FastQCFastQC Report
Fri 27 May 2016
SRR2138851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305307
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134150.053.8705941
GTATCAA255400.038.9964181
ATCAACG316650.030.9992523
TCAACGC319250.030.7734954
CAACGCA323300.030.3616245
TATCAAC326500.030.2301672
CTTATAC72050.029.7058871
AACGCAG334100.029.3801656
ACGCAGA382950.025.6546237
CGCAGAG385250.025.5014618
GTGGTAT61250.023.667131
TATACAC113650.023.2144263
TGGTATC60250.022.5738892
GCAGAGT437600.022.4036459
GAGTACT249450.020.51383212-13
TTATACA115150.019.9571062
CAGAGTA430500.019.5452910-11
GTACATG321050.019.309211
AGAGTAC409900.019.22249410-11
TACATGG319650.019.0872
Produced by FastQC (version 0.11.5)