##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7045597
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85461672020128	33.0	31.0	34.0	30.0	34.0
2	32.12379845171389	34.0	31.0	34.0	30.0	34.0
3	32.291437475064214	34.0	31.0	34.0	30.0	34.0
4	35.715177578280446	37.0	35.0	37.0	35.0	37.0
5	35.776690605494466	37.0	35.0	37.0	35.0	37.0
6	35.80272757581792	37.0	36.0	37.0	35.0	37.0
7	35.72962376360726	37.0	36.0	37.0	35.0	37.0
8	35.67815019791794	37.0	35.0	37.0	35.0	37.0
9	37.42065264306204	39.0	38.0	39.0	35.0	39.0
10-11	37.414034822031404	39.0	38.0	39.0	35.0	39.0
12-13	37.33870621609496	39.0	38.0	39.0	34.5	39.0
14-15	38.75012323299218	41.0	39.0	41.0	35.0	41.0
16-17	38.75683700046994	41.0	39.0	41.0	34.0	41.0
18-19	38.77604034122304	41.0	38.5	41.0	34.0	41.0
20-21	38.80815755638329	40.5	39.0	41.0	34.0	41.0
22-23	38.77653110924052	40.0	39.0	41.0	34.0	41.0
24-25	38.73741629780966	40.0	38.0	41.0	34.0	41.0
26-27	38.68350504715422	40.0	38.0	41.0	34.0	41.0
28-29	38.60620515583643	40.0	38.0	41.0	34.0	41.0
30-31	38.48583615836131	40.0	38.0	41.0	34.0	41.0
32-33	38.415327682358466	40.0	38.0	41.0	33.5	41.0
34-35	38.25629681829207	40.0	38.0	41.0	33.0	41.0
36-37	38.14972116917234	40.0	38.0	41.0	33.0	41.0
38-39	38.139363704826394	40.0	38.0	41.0	33.0	41.0
40-41	38.05131414162112	40.0	38.0	41.0	33.0	41.0
42-43	37.884304989741466	40.0	37.5	41.0	32.5	41.0
44-45	37.951928393670556	40.0	37.5	41.0	32.5	41.0
46-47	38.15474081524416	40.0	38.0	41.0	33.0	41.0
48-49	38.148308222847305	40.0	38.0	41.0	33.0	41.0
50-51	38.05667063306335	40.0	37.5	41.0	33.0	41.0
52-53	37.9833845745731	40.0	37.0	41.0	33.0	41.0
54-55	37.905808363970344	40.0	37.0	41.0	33.0	41.0
56-57	37.95907166242128	40.0	37.0	41.0	33.0	41.0
58-59	37.77027857562692	40.0	36.5	41.0	32.0	41.0
60-61	37.69343299832023	39.5	36.0	41.0	32.0	41.0
62-63	37.46692234559873	39.0	36.0	41.0	32.0	41.0
64-65	37.26489920138376	39.0	35.0	41.0	32.0	41.0
66-67	36.99279525864285	39.0	35.0	41.0	31.0	41.0
68-69	36.755665579616704	38.0	35.0	40.0	31.0	41.0
70-71	36.4605893854053	37.0	35.0	40.0	31.0	41.0
72-73	36.12389642325776	37.0	35.0	39.5	31.0	41.0
74-75	35.7791553405887	36.0	34.5	39.0	31.0	41.0
76-77	35.44172253472829	36.0	34.0	39.0	30.5	40.5
78-79	35.07401221218319	35.0	34.0	37.5	30.0	39.5
80-81	34.74912706547718	35.0	34.0	37.0	30.0	39.0
82-83	34.42665320023925	35.0	34.0	36.5	30.0	39.0
84-85	34.19890969793239	35.0	34.0	36.0	30.0	37.5
86-87	33.954514098723116	35.0	34.0	36.0	30.0	37.0
88-89	33.70353881498369	35.0	34.0	35.5	29.0	37.0
90-91	33.51893696587106	35.0	33.0	35.0	29.0	36.0
92-93	33.423695474915945	35.0	33.0	35.0	29.0	36.0
94-95	33.40191316773115	35.0	33.0	35.0	29.0	36.0
96-97	33.3678241089455	35.0	33.0	35.0	29.0	36.0
98-99	33.420047281723726	35.0	33.0	35.0	29.0	35.5
100	32.82297487342757	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	9.0
16	40.0
17	186.0
18	992.0
19	2959.0
20	6559.0
21	12483.0
22	19523.0
23	27325.0
24	36627.0
25	47913.0
26	56785.0
27	63018.0
28	72417.0
29	89018.0
30	104616.0
31	126330.0
32	162376.0
33	218183.0
34	308139.0
35	454811.0
36	735854.0
37	1319705.0
38	1953370.0
39	1158518.0
40	67840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08093324100144	20.489094110832625	14.695191904958516	23.734780743207423
2	14.1324378365832	21.07516394844789	41.90764373482337	22.88475448014554
