##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5792095
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.327845624079025	32.0	28.0	33.0	18.0	34.0
2	31.426851769523807	33.0	32.0	33.0	27.0	34.0
3	32.170791397585845	33.0	33.0	33.0	29.0	34.0
4	32.23250240198063	33.0	33.0	33.0	31.0	34.0
5	32.94915397623831	33.0	33.0	34.0	32.0	34.0
6	36.889466419318055	38.0	37.0	38.0	35.0	38.0
7	37.2586684092716	38.0	38.0	38.0	36.0	38.0
8	37.218050290956896	38.0	38.0	38.0	36.0	38.0
9	37.360355277321936	38.0	38.0	38.0	37.0	38.0
10-11	37.42963651321327	38.0	38.0	38.0	37.0	38.0
12-13	37.45115463403138	38.0	38.0	38.0	37.0	38.0
14-15	37.44564773195191	38.0	38.0	38.0	37.0	38.0
16-17	37.44515361022221	38.0	38.0	38.0	37.0	38.0
18-19	37.44733796665973	38.0	38.0	38.0	37.0	38.0
20-21	37.449403433933114	38.0	38.0	38.0	37.0	38.0
22-23	37.45742510677614	38.0	38.0	38.0	37.0	38.0
24-25	37.41849201347216	38.0	38.0	38.0	37.0	38.0
26-27	37.426866499214526	38.0	38.0	38.0	37.0	38.0
28-29	37.42393155161643	38.0	38.0	38.0	37.0	38.0
30-31	37.41805561565086	38.0	38.0	38.0	37.0	38.0
32-33	37.416441726218665	38.0	38.0	38.0	37.0	38.0
34-35	37.4043111923427	38.0	38.0	38.0	37.0	38.0
36-37	37.383421514654856	38.0	38.0	38.0	37.0	38.0
38-39	37.36345722650677	38.0	38.0	38.0	37.0	38.0
40-41	37.34503144443529	38.0	38.0	38.0	37.0	38.0
42-43	37.32746907857033	38.0	38.0	38.0	37.0	38.0
44-45	37.298919129310114	38.0	38.0	38.0	37.0	38.0
46-47	37.268762562599036	38.0	38.0	38.0	37.0	38.0
48-49	37.22508697316783	38.0	38.0	38.0	37.0	38.0
50-51	37.202679267237926	38.0	38.0	38.0	37.0	38.0
52-53	37.18608790295626	38.0	38.0	38.0	37.0	38.0
54-55	37.15946413265229	38.0	38.0	38.0	36.5	38.0
56-57	37.1215397270292	38.0	38.0	38.0	36.0	38.0
58-59	37.12218900016525	38.0	38.0	38.0	36.0	38.0
60-61	37.132901768990514	38.0	38.0	38.0	36.0	38.0
62-63	37.137062566003976	38.0	38.0	38.0	36.0	38.0
64-65	37.139699686165045	38.0	38.0	38.0	36.0	38.0
66-67	37.1422648242979	38.0	38.0	38.0	36.0	38.0
68-69	37.13851539162137	38.0	38.0	38.0	36.0	38.0
70-71	37.1008161234011	38.0	38.0	38.0	36.0	38.0
72-73	37.074563893434615	38.0	38.0	38.0	36.0	38.0
74-75	37.054157989669434	38.0	38.0	38.0	36.0	38.0
76-77	37.05175304805009	38.0	38.0	38.0	36.0	38.0
78-79	37.05255964294808	38.0	38.0	38.0	36.0	38.0
80-81	37.03993637809197	38.0	38.0	38.0	36.0	38.0
82-83	37.03685891997077	38.0	38.0	38.0	36.0	38.0
84-85	37.02558276547614	38.0	38.0	38.0	36.0	38.0
86-87	36.99114882659096	38.0	38.0	38.0	36.0	38.0
88-89	36.946579084039236	38.0	38.0	38.0	36.0	38.0
90-91	36.94832064935929	38.0	38.0	38.0	36.0	38.0
92-93	36.921918028547076	38.0	38.0	38.0	35.0	38.0
94-95	36.90660416144021	38.0	38.0	38.0	35.0	38.0
96-97	36.8958611225915	38.0	38.0	38.0	35.0	38.0
98-99	36.94069600147063	38.0	38.0	38.0	35.0	38.0
100	35.217998624312855	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	13.0
20	91.0
21	255.0
22	600.0
23	1258.0
24	2596.0
25	4968.0
26	8248.0
27	13079.0
28	20009.0
29	29435.0
30	40902.0
31	54885.0
32	71998.0
33	96388.0
34	142023.0
35	252913.0
36	736856.0
37	4315574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34625036364217	19.14542147530384	13.973320534279912	23.535007626774078
