FastQCFastQC Report
Fri 27 May 2016
SRR2138853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7933686
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112150.051.949741
GTATCAA207250.038.988341
TCAACGC260050.030.6140444
ATCAACG261000.030.4860123
TATCAAC266600.030.0000762
CAACGCA268000.029.6897345
AACGCAG275450.028.9968626
ACGCAGA316600.025.2280037
CGCAGAG321650.024.8049688
GCAGAGT365850.021.7968719
GTACATG259950.021.195281
GTGGTAT57650.021.0543631
TACATGG261800.020.4992122
GAGTACT210300.020.23712712-13
TGGTATC55000.019.783232
ACATGGG268950.019.6910133
AGAGTAC333450.019.45013810-11
CAGAGTA358900.019.03694210-11
GTACTTT230650.018.28263514-15
AGTACTT229700.017.69776712-13
Produced by FastQC (version 0.11.5)