3	17.807799938734277	26.236691809840046	30.44614109683817	25.509367154587505
4	12.315401337973032	18.488339149990264	39.519074035908275	29.677185476128425
5	13.439922304563387	38.151914337431215	35.50049510684542	12.907668251159981
6	29.451460957665105	38.173487800086534	19.403509001034497	12.971542241213871
7	26.316824867377537	32.945300995333824	23.31205932970211	17.425814807586537
8	24.058269758315095	36.91701213832644	21.648659935233113	17.376058168125347
9	24.895049290212985	18.04883941036835	21.6502640374768	35.40584726194187
10-11	23.60006015332569	27.42513320349318	29.272023402170188	19.702783241010945
12-13	24.358812395900962	24.831873048786267	29.159830824074014	21.649483731238753
14-15	21.72923597777616	26.463710821524984	26.561727535535763	25.245325665163094
16-17	21.66229509569564	29.436152152670175	27.23564533478659	21.665907416847595
18-19	21.708466030006516	29.043591788625537	28.329432097272427	20.91851008409552
20-21	22.597423695233072	28.172773907047176	28.037961813251417	21.19184058446833
22-23	22.291885058735478	27.99503185131534	27.876711131611604	21.836371958337576
24-25	22.056825400120843	28.13880013588701	27.923188288120848	21.881186175871296
26-27	22.02621250410291	28.32347780984192	27.78548066743131	21.864829018623865
28-29	21.932162464811178	28.417670377157478	27.838127932639225	21.81203922539212
30-31	22.286125161671823	28.188316706677945	27.759237125900256	21.76632100574998
32-33	22.04870613909378	28.289717171945316	27.733778903536493	21.92779778542441
34-35	22.191647915797056	28.285934243886214	27.77748845488226	21.74492938543447
36-37	22.144302262831353	28.12317708140616	27.84293393913655	21.889586716625935
38-39	21.916537592796303	28.225009029940885	27.97707610550318	21.881377271759632
40-41	22.154424621333167	28.08399617691964	27.924080406423297	21.8374987953239
42-43	22.009453381075215	28.07185605396878	27.888245187679427	22.03044537727658
44-45	22.032159128517623	28.117552551814125	27.902479254656154	21.9478090650121
46-47	21.967515426521807	28.03465877139014	27.849506721503463	22.14831908058459
48-49	21.8335393340737	28.0285452082164	28.07126490266788	22.066650555042024
50-51	21.74470816527604	28.058629112312637	28.077837425730017	22.1188252966813
52-53	21.83835920625828	27.933256240999206	28.027569432175696	22.20081512056682
54-55	21.906407611806507	27.711166070869286	28.138955912731916	22.243470404592287
56-57	21.816176921004427	27.651565882146922	28.123681929160654	22.408575267687993
58-59	21.697299185897815	27.647173897719306	28.280832100983446	22.374694815399433
60-61	21.691888346915622	27.43244583338202	28.534667863861348	22.34099795584101
62-63	21.581550110455222	27.62711784142931	28.52963423908194	22.261697809033524
64-65	21.41069490495585	27.87318322913823	28.30231831003121	22.413803555874708
66-67	21.334077651351947	28.093764190601178	28.017295989284513	22.55486216876236
68-69	21.37966326182497	28.223001437165312	27.99398891329617	22.403346387713547
70-71	21.413098657253354	28.203477652867203	27.76710720386092	22.61631648601852
72-73	21.637683914777302	28.085729790267195	27.5051252035366	22.7714610914189
74-75	21.775766669159275	27.96488174596029	27.53739843258238	22.721953152298063
76-77	21.93840079477458	27.62318608543891	27.629240195533306	22.809172924253208
