##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10137308
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.716531844548868	32.0	30.0	33.0	18.0	34.0
2	31.552576482829565	33.0	32.0	33.0	27.0	34.0
3	32.24711856441572	33.0	33.0	33.0	30.0	34.0
4	32.307880553693344	33.0	33.0	33.0	31.0	34.0
5	32.96952731435209	33.0	33.0	34.0	32.0	34.0
6	36.90283278361474	38.0	37.0	38.0	35.0	38.0
7	37.279012238752145	38.0	38.0	38.0	36.0	38.0
8	37.2381259403384	38.0	38.0	38.0	36.0	38.0
9	37.382262332366736	38.0	38.0	38.0	37.0	38.0
10-11	37.44647513915923	38.0	38.0	38.0	37.0	38.0
12-13	37.46293927342447	38.0	38.0	38.0	37.0	38.0
14-15	37.457862975062014	38.0	38.0	38.0	37.0	38.0
16-17	37.45728417248445	38.0	38.0	38.0	37.0	38.0
18-19	37.45809553187098	38.0	38.0	38.0	37.0	38.0
20-21	37.46068079926286	38.0	38.0	38.0	37.0	38.0
22-23	37.46789294523403	38.0	38.0	38.0	37.0	38.0
24-25	37.430345572568214	38.0	38.0	38.0	37.0	38.0
26-27	37.43730323434258	38.0	38.0	38.0	37.0	38.0
28-29	37.43123691630073	38.0	38.0	38.0	37.0	38.0
30-31	37.4253231639742	38.0	38.0	38.0	37.0	38.0
32-33	37.42549129657663	38.0	38.0	38.0	37.0	38.0
34-35	37.41087022953715	38.0	38.0	38.0	37.0	38.0
36-37	37.387009975815914	38.0	38.0	38.0	37.0	38.0
38-39	37.36428229228185	38.0	38.0	38.0	37.0	38.0
40-41	37.351148902421514	38.0	38.0	38.0	37.0	38.0
42-43	37.33497033208679	38.0	38.0	38.0	37.0	38.0
44-45	37.302738356111405	38.0	38.0	38.0	37.0	38.0
46-47	37.26766828540392	38.0	38.0	38.0	37.0	38.0
48-49	37.22204721650433	38.0	38.0	38.0	37.0	38.0
50-51	37.19811062937667	38.0	38.0	38.0	37.0	38.0
52-53	37.18435975515146	38.0	38.0	38.0	37.0	38.0
54-55	37.15962833812455	38.0	38.0	38.0	37.0	38.0
56-57	37.12251483493347	38.0	38.0	38.0	36.0	38.0
58-59	37.12168081431024	38.0	38.0	38.0	36.0	38.0
60-61	37.13208707820188	38.0	38.0	38.0	36.0	38.0
62-63	37.13772113644469	38.0	38.0	38.0	36.0	38.0
64-65	37.14186154411049	38.0	38.0	38.0	36.0	38.0
66-67	37.14750351583171	38.0	38.0	38.0	36.0	38.0
68-69	37.14432349019655	38.0	38.0	38.0	36.0	38.0
70-71	37.107588621644	38.0	38.0	38.0	36.0	38.0
72-73	37.08290600440084	38.0	38.0	38.0	36.0	38.0
74-75	37.06373490861706	38.0	38.0	38.0	36.0	38.0
76-77	37.064608898812835	38.0	38.0	38.0	36.0	38.0
78-79	37.06432792424627	38.0	38.0	38.0	36.0	38.0
80-81	37.05169252554215	38.0	38.0	38.0	36.0	38.0
82-83	37.050878193581006	38.0	38.0	38.0	36.0	38.0
84-85	37.03926503557513	38.0	38.0	38.0	36.0	38.0
86-87	37.007281081722155	38.0	38.0	38.0	36.0	38.0
88-89	36.96607728249915	38.0	38.0	38.0	36.0	38.0
90-91	36.96850913429922	38.0	38.0	38.0	36.0	38.0
92-93	36.9441894914396	38.0	38.0	38.0	35.5	38.0
94-95	36.928338711715256	38.0	38.0	38.0	35.5	38.0
96-97	36.91832724380904	38.0	38.0	38.0	35.0	38.0
98-99	36.960131608144536	38.0	38.0	38.0	35.0	38.0
100	35.27236538944047	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	32.0
20	143.0
21	394.0
22	1050.0
23	2230.0
24	4617.0
25	8509.0
26	14563.0
27	23058.0
28	35264.0
29	52084.0
30	73070.0
31	97032.0
32	126573.0
33	168476.0
34	244485.0
35	428197.0
36	1237142.0
37	7620382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11185967714506	19.220033563151084	14.135172769733344	23.532933989970513
