##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8305307
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94219695912505	34.0	31.0	34.0	31.0	34.0
2	33.05138786561412	34.0	33.0	34.0	31.0	34.0
3	33.110523066757196	34.0	33.0	34.0	31.0	34.0
4	36.472434191776415	37.0	37.0	37.0	35.0	37.0
5	36.439982411246206	37.0	37.0	37.0	35.0	37.0
6	36.47292773162991	37.0	37.0	37.0	35.0	37.0
7	36.43291741051836	37.0	37.0	37.0	35.0	37.0
8	36.41852889965416	37.0	37.0	37.0	35.0	37.0
9	38.20767697088139	39.0	39.0	39.0	37.0	39.0
10-11	38.16742788677168	39.0	39.0	39.0	37.0	39.0
12-13	38.137066998245814	39.0	39.0	39.0	37.0	39.0
14-15	39.59098760587658	41.0	40.0	41.0	37.0	41.0
16-17	39.572055554358194	41.0	40.0	41.0	37.0	41.0
18-19	39.598983637811344	41.0	40.0	41.0	37.0	41.0
20-21	39.586673149845424	41.0	40.0	41.0	37.0	41.0
22-23	39.5094129932807	41.0	39.0	41.0	37.0	41.0
24-25	39.35487226639224	41.0	39.0	41.0	36.0	41.0
26-27	39.35719912372025	41.0	39.0	41.0	36.0	41.0
28-29	39.27703888102014	41.0	39.0	41.0	36.0	41.0
30-31	39.17922034483285	41.0	39.0	41.0	36.0	41.0
32-33	38.97147187687526	40.0	39.0	41.0	35.0	41.0
34-35	38.88930399054959	40.0	38.5	41.0	35.0	41.0
36-37	39.07514039958336	40.5	39.0	41.0	35.0	41.0
38-39	39.14198962627407	41.0	39.0	41.0	35.0	41.0
40-41	39.128095622375895	41.0	39.0	41.0	35.0	41.0
42-43	38.98989685576825	41.0	39.0	41.0	35.0	41.0
44-45	39.01582998270346	41.0	39.0	41.0	35.0	41.0
46-47	38.971041855128675	40.0	39.0	41.0	35.0	41.0
48-49	38.91593825912672	40.0	39.0	41.0	35.0	41.0
50-51	38.845859419100954	40.0	38.5	41.0	35.0	41.0
52-53	38.737512872942546	40.0	38.0	41.0	35.0	41.0
54-55	38.61763324083705	40.0	38.0	41.0	35.0	41.0
56-57	38.47766309278376	40.0	38.0	41.0	34.0	41.0
58-59	38.32293568507703	40.0	37.0	41.0	34.0	41.0
60-61	38.153824762497294	40.0	37.0	41.0	34.0	41.0
62-63	37.94277512857749	39.5	36.0	41.0	34.0	41.0
64-65	37.690618280934515	39.0	36.0	41.0	33.5	41.0
66-67	37.389516201558436	39.0	35.0	40.5	33.0	41.0
68-69	37.076449898681005	38.0	35.0	40.0	33.0	41.0
70-71	36.73271366257855	37.5	35.0	40.0	33.0	41.0
72-73	36.40142388098393	37.0	35.0	39.5	32.0	41.0
74-75	36.062772505851974	36.5	35.0	39.0	32.0	41.0
76-77	35.19510329357984	35.5	34.0	38.0	31.0	39.0
78-79	35.40686430484364	35.5	34.5	37.5	32.0	39.0
80-81	35.18031179828831	35.0	34.5	37.0	32.0	39.0
82-83	34.90089136229909	35.0	34.0	37.0	32.0	39.0
84-85	34.62846694824944	35.0	34.0	36.0	31.0	37.5
86-87	34.400111560003936	35.0	34.0	36.0	31.0	37.0
88-89	34.19131992875986	35.0	34.0	35.5	31.0	37.0
90-91	33.98481199824641	35.0	34.0	35.0	31.0	36.0
92-93	33.846290379328195	35.0	34.0	35.0	31.0	36.0
94-95	33.76409956636812	35.0	34.0	35.0	31.0	36.0
96-97	33.7312170927288	35.0	34.0	35.0	31.0	36.0
98-99	33.746094672159856	35.0	34.0	35.0	31.0	35.0
100-101	32.36719978439734	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	6.0
20	22.0
21	119.0
22	634.0
23	2104.0
24	5586.0
25	11594.0
26	20540.0
27	33477.0
28	49783.0
29	67688.0
30	89105.0
31	114783.0
32	147221.0
33	196903.0
34	286446.0
35	431467.0
36	762512.0
37	1731417.0
38	2734395.0
39	1527866.0