2	14.334519720412045	20.3748729950044	42.96295209246395	22.327655192119604
3	18.170765500220558	26.04042233423312	30.106256889778223	25.68255527576809
4	12.34879607465002	17.971183138398107	38.842266917238064	30.837753869713808
5	13.50503400810849	37.45220536881441	35.56333219204632	13.479428431030783
6	30.251867546302787	37.6054454936144	18.823647828798297	13.319039131284516
7	26.704189071484496	32.567059069300484	23.086085431955105	17.64266642725991
8	24.148468584936637	37.16094731887984	21.109981985789595	17.580602110393926
9	25.536666784643554	17.121784086759625	21.390170568680244	35.95137855991658
10-11	24.221412843092672	26.74995433430466	28.961089029287162	20.067543793315508
12-13	24.997336887602845	24.28957052672651	29.127267422236685	21.585825163433956
14-15	22.088510288591607	25.65771970245654	26.7906603741824	25.46310963476946
16-17	21.724479657187942	28.79645447804292	27.46295597706875	22.01610988770039
18-19	21.761158958891386	28.003891510757335	29.135839450147138	21.099110080204138
20-21	22.469702311419184	27.119845456494847	29.128546729589956	21.281905502496016
22-23	22.251955594123185	26.999880521685142	28.971531701637094	21.77663218255458
24-25	22.028137812430124	27.113249687688583	29.006413715284225	21.852198784597068
26-27	21.99609846362975	27.25603223732061	28.95103514870873	21.796834150340906
28-29	21.894039931853744	27.309432051782313	28.98419898872284	21.812329027641102
30-31	22.18731976315582	27.125351137679164	28.927261423471695	21.76006767569332
32-33	21.990667309942115	27.27625588749019	28.85912224424497	21.87395455832273
34-35	22.1203550620685	27.280713374133054	28.87831559926924	21.72061596452921
36-37	22.06738030579706	27.238618677771566	28.878158729506076	21.815842286925303
38-39	21.93435975682909	27.38291081765877	28.930628756636263	21.75210066887588
40-41	22.21509777163431	27.21690246934436	28.794423175806443	21.773576583214886
42-43	22.06255660621494	27.291896661138086	28.68960449647922	21.955942236167754
44-45	22.15026618471196	27.36627166218357	28.57574320676849	21.907718946335976
46-47	22.144355027421877	27.384047315558025	28.421266398310717	22.05033125870938
48-49	22.109032623029893	27.48738779842656	28.368520539198116	22.03505903934543
50-51	22.137487074550897	27.605300025127555	28.277592234243226	21.97962066607832
52-53	22.270493065016762	27.47487576305493	28.191096294832708	22.0635348770956
54-55	22.218379392241328	27.43361472656337	28.223482830830882	22.12452305036442
56-57	22.19024023450421	27.504227840293392	28.199988259881497	22.1055436653209
58-59	22.21347321974462	27.526633531227883	28.224216281349147	22.03567696767835
60-61	22.255877881543785	27.46939702564905	28.27322912923171	22.001495963575454
62-63	22.237215906382982	27.492163822156034	28.22964719375043	22.04097307771056
64-65	22.19475445474443	27.532196554545536	28.21459810235466	22.05845088835537
66-67	22.2396127355116	27.538157036238154	28.096577490813324	22.125652737436923
68-69	22.180078796451745	27.50787979169823	28.159401590053907	22.152639821796118
70-71	22.236949395883148	27.371215924661165	28.135670214228313	22.256164465227375
72-73	22.275734790967206	27.395545222764206	28.066323030236834	22.262396956031758
