FastQCFastQC Report
Fri 27 May 2016
SRR2138852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045597
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.038.249511
GTATCAA95550.031.9501441
TCAACGC97900.029.6618564
ATCAACG99150.029.2030643
CAACGCA102600.028.3024755
AACGCAG106400.027.209336
TATCAAC113500.026.1680952
ACGCAGA128200.022.549267
CGCAGAG133250.021.6617978
GTACATG209750.019.6071831
TACATGG206550.019.172622
ACATGGG209850.018.0785393
GCAGAGT168900.016.9347159
GTGTAGA102850.016.67659494
CATGGGG151650.016.6185134
TAGCGCG2500.00262113715.83250194
AGAGTAC144300.015.34930610-11
CAGAGTA164000.015.07407310-11
TGTAGAT107750.014.32639394
TATACTG42350.014.207685
Produced by FastQC (version 0.11.5)