40	91634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.16827858820356	21.37224340346321	15.683927321151964	23.775550687181266
2	13.368411696181406	21.772440532912974	42.095577076686936	22.763570694218682
3	16.6440124715468	27.12941908816112	31.639981915173493	24.586586525118584
4	11.481769784493165	19.33261933186078	39.471109128442606	29.714501755203454
5	12.586967004824027	38.32587725556135	35.788113741759034	13.299041997855587
6	28.412137975409934	38.98304288848598	19.232704971978155	13.372114164125923
7	25.512546082008818	33.61759752986707	23.157496259618256	17.712360128505864
8	24.19114745812078	36.801154642730324	21.393905760607172	17.613792138541715
9	25.25246626598925	17.113258717238264	21.503231442982937	36.13104357378955
10-11	23.482854004244157	27.5506147786358	29.010855890748882	19.955675326371157
12-13	24.652503793809416	25.085895385077002	29.21088463141005	21.050716189703536
14-15	21.761464412457986	26.427432676834776	27.228141008710683	24.58296190199655
16-17	20.896359561969152	29.751030832038627	27.81706223810792	21.5355473678843
18-19	20.867109427743006	28.482800214368957	30.04190573569406	20.608184622193978
20-21	21.97027789258344	27.420310237176626	29.974018968470872	20.635392901769066
22-23	21.568132201796026	27.573096681149156	29.801476820317625	21.05729429673719
24-25	21.347229105606946	27.765682424616152	29.874352719286573	21.012735750490336
26-27	21.265364514834314	27.96040768567038	29.81829534449974	20.955932454995562
28-29	21.03009850159268	27.99844105854205	29.86284048033584	21.108619959529435
30-31	21.565529872054086	27.736069099279582	29.813751856824926	20.884649171841403
32-33	21.14947861685763	27.97947068853611	29.754264498488013	21.116786196118248
34-35	21.269637767127442	28.06888354852935	29.82077858990485	20.840700094438358
36-37	21.151600609352077	28.018515744042638	29.73668851886294	21.09319512774234
38-39	21.069186917361066	27.866396848690062	29.868331710941554	21.196084523007322
40-41	21.328434567964543	27.7911288700156	29.739425441857804	21.141011120162048
42-43	21.43428296466126	27.817615764173027	29.50107173990823	21.24702953125749
44-45	21.364822152535396	28.122541606379027	29.211887746259045	21.300748494826532
46-47	21.549484397254854	27.993108455968592	28.78787216898705	21.669534977789503
48-49	21.4736540486711	28.14325857877978	28.732573091878482	21.650514280670638
50-51	21.422176555840732	28.28740680919043	28.64164819159129	21.64876844337755
52-53	21.572157108417315	28.261967097431757	28.61383134523845	21.55204444891248
54-55	21.596111009466252	28.250869063539803	28.45734865240761	21.695671274586335
56-57	21.713073052220565	28.45203366374063	28.200216511729042	21.634676772309763
58-59	21.499370645049808	28.61747049861158	28.467717765682554	21.415441090656056
60-61	21.718994808095523	28.674360555659838	28.196743841793943	21.4099007944507
62-63	21.525945038946677	28.822172422212766	28.162702268202146	21.48918027063841
64-65	21.524736056011477	28.737588055323897	28.173925647740035	21.56375024092459
66-67	21.65606883297143	28.60365155387306	28.08206238334552	21.65821722980999