74-75	22.159773046586633	27.494723429336542	28.079202019581555	22.266301504495267
76-77	22.30860515712589	27.4228225371339	27.97165936383233	22.29691294190788
78-79	22.239569786743907	27.486443936988813	27.926473696450838	22.347512579816442
80-81	22.24836825921412	27.545873176172442	27.90619117463215	22.29956738998129
82-83	22.300515498704765	27.511143181971565	27.93519148207175	22.25314983725192
84-85	22.377126485759042	27.512922421894608	27.88849700198136	22.221454090364986
86-87	22.25433064816714	27.54512995141254	27.91956738906926	22.28097201135106
88-89	22.30708723144306	27.565739688485415	27.86553261293297	22.261640467138555
90-91	22.386376267380573	27.564843528528826	27.80440398323917	22.244376220851432
92-93	22.412335835692758	27.538724349293	27.84829940941533	22.200640405598914
94-95	22.319495179944347	27.65304171227367	27.824458100902472	22.203005006879515
96-97	22.399928798402783	27.70573040135328	27.875124176187814	22.019216624056124
98-99	23.43826294900527	29.63572029127738	28.10409188662873	18.821924873088626
100	22.32718375082676	30.566893931267497	22.066001463129194	25.039920854776547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	781.0
1	776.0
2	1293.5
3	1976.5
4	2397.0
5	3115.5
6	3886.5
7	5159.5
8	6965.0
9	8048.5
10	8050.5
11	7934.5
12	8496.5
13	9999.5
14	12763.5
15	15618.0
16	17760.0
17	19155.0
18	19145.0
19	18199.0
20	17181.0
21	16611.5
22	17845.5
23	21140.0
24	26670.5
25	34918.0
26	45066.5
27	56185.5
28	68018.0
29	81024.0
30	94009.5
31	107243.0
32	121107.0
33	134300.5
34	146915.0
35	158234.5
36	170473.0
37	183860.0
38	195098.5
39	204105.0
40	213296.5
41	221195.0
42	226903.0
43	232227.0
44	235920.5
45	241109.5
46	245334.5
47	245851.0
48	245253.5
49	241486.5
50	235667.5
51	227260.0
52	216781.5
53	205889.5
54	193403.0
55	179443.5
56	164505.0
57	148586.0
58	132259.0
59	115273.0
60	96214.5
61	77715.0
62	61739.0
63	47866.5
64	35976.0
65	26488.0
66	19524.5
67	14865.0
68	11345.0
69	8165.5
70	5854.5
71	4190.0
72	2829.0
73	1852.0
74	1169.5
75	716.0
76	444.0
77	274.0
78	168.5
79	106.5
80	70.5
81	45.5
82	29.0
83	18.0
84	10.5
85	6.5
86	5.5
87	7.0
88	7.0
89	5.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006698785154594321
6	5.179473057676023E-5
7	0.0
8	1.7264910192253408E-5
9	0.0
10-11	1.7264910192253408E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.179982819723648E-4
26-27	8.634009102835797E-6
28-29	6.391045626883847E-4
30-31	0.0023339478243370594
32-33	1.3856210123884045E-4
34-35	2.6041628237180553E-5
36-37	2.3520382807297067E-4
38-39	4.466041403356309E-4
40-41	5.460744119747425E-4
42-43	1.5061006822990466E-4
44-45	0.0
46-47	0.0014383389055481496
48-49	9.965812732412946E-5
50-51	0.0018710689298142879
52-53	0.0016357884036019325
54-55	0.00251658936635518
56-57	0.002403871238966417
58-59	0.0036797025448616284
60-61	0.0026770352751897667
62-63	0.002697455917803657
64-65	0.0039861680927703305
66-67	0.0016754269869256651
68-69	1.3675511564256783E-4
70-71	6.306444526099763E-4
72-73	0.006102034969033166
74-75	0.005490959405878192
76-77	0.0043048135394267864
78-79	0.0025081006041973576
80-81	1.0289213323790431E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.002165100541723087
90-91	2.584945621360612E-4