2	14.270928731769816	20.268980680077988	43.18303241846849	22.27705816968371
3	18.21246824107544	26.041331682928053	30.108348291282066	25.637851784714442
4	12.261105216493373	18.06157019200758	38.9098466772441	30.767477914254947
5	13.42039630561459	37.48415514477233	35.602246558525565	13.493201991087519
6	30.006774976857752	37.71860841896426	18.88896692848513	13.385649675692868
7	26.64249719945374	32.66830799656083	22.927980485549025	17.761214318436412
8	24.123110395777655	37.10310469012089	21.09741560579988	17.676369308301574
9	25.46479059971253	17.32863570176971	21.30905180241656	35.8975218961012
10-11	24.133248603401277	26.88548842409528	28.860810864266025	20.120452108237423
12-13	25.038767688621082	24.296647591254008	29.021457175810383	21.643127544314524
14-15	22.11301067305048	25.68994648283351	26.790682496773304	25.406360347342705
16-17	21.67952774050073	28.8549780671555	27.459686536109977	22.005807656233785
18-19	21.656133955878623	27.940243109906497	29.272189421491383	21.131433512723497
20-21	22.45647270321732	27.096480863259902	29.17927945008889	21.26776698343389
22-23	22.17341790658127	27.038383900961776	29.06373614535634	21.72446204710061
24-25	21.96982372757083	27.115522695689386	29.167387676813554	21.747265899926223
26-27	21.924126047426657	27.290468912572603	29.099217882948686	21.68618715705205
28-29	21.812325056313103	27.383327531691325	29.134059777211025	21.670287634784547
30-31	22.102676274883784	27.206637009581957	29.056219628057384	21.634467087476875
32-33	21.8976579742486	27.37626500412128	28.99377495573528	21.732302065894842
34-35	22.058296049764667	27.33901348160946	29.032591531068068	21.570098937557805
36-37	21.973129352160683	27.30335679741408	29.006827852852666	21.716685997572565
38-39	21.802083467832787	27.36209814941766	29.140984152636435	21.694834230113123
40-41	22.09740874427052	27.191802457178827	29.004571501346682	21.706217297203967
42-43	21.998466793014373	27.275589221045475	28.8513976677004	21.874546318239748
44-45	22.013279750949604	27.44913222317944	28.744490224967695	21.793097800903265
46-47	22.073334805383098	27.340466606880064	28.50708660381261	22.07911198392423
48-49	22.04824667645089	27.399811655835364	28.532612426828813	22.019329240884932
50-51	21.96227746138306	27.576859795124083	28.477649315989094	21.983213427503763
52-53	22.15397802041357	27.50664653722652	28.36196023680288	21.977415205557023
54-55	22.1021030686143	27.439575049791245	28.37361875089726	22.084703130697196
56-57	22.163298382697914	27.528273977830025	28.27501233001339	22.03341530945867
58-59	22.05633386699115	27.601845163142325	28.393707168935883	21.948113800930642
60-61	22.12430469671765	27.616524313578832	28.32473780827786	21.93443318142565
62-63	22.07532469588031	27.709632251027376	28.249945625907884	21.96509742718443
64-65	22.01692556805573	27.6888788937394	28.269964317829764	22.024231220375114
66-67	22.123980902730775	27.68906531764944	28.12527046105871	22.061683318561073
68-69	22.0858504190257	27.649964044165387	28.19030920002494	22.073876336783965
70-71	22.10717585968132	27.395506658481576	28.234849090520264	22.26246839131684
72-73	22.16615200892205	27.394978105560586	28.21409161348968	22.224778272027688