68-69	21.68832154418616	28.479334206503143	28.162564307863203	21.669779941447498
70-71	21.65945131210898	28.303741530070507	28.24760689757217	21.78920026024834
72-73	21.701447404134917	28.28375180041794	28.247352065852926	21.76744872959422
74-75	21.631628349901057	28.337091798660374	28.206747658232782	21.82453219320578
76-77	21.680254343649143	28.298208586539342	28.124053813598938	21.897483256212574
78-79	21.691857143004373	28.326046138346133	28.10480957813774	21.877287140511754
80-81	21.71653623223777	28.296128219613202	28.14168441078898	21.845651137360043
82-83	21.756909443784135	28.251433041960674	28.218281252350792	21.773376261904403
84-85	21.836667980385016	28.21864734357557	28.15594473014798	21.78873994589143
86-87	21.70952702884049	28.23469888646396	28.190987077100356	21.864787007595197
88-89	21.800844397066495	28.130343250738793	28.17604691911198	21.89276543308273
90-91	21.775703588032794	28.15032749326677	28.264597208913493	21.809371709786944
92-93	21.826293183196363	28.04252814288289	28.274049563533648	21.8571291103871
94-95	21.70128085848802	28.133398637922042	28.275195852888046	21.890124650701893
96-97	21.678925273712228	28.13630348478165	28.336455808712863	21.84831543279326
98-99	21.883634571498977	28.678007649699477	28.350574798847187	21.08778297995436
100-101	19.69685026370999	32.45699697318262	26.598047436565185	21.248105326542206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2519.0
1	2137.0
2	2647.0
3	3748.5
4	4943.5
5	7396.5
6	9572.0
7	11941.5
8	14154.0
9	14700.5
10	13429.5
11	12330.0
12	14112.5
13	19393.5
14	31764.5
15	48404.0
16	61426.5
17	67764.0
18	67135.0
19	61889.0
20	54615.0
21	47301.0
22	43218.0
23	44723.0
24	51997.0
25	64281.0
26	80511.0
27	99486.5
28	119360.5
29	139696.5
30	159698.0
31	179608.5
32	201021.5
33	222588.0
34	242922.5
35	260802.0
36	278150.0
37	292432.5
38	302060.0
39	310936.5
40	320248.0
41	327638.0
42	330288.0
43	332685.5
44	336441.5
45	341146.0
46	346446.0
47	346802.5
48	337314.5
49	324732.5
50	313327.5
51	297443.0
52	278347.0
53	259757.5
54	241613.0
55	222919.5
56	202164.5
57	179492.0
58	156561.0
59	134980.0
60	113661.0
61	91913.0
62	71937.5
63	55166.0
64	41496.5
65	30723.0
66	22869.5
67	17014.5
68	12516.0
69	8769.0
70	5993.0
71	4212.0
72	2945.0
73	2035.5
74	1418.5
75	973.5
76	608.0
77	385.0
78	246.5
79	161.0
80	111.0
81	73.0
82	52.0
83	39.0
84	31.0
85	21.5
86	15.5
87	12.0
88	6.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.408098821632963E-5
2	0.03085978639922642
3	2.408098821632963E-5
4	2.408098821632963E-5
5	2.408098821632963E-5
6	1.2040494108164814E-5
7	6.020247054082408E-5
8	6.501866818409E-4
9	4.695792702184278E-4
10-11	6.020247054082407E-6
12-13	6.020247054082407E-6
14-15	0.0019324993043604527
16-17	1.8060741162247223E-5
18-19	0.0
20-21	1.9265239421472084E-4
22-23	0.002022984109881272
24-25	7.105810577878238E-4
26-27	0.014848557662912923
28-29	0.31555977852895273
30-31	0.3169849126404424
32-33	0.3343382162662855
34-35	0.21605193541447215
36-37	0.07187977045963052
38-39	0.06331373083245831
40-41	0.026595121927172748
42-43	0.01014158119043295
44-45	0.013816302673479618