92-93	1.0877930582485988E-5
94-95	5.4948164648878485E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	149.0
22-23	316.0
24-25	363.0
26-27	1208.0
28-29	3412.0
30-31	10146.0
32-33	12578.0
34-35	17679.0
36-37	28252.0
38-39	33024.0
40-41	32682.0
42-43	36418.0
44-45	42703.0
46-47	43687.0
48-49	41490.0
50-51	38180.0
52-53	36343.0
54-55	37989.0
56-57	38685.0
58-59	40820.0
60-61	40321.0
62-63	39869.0
64-65	42032.0
66-67	43971.0
68-69	44470.0
70-71	43826.0
72-73	41195.0
74-75	41524.0
76-77	43425.0
78-79	44595.0
80-81	44284.0
82-83	42459.0
84-85	42025.0
86-87	42876.0
88-89	45087.0
90-91	46108.0
92-93	45816.0
94-95	54123.0
96-97	177840.0
98-99	396053.0
100-101	3934034.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.38783767009086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17943010978294	77.90405969505233
2	7.091515606895666	12.252413981668374
3	1.5803678946539266	4.0957369542716
4	0.5672218288291493	1.9600426908729847
5	0.2526745691111645	1.0914004829867716
6	0.11653587967914641	0.6040369593878004
7	0.06325278551358174	0.3824989956989861
8	0.03893865877498549	0.26910612266756045
9	0.024592970992784582	0.1912080227354935
>10	0.08360854502870382	1.0607072878635482
>50	0.0012832179730823915	0.07285921317528071
>100	5.184857900702669E-4	0.08331919654044809
>500	5.944697475478087E-5	0.032610397078833245
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7264910192253408E-5	0.0	0.0	0.0
3	0.0	1.7264910192253408E-5	0.0	0.0	0.0
4	0.0	3.4529820384506816E-5	0.0	0.0	0.0
5	0.0	3.4529820384506816E-5	0.0	0.0	0.0
6	5.179473057676022E-5	5.179473057676022E-5	0.0	1.7264910192253408E-5	0.0
7	5.179473057676022E-5	5.179473057676022E-5	0.0	1.7264910192253408E-5	0.0
8	5.179473057676022E-5	5.179473057676022E-5	0.0	1.7264910192253408E-5	0.0
9	5.179473057676022E-5	1.0358946115352044E-4	0.0	1.7264910192253408E-5	1.7264910192253408E-5
10-11	5.179473057676022E-5	1.0358946115352044E-4	0.0	3.4529820384506816E-5	1.7264910192253408E-5
12-13	5.179473057676022E-5	1.2085437134577385E-4	0.0	6.905964076901363E-5	1.7264910192253408E-5
14-15	5.179473057676022E-5	1.7264910192253409E-4	0.0	8.632455096126704E-5	5.179473057676022E-5
16-17	6.905964076901363E-5	1.7264910192253409E-4	0.0	1.3811928153802726E-4	6.905964076901363E-5
18-19	6.905964076901363E-5	1.9854646721091418E-4	0.0	1.7264910192253409E-4	7.769209586514034E-5
20-21	6.905964076901363E-5	2.417087426915477E-4	0.0	1.8991401211478748E-4	8.632455096126704E-5
22-23	6.905964076901363E-5	2.6760610797992783E-4	0.0	2.4170874269154773E-4	9.495700605739374E-5
24-25	6.905964076901363E-5	3.107683834605614E-4	0.0	2.848710181721812E-4	1.0358946115352044E-4
26-27	6.905964076901363E-5	3.4529820384506817E-4	0.0	3.280332936528147E-4	1.0358946115352044E-4
28-29	6.905964076901363E-5	3.7982802422957497E-4	0.0	5.870069465366158E-4	1.0358946115352044E-4
30-31	6.905964076901363E-5	5.179473057676023E-4	0.0	0.0015365770071105534	1.0358946115352044E-4
32-33	6.905964076901363E-5	5.265797608637289E-4	0.0	0.003349392577297161	1.0358946115352044E-4
34-35	6.905964076901363E-5	6.38801677113376E-4	0.0	0.0054816089860404565	1.0358946115352044E-4
36-37	6.905964076901363E-5	7.423911382668965E-4	0.0	0.009642452342373528	1.0358946115352044E-4
