FastQCFastQC Report
Fri 27 May 2016
SRR2138857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10137308
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128750.049.9267851
GTATCAA231550.038.8171731
ATCAACG274300.032.0616153
TCAACGC276450.031.8589534
CAACGCA281700.031.2652035
TATCAAC289250.030.5978722
AACGCAG294400.029.9871756
ACGCAGA332650.026.500287
CGCAGAG338700.026.0015148
GTGGTAT64050.022.4366281
TGGTATC60050.021.9249552
GCAGAGT403450.021.8285129
GAGTACT232400.020.61342212-13
GTACATG297100.020.5499131
TACATGG290350.020.197812
ACATGGG292450.019.4642853
CAGAGTA388700.019.37508410-11
AGAGTAC358900.018.82011210-11
GTACTTT264450.018.0748214-15
CATGGGG214450.017.5153374
Produced by FastQC (version 0.11.5)