106383	27.63184169473769	29.199117914186974	21.464018010011507
38-39	21.506135187100764	27.728417125835144	29.17855439232223	21.586893294741863
40-41	21.828672095540636	27.576510097186752	29.213108065721922	21.381709741550694
42-43	21.636433799264328	27.58883142113898	29.072917010298386	21.701817769298298
44-45	21.686953081154225	27.69401890954819	28.984204986722435	21.634823022575148
46-47	21.666694394434	27.688912292731928	28.938325488786287	21.706067824047786
48-49	21.677375994109717	27.909782685211425	28.767635253814767	21.645206066864088
50-51	21.631628414593248	28.048153879040687	28.496115468114773	21.82410223825129
52-53	21.64890571045873	27.80766064121312	28.457254506801675	22.086179141526475
54-55	21.842562431204332	27.552835011770355	28.791762682905325	21.81283987411999
56-57	21.719147097176382	27.306128440926926	28.838512466478715	22.136211995417984
58-59	21.50810628243815	27.135326261414725	28.895253131319325	22.461314324827796
60-61	21.720062312476994	27.330936508939757	29.24191443559991	21.70708674298334
62-63	21.27262982915784	27.538974957036732	29.199975458488296	21.98841975531713
64-65	21.051189315575012	27.90517829843345	28.89192582769041	22.15170655830112
66-67	21.15425652588079	28.520906267654368	28.326094181628463	21.998743024836383
68-69	21.308926541033337	28.48879808225138	28.144587450075733	22.057687926639545
70-71	21.319476748101234	27.95115272685455	28.110634945137598	22.618735579906613
72-73	21.51568132130287	27.47943960967448	28.12179045166519	22.88308861735746
74-75	21.63655465149108	27.515178863537194	28.26655617982788	22.581710305143847
76-77	21.906596936231306	27.421258147538772	27.637958025195104	23.034186891034818
78-79	22.130513844889617	27.523132143098035	27.207170433637156	23.139183578375192
80-81	21.974136932116842	27.868197837571163	27.315189077517292	22.842476152794706
82-83	21.976874250551877	27.61901290968917	27.38714982824348	23.016963011515468
84-85	22.028863682160104	28.038551109595332	27.30550082295973	22.627084385284835
86-87	22.522821349690407	27.941870177306345	27.32906620966032	22.20624226334293
88-89	22.297177265175332	28.063249855908108	27.649401581570675	21.99017129734589
90-91	22.25537641339584	27.65644059218289	27.512239566009466	22.575943428411804
92-93	22.2187278523318	27.21518422541958	27.727398079705413	22.838689842543214
94-95	21.53538143187669	27.734347722860775	28.29992283842856	22.430348006833977
96-97	21.626240144410815	27.571416842100287	28.583468009649437	22.218875003839457
98-99	22.807945638483076	29.45125033093456	28.874405320625446	18.86639870995692
100	21.753853478748184	30.570698678549423	22.6065417610191	25.068906081683295
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	686.0
1	741.5
2	1115.5
3	1533.0
4	1877.0
5	2354.0
6	2787.0
7	3726.0
8	4917.5
9	5638.5
10	5965.0
11	6286.0
12	6945.5
13	8300.0
14	10709.5
15	13161.5
16	14571.0
17	14860.5
18	14168.0
19	12971.5
20	11966.0
21	11743.0
22	12828.5
23	15478.5
24	20161.5
25	26347.5
26	34341.5
27	43702.0
28	53320.0
29	63742.0
30	73546.0
31	83248.0
32	93909.5
33	104267.0
34	114795.0
35	124992.5
36	135178.5
37	144316.5
38	151628.5
39	158463.5
40	165921.5
41	171534.0
42	174956.0
43	178983.0
44	181790.0
45	183308.5
46	183202.0
47	182480.0
48	180854.0
49	176475.0
50	170349.0
51	163084.0
52	154822.5
53	145057.0
54	134912.5
55	123875.5
56	111266.5
57	99022.5
58	87144.0
59	75126.5
60	62571.0
61	49827.0
62	38387.5
63	29275.0
64	21788.0
65	15724.0
66	11309.0
67	8326.5
68	6284.5
69	4478.0
70	3101.5
71	2197.0
72	1531.0
73	985.0
74	601.0
75	394.0
76	249.5
77	144.0
78	87.0
79	57.0
80	42.5
81	29.5
82	23.0
83	19.0
84	13.0
85	8.5
86	5.0
87	3.5
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.00955437515196199