38-39	6.905964076901363E-5	7.510235933630232E-4	0.0	0.019259007319458676	1.0358946115352044E-4
40-41	6.905964076901363E-5	8.287156892281635E-4	0.0	0.029626585889906847	1.0358946115352044E-4
42-43	6.905964076901363E-5	8.373481443242903E-4	0.0	0.03621314912825152	1.1222191624964714E-4
44-45	6.905964076901363E-5	8.805104198049238E-4	0.0	0.044146375361591966	1.2085437134577385E-4
46-47	6.905964076901363E-5	9.236726952855574E-4	0.0	0.05283062518829543	1.2085437134577385E-4
48-49	6.905964076901363E-5	9.32305150381684E-4	0.0	0.060945132978654525	1.2085437134577385E-4
50-51	6.905964076901363E-5	9.668349707661908E-4	0.0	0.06913733286487878	1.2085437134577385E-4
52-53	6.905964076901363E-5	9.840998809584442E-4	0.0	0.09097744425807933	1.3811928153802726E-4
54-55	6.905964076901363E-5	9.92732336054571E-4	0.0	0.11879121457779956	1.5538419173028066E-4
56-57	6.905964076901363E-5	0.0010013647911506977	0.0	0.1644396371261176	1.5538419173028066E-4
58-59	6.905964076901363E-5	0.0010013647911506977	0.0	0.1971911717608223	1.5538419173028066E-4
60-61	6.905964076901363E-5	0.0010099972462468245	0.0	0.20845652566126766	1.5538419173028066E-4
62-63	6.905964076901363E-5	0.0010704244319197113	0.0	0.2178745341711419	1.5538419173028066E-4
64-65	6.905964076901363E-5	0.0011740138930732317	0.0	0.22214759944372459	1.5538419173028066E-4
66-67	6.905964076901363E-5	0.0011740138930732317	0.0	0.22729254268101612	1.640166468264074E-4
68-69	6.905964076901363E-5	0.0011999112583616117	0.0	0.23203176052878965	1.8128155701866078E-4
70-71	7.769209586514034E-5	0.0012085437134577385	0.0	0.23608901442396923	1.8991401211478748E-4
72-73	1.5538419173028066E-4	0.0012085437134577385	0.0	0.23945567191145864	1.8991401211478748E-4
74-75	1.5538419173028066E-4	0.0012344410787461188	0.0	0.24220942508712306	1.8991401211478748E-4
76-77	1.640166468264074E-4	0.0012862358093228788	0.0	0.24485958880163394	1.8991401211478748E-4
78-79	1.7264910192253409E-4	0.0012948682644190056	0.0	0.24670693419220507	1.8991401211478748E-4
80-81	1.7264910192253409E-4	0.0013035007195151323	0.0	0.24736300077951068	1.8991401211478748E-4
82-83	1.7264910192253409E-4	0.0013207656297073856	0.0	0.2478464182648938	1.8991401211478748E-4
84-85	1.7264910192253409E-4	0.0013466629949957657	0.0	0.24806222964229696	1.8991401211478748E-4
86-87	1.7264910192253409E-4	0.0013639279051880192	0.0	0.24816581910345048	1.8991401211478748E-4
88	1.7264910192253409E-4	0.0013811928153802727	0.0	0.24818308401364272	1.8991401211478748E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6920	0.0	47.806927	1
GTATCAA	12700	0.0	39.548553	1
TCAACGC	14705	0.0	33.218784	4
ATCAACG	14755	0.0	33.077023	3
CAACGCA	15065	0.0	32.424976	5
TATCAAC	15810	0.0	31.278488	2
AACGCAG	15720	0.0	31.23592	6
ACGCAGA	17980	0.0	27.333672	7
CGCAGAG	18160	0.0	27.015305	8
GCAGAGT	21775	0.0	22.550112	9
GTACATG	15525	0.0	21.225874	1
TACATGG	15470	0.0	20.74444	2
GAGTACT	12745	0.0	20.649206	12-13
ACATGGG	15655	0.0	19.948978	3
CAGAGTA	21040	0.0	19.212795	10-11
AGAGTAC	19295	0.0	19.131016	10-11
GTGGTAT	3695	0.0	19.119	1
CATGGGG	12370	0.0	18.595474	4
GTACTTT	14290	0.0	18.37153	14-15
TGGTATC	3595	0.0	17.613844	2
>>END_MODULE