##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3626771
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.779835285988554	32.0	30.0	33.0	18.0	34.0
2	31.603859741902646	33.0	32.0	33.0	27.0	34.0
3	32.283770604761095	33.0	33.0	33.0	30.0	34.0
4	32.342103209714644	33.0	33.0	33.0	31.0	34.0
5	32.99085825931662	33.0	33.0	34.0	32.0	34.0
6	36.935582919351674	38.0	37.0	38.0	35.0	38.0
7	37.312934839282654	38.0	38.0	38.0	36.0	38.0
8	37.28140238244984	38.0	38.0	38.0	36.0	38.0
9	37.42049167151717	38.0	38.0	38.0	37.0	38.0
10-11	37.48421033475783	38.0	38.0	38.0	37.0	38.0
12-13	37.498380101748914	38.0	38.0	38.0	37.0	38.0
14-15	37.494929235951204	38.0	38.0	38.0	37.0	38.0
16-17	37.49373478501951	38.0	38.0	38.0	37.0	38.0
18-19	37.4953156678489	38.0	38.0	38.0	37.0	38.0
20-21	37.49694729981249	38.0	38.0	38.0	37.0	38.0
22-23	37.50506003923138	38.0	38.0	38.0	37.0	38.0
24-25	37.467377505894916	38.0	38.0	38.0	37.0	38.0
26-27	37.47546640309108	38.0	38.0	38.0	37.0	38.0
28-29	37.47187457168947	38.0	38.0	38.0	37.0	38.0
30-31	37.46460115604806	38.0	38.0	38.0	37.0	38.0
32-33	37.46043294582447	38.0	38.0	38.0	37.0	38.0
34-35	37.444109552881955	38.0	38.0	38.0	37.0	38.0
36-37	37.420213969962795	38.0	38.0	38.0	37.0	38.0
38-39	37.39266540861255	38.0	38.0	38.0	37.0	38.0
40-41	37.38698322119476	38.0	38.0	38.0	37.0	38.0
42-43	37.37076729388651	38.0	38.0	38.0	37.0	38.0
44-45	37.339601708327244	38.0	38.0	38.0	37.0	38.0
46-47	37.304701904180924	38.0	38.0	38.0	37.0	38.0
48-49	37.2625316492201	38.0	38.0	38.0	37.0	38.0
50-51	37.241527465100674	38.0	38.0	38.0	37.0	38.0
52-53	37.23110477708899	38.0	38.0	38.0	37.0	38.0
54-55	37.20960509695779	38.0	38.0	38.0	37.0	38.0
56-57	37.173776290291386	38.0	38.0	38.0	37.0	38.0
58-59	37.177479973705815	38.0	38.0	38.0	37.0	38.0
60-61	37.18714330416904	38.0	38.0	38.0	37.0	38.0
62-63	37.191178989078466	38.0	38.0	38.0	37.0	38.0
64-65	37.1934761236903	38.0	38.0	38.0	37.0	38.0
66-67	37.19703538087663	38.0	38.0	38.0	36.5	38.0
68-69	37.194280525475996	38.0	38.0	38.0	36.0	38.0
70-71	37.16024004392642	38.0	38.0	38.0	36.0	38.0
72-73	37.13363968719342	38.0	38.0	38.0	36.0	38.0
74-75	37.11525210109728	38.0	38.0	38.0	36.0	38.0
76-77	37.11501244709679	38.0	38.0	38.0	36.0	38.0
78-79	37.11636856102578	38.0	38.0	38.0	36.0	38.0
80-81	37.10359808630821	38.0	38.0	38.0	36.0	38.0
82-83	37.081888854009264	38.0	38.0	38.0	36.0	38.0
84-85	37.09862860373653	38.0	38.0	38.0	36.0	38.0
86-87	37.06955219240862	38.0	38.0	38.0	36.0	38.0
88-89	37.028583038628405	38.0	38.0	38.0	36.0	38.0
90-91	37.03328151859105	38.0	38.0	38.0	36.0	38.0
92-93	37.0118675492635	38.0	38.0	38.0	36.0	38.0
94-95	36.973300248788945	38.0	38.0	38.0	36.0	38.0
96-97	36.91814320676396	38.0	38.0	38.0	35.0	38.0
98-99	36.97473201932462	38.0	38.0	38.0	35.0	38.0
100	35.258103354755995	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	15.0
20	44.0
21	126.0
22	313.0
23	700.0
24	1387.0
25	2631.0
26	4636.0
27	7354.0
28	11437.0
29	17368.0
30	23898.0
31	32179.0
32	41783.0
33	55892.0
34	82407.0
35	146960.0
36	433972.0
37	2763662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.763466455422744	19.59613661849618	14.041112604021594	23.599284322059486