78-79	22.223026822159753	27.455895858934294	27.545236218589924	22.775841100316033
80-81	22.516104584447678	27.429641305265523	27.20755753350269	22.846696576784105
82-83	22.260311089744995	27.48314592546639	27.356644784130207	22.899898200658406
84-85	22.38606988697958	27.593885362536554	27.34685326864046	22.67319148184341
86-87	22.13745291406503	27.83153264094335	27.492263882110805	22.538750562880814
88-89	21.94118462671693	27.971783872784783	27.490506521079773	22.59652497941852
90-91	21.792563036150344	28.06458082631368	27.79299582691184	22.349860310624134
92-93	21.797795795304566	27.744902569933377	28.152357303020263	22.30494433174179
94-95	21.47214529838751	27.77504288790777	28.512416329210183	22.24039548449454
96-97	21.78362293195596	27.729714468923294	28.51058615715066	21.97607644197009
98-99	23.177223882115282	29.592147406250184	28.838789371751115	18.39183933988341
100	22.265886795738147	30.888521872776337	22.56441542220477	24.28117590928074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2166.0
1	1684.0
2	1343.0
3	1464.5
4	1459.5
5	1618.5
6	1971.5
7	2378.5
8	2733.0
9	3080.0
10	3348.5
11	3501.0
12	3744.5
13	4196.5
14	4884.5
15	5817.0
16	6887.0
17	8162.5
18	9663.0
19	11442.5
20	13748.0
21	16996.0
22	21684.0
23	27695.0
24	35741.0
25	46276.0
26	59440.0
27	74559.5
28	89730.0
29	105755.5
30	121624.5
31	137851.5
32	155312.5
33	171576.0
34	187572.5
35	202850.0
36	219630.5
37	235741.0
38	246606.5
39	254841.5
40	265185.0
41	274888.0
42	281645.5
43	287371.0
44	291585.5
45	295539.0
46	296489.0
47	295779.0
48	294566.0
49	288908.0
50	280168.0
51	268699.0
52	253738.0
53	237556.5
54	220374.5
55	201647.0
56	182488.0
57	163374.5
58	144680.0
59	126137.5
60	106615.5
61	87686.0
62	69891.5
63	54295.0
64	41651.0
65	31916.5
66	24786.0
67	19438.5
68	15292.0
69	11827.0
70	9207.5
71	7167.5
72	5535.5
73	4197.0
74	3239.5
75	2604.5
76	2058.0
77	1601.0
78	1277.5
79	1008.0
80	770.0
81	590.0
82	458.0
83	317.5
84	229.5
85	182.5
86	155.0
87	130.0
88	88.0
89	58.5
90	42.0
91	32.5
92	27.0
93	19.5
94	14.0
95	10.0
96	8.0
97	8.0
98	6.0
99	8.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02075054817923875
3	0.012419103732444533
4	0.004442490820863016
5	0.009268199699755748
6	0.008927561425951556
7	0.0031509040326887843
8	0.009069494040036635
9	0.005464405642275594
10-11	0.0037966974267759
12-13	0.008842401857500506
14-15	0.005251506721147973
16-17	0.003768310903958884
18-19	0.005592144994952167
20-21	0.0044516469141105495
22-23	0.010493086249687854
24-25	0.009739800864439319
26-27	0.023330966083435208
28-29	0.019773214901083355
30-31	0.015615373031573086
32-33	0.012913997422489037
34-35	0.008695645322534307
36-37	0.010452791958692292
38-39	0.005244196326552282
40-41	0.0061661121633916835
42-43	0.0041385833360123815
44-45	0.00740191368331062
46-47	0.010025279126906154
48-49	0.007579917709871927
50-51	0.010478186462479713
52-53	0.001048409061555665
54-55	0.003113268684099531
56-57	6.043550270922659E-4
58-59	0.0028554468882767536
60-61	0.001048582945008116
62-63	0.007463598609019725
64-65	0.0035463825155461134
66-67	0.004105763237684086
68-69	0.005248233737807504
70-71	8.260686054652699E-4
72-73	5.980548737380255E-4
74-75	0.0
76-77	4.014660254558366E-5
78-79	5.682680270245543E-4