46-47	0.010046468239106704
48-49	0.0072332725934563125
50-51	0.0076262546785814305
52-53	0.003420270630539245
54-55	0.001258166945123476
56-57	0.00375378649995625
58-59	0.004388019346763963
60-61	0.0030487821293178965
62-63	0.0018269983354618881
64-65	6.376618735520618E-4
66-67	0.007608903997668266
68-69	0.011329695685915332
70-71	0.011510203873601256
72-73	0.010028221975515414
74-75	0.010296862812161989
76-77	0.00932663787601561
78-79	0.002701614615926467
80-81	0.0022471084104803353
82-83	0.0018580303809782222
84-85	3.041600502350739E-5
86-87	0.0027838202827146366
88-89	0.0
90-91	0.008729762742082354
92-93	0.018314225890861128
94-95	0.0013311828777414047
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	119.0
20-21	415.0
22-23	1037.0
24-25	2550.0
26-27	3829.0
28-29	7088.0
30-31	15947.0
32-33	21327.0
34-35	30665.0
36-37	43075.0
38-39	51729.0
40-41	55855.0
42-43	68234.0
44-45	83732.0
46-47	83192.0
48-49	71243.0
50-51	61401.0
52-53	58436.0
54-55	62704.0
56-57	67932.0
58-59	68494.0
60-61	67991.0
62-63	68205.0
64-65	73984.0
66-67	80728.0
68-69	74544.0
70-71	69061.0
72-73	65946.0
74-75	66923.0
76-77	68800.0
78-79	72101.0
80-81	73376.0
82-83	71402.0
84-85	72558.0
86-87	76617.0
88-89	82179.0
90-91	88208.0
92-93	89107.0
94-95	100934.0
96-97	157034.0
98-99	702249.0
100-101	5224356.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.20926780300661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04992358960322	77.63137978376389
2	7.126301161081747	12.287064104811469
3	1.6553966541724117	4.281316004392518
4	0.5760437293882458	1.986412325322958
5	0.24839466245743577	1.0706960988315257
6	0.1188014726326566	0.6145072781748153
7	0.07353681243402191	0.4437688329552843
8	0.03959652531353524	0.2730869963858472
9	0.029867403938705777	0.2317362322259215
>10	0.08095115795163887	1.0372150256605963
>50	9.040970534246272E-4	0.051142057265164804
>100	2.2030448083641167E-4	0.04542648966417539
>500	4.1620284328230364E-5	0.02319655716479867
>1k	2.0809207784000594E-5	0.02305221338105822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.2040494108164816E-5	0.0	0.0	0.0
5	0.0	1.2040494108164816E-5	0.0	0.0	0.0
6	0.0	1.2040494108164816E-5	0.0	0.0	1.2040494108164816E-5
7	0.0	1.2040494108164816E-5	0.0	9.632395286531853E-5	1.2040494108164816E-5
8	0.0	2.4080988216329632E-5	0.0	9.632395286531853E-5	1.2040494108164816E-5
9	0.0	7.224296464898889E-5	0.0	1.3244543518981298E-4	4.8161976432659264E-5
10-11	0.0	7.224296464898889E-5	0.0	1.685669175143074E-4	6.020247054082408E-5
12-13	0.0	7.224296464898889E-5	0.0	1.685669175143074E-4	7.224296464898889E-5
14-15	0.0	8.42834587571537E-5	0.0	2.5285037627146114E-4	7.82632117030713E-5
16-17	0.0	8.42834587571537E-5	0.0	2.5887062332554354E-4	8.42834587571537E-5
18-19	0.0	9.030370581123611E-5	0.0	3.130528468122852E-4	8.42834587571537E-5
20-21	0.0	9.632395286531853E-5	0.0	3.431540820826972E-4	8.42834587571537E-5
22-23	0.0	1.0836444697348334E-4	0.0	3.6121482324494444E-4	9.030370581123611E-5
24-25	0.0	1.2040494108164815E-4	0.0	4.4549828200209817E-4	9.632395286531853E-5
26-27	0.0	1.2040494108164815E-4	0.0	5.89984211300076E-4	9.632395286531853E-5