2	14.138637371921195	20.47223273815744	43.34230090623312	22.046828983688243
3	18.108835655738947	26.384296113540117	30.305607936095218	25.201260294625715
4	12.190072105462407	18.317781850577276	38.962647490012465	30.52949855394785
5	13.258119309314113	37.62255483187832	35.764861272728275	13.354464586079292
6	29.58469933301533	37.947622815894036	19.05177334101694	13.4159045100737
7	26.278699779693778	32.7624856277073	23.11094996374184	17.84786462885708
8	23.833459570510517	37.288017357588885	21.40954033215772	17.468982739742874
9	25.314839374981048	17.247137259875867	21.53613270210132	35.90189066304177
10-11	24.042091993413976	26.9292335977697	29.02642998778114	20.002244421035186
12-13	24.87496453456808	24.36692308392231	29.18295640943418	21.575155972075436
14-15	21.958954673454706	25.80857738191907	26.858560962354666	25.37390698227156
16-17	21.560018539907812	29.009027589555558	27.51486101548733	21.9160928550493
18-19	21.55801951653413	28.04258939977186	29.34818603104525	21.05120505264876
20-21	22.33990560360545	27.169342058370805	29.20143572959369	21.28931660843005
22-23	22.058605168909775	27.242652984158415	29.061630433029272	21.637111413902538
24-25	21.873252408711622	27.300395431306907	29.198189950419422	21.628162209562046
26-27	21.839894631670013	27.44402652188817	29.14513433993255	21.570944506509267
28-29	21.70856845561899	27.429667448629875	29.174133201510372	21.687630894240765
30-31	21.91416738837896	27.164325778513916	29.224467503342105	21.697039329765015
32-33	21.631011932934506	27.474111366399928	29.103155944420976	21.791720756244587
34-35	21.864682164898912	27.393210580112736	29.190962422687335	21.551144832301016
36-37	21.740690462851973	27.41278537718832	29.223114378750857	21.62340978120885
38-39	21.607748856116977	27.3928784887313	29.194270399620347	21.80510225553138
40-41	21.94697910965896	27.189489527831384	29.264237137796606	21.59929422471305
42-43	21.872818190528854	27.212550295285997	29.105120739115847	21.809510775069306
44-45	21.76608969064167	27.418986816105434	29.012055003627967	21.802868489624927
46-47	21.93290398837356	27.29512675343912	28.846877034763363	21.92509222342396
48-49	21.851112800984513	27.48413808716651	28.71930654561497	21.945442566234007
50-51	21.659176131898967	27.661276232601956	28.574114737023553	22.105432898475524
52-53	21.74642276310651	27.456622637706275	28.68041922525891	22.11653537392831
54-55	21.927134319470685	27.22910046726914	28.79722974056198	22.0465354726982
56-57	21.948982564481362	27.006962131592527	28.70769022385447	22.336365080071637
58-59	21.51936936996534	26.964981969041524	28.993775346502964	22.521873314490172
60-61	21.81702259629868	27.420154792843093	29.022104470076876	21.74071814078135
62-63	21.274431264498432	27.759449132999812	28.951329129723185	22.014790472778568
64-65	21.092973097269272	28.13436797965862	28.586905355263887	22.18575356780822
66-67	21.166003647844185	28.687137657656237	28.137332856514785	22.009525837984796
68-69	21.30051210135315	28.58295191539905	27.964675720820797	22.151860262427
70-71	21.3082784979512	27.900115194645437	28.061440454423014	22.730165852980353