80-81	1.888952739141622E-4
82-83	0.0
84-85	9.843495157589308E-4
86-87	0.0033193612968616592
88-89	0.021937211749196328
90-91	0.002762043609677849
92-93	0.0014033629940508135
94-95	0.0013107880220918757
96-97	4.576201696556735E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2251.0
20-21	4161.0
22-23	4861.0
24-25	5578.0
26-27	7059.0
28-29	9186.0
30-31	12809.0
32-33	14584.0
34-35	19195.0
36-37	26788.0
38-39	30914.0
40-41	27820.0
42-43	28984.0
44-45	33551.0
46-47	39906.0
48-49	25411.0
50-51	25275.0
52-53	11109.0
54-55	11715.0
56-57	12313.0
58-59	13011.0
60-61	13717.0
62-63	52959.0
64-65	57652.0
66-67	61080.0
68-69	62080.0
70-71	62474.0
72-73	61502.0
74-75	63802.0
76-77	67290.0
78-79	70456.0
80-81	72410.0
82-83	72015.0
84-85	76252.0
86-87	93214.0
88-89	85715.0
90-91	89329.0
92-93	97277.0
94-95	120673.0
96-97	268091.0
98-99	490702.0
100-101	4640426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.75468572755383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90141125746462	77.09467268847449
2	7.096048597619429	12.170388347926067
3	1.7279628552279835	4.44542734796887
4	0.608730829366004	2.0880608385981945
5	0.27954450829968225	1.1986125728051407
6	0.14243313444076575	0.7328585208694974
7	0.07596322411380779	0.4559941687511929
8	0.044725919344632825	0.3068365725819927
9	0.03387401898931707	0.2614370267482269
>10	0.08792563396400405	1.0976123293052744
>50	9.365614189594487E-4	0.0510414696870115
>100	3.9420813409510346E-4	0.05402007152272117
>500	2.4625808819747342E-5	0.016788639044187842
>1k	2.462580789123579E-5	0.026249405717095504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.257978422552411E-5	0.0	0.0	0.0
3	0.0	4.257978422552411E-5	0.0	1.4193261408508038E-5	0.0
4	0.0	9.935282985955626E-5	0.0	2.8386522817016075E-5	0.0
5	0.0	9.935282985955626E-5	0.0	4.257978422552411E-5	0.0
6	1.4193261408508038E-5	9.935282985955626E-5	0.0	4.257978422552411E-5	0.0
7	2.8386522817016075E-5	1.135460912680643E-4	0.0	5.677304563403215E-5	0.0
8	2.8386522817016075E-5	1.4193261408508036E-4	0.0	5.677304563403215E-5	1.4193261408508038E-5
9	2.8386522817016075E-5	2.1289892112762054E-4	0.0	8.515956845104823E-5	2.8386522817016075E-5
10-11	2.8386522817016075E-5	2.1289892112762054E-4	0.0	8.515956845104823E-5	2.8386522817016075E-5
12-13	2.8386522817016075E-5	2.1289892112762054E-4	0.0	1.0644946056381029E-4	2.8386522817016075E-5
14-15	2.8386522817016075E-5	2.696719667616527E-4	0.0	1.135460912680643E-4	2.8386522817016075E-5
16-17	4.257978422552411E-5	2.838652281701607E-4	0.0	1.135460912680643E-4	4.257978422552411E-5
18-19	4.257978422552411E-5	2.9096185887441474E-4	0.0	1.2773935267657233E-4	4.257978422552411E-5
20-21	4.257978422552411E-5	3.335416430999389E-4	0.0	1.6322250619784244E-4	4.257978422552411E-5
22-23	4.257978422552411E-5	3.5483153521270093E-4	0.0	2.696719667616527E-4	4.9676414929778135E-5
24-25	4.257978422552411E-5	3.76121427325463E-4	0.0	5.819237177488295E-4	5.677304563403215E-5
26-27	4.257978422552411E-5	3.90314688733971E-4	0.0	0.0014335194022593117	5.677304563403215E-5
28-29	4.257978422552411E-5	4.0450795014247904E-4	0.0	0.007075340812141256	5.677304563403215E-5
30-31	4.257978422552411E-5	4.8257088788927326E-4	0.0	0.026505915680388757	5.677304563403215E-5
32-33	4.257978422552411E-5	5.251506721147974E-4	0.0	0.08374024231019742	6.386967633828617E-5