6	6.940224081328321E-5
7	0.0
8	0.0
9	2.3134080271094407E-5
10-11	1.1567040135547204E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.1644774240208443E-4
26-27	0.0
28-29	4.976194118786614E-4
30-31	0.0022356230901639535
32-33	1.509005746293882E-4
34-35	3.4915408693587606E-5
36-37	2.687426373280719E-4
38-39	6.571380861862417E-4
40-41	4.4808055262010387E-4
42-43	1.3053044130087587E-4
44-45	0.0
46-47	0.0012899273192260292
48-49	4.863176356890033E-5
50-51	0.0017282264609121138
52-53	0.0013148505488026442
54-55	0.002570068174439996
56-57	0.0023133504562283945
58-59	0.0033983062398443282
60-61	0.0025157348058602496
62-63	0.0027114886147466995
64-65	0.003880366780331177
66-67	0.0014234500947928796
68-69	6.413699251123648E-5
70-71	5.439071648890831E-4
72-73	0.006234884978789627
74-75	0.005762249328968265
76-77	0.004537941717391362
78-79	0.0024049917417420445
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020725116853707324
90-91	1.709383933756814E-4
92-93	0.0
94-95	8.728676933336476E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	21.0
20-21	93.0
22-23	207.0
24-25	274.0
26-27	940.0
28-29	2616.0
30-31	8621.0
32-33	10543.0
34-35	14751.0
36-37	23192.0
38-39	14528.0
40-41	26483.0
42-43	29251.0
44-45	34534.0
46-47	35505.0
48-49	33710.0
50-51	17735.0
52-53	2903.0
54-55	2693.0
56-57	2533.0
58-59	18385.0
60-61	33444.0
62-63	32633.0
64-65	34038.0
66-67	35804.0
68-69	36916.0
70-71	36632.0
72-73	33415.0
74-75	34283.0
76-77	35409.0
78-79	36349.0
80-81	36042.0
82-83	34508.0
84-85	33569.0
86-87	34648.0
88-89	36249.0
90-91	36316.0
92-93	36178.0
94-95	42017.0
96-97	143491.0
98-99	324925.0
100-101	2936243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.9973541465662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.53265577426416	80.5463152614366
2	6.197161655869172	10.906676578700802
3	1.324858136715897	3.4975203195164575
4	0.4535775895794176	1.5965451113266342
5	0.19845307987071967	0.8731672975430254
6	0.09990394442140392	0.5274769666733489
7	0.052103427726837445	0.3209474647349972
8	0.03765733450305944	0.26509966403851426
9	0.024616709051223803	0.19495847378731707
>10	0.07693282782172771	1.0028382760890446
>50	0.0011495558544523188	0.07149276299575673
>100	8.195002675267467E-4	0.13070011132733658
>500	1.1046405448765671E-4	0.06626171183018904
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3134080271094407E-5
2	0.0	2.3134080271094407E-5	0.0	0.0	2.3134080271094407E-5
3	0.0	2.3134080271094407E-5	0.0	0.0	2.3134080271094407E-5
4	0.0	4.6268160542188814E-5	0.0	2.3134080271094407E-5	4.6268160542188814E-5
5	0.0	4.6268160542188814E-5	0.0	4.6268160542188814E-5	4.6268160542188814E-5
6	0.0	4.6268160542188814E-5	0.0	4.6268160542188814E-5	4.6268160542188814E-5
7	0.0	4.6268160542188814E-5	0.0	4.6268160542188814E-5	6.940224081328322E-5
8	0.0	4.6268160542188814E-5	0.0	4.6268160542188814E-5	6.940224081328322E-5
9	0.0	9.253632108437763E-5	0.0	4.6268160542188814E-5	6.940224081328322E-5
10-11	0.0	9.253632108437763E-5	0.0	4.6268160542188814E-5	6.940224081328322E-5
12-13	0.0	9.253632108437763E-5	0.0	4.6268160542188814E-5	9.253632108437763E-5
14-15	0.0	1.3880448162656645E-4	0.0	4.6268160542188814E-5	1.7350560203320805E-4
16-17	1.1567040135547204E-5	1.3880448162656645E-4	0.0	5.7835200677736016E-5	2.0820672243984965E-4
18-19	2.3134080271094407E-5	1.7350560203320805E-4	0.0	8.096928094883043E-5	3.007430435242273E-4
20-21	2.3134080271094407E-5	2.1977376257539686E-4	0.0	1.0410336121992482E-4	3.701452843375105E-4
22-23	2.3134080271094407E-5	2.776089632531329E-4	0.0	1.1567040135547203E-4	4.048464047441521E-4
24-25	2.3134080271094407E-5	3.1231008365977447E-4	0.0	1.3880448162656645E-4	4.8581568569298255E-4