72-73	21.620439443706452	27.225922308497967	28.093716004296187	23.059922243499397
74-75	21.7527822204082	27.30591004172499	28.254664709127685	22.686643028739127
76-77	22.03933927368851	27.2603897976263	27.56036083523631	23.139910093448886
78-79	22.255830990291063	27.34528533822561	27.074292780146607	23.324590891336715
80-81	22.0579675589248	27.65993152600653	27.32607473558187	22.9560261794868
82-83	22.06150102104168	27.48266650589605	27.32161585672702	23.13421661633525
84-85	22.20752812615739	27.82173348830874	27.208594981836843	22.762143403697024
86-87	22.636625937744228	27.722585895305812	27.30901621533029	22.331771951619668
88-89	22.393851145641584	27.90678265684084	27.592341038795553	22.107025158722028
90-91	22.37803821326756	27.47513470362802	27.425526673915314	22.72130040918911
92-93	22.371427626121257	27.102967896249996	27.61877656496059	22.906827912668152
94-95	21.64506698114959	27.593004272769335	28.23400723698336	22.527921509097716
96-97	21.775088474329106	27.448684889118102	28.44859597019438	22.327630666358413
98-99	22.900157651308714	29.34528111389911	28.803400658987883	18.951160575804295
100	21.97698022649945	30.327133910813785	22.449785499432927	25.24610036325383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	464.0
1	479.0
2	773.5
3	1204.0
4	1609.5
5	2197.0
6	2697.5
7	3373.5
8	4303.5
9	4894.5
10	5010.0
11	5038.0
12	5502.0
13	6570.0
14	8595.5
15	10855.0
16	12304.5
17	13061.0
18	12889.0
19	11887.0
20	10841.0
21	10260.5
22	10680.0
23	12445.0
24	15668.5
25	20517.5
26	27135.0
27	34763.5
28	42444.0
29	51031.0
30	59699.0
31	68369.5
32	77263.0
33	85632.0
34	94125.0
35	101709.5
36	110101.0
37	118765.0
38	124368.0
39	128766.0
40	134768.5
41	140101.0
42	143658.5
43	146849.0
44	153233.0
45	161450.5
46	160287.5
47	155769.0
48	153027.0
49	147985.5
50	143398.0
51	138393.5
52	131615.5
53	124039.5
54	116323.0
55	107459.0
56	98034.5
57	87758.5
58	77246.5
59	67412.5
60	56195.0
61	44553.5
62	34290.5
63	25963.5
64	19590.5
65	14296.0
66	10421.0
67	8002.0
68	6049.0
69	4321.0
70	3103.0
71	2249.0
72	1568.0
73	1035.0
74	653.5
75	407.5
76	258.0
77	150.0
78	91.5
79	67.0
80	41.0
81	26.0
82	19.5
83	12.5
84	9.5
85	7.0
86	4.5
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.006369302059600675
6	2.7572736188747512E-5
7	2.7572736188747512E-5
8	0.0
9	2.7572736188747512E-5
10-11	1.3786368094373756E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.066552284580864E-4
26-27	0.0
28-29	6.482602144086177E-4
30-31	0.0026506951240877364
32-33	2.4903164735679884E-4
34-35	4.161814107023824E-5
36-37	4.1786291533484467E-4
38-39	6.435699665483524E-4
40-41	6.74947902458779E-4
42-43	1.4153213918893307E-4
44-45	0.0
46-47	0.0013810664422433698
48-49	2.9024534729452153E-5
50-51	0.001946125680796466
52-53	0.0013643031117700189
54-55	0.002525080508962274
56-57	0.0024973788539881013
58-59	0.003336913287588711
60-61	0.0024117367241661197
62-63	0.003013927178446422
64-65	0.004078102740199123
66-67	0.00150396111455368
68-69	1.0744876267377342E-4
70-71	6.051406230434756E-4
72-73	0.006138376912669578
74-75	0.005466064375028419
76-77	0.004669587504311772