34-35	5.677304563403215E-5	5.748270870445755E-4	0.0	0.15808454556796253	7.096630704254018E-5
36-37	5.677304563403215E-5	6.245035019743537E-4	0.0	0.26735136852136165	7.096630704254018E-5
38-39	5.677304563403215E-5	6.245035019743537E-4	0.0	0.39279141284975566	7.096630704254018E-5
40-41	5.677304563403215E-5	6.386967633828617E-4	0.0	0.5073665155699368	7.096630704254018E-5
42-43	5.677304563403215E-5	6.599866554956237E-4	0.0	0.6882312456985548	7.096630704254018E-5
44-45	5.677304563403215E-5	7.096630704254019E-4	0.0	0.8590968231648788	7.096630704254018E-5
46-47	5.677304563403215E-5	7.380495932424179E-4	0.0	1.0511671331755137	7.096630704254018E-5
48-49	5.677304563403215E-5	7.380495932424179E-4	0.0	1.1822135157602685	7.096630704254018E-5
50-51	6.386967633828617E-5	8.444990538062282E-4	0.0	1.5093256114421532	7.096630704254018E-5
52-53	7.096630704254018E-5	8.515956845104822E-4	0.0	1.9294816322875123	9.225619915530224E-5
54-55	7.096630704254018E-5	8.728855766232443E-4	0.0	2.473920946656472	1.135460912680643E-4
56-57	7.096630704254018E-5	8.941754687360064E-4	0.0	3.075417171887634	1.135460912680643E-4
58-59	7.096630704254018E-5	9.225619915530224E-4	0.0	3.6985510241360666	1.135460912680643E-4
60-61	7.096630704254018E-5	9.367552529615305E-4	0.0	4.305632297731477	1.135460912680643E-4
62-63	8.515956845104823E-5	9.367552529615305E-4	0.0	4.759234739085985	1.135460912680643E-4
64-65	8.515956845104823E-5	0.0010786878670466108	0.0	4.782362658551149	1.135460912680643E-4
66-67	8.515956845104823E-5	0.0010999777591593728	0.0	4.789842507313432	1.135460912680643E-4
68-69	8.515956845104823E-5	0.001149654174089151	0.0	4.79605915581036	1.135460912680643E-4
70-71	9.225619915530224E-5	0.0011851373276104212	0.0	4.801906779510665	1.135460912680643E-4
72-73	9.935282985955626E-5	0.0012277171118359451	0.0	4.807009256987023	1.135460912680643E-4
74-75	9.935282985955626E-5	0.0012490070039487071	0.0	4.8119981883721135	1.135460912680643E-4
76-77	9.935282985955626E-5	0.0012773935267657234	0.0	4.8154684407864945	1.135460912680643E-4
78-79	9.935282985955626E-5	0.0012773935267657234	0.0	4.817320661400304	1.135460912680643E-4
80-81	9.935282985955626E-5	0.0012773935267657234	0.0	4.81826451328397	1.135460912680643E-4
82-83	9.935282985955626E-5	0.0012844901574699773	0.0	4.818967079723691	1.135460912680643E-4
84-85	9.935282985955626E-5	0.0013057800495827395	0.0	4.8193077179974955	1.135460912680643E-4
86-87	9.935282985955626E-5	0.0013483598338082635	0.0	4.8194212640887635	1.135460912680643E-4
88	9.935282985955626E-5	0.0014193261408508037	0.0	4.8194212640887635	1.135460912680643E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5815	0.0	38.24951	1
GTATCAA	9555	0.0	31.950144	1
TCAACGC	9790	0.0	29.661856	4
ATCAACG	9915	0.0	29.203064	3
CAACGCA	10260	0.0	28.302475	5
AACGCAG	10640	0.0	27.20933	6
TATCAAC	11350	0.0	26.168095	2
ACGCAGA	12820	0.0	22.54926	7
CGCAGAG	13325	0.0	21.661797	8
GTACATG	20975	0.0	19.607183	1
TACATGG	20655	0.0	19.17262	2
ACATGGG	20985	0.0	18.078539	3
GCAGAGT	16890	0.0	16.934715	9
GTGTAGA	10285	0.0	16.676594	94
CATGGGG	15165	0.0	16.618513	4
TAGCGCG	250	0.002621137	15.832501	94
AGAGTAC	14430	0.0	15.349306	10-11
CAGAGTA	16400	0.0	15.074073	10-11
TGTAGAT	10775	0.0	14.326393	94
TATACTG	4235	0.0	14.20768	5
>>END_MODULE