74-75	22.062558530698272	27.53381262353285	28.168814613864214	22.234814231904668
76-77	22.159516075232137	27.482847498332053	28.059932553307494	22.29770387312832
78-79	22.193728287295677	27.533035159871194	27.991635811681174	22.281600741151955
80-81	22.145789144526766	27.56116432019869	27.98907584158257	22.303970693691976
82-83	22.265271546509847	27.516056572954916	28.050820428078264	22.167851452456965
84-85	22.368378217842398	27.5370424092885	27.917231329742958	22.17734804312615
86-87	22.13372866616821	27.580573494104073	28.0066216954518	22.279076144275923
88-89	22.310286673774694	27.55574525546604	27.930265016277446	22.203703054481817
90-91	22.35136589847695	27.569061190677147	27.915057279373954	22.164515631471954
92-93	22.3590245828522	27.60838928614274	27.915089638585577	22.11749649241948
94-95	22.24265959935863	27.718713694686432	27.895575369262467	22.143051336692476
96-97	22.34435489501713	27.77875553160219	27.981948805066214	21.894940768314466
98-99	23.373452914338845	29.71162365496805	28.168353429576676	18.746570001116424
100	22.318606052307672	30.590753708659392	22.108822413239572	24.981817825793364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1771.0
1	1553.5
2	2210.0
3	3450.5
4	4314.5
5	5679.5
6	7099.5
7	9328.0
8	12642.0
9	14828.0
10	15037.5
11	14879.5
12	16083.0
13	19093.0
14	24556.5
15	30402.0
16	34459.5
17	36849.5
18	36533.0
19	33988.0
20	31095.5
21	29509.0
22	31254.0
23	37158.5
24	47302.0
25	61570.0
26	80237.0
27	101221.0
28	121428.0
29	143467.5
30	164980.0
31	185810.5
32	209151.5
33	232966.5
34	257237.5
35	280768.0
36	304888.5
37	327535.0
38	344638.5
39	359194.0
40	376105.5
41	390282.0
42	398972.5
43	412529.0
44	423901.0
45	431300.0
46	435756.0
47	434183.0
48	430301.5
49	421645.0
50	411661.0
51	396301.0
52	375754.5
53	355055.5
54	333791.5
55	309296.5
56	283109.0
57	256861.5
58	228709.0
59	198379.0
60	164633.0
61	132041.0
62	103641.0
63	80300.0
64	60510.0
65	44143.0
66	32207.0
67	24087.0
68	18255.5
69	13239.5
70	9436.5
71	6682.5
72	4612.0
73	2980.0
74	1888.0
75	1190.5
76	736.5
77	442.0
78	297.5
79	208.0
80	134.5
81	84.0
82	61.0
83	47.0
84	35.0
85	22.0
86	11.5
87	5.5
88	4.5
89	5.0
90	4.0
91	2.0
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.007270174685429307
6	3.9458207247920256E-5
7	0.0
8	0.0
9	9.864551811980064E-6
10-11	9.864551811980064E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.919318504914317E-4
26-27	0.0
28-29	7.106015133246173E-4
30-31	0.002355981144643314
32-33	1.0391640133345525E-4
34-35	5.456431485871613E-5
36-37	2.4392774242982694E-4
38-39	4.8044224708844495E-4
40-41	6.191611343193063E-4
42-43	1.468489903220439E-4
44-45	0.0
46-47	0.0014699722195809551
48-49	5.699545062313126E-5
50-51	0.0017803085655865936
52-53	0.0014457070753535121
54-55	0.0024932054857084536
56-57	0.0021915988497465426
58-59	0.00356260726107721
60-61	0.0025616671264669607
62-63	0.0028270999690383435
64-65	0.0041257629360821405
66-67	0.0016310616269451382
68-69	1.1755747146866172E-4
70-71	6.667053506437915E-4
72-73	0.006564934704566245
74-75	0.005901616939504284
76-77	0.004722658404141784
78-79	0.0027106298432383632
80-81	2.363563866686489E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020838226230570807