28-29	0.0	1.2642518813573057E-4	0.0	0.0011017052108970805	9.632395286531853E-5
30-31	0.0	1.685669175143074E-4	0.0	0.0024442203039574577	9.632395286531853E-5
32-33	0.0	1.685669175143074E-4	0.0	0.005261695925268024	1.0836444697348334E-4
34-35	0.0	2.167288939469667E-4	0.0	0.00935546392204406	1.0836444697348334E-4
36-37	0.0	2.5285037627146114E-4	0.0	0.015357650234964222	1.1438469402756575E-4
38-39	0.0	2.5285037627146114E-4	0.0	0.023599368452003038	1.3244543518981298E-4
40-41	0.0	2.7091111743370836E-4	0.0	0.032208321739340885	1.4448592929797779E-4
42-43	0.0	3.010123527041204E-4	0.0	0.039914237968566364	1.4448592929797779E-4
44-45	0.0	3.9733630556943887E-4	0.0	0.04923358040828593	1.4448592929797779E-4
46-47	0.0	4.936602584347575E-4	0.0	0.05933555496503621	1.4448592929797779E-4
48-49	0.0	4.936602584347575E-4	0.0	0.06892580852218949	1.505061763520602E-4
50-51	0.0	5.598829760296639E-4	0.0	0.07757690353890591	1.565264234061426E-4
52-53	0.0	5.659032230837463E-4	0.0	0.08528884001518547	1.685669175143074E-4
54-55	0.0	5.719234701378287E-4	0.0	0.09401819824360497	1.8662765867655465E-4
56-57	0.0	5.779437171919111E-4	0.0	0.10046588283852723	1.9866815278471946E-4
58-59	0.0	5.779437171919111E-4	0.0	0.1059503279047963	2.1672889394696668E-4
60-61	0.0	5.960044583541584E-4	0.0	0.11104947715960409	2.2274914100104908E-4
62-63	0.0	6.140651995164056E-4	0.0	0.11594393801457309	2.287693880551315E-4
64-65	0.0	6.863081641653944E-4	0.0	0.12077217615194719	2.287693880551315E-4
66-67	0.0	7.103891523817241E-4	0.0	0.12613621627713462	2.287693880551315E-4
68-69	0.0	7.344701405980538E-4	0.0	0.13121730479078014	2.287693880551315E-4
70-71	0.0	7.344701405980538E-4	0.0	0.1361238061398573	2.347896351092139E-4
72-73	0.0	7.344701405980538E-4	0.0	0.139898501042767	2.5285037627146114E-4
74-75	0.0	7.465106347062186E-4	0.0	0.14295678654624086	2.5285037627146114E-4
76-77	0.0	7.766118699766306E-4	0.0	0.145744160932281	2.5285037627146114E-4
78-79	0.0	7.82632117030713E-4	0.0	0.14819440148329255	2.6489087037962595E-4
80-81	0.0	7.886523640847954E-4	0.0	0.14942855212937944	2.7091111743370836E-4
82-83	0.0	7.946726111388779E-4	0.0	0.14981384794084074	2.7693136448779076E-4
84-85	0.0	8.067131052470427E-4	0.0	0.15015700202292342	2.7693136448779076E-4
86-87	0.0	8.187535993552075E-4	0.0	0.15031352844632956	2.7693136448779076E-4
88-89	0.0	8.669155757878667E-4	0.0	0.15038577141097856	2.8897185859595557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13415	0.0	53.870594	1
GTATCAA	25540	0.0	38.996418	1
ATCAACG	31665	0.0	30.999252	3
TCAACGC	31925	0.0	30.773495	4
CAACGCA	32330	0.0	30.361624	5
TATCAAC	32650	0.0	30.230167	2
CTTATAC	7205	0.0	29.705887	1
AACGCAG	33410	0.0	29.380165	6
ACGCAGA	38295	0.0	25.654623	7
CGCAGAG	38525	0.0	25.501461	8
GTGGTAT	6125	0.0	23.66713	1
TATACAC	11365	0.0	23.214426	3
TGGTATC	6025	0.0	22.573889	2
GCAGAGT	43760	0.0	22.403645	9
GAGTACT	24945	0.0	20.513832	12-13
TTATACA	11515	0.0	19.957106	2
CAGAGTA	43050	0.0	19.54529	10-11
GTACATG	32105	0.0	19.30921	1
AGAGTAC	40990	0.0	19.222494	10-11
TACATGG	31965	0.0	19.087	2
>>END_MODULE