26-27	2.3134080271094407E-5	3.238771237953217E-4	0.0	1.8507264216875523E-4	5.08949765964077E-4
28-29	2.3134080271094407E-5	3.470112040664161E-4	0.0	4.279804850152465E-4	5.08949765964077E-4
30-31	4.6268160542188814E-5	4.048464047441521E-4	0.0	0.0014458800169434003	5.08949765964077E-4
32-33	4.6268160542188814E-5	4.164134448796993E-4	0.0	0.0029611622747000837	5.08949765964077E-4
34-35	4.6268160542188814E-5	4.8581568569298255E-4	0.0	0.0057603859875025075	5.08949765964077E-4
36-37	4.6268160542188814E-5	5.667849666418129E-4	0.0	0.011231595971616335	5.08949765964077E-4
38-39	4.6268160542188814E-5	5.899190469129073E-4	0.0	0.1719093504945025	5.552179265062658E-4
40-41	4.6268160542188814E-5	6.246201673195489E-4	0.0	0.3378154071586561	5.552179265062658E-4
42-43	6.940224081328322E-5	6.361872074550962E-4	0.0	0.3528294252545964	5.667849666418129E-4
44-45	6.940224081328322E-5	6.940224081328322E-4	0.0	0.37190347443811367	6.014860870484545E-4
46-47	6.940224081328322E-5	7.40290568675021E-4	0.0	0.3930711578861651	6.477542475906434E-4
48-49	6.940224081328322E-5	7.40290568675021E-4	0.0	0.41329034404310155	6.477542475906434E-4
50-51	9.253632108437763E-5	7.865587292172098E-4	0.0	0.4339953458857311	6.477542475906434E-4
52-53	9.253632108437763E-5	8.328268897593986E-4	0.0	0.941256323990018	7.171564884039266E-4
54-55	9.253632108437763E-5	8.675280101660402E-4	0.0	1.6073558972356392	7.981257693527571E-4
56-57	9.253632108437763E-5	9.022291305726818E-4	0.0	2.325692223733392	8.443939298949458E-4
58-59	9.253632108437763E-5	9.484972911148707E-4	0.0	3.0658902560873282	8.790950503015874E-4
60-61	9.253632108437763E-5	9.831984115215124E-4	0.0	3.2599967566019457	8.790950503015874E-4
62-63	9.253632108437763E-5	9.947654516570595E-4	0.0	3.2664511649975814	8.790950503015874E-4
64-65	9.253632108437763E-5	0.0010873017727414372	0.0	3.2722578191456257	8.906620904371346E-4
66-67	9.253632108437763E-5	0.0011220028931480787	0.0	3.277682760969198	9.022291305726818E-4
68-69	9.253632108437763E-5	0.0011335699332836258	0.0	3.2825409178261276	9.369302509793235E-4
70-71	1.1567040135547203E-4	0.0011451369734191731	0.0	3.28671661931506	9.716313713859651E-4
72-73	1.2723744149101924E-4	0.0011567040135547202	0.0	3.2904874743992485	0.001017899531928154
74-75	1.3880448162656645E-4	0.0011682710536902673	0.0	3.2933676673929995	0.001017899531928154
76-77	1.5037152176211366E-4	0.0012492403346390979	0.0	3.29612062294526	0.001017899531928154
78-79	1.6193856189766084E-4	0.0012723744149101923	0.0	3.297774709684643	0.001017899531928154
80-81	1.6193856189766084E-4	0.0012955084951812867	0.0	3.2983299276111495	0.0010410336121992483
82-83	1.7350560203320805E-4	0.0012955084951812867	0.0	3.2987694751363	0.0010988688128769843
84-85	1.8507264216875526E-4	0.0013186425754523811	0.0	3.2989429807383335	0.0011104358530125316
86-87	1.8507264216875526E-4	0.0013417766557234756	0.0	3.299035517059418	0.0011220028931480787
88	1.8507264216875526E-4	0.0014574470570789476	0.0	3.299035517059418	0.0011335699332836258
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5180	0.0	48.94218	1
ATCTCGT	4145	0.0	45.868423	94
GTATCAA	9705	0.0	36.58963	1
TATGCCG	1965	0.0	35.60241	86-87
TATCTCG	4905	0.0	33.018963	94
GCCGTCT	2205	0.0	32.842014	90-91
CCGTCTT	2200	0.0	32.790535	90-91
ATGCCGT	2220	0.0	32.240208	88-89
TGCCGTC	2210	0.0	32.13887	88-89
ATCAACG	11665	0.0	32.04193	3
TCAACGC	11700	0.0	31.908974	4
CAACGCA	12005	0.0	31.098291	5
TCTCGTA	2240	0.0	31.040293	82-83
TATCAAC	12295	0.0	30.611937	2
TCGTATG	2305	0.0	30.487795	84-85
CTCGTAT	2275	0.0	30.330334	82-83
CGTATGC	2310	0.0	30.306133	84-85
AACGCAG	12440	0.0	29.973183	6
ACGCAGA	14405	0.0	25.88451	7
CGCAGAG	14685	0.0	25.361412	8
>>END_MODULE