90-91	3.0947676021406384E-4
92-93	1.2503070285196908E-5
94-95	1.0106732144815322E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	62.0
20-21	280.0
22-23	535.0
24-25	738.0
26-27	2209.0
28-29	6104.0
30-31	17975.0
32-33	22765.0
34-35	31118.0
36-37	50219.0
38-39	58264.0
40-41	57695.0
42-43	64719.0
44-45	76266.0
46-47	79459.0
48-49	74591.0
50-51	67629.0
52-53	63961.0
54-55	69144.0
56-57	70344.0
58-59	72834.0
60-61	71371.0
62-63	71429.0
64-65	75180.0
66-67	80072.0
68-69	81919.0
70-71	85053.0
72-73	74805.0
74-75	73582.0
76-77	76929.0
78-79	78376.0
80-81	79030.0
82-83	75190.0
84-85	74088.0
86-87	75815.0
88-89	79061.0
90-91	80439.0
92-93	80789.0
94-95	94766.0
96-97	310367.0
98-99	688895.0
100-101	6843241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.37729237499892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97590430515511	72.47216790900688
2	8.067162642947888	13.291020313495745
3	2.154538091294946	5.3245504283902765
4	0.8236413834914256	2.7139738824008703
5	0.3817876348429357	1.5725315810307918
6	0.2046554186614177	1.0115375555519648
7	0.12242336254958841	0.7059433591194555
8	0.07160251334887963	0.47187369415403346
9	0.047428478998318	0.3516326713211344
>10	0.14789605645649764	1.8136857084395324
>50	0.0021418196756264533	0.12154029043790703
>100	7.764506367492357E-4	0.10628494318324205
>500	0.0	0.0
>1k	4.184194060400886E-5	0.04325766346822253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.864551811980064E-6	0.0	0.0	0.0
3	0.0	9.864551811980064E-6	0.0	0.0	0.0
4	0.0	2.959365543594019E-5	0.0	9.864551811980064E-6	0.0
5	0.0	2.959365543594019E-5	0.0	9.864551811980064E-6	0.0
6	0.0	2.959365543594019E-5	0.0	2.959365543594019E-5	9.864551811980064E-6
7	0.0	2.959365543594019E-5	0.0	6.905186268386045E-5	9.864551811980064E-6
8	0.0	5.918731087188038E-5	0.0	7.891641449584051E-5	9.864551811980064E-6
9	0.0	9.864551811980065E-5	0.0	1.5783282899168102E-4	9.864551811980064E-6
10-11	0.0	9.864551811980065E-5	0.0	1.972910362396013E-4	9.864551811980064E-6
12-13	0.0	9.864551811980065E-5	0.0	2.170201398635614E-4	1.4796827717970096E-5
14-15	4.932275905990032E-6	1.7756193261564114E-4	0.0	2.5154607120549165E-4	1.9729103623960128E-5
16-17	9.864551811980064E-6	1.8742648442762123E-4	0.0	2.910042784534119E-4	1.9729103623960128E-5
18-19	9.864551811980064E-6	1.8742648442762123E-4	0.0	3.255302097953421E-4	1.9729103623960128E-5
20-21	9.864551811980064E-6	1.8742648442762123E-4	0.0	3.7485296885524246E-4	2.959365543594019E-5
22-23	9.864551811980064E-6	2.170201398635614E-4	0.0	4.1924345200915273E-4	4.932275905990032E-5
24-25	9.864551811980064E-6	2.5154607120549165E-4	0.0	4.488371074450929E-4	5.918731087188038E-5
26-27	9.864551811980064E-6	2.762074507354418E-4	0.0	5.030921424109833E-4	5.918731087188038E-5
28-29	1.9729103623960128E-5	2.762074507354418E-4	0.0	7.497059377104848E-4	5.918731087188038E-5
30-31	1.9729103623960128E-5	3.0086883026539195E-4	0.0	0.0017016351875665612	5.918731087188038E-5
32-33	1.9729103623960128E-5	3.05801106171382E-4	0.0	0.0037633265162703945	5.918731087188038E-5
34-35	1.9729103623960128E-5	3.551238652312823E-4	0.0	0.006490875092282883	5.918731087188038E-5
