FastQCFastQC Report
Fri 27 May 2016
SRR2138855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5943326
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95950.045.1890221
GTATCAA177350.035.4885331
CGAGACC39400.031.54927486-87
GAGACCG39700.031.32343788-89
ATCAACG200200.030.7103823
TCAACGC202850.030.2880674
CAACGCA204600.030.0499465
AACGCAG211000.029.2375496
ACGAGAC42850.029.00913486-87
GACCGAG43600.028.88905790-91
ACCGAGG44000.028.69047290-91
GCCCACG41750.028.5651582-83
CCCACGA42900.028.30030682-83
TATCAAC220350.028.2714962
ATCTCCG40600.028.20897574-75
CACGAGA44400.028.18241784-85
TCTCCGA41850.028.10771874-75
AGACCGA44800.028.06739288-89
TCCGAGC43050.027.675476-77
CGAGCCC43200.027.64537878-79
Produced by FastQC (version 0.11.5)