78-79	0.0022120693084909876
80-81	1.630876537427312E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0020355087720250534
90-91	2.2284137842134368E-4
92-93	0.0
94-95	2.101529808624188E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	86.0
22-23	174.0
24-25	236.0
26-27	748.0
28-29	2112.0
30-31	7341.0
32-33	9055.0
34-35	12685.0
36-37	20014.0
38-39	12719.0
40-41	22931.0
42-43	25293.0
44-45	29970.0
46-47	30699.0
48-49	28919.0
50-51	14822.0
52-53	2518.0
54-55	2291.0
56-57	2235.0
58-59	15376.0
60-61	28422.0
62-63	28386.0
64-65	30105.0
66-67	33175.0
68-69	34884.0
70-71	38916.0
72-73	29781.0
74-75	28849.0
76-77	29718.0
78-79	30623.0
80-81	30821.0
82-83	29234.0
84-85	28743.0
86-87	29656.0
88-89	30619.0
90-91	30543.0
92-93	30757.0
94-95	35818.0
96-97	120930.0
98-99	272981.0
100-101	2433560.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.59648175715458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.18142885524783	78.09394458697601
2	7.177965451636924	12.431731085723253
3	1.5779406668524096	4.09932330512871
4	0.5283118920887618	1.8299980450140914
5	0.223193830281955	0.9663900226160383
6	0.10712336193237991	0.5565903754405442
7	0.06204761586214064	0.37611736645565574
8	0.0388102439509353	0.26886644658303094
9	0.02507576912441007	0.1954326045175737
>10	0.0763885183654616	0.9621579743129735
>50	9.835022600018577E-4	0.056308064999903415
>100	6.393458135375242E-4	0.11029448300660012
>500	9.094658318867779E-5	0.05284563922554365
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.7572736188747512E-5	0.0	0.0	2.7572736188747512E-5
5	2.7572736188747512E-5	2.7572736188747512E-5	0.0	0.0	2.7572736188747512E-5
6	1.3786368094373754E-4	2.7572736188747512E-5	0.0	0.0	2.7572736188747512E-5
7	1.3786368094373754E-4	5.5145472377495025E-5	0.0	0.0	2.7572736188747512E-5
8	1.3786368094373754E-4	5.5145472377495025E-5	0.0	0.0	2.7572736188747512E-5
9	1.3786368094373754E-4	1.6543641713248505E-4	0.0	0.0	5.5145472377495025E-5
10-11	1.6543641713248505E-4	1.6543641713248505E-4	0.0	0.0	5.5145472377495025E-5
12-13	1.6543641713248505E-4	1.6543641713248505E-4	0.0	0.0	5.5145472377495025E-5
14-15	1.7922278522685884E-4	2.481546256987276E-4	0.0	0.0	5.5145472377495025E-5
16-17	2.757273618874751E-4	2.757273618874751E-4	0.0	0.0	5.5145472377495025E-5
18-19	3.033000980762226E-4	2.757273618874751E-4	0.0	0.0	5.5145472377495025E-5
20-21	3.033000980762226E-4	3.033000980762226E-4	0.0	0.0	5.5145472377495025E-5
22-23	3.033000980762226E-4	3.998046747368389E-4	0.0	0.0	6.893184047186877E-5
24-25	3.033000980762226E-4	4.411637790199602E-4	0.0	0.0	8.271820856624253E-5
26-27	3.033000980762226E-4	4.549501471143339E-4	0.0	5.5145472377495025E-5	1.240773128493638E-4
28-29	3.033000980762226E-4	4.8252288330308143E-4	0.0	2.481546256987276E-4	1.3786368094373754E-4
30-31	3.308728342649701E-4	5.790274599636977E-4	0.0	9.650457666061629E-4	1.3786368094373754E-4
32-33	3.308728342649701E-4	5.790274599636977E-4	0.0	0.0029502827721959836	1.3786368094373754E-4
34-35	3.722319385480914E-4	6.341729323411927E-4	0.0	0.005693770022976361	1.6543641713248505E-4
