FastQCFastQC Report
Fri 27 May 2016
SRR2138854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626771
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.048.1822431
GTATCAA82800.039.4018171
ATCAACG97750.032.799453
TCAACGC98750.032.598934
CAACGCA100550.031.9291785
TATCAAC102900.031.4105172
ATCTCGT52550.031.10192794
AACGCAG104000.030.9516036
ACGCAGA118500.027.0911567
CGCAGAG119400.026.9232358
GTGGTAT20800.025.829141
TATCTCG63150.025.16806894
TGGTATC21000.023.7263072
GCAGAGT141700.022.4416039
TACATGG107450.022.3385492
GTACATG112950.021.6343821
ACATGGG110250.021.103153
ACCGAAC5300.020.4358088
GAGTACT81500.019.82812-13
CATGGGG81700.019.3563044
Produced by FastQC (version 0.11.5)