FastQCFastQC Report
Fri 27 May 2016
SRR2138856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5792095
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.047.8069271
GTATCAA127000.039.5485531
TCAACGC147050.033.2187844
ATCAACG147550.033.0770233
CAACGCA150650.032.4249765
TATCAAC158100.031.2784882
AACGCAG157200.031.235926
ACGCAGA179800.027.3336727
CGCAGAG181600.027.0153058
GCAGAGT217750.022.5501129
GTACATG155250.021.2258741
TACATGG154700.020.744442
GAGTACT127450.020.64920612-13
ACATGGG156550.019.9489783
CAGAGTA210400.019.21279510-11
AGAGTAC192950.019.13101610-11
GTGGTAT36950.019.1191
CATGGGG123700.018.5954744
GTACTTT142900.018.3715314-15
TGGTATC35950.017.6138442
Produced by FastQC (version 0.11.5)