36-37	4.135910428312127E-4	6.617456685299402E-4	0.0	0.011842490193067056	1.6543641713248505E-4
38-39	4.135910428312127E-4	7.031047728130616E-4	0.0	0.17704453906794776	1.6543641713248505E-4
40-41	4.411637790199602E-4	8.133957175680516E-4	0.0	0.3459826936964038	1.6543641713248505E-4
42-43	4.411637790199602E-4	8.409684537567992E-4	0.0	0.3615475032749517	1.6543641713248505E-4
44-45	4.411637790199602E-4	9.512593985117892E-4	0.0	0.3823097736250786	1.6543641713248505E-4
46-47	4.549501471143339E-4	0.0010477639751724054	0.0	0.4041749534227554	1.7922278522685884E-4
48-49	4.687365152087077E-4	0.0010477639751724054	0.0	0.42575061949045034	1.930091533212326E-4
50-51	4.8252288330308143E-4	0.0010891230794555268	0.0	0.44717463550910713	1.930091533212326E-4
52-53	5.514547237749502E-4	0.0011029094475499004	0.0	0.961764610999702	2.2058188950998007E-4
54-55	5.514547237749502E-4	0.0011166958156442741	0.0	1.640881103328553	2.481546256987276E-4
56-57	5.514547237749502E-4	0.0011580549199273955	0.0	2.371007157606587	2.481546256987276E-4
58-59	5.514547237749502E-4	0.0011718412880217693	0.0	3.1220471322837864	2.481546256987276E-4
60-61	5.514547237749502E-4	0.001240773128493638	0.0	3.318626954941462	2.481546256987276E-4
62-63	6.066001961524452E-4	0.0012545594965880117	0.0	3.3251479070501	2.757273618874751E-4
64-65	6.066001961524452E-4	0.001461355018003618	0.0	3.330951968017832	2.757273618874751E-4
66-67	6.341729323411928E-4	0.001461355018003618	0.0	3.3364940879917704	2.757273618874751E-4
68-69	6.341729323411928E-4	0.0015027141222867394	0.0	3.3414296077695558	2.757273618874751E-4
70-71	6.341729323411928E-4	0.0015165004903811132	0.0	3.3459515365045105	2.757273618874751E-4
72-73	7.031047728130616E-4	0.0015165004903811132	0.0	3.3498255059390294	2.757273618874751E-4
74-75	7.168911409074353E-4	0.0015165004903811132	0.0	3.352651711398376	2.757273618874751E-4
76-77	7.168911409074353E-4	0.0015992186989473556	0.0	3.3559052942686485	2.757273618874751E-4
78-79	7.168911409074353E-4	0.0016267914351361032	0.0	3.357807813065672	2.757273618874751E-4
80-81	7.168911409074353E-4	0.0016267914351361032	0.0	3.3585660633108625	2.757273618874751E-4
82-83	7.168911409074353E-4	0.0016681505394192243	0.0	3.3590210134579768	3.033000980762226E-4
84-85	7.168911409074353E-4	0.0016957232756079719	0.0	3.3592002362432036	3.033000980762226E-4
86-87	7.168911409074353E-4	0.0017370823798910932	0.0	3.3592967408198646	3.033000980762226E-4
88	7.168911409074353E-4	0.0017370823798910932	0.0	3.359324313556053	3.308728342649701E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4595	0.0	48.182243	1
GTATCAA	8280	0.0	39.401817	1
ATCAACG	9775	0.0	32.79945	3
TCAACGC	9875	0.0	32.59893	4
CAACGCA	10055	0.0	31.929178	5
TATCAAC	10290	0.0	31.410517	2
ATCTCGT	5255	0.0	31.101927	94
AACGCAG	10400	0.0	30.951603	6
ACGCAGA	11850	0.0	27.091156	7
CGCAGAG	11940	0.0	26.923235	8
GTGGTAT	2080	0.0	25.82914	1
TATCTCG	6315	0.0	25.168068	94
TGGTATC	2100	0.0	23.726307	2
GCAGAGT	14170	0.0	22.441603	9
TACATGG	10745	0.0	22.338549	2
GTACATG	11295	0.0	21.634382	1
ACATGGG	11025	0.0	21.10315	3
ACCGAAC	530	0.0	20.435808	8
GAGTACT	8150	0.0	19.828	12-13
CATGGGG	8170	0.0	19.356304	4
>>END_MODULE