36-37	1.9729103623960128E-5	4.093789001971727E-4	0.0	0.010821413337742131	5.918731087188038E-5
38-39	1.9729103623960128E-5	4.1924345200915273E-4	0.0	0.020365367215832843	6.905186268386045E-5
40-41	1.9729103623960128E-5	4.6856621106905304E-4	0.0	0.03098948951733537	6.905186268386045E-5
42-43	1.9729103623960128E-5	5.080244183169733E-4	0.0	0.038363241996790476	6.905186268386045E-5
44-45	1.9729103623960128E-5	5.524149014708836E-4	0.0	0.04686648565871729	6.905186268386045E-5
46-47	1.9729103623960128E-5	5.820085569068238E-4	0.0	0.05642030408861998	6.905186268386045E-5
48-49	1.9729103623960128E-5	5.918731087188038E-4	0.0	0.06561899865329138	6.905186268386045E-5
50-51	1.9729103623960128E-5	6.461281436846942E-4	0.0	0.0746697249407831	6.905186268386045E-5
52-53	1.9729103623960128E-5	6.510604195906842E-4	0.0	0.09603634416553192	7.891641449584051E-5
54-55	1.9729103623960128E-5	6.510604195906842E-4	0.0	0.12213301598412518	9.37132422138106E-5
56-57	1.9729103623960128E-5	6.609249714026643E-4	0.0	0.16297226048572264	9.864551811980065E-5
58-59	1.9729103623960128E-5	6.609249714026643E-4	0.0	0.1936263552414507	1.085100699317807E-4
60-61	1.9729103623960128E-5	6.855863509326144E-4	0.0	0.20675114142729017	1.085100699317807E-4
62-63	1.9729103623960128E-5	7.003831786505845E-4	0.0	0.21700534303584346	1.085100699317807E-4
64-65	1.9729103623960128E-5	8.483514558302855E-4	0.0	0.22175019245740585	1.085100699317807E-4
66-67	1.9729103623960128E-5	8.483514558302855E-4	0.0	0.22674165567426777	1.1344234583777074E-4
68-69	1.9729103623960128E-5	8.730128353602357E-4	0.0	0.23204878454911304	1.1837462174376077E-4
70-71	1.9729103623960128E-5	9.026064907961759E-4	0.0	0.2367196498320856	1.1837462174376077E-4
72-73	2.4661379529950158E-5	9.17403318514146E-4	0.0	0.24030541441574035	1.381037253677209E-4
74-75	2.959365543594019E-5	9.32200146232116E-4	0.0	0.24307735347490675	1.381037253677209E-4
76-77	3.9458207247920256E-5	9.716583534800363E-4	0.0	0.24601205763897083	1.4796827717970096E-4
78-79	3.9458207247920256E-5	9.963197330099865E-4	0.0	0.24766930234338347	1.4796827717970096E-4
80-81	3.9458207247920256E-5	9.963197330099865E-4	0.0	0.2482167849689484	1.5783282899168102E-4
82-83	3.9458207247920256E-5	0.0010012520089159767	0.0	0.24856697655827364	1.7756193261564114E-4
84-85	3.9458207247920256E-5	0.0010160488366339467	0.0	0.2488481162849151	1.7756193261564114E-4
86-87	4.932275905990032E-5	0.0010160488366339467	0.0	0.24893689725122292	1.7756193261564114E-4
88	4.932275905990032E-5	0.0010357779402579068	0.0	0.2489418295271289	1.7756193261564114E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12875	0.0	49.926785	1
GTATCAA	23155	0.0	38.817173	1
ATCAACG	27430	0.0	32.061615	3
TCAACGC	27645	0.0	31.858953	4
CAACGCA	28170	0.0	31.265203	5
TATCAAC	28925	0.0	30.597872	2
AACGCAG	29440	0.0	29.987175	6
ACGCAGA	33265	0.0	26.50028	7
CGCAGAG	33870	0.0	26.001514	8
GTGGTAT	6405	0.0	22.436628	1
TGGTATC	6005	0.0	21.924955	2
GCAGAGT	40345	0.0	21.828512	9
GAGTACT	23240	0.0	20.613422	12-13
GTACATG	29710	0.0	20.549913	1
TACATGG	29035	0.0	20.19781	2
ACATGGG	29245	0.0	19.464285	3
CAGAGTA	38870	0.0	19.375084	10-11
AGAGTAC	35890	0.0	18.820112	10-11
GTACTTT	26445	0.0	18.07482	14-15
CATGGGG	21445	0.0	17.515337	4
>>END_MODULE